MAOA μ-VNTR多态性荧光标记自动分型方法的建立及应用
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摘要
目的应用毛细管电泳和荧光标记技术,建立MAOA μ-VNTR多态荧光标记自动分型的方法.方法使用Chelex-100法提取DNA后,采用荧光标记和毛细管电泳检测技术对720份云南汉族无关个体血样进行MAOA μ-VNTR基因分型.结果荧光毛细管电泳分型方法能准确、高效地对MAOA μ-VNTR多态性进行分型检测.结论 MAOA μ-VNTR多态性荧光标记自动分型方法操作简便,在针对MAOA μ-VNTR多态性的相关研究中具有良好的应用前景.
Objective To establish an automatic typing method for MAOA μ-VNTR polymorphism test using capillary electrophoresis and fluorescence labeling techniques. Me thods DNAwas extracted by Chelex-100 method. Blood samples from 720 unrelated Chinese Han individuals in Yunnan were genotyped by capillary electrophoresis and fluorescence labeled primer. Re s ults MAOA μ-VNTR polymorphism fluorescent labeling automatic typing method had high efficiency. And the results from this method were accurate and reliable.Conclus ions The operation of MAOA μ-VNTR polymorphism fluorescent labeling automatic typing method is simple. It has good prospects in the research for the MAOA μ-VNTR polymorphism test.
Objective To establish an automatic typing method for MAOA μ-VNTR polymorphism test using capillary electrophoresis and fluorescence labeling techniques. Me thods DNAwas extracted by Chelex-100 method. Blood samples from 720 unrelated Chinese Han individuals in Yunnan were genotyped by capillary electrophoresis and fluorescence labeled primer. Re s ults MAOA μ-VNTR polymorphism fluorescent labeling automatic typing method had high efficiency. And the results from this method were accurate and reliable.Conclus ions The operation of MAOA μ-VNTR polymorphism fluorescent labeling automatic typing method is simple. It has good prospects in the research for the MAOA μ-VNTR polymorphism test.
引文
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[11]SAWYER A M,BORDUIN C M,DOPP A R.Long-term effects of prevention and treatment on youth antisocial behavior:A meta-analysis[J].Clinical psychology review,2015,42(7):130-144.
[12]张洁旭,陈彦博,张克让,等.单胺氧化酶A启动子可变数目重复序列多态性与中国北方汉族人群重性抑郁症的关联分析[J].中国医学科学院学报,2009,31(6):728-733.
[2]SAMOCHOWIEC A,CHEC M,KOPACZEWSKA E,et al.Monoamine oxidase a promoter variable number of tandem repeats(MAOA-u VNTR)in alcoholics according to Lesch typology[J].International journal of environmental research and public health,2015,12(3):3317-3326.
[3]LAQUA C,ZILL P,KOLLER G,et al.Association between the MAOA-u VNTR polymorphism and antisocial personality traits in alcoholic men[J].Fortschritte der Neurologie-Psychiatrie,2015,83(3):162-169.
[4]KIMSK,PARK HJ,SEOK H,et al.Association study between monoamine oxidase A(MAOA)gene polymorphisms and schizophrenia:lack of association with schizophrenia and possible association with affective disturbances of schizophrenia[J].Molecular biology reports,2014,41(5):3457-3464.
[5]ROZYCKA A,SLOPIEN R,SLOPIEN A,et al.The MAOA,COMT,MTHFR and ESR1 gene polymorphisms are associated with the risk of depression in menopausal women[J].Maturitas,2016,84(9):42-54.
[6]GUO H,REN Y,ZHAO N,et al.Synergistic effect of 5-HT2A receptor gene and MAOA gene on the negative emotion of patients with depression[J].Clinical physiology and functional imaging,2014,34(4):277-281.
[7]ZHANG W,CAO C,WANG M,et al.Monoamine oxidase a(MAOA)and catechol-O-Methyltransferase(COMT)gene polymorphisms interact with maternal parenting in association with adolescent reactive aggression but not proactive aggression:evidence of differential susceptibility[J].Journal of youth and adolescence,2016,45(4):812-829.
[8]OUELLET-MORIN I,COTE S M,VITARO F,et al.Effects of the MAOA gene and levels of exposure to violence on antisocial outcomes[J].The British journal of psychiatry:the journal of mental science,2016,208(1):42-48.
[9]XIE B,LI D,LONDON S J,PALMER P H,et al.Gender difference in interactions between MAOA promoter u VNTR polymorphism and negative familial stressors on body mass index among Chinese adolescents[J].Pediatric obesity,2014,9(5):80-90.
[10]HABERSTICK B C,LESSEM J M,HEWITT J K,et al.MAOA genotype,childhood maltreatment,and their interaction in the etiology of adult antisocial behaviors[J].Biological psychiatry,2014,75(1):25-30.
[11]SAWYER A M,BORDUIN C M,DOPP A R.Long-term effects of prevention and treatment on youth antisocial behavior:A meta-analysis[J].Clinical psychology review,2015,42(7):130-144.
[12]张洁旭,陈彦博,张克让,等.单胺氧化酶A启动子可变数目重复序列多态性与中国北方汉族人群重性抑郁症的关联分析[J].中国医学科学院学报,2009,31(6):728-733.