MBL启动子区-221位点的多态性和血清MBL、白细胞介素17水平与儿童支气管炎伴喘息的相关性研究
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摘要
目的探讨甘露糖结合凝集素(MBL)启动子区-221位点的多态性及血清MBL、白细胞介素(IL)-17在支气管炎伴喘息儿童诊断中的价值。方法将研究对象分为既往喘息1次(喘1组)、既往喘息2次(喘2组)和健康对照组,每组各30例,通过聚合酶链反应-限制性片段长度多态性技术检测MBL启动子区-221位点的多态性,同时用酶联免疫吸附试验测定血清MBL、IL-17水平。结果 MBL启动子区-221位点的CC基因型频率在健康对照组最高,喘2组最低,3组之间比较差异无统计学意义(P>0.05);GG基因型频率在健康对照组中最低,喘1组、喘2组的频率相同,3组之间比较差异无统计学意义(P>0.05)。血清MBL在健康对照组中最高,喘1组次之,喘2组最低,差异有统计学意义(P<0.05);血清中IL-17在喘1组和喘2组水平高于健康对照组,血清IL-17在喘2组中高于喘1组,差异有统计学意义(P<0.05)。结论 MBL启动子区-221位点的基因型频率多态性与儿童喘息无相关性;血清MBL、IL-17的检测在喘息儿童的诊断中具有一定的临床意义。
Objective To investigate the polymorphism of locus MBL promoter region-221 and the value of serum mannose-binding lectin(MBL)and interleukin-17(IL-17)in the diagnosis of children with asthmatic bronchitis.Methods The subjects were divided into 3 groups:the first wheezing group(group 1),the second wheezing group(group 2)and the control group,30 cases in each group.Polymorphism at position of MBL promoter region-221 by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)and serum MBL and IL-17 levels were detected by enzyme-linked immunosorbent assay(ELISA).ResultsThe genotype frequency of CC genotype at MBL promoter region-221 was highest in the healthy control group and lowest in group 2,there was no significant difference among the three groups(P>0.05).The frequency of GG genotype frequency was lowest in healthy control group,the frequency of group 1 and group 2 were the same,there was no significant difference among three groups(P>0.05).Serum MBL was highest in the healthy control group,followed by the group 1 and the lowest in the group 2.The difference was statistically significant(P<0.05).The levels of serum IL-17 in the group 1 and group 2 were higher than those of the healthy control group,serum IL-17 in group 2 was higher than group 1,the difference was statistically significant(P<0.05).Conclusion Genotype frequency polymorphism of the locus in MBL promoter region-221 is not associated with childhood wheezing.The detection of serum MBL and IL-17 has certain clinical significance in the diagnosis of children with asthmatic bronchitis.
Objective To investigate the polymorphism of locus MBL promoter region-221 and the value of serum mannose-binding lectin(MBL)and interleukin-17(IL-17)in the diagnosis of children with asthmatic bronchitis.Methods The subjects were divided into 3 groups:the first wheezing group(group 1),the second wheezing group(group 2)and the control group,30 cases in each group.Polymorphism at position of MBL promoter region-221 by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)and serum MBL and IL-17 levels were detected by enzyme-linked immunosorbent assay(ELISA).ResultsThe genotype frequency of CC genotype at MBL promoter region-221 was highest in the healthy control group and lowest in group 2,there was no significant difference among the three groups(P>0.05).The frequency of GG genotype frequency was lowest in healthy control group,the frequency of group 1 and group 2 were the same,there was no significant difference among three groups(P>0.05).Serum MBL was highest in the healthy control group,followed by the group 1 and the lowest in the group 2.The difference was statistically significant(P<0.05).The levels of serum IL-17 in the group 1 and group 2 were higher than those of the healthy control group,serum IL-17 in group 2 was higher than group 1,the difference was statistically significant(P<0.05).Conclusion Genotype frequency polymorphism of the locus in MBL promoter region-221 is not associated with childhood wheezing.The detection of serum MBL and IL-17 has certain clinical significance in the diagnosis of children with asthmatic bronchitis.
引文
[1]中华医学会儿科分会呼吸学组,《中华儿科杂志》编辑委员会.儿童支气管哮喘诊断与防治指南(2016年版)[J].中华儿科杂志,2016,54(3):167-181.
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[6]陈梦施.湖南省汉族人群MBL与MASP2基因多态性与相关因素对结核易感性的综合影响研究[D].长沙:中南大学,2014.
[7]卞芳芳,张冲林,甄清,等.瘦素、IL-17水平与婴幼儿喘息患儿哮喘预测指数的相关性[J].临床肺科杂志,2016,21(10):1853-1855.
