大豆根系吸收亚硒酸盐的生理特性研究
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摘要
通过生理学和药理学方法,探讨了大豆根系吸收亚硒酸盐的生理特性。结果表明:大豆离体根吸收亚硒酸盐速率随pH升高而降低。pH3.0和8.0时,大豆吸收亚硒酸盐速率随硒含量增加呈比例升高,数据符合直线方程。pH5.0时,大豆吸收亚硒酸盐随硒含量增加而升高直至饱和,数据符合米氏动力学曲线方程。pH3.0时,DNP、NaF和4℃抑制亚硒酸盐吸收的程度分别为15%、19%和23%;pH5.0时,分别达到81%、80%和79%;pH8.0时,分别为16%、8%和11%。进一步研究表明,pH3.0时,HgCl2和AgNO3抑制亚硒酸盐吸收的程度分别为78%和79%;pH5.0时,磷饥饿显著促进亚硒酸盐吸收;pH8.0时,NPPB、9-CA、NPAA、TEACl、NA和DIDS分别抑制亚硒酸吸收的程度为13%、17%、22%、7%、18%和6%。表明大豆根系能通过水通道以被动方式吸收H2SeO3,通过磷转运蛋白以主动方式吸收HSeO-3。
Physiological characteristics of selenite uptake by soybean roots was investigated by means of physiological and pharmacological methods.The results showed that the rate of selenite uptake by soybean excised roots declined with an increase of pH.Concentration-dependent kinetics suggested that the rate of selenite uptake increased in proportion to the Se concentration in the absorption solution at pH3.0 and pH8.0.Linear equations were fitted to the data.The rate of selenite uptake increased and followed saturation kinetics with increasing Se concentrations in the absorption solution at pH5.0.The data fitted a Michaelis-Menten saturation curve.The results showed that DNP,NaF and a temperature of 4℃ repressed the rate of selenite uptake by 15%,19% and 23% at pH3.0,by up to 81%,80% and 79% at pH5.0,and by 16%,8% and 11% at pH8.0.Further study suggested that HgCl2 and AgNO3inhibited selenite uptake by 78% and 79% at pH3.0.Phosphorus starvation increased the rate of selenite uptake by soybean roots at pH5.0.NPPB,9-CA,NPAA,TEACl,NA and DIDS inhibited selenite uptake by 13%,17%,22%,7%,18% and 6% at pH8.0.It demonstrated that selenite was absorbed passively by soybean roots through aquaporins in the form of H2SeO3 at pH3.0,and was absorbed actively through phosphorus transporters in the form of HSeO-3at pH5.0.
Physiological characteristics of selenite uptake by soybean roots was investigated by means of physiological and pharmacological methods.The results showed that the rate of selenite uptake by soybean excised roots declined with an increase of pH.Concentration-dependent kinetics suggested that the rate of selenite uptake increased in proportion to the Se concentration in the absorption solution at pH3.0 and pH8.0.Linear equations were fitted to the data.The rate of selenite uptake increased and followed saturation kinetics with increasing Se concentrations in the absorption solution at pH5.0.The data fitted a Michaelis-Menten saturation curve.The results showed that DNP,NaF and a temperature of 4℃ repressed the rate of selenite uptake by 15%,19% and 23% at pH3.0,by up to 81%,80% and 79% at pH5.0,and by 16%,8% and 11% at pH8.0.Further study suggested that HgCl2 and AgNO3inhibited selenite uptake by 78% and 79% at pH3.0.Phosphorus starvation increased the rate of selenite uptake by soybean roots at pH5.0.NPPB,9-CA,NPAA,TEACl,NA and DIDS inhibited selenite uptake by 13%,17%,22%,7%,18% and 6% at pH8.0.It demonstrated that selenite was absorbed passively by soybean roots through aquaporins in the form of H2SeO3 at pH3.0,and was absorbed actively through phosphorus transporters in the form of HSeO-3at pH5.0.
引文
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[9]向天勇,吴永尧,陈建英.大豆硒蛋白的生物学功能初探[J].营养学报,2004,26(6):460-463.(Xiang T Y,Wu Y Y,Chen J Y.A preliminary approach to biological function of soybean selenoprotein[J].Acta Nutrimenta Sinica,2004,26(6):460-463.)
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[13]张艳玲,潘根兴,李正文,等.土壤-植物系统中硒的迁移转化及低硒地区食物链中硒的调节[J].土壤与环境,2002,11(4):388-391.(Zhang Y L,Pan G X,Li Z W,et al.Translation of selenium in the system of soil-plant and it’s regulation in food-chain[J].Soil and Environmental Sciences,2002,11(4):388-391.)
