Studies on the Analgesic Activity and Acute Toxicities of Bidens alba(L.) DC
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摘要
[Objectives] This study was conducted to investigate the analgesic effects and acute toxicities of Bidens alba(L.) DC. [Methods] The alcohol extract of B. alba(L.) DC was extracted and separated with petroleum ether and chloroform successively. The acute toxicities of the two extracts on mice were measured, and then the analgesic effects were measured with writhing pain model induced by acetic acid. [Results] No mice died when the crude dosages of B. alba(L.) DC from petroleum ether extract and chloroform extract were 5016 and 5100 mg/kg, respectively. When the petroleum ether extract was 60.0 mg/kg, the percentage of twisted mice induced by acetic acid was 40%, the analgesic rate was 77.5%, and the time of the first writhing was(294.0±165.8) s; when the chloroform extract was 20.0 mg/kg, the percentage of twisted animals was 55.6%, the analgesic rate was 51.5%, and the time of the first writhing was(273.8 ±153.4) s; and when the chloroform extract was 4.0 mg/kg, the percentage of twisted animals was 40%, and the analgesic rate was 62.1%, and the time of the first writhing was(370.6±231.3) s. [Conclusions] The petroleum ether extracts and chloroform extracts of B. alba(L.) DC have good analgesic effects and no acute toxicities.
[Objectives] This study was conducted to investigate the analgesic effects and acute toxicities of Bidens alba(L.) DC. [Methods] The alcohol extract of B. alba(L.) DC was extracted and separated with petroleum ether and chloroform successively. The acute toxicities of the two extracts on mice were measured, and then the analgesic effects were measured with writhing pain model induced by acetic acid. [Results] No mice died when the crude dosages of B. alba(L.) DC from petroleum ether extract and chloroform extract were 5016 and 5100 mg/kg, respectively. When the petroleum ether extract was 60.0 mg/kg, the percentage of twisted mice induced by acetic acid was 40%, the analgesic rate was 77.5%, and the time of the first writhing was(294.0±165.8) s; when the chloroform extract was 20.0 mg/kg, the percentage of twisted animals was 55.6%, the analgesic rate was 51.5%, and the time of the first writhing was(273.8 ±153.4) s; and when the chloroform extract was 4.0 mg/kg, the percentage of twisted animals was 40%, and the analgesic rate was 62.1%, and the time of the first writhing was(370.6±231.3) s. [Conclusions] The petroleum ether extracts and chloroform extracts of B. alba(L.) DC have good analgesic effects and no acute toxicities.
[Objectives] This study was conducted to investigate the analgesic effects and acute toxicities of Bidens alba(L.) DC. [Methods] The alcohol extract of B. alba(L.) DC was extracted and separated with petroleum ether and chloroform successively. The acute toxicities of the two extracts on mice were measured, and then the analgesic effects were measured with writhing pain model induced by acetic acid. [Results] No mice died when the crude dosages of B. alba(L.) DC from petroleum ether extract and chloroform extract were 5016 and 5100 mg/kg, respectively. When the petroleum ether extract was 60.0 mg/kg, the percentage of twisted mice induced by acetic acid was 40%, the analgesic rate was 77.5%, and the time of the first writhing was(294.0±165.8) s; when the chloroform extract was 20.0 mg/kg, the percentage of twisted animals was 55.6%, the analgesic rate was 51.5%, and the time of the first writhing was(273.8 ±153.4) s; and when the chloroform extract was 4.0 mg/kg, the percentage of twisted animals was 40%, and the analgesic rate was 62.1%, and the time of the first writhing was(370.6±231.3) s. [Conclusions] The petroleum ether extracts and chloroform extracts of B. alba(L.) DC have good analgesic effects and no acute toxicities.
引文
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[5] WEI CQ,PAN YM,TANG SC,et al.Seed germination of Bidens alba L.and B.biternata L.under different light and temperature regimes[J].Journal of Weed Science,2013(4):1-4.
[6] LIU MC,WEI CQ,TANG SC,et al.Bionomics of two invasive weeds,and,and their native congeners grown under different nutrient levels[J].Journal of Biosafety,2012(1):32-40.
[7] YUAN MG,GAO QY,XU ZH,et al.Comparative study on anti-inflammatory activities of two extracts from Bidens alba(L.) DC[J].Heilongjiang Animal Science and Veterinary Medicine,2018(13):167-169.
[8] GAO QY,QIN YW,YUAN MG.Studies On the Anti-Inflammatory Activity of Three Extraction Processes of Bidens Alba(L.) DC[J].Medicinal Plant,2018(6):68-71.
