益生菌与幽门螺杆菌对黏膜屏障的影响及其机制的研究进展
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摘要
消化系黏膜屏障对致病菌有防御功能,但是在根除幽门螺杆菌(H. pylori)过程中会导致黏膜屏障的破坏。大量研究表明,益生菌通过修复上皮细胞以及细胞间连接、减少黏液分泌和炎症反应以及缓解消化道菌群紊乱等方式修复黏膜屏障。本文主要综述幽门螺杆菌对消化系黏膜屏障的损害及其机制,以及益生菌对黏膜屏障损伤的保护及修复机制研究的进展。
The mucosal barrier of the digestive system has a protective function against pathogenic bacteria,but it may be damaged during the eradication of Helicobacter pylori. Numerous studies showed that probiotics repaired the mucosal barrier by repairing epithelial cells and intercellular connections,reducing mucus secretion and inflammation,and alleviating gastrointestinal flora disorders. In this paper,the damage of Helicobacter pylori to mucosal barrier and its mechanism were mainly outlined,as well as the protection and mechanism of probiotics against mucosal barrier injury.
The mucosal barrier of the digestive system has a protective function against pathogenic bacteria,but it may be damaged during the eradication of Helicobacter pylori. Numerous studies showed that probiotics repaired the mucosal barrier by repairing epithelial cells and intercellular connections,reducing mucus secretion and inflammation,and alleviating gastrointestinal flora disorders. In this paper,the damage of Helicobacter pylori to mucosal barrier and its mechanism were mainly outlined,as well as the protection and mechanism of probiotics against mucosal barrier injury.
引文
[1]KOCH C,DA SILVEIRA A B M,DE OLIVEIRA E C,et al.Epithelial cell types and their proposed roles in maintaining the mucosal barrier in human chagasic-megacolonic mucosa[J].Histochem Cell Biol,2017,148(2):207-216.
[2]CARON T J,SCOTT K E,FOX J G,et al.Tight junction disruption:Helicobacter pylori and dysregulation of the gastric mucosal barrier[J].World J Gastroenterol,2015,21(40):11411-11427.
[3]BORGDORFF H,GAUTAM R,ARMSTRONG S D,et al.Cervicovaginal microbiome dysbiosis is associated with proteome changes related to alterations of the cervicovaginal mucosal barrier[J].Mucosal Immunol,2016,9(3):621-633.
[4]KONG W,HUANG C,TANG Y,et al.Effect of Bacillus subtilis on Aeromonas hydrophila-induced intestinal mucosal barrier function damage and inflammation in grass carp(Ctenopharyngodon idella)[J].Sci Rep,2017,7(1):1588.
[5]MITCHELL E L,DAVIS A T,BRASS K,et al.Reduced intestinal motility,mucosal barrier function,and inflammation in aged monkeys[J].J Nutr Healthaging,2017,21(4):354-361.
[6]CHEN E B,CASON C,GILBERT J A,et al.Current state of knowledge on implications of gut microbiome for surgical conditions[J].J Gastrointest Surg,2018,22(6):1112-1123.
[7]HEINTZ-BUSCHART A,WILMES P.Human Gut Microbiome:Function Matters[J].Trends Microbiol,2018,26(7):563-574.
[8]DUNN B E,COHEN H,BLASER M J.Helicobacter pylori[J].Clin Microbiol Rev,1997,10(4):720.
[9]ODENBREIT S,PLS J,SEDLMAIER B,et al.Translocation of Helicobacter pylori CagA into gastric epithelial cells by type IVsecretion[J].Science,2000,287(5457):1497-1500.
[10]MORELLI L,CAPURSO L.FAO/WHO guidelines on probiotics10 Years Later[J].J Clin Gastroenterol,2012,46:S1-S2.
[11]北京地区幽门螺杆菌多中心临床试验协作组.不同组合的三联疗法对幽门螺杆菌阳性十二指肠溃疡的疗效及一年随访研究[J].中华医学杂志,2004,84(14):21-25.
[12]MALFERTHEINER P,MEGRAUD F,O'MORAIN C A,et al.Management of Helicobacter pylori infection-the Maastricht V/Florence consensus report[J].Gut,2017,66(1):6-30.
[13]ZHANG Y,LI S,GAN R,et al.Impacts of Gut Bacteria on Human Health and Diseases[J].Int J Mol Sci,2015,16(4):7493-7519.
