摘要
TSHR是一个调控鸟类季节性繁殖、影响产蛋性能的基因。研究旨在分析TSHR表达量及错义突变Gly558Arg与略阳乌鸡产蛋性能的关系。试验选取同一批孵化、相同环境饲养的116只略阳乌鸡、40只罗曼和15只尼克鸡,检测Gly558Arg分布情况。略阳乌鸡产蛋数(60±19)显著(P<0.05)低于罗曼(124±12)和尼克(119±7),且表现出更大的群体变异性。Gly558Arg在罗曼和尼克鸡中均被固定为优势基因型AA,但在略阳乌鸡中仍处于分离状态;劣势基因型AG以低频(4/116)存在。TSHR在高产略阳乌鸡下丘脑表达量显著(P<0.05)低于低产略阳乌鸡。本研究发现功能突变Gly558Arg在略阳乌鸡中尚未固定,在今后的育种工作中应该采用分子手段淘汰劣势基因型。除Gly558Arg外,TSHR表达差异可能也参与略阳乌鸡产蛋性能调控。
TSHR is a gene involving in regulation of seasonal reproduction and influencing egg production performance in bird. The aim of this study was to illuminate relationship of expression of TSHR and a nonsynonymous mutation of Gly558 Arg with egg production performance of Lueyang chickens. A total of 116 Lueyang, 40 Lohmann and 15 Nick chickens which hatched at the same time and had been raised in the same henhouse were used to detect distribution of Gly558 Arg. Egg number(60±19) of Lueyang chickens was significantly(P<0.05) lower than that of Lohmann(124±12)and Nick(119±7), and showed more variability.Gly558 Arg has been fixed as a superior genotype of AA in the Lohmann and Nick. But the nonsynonymous mutation keeps on separating in the Lueyang; inferior genotype of AG exists in extremely low(4/116) frequency. Expression level of TSHR in the hypothalamuses of high-production chickens was significantly(P<0.05) lower than that in low-production chickens. The data indicated that the functional mutation of Gly558 Arg has not been fixed in the Lueyang chicken yet, and suggested that the inferior genotype should be swept out using some molecular methods in the future breeding. Besides Gly558 Arg, differential expression of TSHR might be involved in regulation of egg production of Lueyang chickens.
引文
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