甘露糖结合凝集素基因多态性与结核病易感性关系的研究
|
摘要
目的了解甘露糖结合凝集素基因多态性与新疆喀什地区结核病易感性的关系。方法选取2015年12月~2017年12月在新疆喀什地区收集肺结核患者193例血液样本,收集同时期健康体检人员142例血液样本,采用试剂盒法提取全血基因组DNA,采用英国LGC公司生产的基于竞争性等位基因特异性PCR(KASP)技术的SNPline基因分型平台对选定的MBL基因SNP位点rs7096206进行分型,获得该位点的基因型频率和等位基因频率。结果 MBL基因rs7096206位点的三个基因型CC、GC、GG在结核患者组和对照组中的分布,差异无统计学意义(P>0.05),但从等位基因在两组间的分布上看,突变型碱基G在结核患者组中的频率高于对照组,差异有统计学意义(P<0.05)。结论 MBL基因rs7096206多态性位点与新疆结核病易感性存在关联,携带G碱基比携带C碱基具有更高的结核病发病风险。
Objective To investigate the relationship between mannose-binding lectin gene polymorphism and susceptibility to tuberculosis in Kashgar,Xinjiang.Methods From December 2015 to December 2017,193 blood samples of pulmonary tuberculosis patients were collected in Kashgar,Xinjiang.142 blood samples were collected from concurrent health checkups.The whole blood genomic DNA was extracted by kit method,and the selected SNP locus rs7096206 was obtained by SNPline genotyping platform based on competitive allele-specific PCR(KASP)technology produced by LGC,UK.Genotypic frequency and allele frequency of this locus.Results There were no significant differences in the distribution of three genotypes CC,GC and GG of MBL gene rs7096206 in tuberculosis patients and control group(P>0.05),but the distribution of alleles between the two groups was observed.The frequency of mutant base G in the tuberculosis group was higher than that in the control group,and the difference was statistically significant( P<0.05).Conclusion The polymorphism of MBL gene rs7096206 is associated with susceptibility to tuberculosis in Xinjiang,and carrying G bases has a higher risk of tuberculosis than carrying C bases.
Objective To investigate the relationship between mannose-binding lectin gene polymorphism and susceptibility to tuberculosis in Kashgar,Xinjiang.Methods From December 2015 to December 2017,193 blood samples of pulmonary tuberculosis patients were collected in Kashgar,Xinjiang.142 blood samples were collected from concurrent health checkups.The whole blood genomic DNA was extracted by kit method,and the selected SNP locus rs7096206 was obtained by SNPline genotyping platform based on competitive allele-specific PCR(KASP)technology produced by LGC,UK.Genotypic frequency and allele frequency of this locus.Results There were no significant differences in the distribution of three genotypes CC,GC and GG of MBL gene rs7096206 in tuberculosis patients and control group(P>0.05),but the distribution of alleles between the two groups was observed.The frequency of mutant base G in the tuberculosis group was higher than that in the control group,and the difference was statistically significant( P<0.05).Conclusion The polymorphism of MBL gene rs7096206 is associated with susceptibility to tuberculosis in Xinjiang,and carrying G bases has a higher risk of tuberculosis than carrying C bases.
引文
[1]Wu L,Deng H,Zheng Y,et al.An association study of NRAMP1,VDR,MBL and their interaction with the susceptibility to tuberculosis in a Chinese population[J].Internation J ournal Infectious Diseases,2015,38(C):129-135.
[2]Hijikata M,Matsushita I,Hang NT,et al.Age dependent association of mannose binding lectin polymorphisms with the development of pulmonary tuberculosis in Viet Mam[J].Hum Immunol,2014,75(8):840-846.
[3]中华医学会.临床诊疗指南:结核病分册[M].北京:人民卫生出版社,2005.
[4]Garred P,Genster N,Pilely K,et al.A journey through the lectin pathway of complement-MBL and beyond[J].Immunol Rev,2016,274(1):74-97.
[5]杨春娥,冯喜英,久太.结核病易感基因的研究现状[J].中华肺部疾病杂志,2016,9(2):207-209.
[6]刘洋,郭艳玲,孙琦,等.甘露醇结合凝集素基因多态性与肺结核易感性关系的研究[J].中国肺科杂志,2015,20(6):971-974.
[7]Chen M,Deng J,Su C,et al.Impact of passive smoking,cooking with solid fuel exposure,and MBL MASP-2 gene polymorphism up-on susceptibility to tuberculosis[J].Int J Infect Dis,2014(29):1-6.
[8]李宇,吴芳,章乐,等.MBL基因多态性与新疆汉族人群结核病易感性的研究[J].中国人兽共患病学报,2011,27(9):769-773.
[9]陈梦施.湖南省汉族人群MBL与MASP2基因多态性与相关因素对结核易感性的综合影响研究[D].中南大学,2014:1-112.
[2]Hijikata M,Matsushita I,Hang NT,et al.Age dependent association of mannose binding lectin polymorphisms with the development of pulmonary tuberculosis in Viet Mam[J].Hum Immunol,2014,75(8):840-846.
[3]中华医学会.临床诊疗指南:结核病分册[M].北京:人民卫生出版社,2005.
[4]Garred P,Genster N,Pilely K,et al.A journey through the lectin pathway of complement-MBL and beyond[J].Immunol Rev,2016,274(1):74-97.
[5]杨春娥,冯喜英,久太.结核病易感基因的研究现状[J].中华肺部疾病杂志,2016,9(2):207-209.
[6]刘洋,郭艳玲,孙琦,等.甘露醇结合凝集素基因多态性与肺结核易感性关系的研究[J].中国肺科杂志,2015,20(6):971-974.
[7]Chen M,Deng J,Su C,et al.Impact of passive smoking,cooking with solid fuel exposure,and MBL MASP-2 gene polymorphism up-on susceptibility to tuberculosis[J].Int J Infect Dis,2014(29):1-6.
[8]李宇,吴芳,章乐,等.MBL基因多态性与新疆汉族人群结核病易感性的研究[J].中国人兽共患病学报,2011,27(9):769-773.
[9]陈梦施.湖南省汉族人群MBL与MASP2基因多态性与相关因素对结核易感性的综合影响研究[D].中南大学,2014:1-112.