[14]SZAJEWSKA H,ALBRECHT P,TOPCZEWSKA-CABANEKA.Randomized,double-blind,placebo-controlled trial:effect of lactobacillus GG supplementation on Helicobacter pylori eradication rates and side effects during treatment in children[J].J Pediatr Gastroenterol Nutr,2009,48(4):431-436.
[15]于婧,夏永军,王光强,等.益生菌及益生元调节骨代谢的研究进展[J].工业微生物,2016,46(3):56-62.
[16]ZHANG S,GUO J,LIU L.Treating bacteria with bacteria:the role of probiotics in the eradication of Helicobacter pylori[J].Int JClin Exp Med,2017,10(3):4330-4341.
[17]YANG Y,DU J,LIU F,et al.Role of caspase-3/E-cadherin in helicobacter pylori-induced apoptosis of gastric epithelial cellsc[J].Oncotarget,2017,8(35):59204-59216.
[18]MNICH E,KOWALEWICZ-KULBAT M,SICI?SKA P,et al.[J].World J Gastroenterol,2016,22(33):7536-7558.
[19]YAN M,WANG H,SUN J,et al.Cutting edge:expression of IRF8 in gastric epithelial cells confers protective innate immunity against Helicobacter pylori infection[J].J Immunol,2016,196(5):1999-2003.
[20]KEILBERG D,OTTEMANN K M.How Helicobacter pylori senses,targets and interacts with the gastric epithelium[J].Environ Microbiol,2016,18(3):791-806.
[21]WU J,LIN K,ZENG J,et al.Role of DC-SIGN in Helicobacter pylori infection of gastrointestinal epithelial cells[J].Front Biosci(Landmark Ed),2014,19:825-834.
[22]MOYAT M,VELIN D.Immune responses to Helicobacter pylori infection[J].World J Gastroenterol,2014,20(19):5583-5593.
[23]KIVRAK SALIM D,SAHIN M,KKSOY S,et al.Local immune response in Helicobacter pylori infection[J].Medicine(Baltimore),2016,95(20):e3713.
[24]LI T,SHAO W,LI S,et al.H.pylori infection induced BMAL1 expression and rhythm disorder aggravate gastric inflammation[J].EBIOMEDICINE,2019,39:301-314.
[25]TRAN L S,TRAN D,DE PAOLI A,et al.NOD1 is required for Helicobacter pylori induction of IL-33 responses in gastric epithelial cells[J].Cell Microbiol,2018,20(5):e12826.
[26]RAMIS I B,VIANNA J S,GONCALVES C V,et al.Polymorphisms of the IL-6,IL-8 and IL-10 genes and the risk of gastric pathology in patients infected with Helicobacter pylori[J].J Microbiol Immunol Infect,2017,50(2):153-159.
[27]ZHAO Y,ZHOU Y,LIN G,et al.Association between IL-17,IL-8 and IL-18 expression in peripheral blood and helicobacter pylori infection in mongolian gerbils[J].Jundishapur J Microbiol,2015,8(8):e21503.
[28]CAO L,YU J.Effect of Helicobacter pylori infection on the composition of gastric microbiota in the development of gastric cancer[J].Gastrointest Tumors,2015,2(1):14-25.
[29]ALARCóN T,LLORCA L,PEREZ-PEREZ G.Impact of the microbiota and gastric disease development by Helicobacter pylori[J].Curr Top Microbiol Immunol,2017,400:253-275.
[30]BRAWNER K M,KUMAR R,SERRANO C A,et al.Helicobacter pylori infection is associated with an altered gastric microbiota in children[J].Mucosal Immunol,2017,10(5):1169-1177.
[31]KHUSNUTDINOVA D R,MARKELOVA M I,BOULYGINA EA,et al.The Effect of Helicobacter pylori Eradication on Human Microbiota:Metagenome Analysis of the Human Gut Microbiome[J].Uchenye Zapiski Kazanskogo Universiteta.Seriya Eatestvennye Nauki,2017,159(2):217-231
[32]REN X,ZHU Y,GAMALLAT Y,et al.E.coli O124 K72 alters the intestinal barrier and the tight junctions proteins of guinea pig intestine[J].Biomed Pharmacother,2017,94:468-473.
