量子点/多孔硅光子晶体生物传感器用于检测链霉亲和素
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摘要
本文主要研究了基于量子点/多孔硅光子晶体制备生物传感器的可行性及其在生物检测中的实际应用.功能化多孔硅光子晶体器件与链霉亲和素连接形成生物特征元件.固定浓度的量子点标记的生物素与链霉亲和素发生特异性反应.多孔硅光子晶体的高反射带隙位于量子点发射荧光峰处,使得量子点的荧光信号得到放大.多孔硅光子晶体传感器的检测灵敏度明显提高,使检测限达到pM量级.
We mainly investigate the feasibility of preparing a biosensor based on quantum dots/porous silicon photonic crystal and the practical application in biological detection. Functionalized porous silicon photonic crystal devices were connected with target streptavidin to form a biometric element. Quantum dots labeled biotin at a ?xed concentration speci?cally reacted with streptavidin. High-re?ective bandgap of porous silicon photonic crystal, which located at the emission ?uorescence peak of quantum dots, is bene?cial to magnify the emission ?uorescence signal of quantum dots. Porous silicon photonic crystal sensor revealed a marked enhancement of the detection sensitivity, so that the detection limit is reached pM.
We mainly investigate the feasibility of preparing a biosensor based on quantum dots/porous silicon photonic crystal and the practical application in biological detection. Functionalized porous silicon photonic crystal devices were connected with target streptavidin to form a biometric element. Quantum dots labeled biotin at a ?xed concentration speci?cally reacted with streptavidin. High-re?ective bandgap of porous silicon photonic crystal, which located at the emission ?uorescence peak of quantum dots, is bene?cial to magnify the emission ?uorescence signal of quantum dots. Porous silicon photonic crystal sensor revealed a marked enhancement of the detection sensitivity, so that the detection limit is reached pM.
引文
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[27]Focsan M,Campu A,Craciun A M,et al.A simple and efficient design to improve the detection of biotin-streptavidin interaction with plasmonic nanobiosensors[J].Biosensors&Bioelectronics,2016,86:728-735.
[28]He Y,Jiao B N.DNA covalently linked to graphene oxide for biotin-streptavidin interaction assay[J].Talanta,2017,163:140-145.
[29]Freitag S,Le T I,Klumb L,et al.Structural studies of the streptavidin binding loop[J].Protein Science,1997,6(6):1157-1166.
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[31]Stefano L D,Arcari P,Lamberti A,et al.DNA Optical Detection Based on Porous Silicon Technology:from Biosensors to Biochips[J].Sensors,2007,7(2):214-221.
[32]Zhang H,Jia Z,Lv X,et al.Porous silicon optical microcavity biosensor on silicon-on-insulator wafer for sensitive DNAdetection[J].Biosensors&Bioelectronics,2013,44(1):89-94.
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[2]Snow P A,Squire E K,Russell P S J,et al.Vapor Sensing Using the Optical Properties of Porous Silicon Bragg Mirrors[J].Journal of Applied Physics,1999,86(4):1781-1785.
[3]Saarinen J,Weiss S,Fauchet P,et al.Optical Sensor Based on Resonant Porous Silicon Structures[J].Optics Express,2005,13(10):3754-3764.
[4]Yakovleva M,Bhand S,Danielsson B.The enzyme thermistor-a realistic biosensor concept[J].Analytica Chimica Acta,2013,766(5):1-12.
[5]Gaur G,Koktysh D S,Weiss S M.Immobilization of Quantum Dots in Nanostructured Porous Silicon Films:Characterizations and Signal Amplification for Dual-Mode Optical Biosensing[J].Advanced Functional Materials,2013,23(29):3604-3614.
[6]马成文,李鹏,贾振红,等.一种基于多孔硅多层布喇格反射镜生物监测新方法的研究[J].新疆大学学报(自然科学版),2018,35(2):172-176.
[7]Pacholski C.Photonic Crystal Sensors Based on Porous Silicon[J].Sensors,2013,13(4):4694-4713.
[8]Donato M G,Monaca M A,Faggio G,et al.Optical trapping of porous silicon nanoparticles[J].Nanotechnology,2011,22(50):505704.
[9]Acikgoz S,Sarpkaya I,Milas P,et al.Investigation of fluorescence dynamics of bodipy embedded in porous silicon and monitoring formation of a sio2 layer via a confocal flim-based nset method[J].Journal of Physical Chemistry C,2011,115(45):22186-22190.
[10]Stepikhova M,Palmetshofer L,Jantsch W,et al.1.5 mu m infrared photoluminescence phenomena in Er-doped porous silicon[J].Applied Physics Letters,1999,74(4):537-539.