[14]Li H F,Steve P M,Zhao F J.Selenium uptake,translocation and speciation in wheat supplied with selenate or selenite[J].New Phytologist,2008,178:92-102.
[15]Milne J.Haloselenate(IV)formation and selenous acid dissociation equilibria in hydrochloric and hydrofluoric acids[J].Canadian Journal of Chemistry,1987,65:316-321.
[16]Zhang L H,Shi W M,Wang X C.Difference in selenite absorption between high-and low-selenium rice cultivars and its mechanism[J].Plant and Soil,2006,282(1-2):183-193.
[17]Zhao X Q,Mitani N,Yamaji N,et al.Involvement of silicon influx transporter OsNIP2;1 in selenite uptake in rice[J].Plant Physiology,2010,153(4):1871-1877.
[18]Zhang L H,Hu B,Li W,et al.OsPT2,a phosphate transporter,is involved in active uptake of selenite in rice[J].New Phytologist,2014,201:1183-1191.
[19]Zhang Y L,Pan G X,Chen J,et al.Uptake and transport of selenite and selenate by soybean seedlings of two genotypes[J].Plant and Soil,2003,253:437-443.
[20]Bari R,Datt Pant B D,Stitt M,et al.PHO2,microRNA399,and PHR1 define a phosphate-signaling pathway in plants[J].Plant Physiology,2006,141(3):988-999.
[21]Liu F,Wang Z Y,Ren H Y,et al.OsSPX1 suppresses the function of OsPHR2 in the regulation of expression of OsPT2 and phosphate homeostasis in shoots of rice[J].The Plant Journal,2010,62:508-517.
[22]Ai P H,Sun S B,Zhao J N,et al.Two rice phosphate transporters,OsPht1;2 and OsPht1;6,have different functions and kinetic properties in uptake and translocation[J].The Plant Journal,2009,57:798-809.
[23]Jia H F,Ren H Y,Gu M,et al.Phosphate transporter gene,OsPht1;8,is involved in phosphate homeostasis in rice[J].Plant Physiology,2011,156:1164-1175.
[24]Sun S,Gu M,Cao Y,et al.A constitutive expressed phosphate transporter,OsPht1;1,modulates phosphate uptake and translocation in phosphate-replete rice[J].Plant Physiology,2012,159(4):1571-1581.
[25]Wu Z Y,Zhao J M,Gao R F,et al.Molecular cloning,characterization and expression analysis of two members of the pht1 family of phosphate transporters in Glycine max[J].PLoS One,2011,6(6):19752.
[26]Qin L,Zhao J,Tian J,et al.The high-affinity phosphate transporter GmPT5 regulates phosphate transport to nodules and nodulation in soybean[J].Plant Physiology,2012,159:1634-1643.
[2]Stadtman T C.Selenium-dependent enzymes[J].Annual Reviews Biochemistry,1980,49:93-110.
[3]Behne D,Weiler H,Kyriakopoulos A.Effects of selenium deficiency on testicular morphology and functionin rats[J].Journal of Reproduction and Fertility,1996,106:291-297.
[4]Taylor E W,Nadimpalli R G,Ramanathan C S.Genomic structures of viral agents in relation to the biosynthesis of selenoproteins[J].Biological Trace Element Research,1997,56:63-91.
[5]McKenzie R C,Rafferty T S,Beckett G J,et al.Effects of selenium on immunity and aging[M]//Hatfield D L,Marla J B,Gladyshev V N.Selenium:its molecular biology and role in human health.Norwell,Mass:Kluwer Academic Publishers,2001.
[6]赵中秋,郑海雷,张春光,等.土壤硒及其与植物硒营养的关系[J].生态学杂志,2003,22(1):22-25.(Zhao Z Q,Zheng H L,Zhang C G.Advances in the studies on selenium in soil and selenium biological effect[J].Chinese Journal of Ecology,2003,22(1):22-25.)
[7]刘铮.中国土壤微量元素[M].南京:江苏科学技术出版社,1996.(Liu Z.Microelements in soils of China[M].Nanjing:Jiangsu Science and Technology Publishing House,1996.)
[8]郑建仙,李璇.硒的天然有机化及在谷物食品中应用的研究[J].食品工业,1997(3):25-27.(Zheng J X,Li X.Studies on the natural organizing of selenium and its application in cereal foods[J].Cereal and Feed Industry,1997(3):25-27.)