[33]WU Y,ZHU C,CHEN Z,et al.Protective effects of Lactobacillus plantarum on epithelial barrier disruption caused by enterotoxigenic Escherichia coli in intestinal porcine epithelial cells[J].Vet Immunol Immunopathol,2016,172:55-63.
[34]YU Q,YUAN L,DENG J,et al.Lactobacillus protects the integrity of intestinal epithelial barrier damaged by pathogenic bacteria[J].Front Cell Infect Microbiol,2015,5:26.
[35]GOMI A,HARIMA-MIZUSAWA N,SHIBAHARA-SONE H,et al.Effect of Bifidobacterium bifidum BF-1 on gastric protection and mucin production in an acute gastric injury rat model[J].JDairy Sci,2013,96(2):832-837.
[36]CELLI J P,TURNER B S,AFDHAL N H,et al.Helicobacter pylori moves through mucus by reducing mucin viscoelasticity[J].Proc Natl Acad Sci U S A,2009,106(34):14321-14326.
[37]NIV Y.Helicobacter pylori and gastric mucin expression:A systematic review and meta-analysis[J].World J Gastroenterol,2015,21(31):9430-9436.
[38]MACK D R,AHRNE S,HYDE L,et al.Extracellular MUC3mucin secretion follows adherence of Lactobacillus strains to intestinal epithelial cells in vitro[J].GUT,2003,52(6):827-833.
[39]AIBA Y,SUZUKI N,KABIR A M,et al.Lactic acid-mediated suppression of Helicobacter pylori by the oral administration of Lactobacillus salivarius as a probiotic in a gnotobiotic murine model[J].Am J Gastroenterol,1998,93(11):2097-2101.
[40]GARCIA-CASTILLO V,ZELAYA H,ILABACA A,et al.Lactobacillus fermentum UCO-979C beneficially modulates the innate immune response triggered by Helicobacter pylori infection in vitro[J].Benef Microbes,2018,9(5):829-841.
[41]SUNANLIGANON C,THONG-NGAM D,TUMWASORN S,et al.Lactobacillus plantarum B7 inhibits Helicobacter pylori growth and attenuates gastric inflammation[J].World J Gastroenterol,2012,18(20):2472-2480.
[42]BOLTIN D.Probiotics in Helicobacter pylori-induced peptic ulcer disease[J].Best Pract Res Clin Gastroenterol,2016,30(1):99-109.
[43]HOLZ C,BUSJAHN A,MEHLING H,et al.Significant reduction in Helicobacter pylori load in humans with non-viable Lactobacillus reuteri DSM17648:a pilot study[J].Probiotics Antimicrob Proteins,2015,7(2):91-100.
[44]OH B,KIM B S,KIM J W,et al.The effect of probiotics on gut microbiota during the Helicobacter pylori eradication:randomized controlled trial[J].Helicobacter,2016,21(3):165-174.
[45]宋岩,曹晓沧,杨永妍,等.益生菌修复幽门螺杆菌相关黏膜屏障破坏的机制[J].世界华人消化杂志,2013(28):2981-2986.
[2]CARON T J,SCOTT K E,FOX J G,et al.Tight junction disruption:Helicobacter pylori and dysregulation of the gastric mucosal barrier[J].World J Gastroenterol,2015,21(40):11411-11427.
[3]BORGDORFF H,GAUTAM R,ARMSTRONG S D,et al.Cervicovaginal microbiome dysbiosis is associated with proteome changes related to alterations of the cervicovaginal mucosal barrier[J].Mucosal Immunol,2016,9(3):621-633.
[4]KONG W,HUANG C,TANG Y,et al.Effect of Bacillus subtilis on Aeromonas hydrophila-induced intestinal mucosal barrier function damage and inflammation in grass carp(Ctenopharyngodon idella)[J].Sci Rep,2017,7(1):1588.
[5]MITCHELL E L,DAVIS A T,BRASS K,et al.Reduced intestinal motility,mucosal barrier function,and inflammation in aged monkeys[J].J Nutr Healthaging,2017,21(4):354-361.
[6]CHEN E B,CASON C,GILBERT J A,et al.Current state of knowledge on implications of gut microbiome for surgical conditions[J].J Gastrointest Surg,2018,22(6):1112-1123.