[9] ONG PL,WENG BC,LU FJ,et al.The anticancer effect of protein-extract from Bidens alba in human colorectal carcinoma SW480 cells via the reactive oxidative species- and glutathione depletion-dependent apoptosis[J].Food Chem Toxicol,2008,46(5):1535-1547.
[10] LI Y.Identification and purification of allergens in Bidens alba (L.) DC.pollen and verification through a model of mouse asthma and rhinitis[D].Jinzhou:Liaoning Medical University,2015.
[11] ZAPATA-MORALES JR,ALONSO-CASTRO AJ,DOMINGUEZ F,et al.The antinociceptive effects of a standardized ethanol extract of the Bidens odorata Cav (Asteraceae) leaves are mediated by ATP-sensitive K+ channels[J].J Ethnopharmacol,2017,207:30-33.
[12] FOTSO AF,LONGO F,DJOMENI PD,et al.Analgesic and antiinflammatory activities of the ethyl acetate fraction of Bidens pilosa (Asteraceae)[J].Inflammopharmacology,2014,22(2):105-114.
[13] POZHARITSKAYA ON,SHIKOV AN,MAKAROVA MN,et al.Anti-inflammatory activity of a HPLC-fingerprinted aqueous infusion of aerial part of Bidens tripartita L[J].Phytomedicine,2010,17(6):463-468.
[14] LI SY,LI SZ,WANG XP.Effects of bidens injection on central nervous system[J].Henan Traditional Chinese Medicine,1984(3):44-45.
[2] ZHONG JD,ZHOU XX,LI XL,et al.Effects of different seeding depths on seedling emergence and growth of Bidens alba,Praxelis clematidea and Ageratum conyzoides[J].Chinese Journal of Tropical Agriculture,2017(1):1-6.
[3] ZHONG JD,ZHOU HB,LIU KD,et al.Comparative study on seed biological characteristics of three invasive compositae species[J].Journal of Weed Science,2016(2):7-11.
[4] ZOU CP,CHEN JF,SUN YB,et al.Effects on cadmium’s accumulation and migration characteristics from Bidens alba L.[J].South China Agriculture,2015(7):23-26.
[5] WEI CQ,PAN YM,TANG SC,et al.Seed germination of Bidens alba L.and B.biternata L.under different light and temperature regimes[J].Journal of Weed Science,2013(4):1-4.
[6] LIU MC,WEI CQ,TANG SC,et al.Bionomics of two invasive weeds,and,and their native congeners grown under different nutrient levels[J].Journal of Biosafety,2012(1):32-40.
[7] YUAN MG,GAO QY,XU ZH,et al.Comparative study on anti-inflammatory activities of two extracts from Bidens alba(L.) DC[J].Heilongjiang Animal Science and Veterinary Medicine,2018(13):167-169.
[8] GAO QY,QIN YW,YUAN MG.Studies On the Anti-Inflammatory Activity of Three Extraction Processes of Bidens Alba(L.) DC[J].Medicinal Plant,2018(6):68-71.
[9] ONG PL,WENG BC,LU FJ,et al.The anticancer effect of protein-extract from Bidens alba in human colorectal carcinoma SW480 cells via the reactive oxidative species- and glutathione depletion-dependent apoptosis[J].Food Chem Toxicol,2008,46(5):1535-1547.
[10] LI Y.Identification and purification of allergens in Bidens alba (L.) DC.pollen and verification through a model of mouse asthma and rhinitis[D].Jinzhou:Liaoning Medical University,2015.
[11] ZAPATA-MORALES JR,ALONSO-CASTRO AJ,DOMINGUEZ F,et al.The antinociceptive effects of a standardized ethanol extract of the Bidens odorata Cav (Asteraceae) leaves are mediated by ATP-sensitive K+ channels[J].J Ethnopharmacol,2017,207:30-33.
[12] FOTSO AF,LONGO F,DJOMENI PD,et al.Analgesic and antiinflammatory activities of the ethyl acetate fraction of Bidens pilosa (Asteraceae)[J].Inflammopharmacology,2014,22(2):105-114.
[13] POZHARITSKAYA ON,SHIKOV AN,MAKAROVA MN,et al.Anti-inflammatory activity of a HPLC-fingerprinted aqueous infusion of aerial part of Bidens tripartita L[J].Phytomedicine,2010,17(6):463-468.
[14] LI SY,LI SZ,WANG XP.Effects of bidens injection on central nervous system[J].Henan Traditional Chinese Medicine,1984(3):44-45.