Dietary organic acid and fiber sources affect performance,intestinal morphology, immune responses and gut microflora in broilers
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摘要
This experiment was designed to investigate the effects of a dietary organic acid(OA) mixture and 2 fiber sources on performance, intestinal morphology, immune responses and gut microflora in broilers. A total of 390 one-day-old broiler chicks(Ross 308) were allocated to 6 dietary treatments with 5 replicate pens and 13 chicks each based on a factorial arrangement(2 x 3) in a completely randomized design. The experiment lasted 42 d. The following experimental diets and as well as their interaction were considered: a basal diet supplemented with or without OA(0 or 1 g/kg) and 2 fiber sources(sugar beet pulp[soluble fiber] or rice hull [insoluble fiber]; 0 or 30 g/kg). Dietary supplementation of OA increased daily weight gains of broilers across the entire rearing period(P < 0.05). The dietary fibrous materials did not affect the performance of broilers. Antibody titer against influenza disease virus was higher in birds fed diets containing rice hull compared with other experimental groups(P < 0.05). The population of Lactobacillus bacteria was greater in birds fed OA-added diets without or with 30 g/kg rice hull supplementation compared with other experimental groups(P < 0.05). In conclusion, dietary supplemental OA improved performance of broilers, and dietary supplemental OA with rice hull enhanced humoral immune responses.
This experiment was designed to investigate the effects of a dietary organic acid(OA) mixture and 2 fiber sources on performance, intestinal morphology, immune responses and gut microflora in broilers. A total of 390 one-day-old broiler chicks(Ross 308) were allocated to 6 dietary treatments with 5 replicate pens and 13 chicks each based on a factorial arrangement(2 x 3) in a completely randomized design. The experiment lasted 42 d. The following experimental diets and as well as their interaction were considered: a basal diet supplemented with or without OA(0 or 1 g/kg) and 2 fiber sources(sugar beet pulp[soluble fiber] or rice hull [insoluble fiber]; 0 or 30 g/kg). Dietary supplementation of OA increased daily weight gains of broilers across the entire rearing period(P < 0.05). The dietary fibrous materials did not affect the performance of broilers. Antibody titer against influenza disease virus was higher in birds fed diets containing rice hull compared with other experimental groups(P < 0.05). The population of Lactobacillus bacteria was greater in birds fed OA-added diets without or with 30 g/kg rice hull supplementation compared with other experimental groups(P < 0.05). In conclusion, dietary supplemental OA improved performance of broilers, and dietary supplemental OA with rice hull enhanced humoral immune responses.
This experiment was designed to investigate the effects of a dietary organic acid(OA) mixture and 2 fiber sources on performance, intestinal morphology, immune responses and gut microflora in broilers. A total of 390 one-day-old broiler chicks(Ross 308) were allocated to 6 dietary treatments with 5 replicate pens and 13 chicks each based on a factorial arrangement(2 x 3) in a completely randomized design. The experiment lasted 42 d. The following experimental diets and as well as their interaction were considered: a basal diet supplemented with or without OA(0 or 1 g/kg) and 2 fiber sources(sugar beet pulp[soluble fiber] or rice hull [insoluble fiber]; 0 or 30 g/kg). Dietary supplementation of OA increased daily weight gains of broilers across the entire rearing period(P < 0.05). The dietary fibrous materials did not affect the performance of broilers. Antibody titer against influenza disease virus was higher in birds fed diets containing rice hull compared with other experimental groups(P < 0.05). The population of Lactobacillus bacteria was greater in birds fed OA-added diets without or with 30 g/kg rice hull supplementation compared with other experimental groups(P < 0.05). In conclusion, dietary supplemental OA improved performance of broilers, and dietary supplemental OA with rice hull enhanced humoral immune responses.
引文
Abazari A, Navidshad B, Mirzaei Aghjehgheshlagh F, Nikbin S. The effect of rice husk as an insoluble dietary fiber source on intestinal morphology and Lactobacilli and Escherichia coli populations in broilers. Iran J Vet Med 2016;10:217-24.
Abdel-Fattah SA, El-Sanhoury MH, El-Mednay NM, Abdel-Azeem F. Thyroid activity,some blood constituents, organs morphology and performance of broiler chicks fed supplemental organic acids. Int J Poultry Sci 2008;7:215-22.
Adibmoradi M, Navidshad B, Faseleh Jahromi M. The effect of moderate levels of finely ground insoluble fibre on small intestine morphology, nutrient digestibility and performance of broiler chickens. Ital J Anim Sci 2016;15:310-7.