[7]HEINTZ-BUSCHART A,WILMES P.Human Gut Microbiome:Function Matters[J].Trends Microbiol,2018,26(7):563-574.
[8]DUNN B E,COHEN H,BLASER M J.Helicobacter pylori[J].Clin Microbiol Rev,1997,10(4):720.
[9]ODENBREIT S,PLS J,SEDLMAIER B,et al.Translocation of Helicobacter pylori CagA into gastric epithelial cells by type IVsecretion[J].Science,2000,287(5457):1497-1500.
[10]MORELLI L,CAPURSO L.FAO/WHO guidelines on probiotics10 Years Later[J].J Clin Gastroenterol,2012,46:S1-S2.
[11]北京地区幽门螺杆菌多中心临床试验协作组.不同组合的三联疗法对幽门螺杆菌阳性十二指肠溃疡的疗效及一年随访研究[J].中华医学杂志,2004,84(14):21-25.
[12]MALFERTHEINER P,MEGRAUD F,O'MORAIN C A,et al.Management of Helicobacter pylori infection-the Maastricht V/Florence consensus report[J].Gut,2017,66(1):6-30.
[13]ZHANG Y,LI S,GAN R,et al.Impacts of Gut Bacteria on Human Health and Diseases[J].Int J Mol Sci,2015,16(4):7493-7519.
[14]SZAJEWSKA H,ALBRECHT P,TOPCZEWSKA-CABANEKA.Randomized,double-blind,placebo-controlled trial:effect of lactobacillus GG supplementation on Helicobacter pylori eradication rates and side effects during treatment in children[J].J Pediatr Gastroenterol Nutr,2009,48(4):431-436.
[15]于婧,夏永军,王光强,等.益生菌及益生元调节骨代谢的研究进展[J].工业微生物,2016,46(3):56-62.
[16]ZHANG S,GUO J,LIU L.Treating bacteria with bacteria:the role of probiotics in the eradication of Helicobacter pylori[J].Int JClin Exp Med,2017,10(3):4330-4341.
[17]YANG Y,DU J,LIU F,et al.Role of caspase-3/E-cadherin in helicobacter pylori-induced apoptosis of gastric epithelial cellsc[J].Oncotarget,2017,8(35):59204-59216.
[18]MNICH E,KOWALEWICZ-KULBAT M,SICI?SKA P,et al.[J].World J Gastroenterol,2016,22(33):7536-7558.
[19]YAN M,WANG H,SUN J,et al.Cutting edge:expression of IRF8 in gastric epithelial cells confers protective innate immunity against Helicobacter pylori infection[J].J Immunol,2016,196(5):1999-2003.
[20]KEILBERG D,OTTEMANN K M.How Helicobacter pylori senses,targets and interacts with the gastric epithelium[J].Environ Microbiol,2016,18(3):791-806.
[21]WU J,LIN K,ZENG J,et al.Role of DC-SIGN in Helicobacter pylori infection of gastrointestinal epithelial cells[J].Front Biosci(Landmark Ed),2014,19:825-834.
[22]MOYAT M,VELIN D.Immune responses to Helicobacter pylori infection[J].World J Gastroenterol,2014,20(19):5583-5593.
[23]KIVRAK SALIM D,SAHIN M,KKSOY S,et al.Local immune response in Helicobacter pylori infection[J].Medicine(Baltimore),2016,95(20):e3713.
[24]LI T,SHAO W,LI S,et al.H.pylori infection induced BMAL1 expression and rhythm disorder aggravate gastric inflammation[J].EBIOMEDICINE,2019,39:301-314.
[25]TRAN L S,TRAN D,DE PAOLI A,et al.NOD1 is required for Helicobacter pylori induction of IL-33 responses in gastric epithelial cells[J].Cell Microbiol,2018,20(5):e12826.
[26]RAMIS I B,VIANNA J S,GONCALVES C V,et al.Polymorphisms of the IL-6,IL-8 and IL-10 genes and the risk of gastric pathology in patients infected with Helicobacter pylori[J].J Microbiol Immunol Infect,2017,50(2):153-159.
[27]ZHAO Y,ZHOU Y,LIN G,et al.Association between IL-17,IL-8 and IL-18 expression in peripheral blood and helicobacter pylori infection in mongolian gerbils[J].Jundishapur J Microbiol,2015,8(8):e21503.