[8]赵冰,潘家华.学龄前儿童反复喘息相关危险因素Logistic回归分析[J].安徽医科大学学报,2015,50(8):1154-1156.
[9]SHI J,ZHU X,XIE M,et al.MBL2polymorphisms and the risk of asthma:a meta-analysis[J].Ann Allerg Asthma Im,2016,117(4):417-422.
[10]TAKAHASHI K.Mannose-binding lectin and the balance between immune protection and complication[J].Expert Rev Anti Infect Ther,2011,9(12):1179-1190.
[11]NEGI V S,DEVARAJU P,MISRA D P,et al.Mannosebinding lectin(MBL)codon 54(rs1800450)polymorphism predisposes towards medium vessel vasculitis in patients with systemic lupus erythematosus[J].Clin Rheumatol,2017,36(4):837-843.
[12]WANG M,CHEN Y,ZHANG Y,et al.Mannan-binding lectin directly interacts with Toll-like receptor 4and suppresses lipopolysaccharide-induced inflammatory cytokine secretion from THP-1cells[J].Cell Mol Immunol,2011,8(3):265-275.
[13]SORBELLO V,CIPRANDI G,STEFANO A D,et al.Nasal IL-17Fis related to bronchial IL-17F/neutrophilia and exacerbations in stable atopic severe asthma[J].Allergy,2015,70(2):236-240.
[14]张印,崔艺馨,杨明会.呼吸道变应性炎症相关Th细胞因子的研究进展[J].中国医药导报,2016,13(16):31-34.
[15]WHITEHEAD G S,WILSON R H,NAKANO K,et al.IL-35Production by ICOS+Tregs Reverses Established IL-17-dependent Allergic Airways Disease[J].J Allergy Clin Immun,2012,129(1):207.
[2]温楚玲.甘露聚糖结合凝集素与糖尿病的研究进展[J].国际检验医学杂志,2016,37(4):512-514.
[3]AHMADIKIA K,KORDBACHEH P,SHADPOUR P,et al.Increased urine interleukin(IL)-17and IL-22in patients with Candida urinary tract infection[J].Iran J Kidney Dis,2018,12(1):33-39.
[4]胡亚美,江载芳.诸福棠实用儿科学(第8版)[M].北京:人民卫生出版社,2015:626.
[5]胡亚美,江载芳,申坤玲,等.诸福棠实用儿科学(第7版)[M].北京:人民卫生出版社,2005:635.
[6]陈梦施.湖南省汉族人群MBL与MASP2基因多态性与相关因素对结核易感性的综合影响研究[D].长沙:中南大学,2014.
[7]卞芳芳,张冲林,甄清,等.瘦素、IL-17水平与婴幼儿喘息患儿哮喘预测指数的相关性[J].临床肺科杂志,2016,21(10):1853-1855.
[8]赵冰,潘家华.学龄前儿童反复喘息相关危险因素Logistic回归分析[J].安徽医科大学学报,2015,50(8):1154-1156.
[9]SHI J,ZHU X,XIE M,et al.MBL2polymorphisms and the risk of asthma:a meta-analysis[J].Ann Allerg Asthma Im,2016,117(4):417-422.
[10]TAKAHASHI K.Mannose-binding lectin and the balance between immune protection and complication[J].Expert Rev Anti Infect Ther,2011,9(12):1179-1190.
[11]NEGI V S,DEVARAJU P,MISRA D P,et al.Mannosebinding lectin(MBL)codon 54(rs1800450)polymorphism predisposes towards medium vessel vasculitis in patients with systemic lupus erythematosus[J].Clin Rheumatol,2017,36(4):837-843.
[12]WANG M,CHEN Y,ZHANG Y,et al.Mannan-binding lectin directly interacts with Toll-like receptor 4and suppresses lipopolysaccharide-induced inflammatory cytokine secretion from THP-1cells[J].Cell Mol Immunol,2011,8(3):265-275.
[13]SORBELLO V,CIPRANDI G,STEFANO A D,et al.Nasal IL-17Fis related to bronchial IL-17F/neutrophilia and exacerbations in stable atopic severe asthma[J].Allergy,2015,70(2):236-240.
[14]张印,崔艺馨,杨明会.呼吸道变应性炎症相关Th细胞因子的研究进展[J].中国医药导报,2016,13(16):31-34.
[15]WHITEHEAD G S,WILSON R H,NAKANO K,et al.IL-35Production by ICOS+Tregs Reverses Established IL-17-dependent Allergic Airways Disease[J].J Allergy Clin Immun,2012,129(1):207.