Adil S, Banday T, Bhat GA, Mir MS, Rehman M. Effect of dietary supplementation of organic acids on performance, intestinal histomorphology, and serum biochemistry of broiler chicken. Vet Med Int 2010;2010:1-7.
Alp M, KocabaGLi N, Kahraman R, Bostan K. Effects of dietary supplementation with organic acids and zinc bacitracin on ileal microflora, pH and performance in broilers. Turk J Vet Anim Sci 1999;23:451-6.
Ao T, Cantor AH, Pescatore AJ, Ford MJ, Pierce JL, Dawson KA. Effect of enzyme supplementation and acidification of diets on nutrient digestibility and growth performance of broiler chicks. Poultry Sci 2009;88:111-7.
AOAC Official methods of analysis. Washington DC:Association of Official Analytical Chemist; 2000.
Aviagen. Ross 308 broiler:nutrition specification. Newbridge, Midlothian, Scotland,UK:Ross Breeders Limited; 2014.
Biggs P, Parsons CM. The effects of several organic acids on growth performance,nutrient digestibilities, and cecal microbial populations in young chicks. Poultry Sci 2008;87:2581-9.
Corfield AP, Carroll D, Myerscough N, Probert CS. Mucins in the gastrointestinal tract in health and disease. Front Biosci 2001;6:D1321-57.
Debon SJJ, Tester RF. In vitro binding of calcium, iron and zinc by non-starch polysaccharides. Food Chem 2001;73:401-10.
Dehghani-Tafti N, Jahanian R. Effect of supplemental organic acids on performance,carcass characteristics, and serum biochemical metabolites in broilers fed diets containing different crude protein levels. Anim Feed Sci Technol 2016;211:109-16.
Emami NK, Naeini SZ, Ruiz-Feria CA. Growth performance, digestibility, immune response and intestinal morphology of male broilers fed phosphorus deficient diets supplemented with microbial phytase and organic acids. Livest Sci2013;157:506-13.
Ferket PR, Gernat AG. Factors that affect feed intake of meat birds:a review. Int J Poultry Sci 2006;5:905-11.
Fushimi T, Tayama K, Fukaya M, Kitakoshi K, Nakai N, Tsukamoto Y, Sato Y. Acetic acid feeding enhances glycogen repletion in liver and skeletal muscle of rats.J Nutr 2001;131:1973-7.
Gonzalez-Alvarado JM, Jimenez-Moreno E, Gonzalez-Sanchez D, Lazaro R,Mateos GG. Effect of inclusion of oat hulls and sugar beet pulp in the diet on productive performance and digestive traits of broilers from 1 to 42 days of age.Anim Feed Sci Technol 2010; 162:37-46.
Gonzalez-Alvarado JM, Jimenez-Moreno E, Lazaro R, Mateos GG. Effect of type of cereal, heat processing of the cereal, and inclusion of fiber in the diet on productive performance and digestive traits of broilers. Poultry Sci 2007;86:1705-15.
Gunal M, Yayli G, Kaya O, Karahan N, Sulak O. The effects of antibiotic growth promoter, probiotic or organic acid supplementation on performance, intestinal microflora and tissue of broilers. Int J Poultry Sci 2006;5:149-55.
Houshmand M, Azhar K, Zulkifli I, Bejo MH, Kamyab A. Effects of non-antibiotic feed additives on performance, immunity and intestinal morphology of broilers fed different levels of protein. S Afr J Anim Sci 2012;42:22-32.
Iji PA, Tivey DR. Natural and synthetic oligosaccharides in broiler chicken diets.World's Poult Sci J 1998;54:129-43.
Janssen W, Carre B. Influence of fibre on digestibility of poultry feeds. In:Haresign W, Cole DJA, editors. Recent advances in animal nutrition. London, UK:Butterworths; 1985.
Jimenez-Moreno E, de Coca-Sinova A, Gonzalez-Alvarado JM, Mateos GG. Inclusion of insoluble fiber sources in mash or pellet diets for young broilers. 1. Effects on growth performance and water intake. Poultry Sci 2016;95:41-52.
Jimenez-Moreno E, Frikha M, de Coca-Sinova A, Lazaro RP, Mateos GG. Oat hulls and sugar beet pulp in diets for broilers. 2. Effects on the development of the gastrointestinal tract and on the structure of the jejunal mucosa. Anim Feed Sci Technol 2013;182:44-52.