[11]Jacobsohn L G,Bennett B L,Cooke D W.The effects of ion irradiation on porous silicon photoluminescence[J].Journal of Applied Physics,2005,97(3):033528.
[12]Naddaf M,Al-Marini A.Porous silicon as a platform for immobilization of biotin anti-human IL-6;rat IgG2a antibody onto p-type porous silicon via physical absorption method[J].Sensors and actuators b-chemical,2011,160(1):835-839.
[13]Rossi A M,Wang L,Reipa V.Porous silicon biosensor for detection of viruses[J].Biosensors&Bioelectronics,2007,23(5):741-745.
[14]Worsfold O,Voelcker N H,Nishiya T.Biosensing using lipid bilayers suspended on porous silicon[J].Langmuir,2006,22(16):7078-7083.
[15]Levitsky I A,Euler W B,Tokranova N,et al.Fluorescent polymer-porous silicon microcavity devices for explosive detection.Applied Physics Letters[J].Applied Physics Letters,2007,90(4):1781.
[16]Ong P L,and Levitsky I A.Fluorescent gas sensors based on nanoporous optical resonators(microcavities)infiltrated with sensory emissive polymers[J].IEEE Sensors Journal,2011,11(11):2947-2951.
[17]Liu C,Jia Z,Lv X,et al.Enhancement of QDs’fluorescence based on porous silicon Bragg mirror[J].Physica B,2015,457(41):263-268.
[18]Palestino A G,Del M M B,Del Rio J A.Fluorescence tuning of confined molecules in porous silicon mirrors[J].Applied Physics Letters,2007,91(12):121909.
[19]Ledentsov N N,Ustinov V M,Shchukinn V A,et al.Quantum dot heterostructures:Fabrication,properties,lasers(Review)[J].Semiconductors,1998,32(4):343-365.
[20]Midgett A G,Hillhouse H W,Hughes B K,et al.Flowing versus Static Conditions for Measuring Multiple Exciton Generation in PbSe Quantum Dots[J].Journal of Physical Chemistry C,2010,114(41):17486-17500.
[21]Lord S J,Lee H L,Moerner W E.Single-molecule spectroscopy and imaging of biomolecules in living cells[J].Analytical chemistry,2010,82(6):2192-203.
[22]Gaur G,Koktysh D S,Fleetwood D M.Influence of interfacial oxide on the optical properties of single layer CdTe/CdSquantum dots in porous silicon scaffolds[J].Applied Physics Letters,2015,107(6):063106.
[23]Chia-Man C,Hsing-Tzu C,Hsiao V K S.Quantum dot-doped porous silicon metal-semiconductor metal photodetector[J].Nano Express,2012,7(1):1-4.
[24]Yang F,Cunningham B T.Enhanced quantum dot optical down-conversion using asymmetric 2D photonic crystals[J].Optics Express,2011,19(5):3908-3918.
[25]Elo H A,R¨ais¨anen S,Tuohimaa P J.Induction of an antimicrobial biotin-binding egg white protein(avidin)in chick tissues in septic Escherichia coli,infection[J].Experientia,1980,36(3):312-313.
[26]Saremi B,Wei M Y,Liu Y.Re-evaluation of biotin-streptavidin conjugation in Forster resonance energy transfer applications[J].Journal of biomedical optics,2014,19(8):085008.
[27]Focsan M,Campu A,Craciun A M,et al.A simple and efficient design to improve the detection of biotin-streptavidin interaction with plasmonic nanobiosensors[J].Biosensors&Bioelectronics,2016,86:728-735.
[28]He Y,Jiao B N.DNA covalently linked to graphene oxide for biotin-streptavidin interaction assay[J].Talanta,2017,163:140-145.
[29]Freitag S,Le T I,Klumb L,et al.Structural studies of the streptavidin binding loop[J].Protein Science,1997,6(6):1157-1166.
[30]Li Y Y,Jia Z H,Wang J J,et al.Biological reaction signal enhancement in porous silicon bragg mirror based on quantum dots fluorescence[J].Optoelectronics Letters,2017,13(3):172-174.
[31]Stefano L D,Arcari P,Lamberti A,et al.DNA Optical Detection Based on Porous Silicon Technology:from Biosensors to Biochips[J].Sensors,2007,7(2):214-221.
[32]Zhang H,Jia Z,Lv X,et al.Porous silicon optical microcavity biosensor on silicon-on-insulator wafer for sensitive DNAdetection[J].Biosensors&Bioelectronics,2013,44(1):89-94.
[33]Rong G,Weiss S M.Optical DNA sensing based on resonant porous silicon structures[J].Proceedings of SPIE-The International Society for Optical Engineering,2007,6477:1-11.