[9]向天勇,吴永尧,陈建英.大豆硒蛋白的生物学功能初探[J].营养学报,2004,26(6):460-463.(Xiang T Y,Wu Y Y,Chen J Y.A preliminary approach to biological function of soybean selenoprotein[J].Acta Nutrimenta Sinica,2004,26(6):460-463.)
[10]Geering H R,Cary E E,Jones L H.Solubility and redox criteria for the possible forms of selenium in soils[J].Soil Science Society of America Proceedings,1968,32:35-40.
[11]布和敖斯尔,张东威,刘力.土壤硒区域环境分异及安全阈值的研究[J].土壤学报,1995,32(2):186-193.(Aosier B H,Zhang D W,Liu L.Regional environmental differentiation and regional safety threshold of soil selenium[J].Acta Pedologica Sinica,1995,32(2):186-193.)
[12]张东威,布和敖斯尔.确定不同环境下土壤硒的活性稳定性及安全阈值[J].水土保持研究,1994,1(5):82-87.(Zhang D W,Aosier B H.The determination of activity and stability as well as the safe threshold value of selenium in the soil under various circumstances[J].Research of Soil and Water Conservation,1994,1(5):82-87.)
[13]张艳玲,潘根兴,李正文,等.土壤-植物系统中硒的迁移转化及低硒地区食物链中硒的调节[J].土壤与环境,2002,11(4):388-391.(Zhang Y L,Pan G X,Li Z W,et al.Translation of selenium in the system of soil-plant and it’s regulation in food-chain[J].Soil and Environmental Sciences,2002,11(4):388-391.)
[14]Li H F,Steve P M,Zhao F J.Selenium uptake,translocation and speciation in wheat supplied with selenate or selenite[J].New Phytologist,2008,178:92-102.
[15]Milne J.Haloselenate(IV)formation and selenous acid dissociation equilibria in hydrochloric and hydrofluoric acids[J].Canadian Journal of Chemistry,1987,65:316-321.
[16]Zhang L H,Shi W M,Wang X C.Difference in selenite absorption between high-and low-selenium rice cultivars and its mechanism[J].Plant and Soil,2006,282(1-2):183-193.
[17]Zhao X Q,Mitani N,Yamaji N,et al.Involvement of silicon influx transporter OsNIP2;1 in selenite uptake in rice[J].Plant Physiology,2010,153(4):1871-1877.
[18]Zhang L H,Hu B,Li W,et al.OsPT2,a phosphate transporter,is involved in active uptake of selenite in rice[J].New Phytologist,2014,201:1183-1191.
[19]Zhang Y L,Pan G X,Chen J,et al.Uptake and transport of selenite and selenate by soybean seedlings of two genotypes[J].Plant and Soil,2003,253:437-443.
[20]Bari R,Datt Pant B D,Stitt M,et al.PHO2,microRNA399,and PHR1 define a phosphate-signaling pathway in plants[J].Plant Physiology,2006,141(3):988-999.
[21]Liu F,Wang Z Y,Ren H Y,et al.OsSPX1 suppresses the function of OsPHR2 in the regulation of expression of OsPT2 and phosphate homeostasis in shoots of rice[J].The Plant Journal,2010,62:508-517.
[22]Ai P H,Sun S B,Zhao J N,et al.Two rice phosphate transporters,OsPht1;2 and OsPht1;6,have different functions and kinetic properties in uptake and translocation[J].The Plant Journal,2009,57:798-809.
[23]Jia H F,Ren H Y,Gu M,et al.Phosphate transporter gene,OsPht1;8,is involved in phosphate homeostasis in rice[J].Plant Physiology,2011,156:1164-1175.
[24]Sun S,Gu M,Cao Y,et al.A constitutive expressed phosphate transporter,OsPht1;1,modulates phosphate uptake and translocation in phosphate-replete rice[J].Plant Physiology,2012,159(4):1571-1581.
[25]Wu Z Y,Zhao J M,Gao R F,et al.Molecular cloning,characterization and expression analysis of two members of the pht1 family of phosphate transporters in Glycine max[J].PLoS One,2011,6(6):19752.
[26]Qin L,Zhao J,Tian J,et al.The high-affinity phosphate transporter GmPT5 regulates phosphate transport to nodules and nodulation in soybean[J].Plant Physiology,2012,159:1634-1643.