Jimenez-Moreno E, Gonzalez-Alvarado JM, Gonzalez-Sanchez D, Lázaro R,Mateos GG. Effects of type and particle size of dietary fiber on growth performance and digestive traits of broilers from 1 to 21 days of age 1. Poultry Sci2010;89:2197-212.
Jimenez-Moreno E, Gonzalez-Alvarado JM, Gonzalez-Serrano A, Lázaro R,Mateos GG. Effect of dietary fiber and fat on performance and digestive traits of broilers from one to twenty-one days of age. Poultry Sci 2009;88:2562-74.
Kalmendal R, Elwinger K, Holm L, Tauson R, High-fibre sunflower cake affects small intestinal digestion and health in broiler chickens. Br Poultry Sci 2011;52:86-96.Khan SH, Iqbal J. Recent advances in the role of organic acids in poultry nutrition.J Appl Anim Res 2016;44:359-69.
Mateos GG, Jimenez-Moreno E, Serrano MP, Lazaro RP. Poultry response to high levels of dietary fiber sources varying in physical and chemical characteristics.J Appl Poultry Res 2012;21:156-74.
Montagne L, Piel C, Lalles JP. Effect of diet on mucin kinetics and composition:nutrition and health implications. Nutr Rev 2004;62:105-14.
Montagne L, Pluske JR, Hampson DJ. A review of interactions between dietary fibre and the intestinal mucosa, and their consequences on digestive health in young non-ruminant animals. Anim Feed Sci Technol 2003;108:95-117.
Panda AK, Rao SVR, Raju M, Sunder GS. Effect of butyric acid on performance,gastrointestinal tract health and carcass characteristics in broiler chickens.Asian Aust J Anim Sci 2009;22:1026-31.
Parmentier HK, Baelmans R, Savelkoul HFJ, Dorny P, Demey F, Berkvens D. Serum haemolytic complement activities in 11 different MHC(B)typed chicken lines.Vet Immunol Immunopathol 2004;100:25-32.
Ragaa NM, Korany RMS. Studying the effect of formic acid and potassium diformate on performance, immunity and gut health of broiler chickens. Anim Nutr2016;2:296-302.
Rezaei M, Karimi Torshizi MA, Rouzbehan Y. The influence of different levels of micronized insoluble fiber on broiler performance and litter moisture. Poultry Sci 2011;90:2008-12.
Rodriguez-Lecompte JC, Yitbarek A, Brady J, Sharif S, Cavanagh MD, Crow G,Guenter W, House JD, Camelo-Jaimes G. The effect of microbial-nutrient interaction on the immune system of young chicks after early probiotic and organic acid administration. J Anim Sci 2012;90:2246-54.
Sadeghi A, Toghyani M, Gheisari A. Effect of various fiber types and choice feeding of fiber on performance, gut development, humoral immunity, and fiber preference in broiler chicks. Poultry Sci 2015;94:2734-43.
Sklan D, Smirnov A, Plavnik I. The effect of dietary fibre on the small intestines and apparent digestion in the Turkey. Br Poultry Sci 2003;44:735-40.
Teirlynck E, Bjerrum L, Eeckhaut V, Huygebaert G, Pasmans F, Haesebrouck F,Dewulf J, Ducatelle R, Van Immerseel F. The cereal type in feed influences gut wall morphology and intestinal immune cell infiltration in broiler chickens. Br J Nutr 2009;102:1453-61.
Van der Wielen PWJJ, Biesterveld S, Lipman LJA, van Knapen F. Inhibition of a glucose-limited sequencing fed-batch culture of Salmonella enterica serovar Enteritidis by volatile fatty acids representative of the ceca of broiler chickens.Appl Environ Microbiol 2001;67:1979-82.
Van Soest PJV, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci1991;74:3583-97.
Wegmann TG, Smithies O. A simple hemagglutination system requiring small amounts of red cells and antibodies. Transfusion 1966;6:67-73.
Wils-Plotz EL, Dilger RN. Combined dietary effects of supplemental threonine and purified fiber on growth performance and intestinal health of young chicks.Poultry Sci 2013;92:726-34.
Zhang WH, Jiang Y, Zhu QF, Gao F, Dai SF, Chen J, Zhou GH. Sodium butyrate maintains growth performance by regulating the immune response in broiler chickens. Br Poultry Sci 2011;52:292-301.
Abdel-Fattah SA, El-Sanhoury MH, El-Mednay NM, Abdel-Azeem F. Thyroid activity,some blood constituents, organs morphology and performance of broiler chicks fed supplemental organic acids. Int J Poultry Sci 2008;7:215-22.