[28]CAO L,YU J.Effect of Helicobacter pylori infection on the composition of gastric microbiota in the development of gastric cancer[J].Gastrointest Tumors,2015,2(1):14-25.
[29]ALARCóN T,LLORCA L,PEREZ-PEREZ G.Impact of the microbiota and gastric disease development by Helicobacter pylori[J].Curr Top Microbiol Immunol,2017,400:253-275.
[30]BRAWNER K M,KUMAR R,SERRANO C A,et al.Helicobacter pylori infection is associated with an altered gastric microbiota in children[J].Mucosal Immunol,2017,10(5):1169-1177.
[31]KHUSNUTDINOVA D R,MARKELOVA M I,BOULYGINA EA,et al.The Effect of Helicobacter pylori Eradication on Human Microbiota:Metagenome Analysis of the Human Gut Microbiome[J].Uchenye Zapiski Kazanskogo Universiteta.Seriya Eatestvennye Nauki,2017,159(2):217-231
[32]REN X,ZHU Y,GAMALLAT Y,et al.E.coli O124 K72 alters the intestinal barrier and the tight junctions proteins of guinea pig intestine[J].Biomed Pharmacother,2017,94:468-473.
[33]WU Y,ZHU C,CHEN Z,et al.Protective effects of Lactobacillus plantarum on epithelial barrier disruption caused by enterotoxigenic Escherichia coli in intestinal porcine epithelial cells[J].Vet Immunol Immunopathol,2016,172:55-63.
[34]YU Q,YUAN L,DENG J,et al.Lactobacillus protects the integrity of intestinal epithelial barrier damaged by pathogenic bacteria[J].Front Cell Infect Microbiol,2015,5:26.
[35]GOMI A,HARIMA-MIZUSAWA N,SHIBAHARA-SONE H,et al.Effect of Bifidobacterium bifidum BF-1 on gastric protection and mucin production in an acute gastric injury rat model[J].JDairy Sci,2013,96(2):832-837.
[36]CELLI J P,TURNER B S,AFDHAL N H,et al.Helicobacter pylori moves through mucus by reducing mucin viscoelasticity[J].Proc Natl Acad Sci U S A,2009,106(34):14321-14326.
[37]NIV Y.Helicobacter pylori and gastric mucin expression:A systematic review and meta-analysis[J].World J Gastroenterol,2015,21(31):9430-9436.
[38]MACK D R,AHRNE S,HYDE L,et al.Extracellular MUC3mucin secretion follows adherence of Lactobacillus strains to intestinal epithelial cells in vitro[J].GUT,2003,52(6):827-833.
[39]AIBA Y,SUZUKI N,KABIR A M,et al.Lactic acid-mediated suppression of Helicobacter pylori by the oral administration of Lactobacillus salivarius as a probiotic in a gnotobiotic murine model[J].Am J Gastroenterol,1998,93(11):2097-2101.
[40]GARCIA-CASTILLO V,ZELAYA H,ILABACA A,et al.Lactobacillus fermentum UCO-979C beneficially modulates the innate immune response triggered by Helicobacter pylori infection in vitro[J].Benef Microbes,2018,9(5):829-841.
[41]SUNANLIGANON C,THONG-NGAM D,TUMWASORN S,et al.Lactobacillus plantarum B7 inhibits Helicobacter pylori growth and attenuates gastric inflammation[J].World J Gastroenterol,2012,18(20):2472-2480.
[42]BOLTIN D.Probiotics in Helicobacter pylori-induced peptic ulcer disease[J].Best Pract Res Clin Gastroenterol,2016,30(1):99-109.
[43]HOLZ C,BUSJAHN A,MEHLING H,et al.Significant reduction in Helicobacter pylori load in humans with non-viable Lactobacillus reuteri DSM17648:a pilot study[J].Probiotics Antimicrob Proteins,2015,7(2):91-100.
[44]OH B,KIM B S,KIM J W,et al.The effect of probiotics on gut microbiota during the Helicobacter pylori eradication:randomized controlled trial[J].Helicobacter,2016,21(3):165-174.
[45]宋岩,曹晓沧,杨永妍,等.益生菌修复幽门螺杆菌相关黏膜屏障破坏的机制[J].世界华人消化杂志,2013(28):2981-2986.