Adibmoradi M, Navidshad B, Faseleh Jahromi M. The effect of moderate levels of finely ground insoluble fibre on small intestine morphology, nutrient digestibility and performance of broiler chickens. Ital J Anim Sci 2016;15:310-7.
Adil S, Banday T, Bhat GA, Mir MS, Rehman M. Effect of dietary supplementation of organic acids on performance, intestinal histomorphology, and serum biochemistry of broiler chicken. Vet Med Int 2010;2010:1-7.
Alp M, KocabaGLi N, Kahraman R, Bostan K. Effects of dietary supplementation with organic acids and zinc bacitracin on ileal microflora, pH and performance in broilers. Turk J Vet Anim Sci 1999;23:451-6.
Ao T, Cantor AH, Pescatore AJ, Ford MJ, Pierce JL, Dawson KA. Effect of enzyme supplementation and acidification of diets on nutrient digestibility and growth performance of broiler chicks. Poultry Sci 2009;88:111-7.
AOAC Official methods of analysis. Washington DC:Association of Official Analytical Chemist; 2000.
Aviagen. Ross 308 broiler:nutrition specification. Newbridge, Midlothian, Scotland,UK:Ross Breeders Limited; 2014.
Biggs P, Parsons CM. The effects of several organic acids on growth performance,nutrient digestibilities, and cecal microbial populations in young chicks. Poultry Sci 2008;87:2581-9.
Corfield AP, Carroll D, Myerscough N, Probert CS. Mucins in the gastrointestinal tract in health and disease. Front Biosci 2001;6:D1321-57.
Debon SJJ, Tester RF. In vitro binding of calcium, iron and zinc by non-starch polysaccharides. Food Chem 2001;73:401-10.
Dehghani-Tafti N, Jahanian R. Effect of supplemental organic acids on performance,carcass characteristics, and serum biochemical metabolites in broilers fed diets containing different crude protein levels. Anim Feed Sci Technol 2016;211:109-16.
Emami NK, Naeini SZ, Ruiz-Feria CA. Growth performance, digestibility, immune response and intestinal morphology of male broilers fed phosphorus deficient diets supplemented with microbial phytase and organic acids. Livest Sci2013;157:506-13.
Ferket PR, Gernat AG. Factors that affect feed intake of meat birds:a review. Int J Poultry Sci 2006;5:905-11.
Fushimi T, Tayama K, Fukaya M, Kitakoshi K, Nakai N, Tsukamoto Y, Sato Y. Acetic acid feeding enhances glycogen repletion in liver and skeletal muscle of rats.J Nutr 2001;131:1973-7.
Gonzalez-Alvarado JM, Jimenez-Moreno E, Gonzalez-Sanchez D, Lazaro R,Mateos GG. Effect of inclusion of oat hulls and sugar beet pulp in the diet on productive performance and digestive traits of broilers from 1 to 42 days of age.Anim Feed Sci Technol 2010; 162:37-46.
Gonzalez-Alvarado JM, Jimenez-Moreno E, Lazaro R, Mateos GG. Effect of type of cereal, heat processing of the cereal, and inclusion of fiber in the diet on productive performance and digestive traits of broilers. Poultry Sci 2007;86:1705-15.
Gunal M, Yayli G, Kaya O, Karahan N, Sulak O. The effects of antibiotic growth promoter, probiotic or organic acid supplementation on performance, intestinal microflora and tissue of broilers. Int J Poultry Sci 2006;5:149-55.
Houshmand M, Azhar K, Zulkifli I, Bejo MH, Kamyab A. Effects of non-antibiotic feed additives on performance, immunity and intestinal morphology of broilers fed different levels of protein. S Afr J Anim Sci 2012;42:22-32.
Iji PA, Tivey DR. Natural and synthetic oligosaccharides in broiler chicken diets.World's Poult Sci J 1998;54:129-43.
Janssen W, Carre B. Influence of fibre on digestibility of poultry feeds. In:Haresign W, Cole DJA, editors. Recent advances in animal nutrition. London, UK:Butterworths; 1985.
Jimenez-Moreno E, de Coca-Sinova A, Gonzalez-Alvarado JM, Mateos GG. Inclusion of insoluble fiber sources in mash or pellet diets for young broilers. 1. Effects on growth performance and water intake. Poultry Sci 2016;95:41-52.
Jimenez-Moreno E, Frikha M, de Coca-Sinova A, Lazaro RP, Mateos GG. Oat hulls and sugar beet pulp in diets for broilers. 2. Effects on the development of the gastrointestinal tract and on the structure of the jejunal mucosa. Anim Feed Sci Technol 2013;182:44-52.
Jimenez-Moreno E, Gonzalez-Alvarado JM, Gonzalez-Sanchez D, Lázaro R,Mateos GG. Effects of type and particle size of dietary fiber on growth performance and digestive traits of broilers from 1 to 21 days of age 1. Poultry Sci2010;89:2197-212.
Jimenez-Moreno E, Gonzalez-Alvarado JM, Gonzalez-Serrano A, Lázaro R,Mateos GG. Effect of dietary fiber and fat on performance and digestive traits of broilers from one to twenty-one days of age. Poultry Sci 2009;88:2562-74.
Kalmendal R, Elwinger K, Holm L, Tauson R, High-fibre sunflower cake affects small intestinal digestion and health in broiler chickens. Br Poultry Sci 2011;52:86-96.Khan SH, Iqbal J. Recent advances in the role of organic acids in poultry nutrition.J Appl Anim Res 2016;44:359-69.
Mateos GG, Jimenez-Moreno E, Serrano MP, Lazaro RP. Poultry response to high levels of dietary fiber sources varying in physical and chemical characteristics.J Appl Poultry Res 2012;21:156-74.
Montagne L, Piel C, Lalles JP. Effect of diet on mucin kinetics and composition:nutrition and health implications. Nutr Rev 2004;62:105-14.
Montagne L, Pluske JR, Hampson DJ. A review of interactions between dietary fibre and the intestinal mucosa, and their consequences on digestive health in young non-ruminant animals. Anim Feed Sci Technol 2003;108:95-117.
Panda AK, Rao SVR, Raju M, Sunder GS. Effect of butyric acid on performance,gastrointestinal tract health and carcass characteristics in broiler chickens.Asian Aust J Anim Sci 2009;22:1026-31.
Parmentier HK, Baelmans R, Savelkoul HFJ, Dorny P, Demey F, Berkvens D. Serum haemolytic complement activities in 11 different MHC(B)typed chicken lines.Vet Immunol Immunopathol 2004;100:25-32.
Ragaa NM, Korany RMS. Studying the effect of formic acid and potassium diformate on performance, immunity and gut health of broiler chickens. Anim Nutr2016;2:296-302.
Rezaei M, Karimi Torshizi MA, Rouzbehan Y. The influence of different levels of micronized insoluble fiber on broiler performance and litter moisture. Poultry Sci 2011;90:2008-12.
Rodriguez-Lecompte JC, Yitbarek A, Brady J, Sharif S, Cavanagh MD, Crow G,Guenter W, House JD, Camelo-Jaimes G. The effect of microbial-nutrient interaction on the immune system of young chicks after early probiotic and organic acid administration. J Anim Sci 2012;90:2246-54.
Sadeghi A, Toghyani M, Gheisari A. Effect of various fiber types and choice feeding of fiber on performance, gut development, humoral immunity, and fiber preference in broiler chicks. Poultry Sci 2015;94:2734-43.
Sklan D, Smirnov A, Plavnik I. The effect of dietary fibre on the small intestines and apparent digestion in the Turkey. Br Poultry Sci 2003;44:735-40.
Teirlynck E, Bjerrum L, Eeckhaut V, Huygebaert G, Pasmans F, Haesebrouck F,Dewulf J, Ducatelle R, Van Immerseel F. The cereal type in feed influences gut wall morphology and intestinal immune cell infiltration in broiler chickens. Br J Nutr 2009;102:1453-61.
Van der Wielen PWJJ, Biesterveld S, Lipman LJA, van Knapen F. Inhibition of a glucose-limited sequencing fed-batch culture of Salmonella enterica serovar Enteritidis by volatile fatty acids representative of the ceca of broiler chickens.Appl Environ Microbiol 2001;67:1979-82.
Van Soest PJV, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci1991;74:3583-97.
Wegmann TG, Smithies O. A simple hemagglutination system requiring small amounts of red cells and antibodies. Transfusion 1966;6:67-73.
Wils-Plotz EL, Dilger RN. Combined dietary effects of supplemental threonine and purified fiber on growth performance and intestinal health of young chicks.Poultry Sci 2013;92:726-34.
Zhang WH, Jiang Y, Zhu QF, Gao F, Dai SF, Chen J, Zhou GH. Sodium butyrate maintains growth performance by regulating the immune response in broiler chickens. Br Poultry Sci 2011;52:292-301.