血清miR-132和miR-320a对精神分裂症的诊断价值
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摘要
目的:探讨精神分裂症患者血清miR-132和miR-320a的相对表达水平及在精神分裂诊断中的应用价值。方法:收集80例首发精神分裂患者和80例健康体检者血清为研究对象,采用实时荧光定量PCR法(RT-PCR)检测血清中miR-132和miR-320a的表达水平,绘制受试者工作特征曲线(ROC),评价其对精神分裂症的诊断价值。结果:精神分裂症患者血清中miR-132、miR-320a表达水平分别为0.64±0.44、0.45±0.38,均显著低于健康体检者的1.62±1.26、1.40±1.03,差异均有统计学意义(P<0.05)。ROC曲线显示,miR-132和miR-320a诊断精神分裂症的AUC分别0.76[95%CI(0.68,0.83)]、0.81[95%CI(0.75,0.88)],敏感性分别为58.75%、68.4%,特异性分别为和71.25%、84%;两者联合检测预测值PRE的AUC为0.89[95%CI(0.84,0.94)],敏感性和特异性分别为78.92%和88.9%。结论:精神分裂症血清中miR-132和miR-320a显著下降,两者联合检测对精神分裂症有诊断参考意义。
Objective:To investigate the relative expression levels of serum miR-132 and miR-320 a in schizophrenic patients and their application in the diagnosis of schizophrenia.Method:The serum of 80 first-episode schizophrenic patients and 80 healthy volunteers were collected.The expression levels of miR-132 and miR-320 a in serum were detected by real-time fluorescent quantitative PCR(RT-PCR).Receiver operating characteristic(ROC) curve analysis was used to determine the diagnostic value of miR-132 and miR-320 a for schizophrenia.Result:The expression of miR-132,miR-320 a in serum of schizophrenic patients was(0.64±0.44),(0.45±0.38),which was significantly lower than(1.62±1.26),(1.40±1.03) of healthy controls.The difference was statistically significant(P<0.05).The AUC for miR-132 was 0.76[95%CI(0.68,0.83)],sensitivity and specificity were 58.75% and 71.25%,respectively.The AUC for miR-320 a was 0.81[95%CI(0.75,0.88)],sensitivity and specificity were 68.4% and 84%,respectively.The AUC of the combined detection PRE was 0.89[95%CI(0.84,0.94)],and the sensitivity and specificity were 78.92% and 88.9%,respectively.Conclusion:The serum levels of miR-132 and miR-320 a in schizophrenia are significantly decreased.The combined detection of miR-132 and miR-320 a has diagnostic significance for schizophrenia.
Objective:To investigate the relative expression levels of serum miR-132 and miR-320 a in schizophrenic patients and their application in the diagnosis of schizophrenia.Method:The serum of 80 first-episode schizophrenic patients and 80 healthy volunteers were collected.The expression levels of miR-132 and miR-320 a in serum were detected by real-time fluorescent quantitative PCR(RT-PCR).Receiver operating characteristic(ROC) curve analysis was used to determine the diagnostic value of miR-132 and miR-320 a for schizophrenia.Result:The expression of miR-132,miR-320 a in serum of schizophrenic patients was(0.64±0.44),(0.45±0.38),which was significantly lower than(1.62±1.26),(1.40±1.03) of healthy controls.The difference was statistically significant(P<0.05).The AUC for miR-132 was 0.76[95%CI(0.68,0.83)],sensitivity and specificity were 58.75% and 71.25%,respectively.The AUC for miR-320 a was 0.81[95%CI(0.75,0.88)],sensitivity and specificity were 68.4% and 84%,respectively.The AUC of the combined detection PRE was 0.89[95%CI(0.84,0.94)],and the sensitivity and specificity were 78.92% and 88.9%,respectively.Conclusion:The serum levels of miR-132 and miR-320 a in schizophrenia are significantly decreased.The combined detection of miR-132 and miR-320 a has diagnostic significance for schizophrenia.
引文
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[17]黄远帅,周炜鑫,彭瑛,等.精神分裂症相关mi RNA筛选及mi R-320a调控ITGB1机制研究[J].中国细胞生物学学报,2016(3):285-291.
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[20]Guan F,Zhang B,Yan T,et al.MIR137 gene and target gene CACNA1C of miR-137 contribute to schizophrenia susceptibility in Han Chinese[J].Schizophr Res,2014,152(1):97.
[2]肖川,周敏,杨珉,等.我国精神分裂症患者家庭负担城乡差异的Meta分析[J].现代预防医学,2016,43(3):498-502.
[3]冯莎莎,罗波,殷明刚,等.血浆miR-31-5p和miR-134-5p对精神分裂症的诊断价值[J].临床检验杂志,2018,36(2):116-119.
[4]Jin X F,Wu N,Wang L,et al.Circulating MicroRNAs:A Novel Class of Potential Biomarkers for Diagnosing and Prognosing Central Nervous System Diseases[J].Cell mol Neurobiol,2013,33(5):601-613.
[5]Liu Y,Chang X,Hahn C G,et al.Non-coding RNA dysregulation in the amygdala region of schizophrenia patients contributes to the pathogenesis of the disease[J].Transl Psychiatry,2018,8(1):44-54.
[6]冯莎莎,王开正,杨梅.miRNA作为精神分裂症分子标志物的研究进展[J].临床检验杂志,2014,32(10):771-773.
[7]李晓松.医学统计学[M].第2版.北京:高等教育出版社,2008:205-235.
[8]Carter J V,Pan J,Rai S N,et al.ROC-ing along:Evaluation and interpretation of receiver operating characteristic curves[J].Surgery,2016,159(6):1638-1645.
[9]Pickard B S.Schizophrenia biomarkers:translating the descriptive into the diagnostic[J].J Psychopharmacol,2015,29(2):138-143.
[10]Hass J,Walton E,Wright C,et al.Associations between DNAmethylation and schizophrenia-related intermediate phenotypes-a gene set enrichment analysis.[J].Prog Neuro-psychoph,2015,59:31-39.
[11]Laursen T M,Nordentoft M,Mortensen P B.Excess Early Mortality in Schizophrenia[J].Annu Rev Clin Psychol,2014,10(10):425-448.
[12]Nowak J S,Michlewski G.miRNAs in development and pathogenesis of the nervous system.[J].Biochem Soc Trans,2013,41(4):815-820.
[13]Wang J,Wang Y,Yang J,et al.MicroRNAs as novel biomarkers of schizophrenia[J].Exp Ther Med,2014,8(6):1671-1676.
[14]Sun E,Shi Y.MicroRNAs:Small molecules with big roles in neurodevelopment and diseases[J].Exp Neurol,2015,268(6):46-53.
[15]Nguyen L S,Lepleux M,Makhlouf M,et al.Profiling olfactory stem cells from living patients identifies miRNAs relevant for autism pathophysiology[J].Mol Autism,2016,7(1):1-13.
[16]Yu H,Wu J,Zhang H,et al.Alterations of miR-132 are novel diagnostic biomarkers in peripheral blood of schizophrenia patients[J].Prog Neuropsychoph,2015,63:23-29.
[17]黄远帅,周炜鑫,彭瑛,等.精神分裂症相关mi RNA筛选及mi R-320a调控ITGB1机制研究[J].中国细胞生物学学报,2016(3):285-291.
[18]Vachev T I,Popov N T,Stoyanova V K,et al.Down regulation of MIR-320 gene family members in the peripheral blood of schizophrenia patients[J].Int J Curr Microbiol App Sci,2016,5:221-230.
[19]Ziats M N,Rennert O M.Identification of Differentially Expressed MicroRNAs Across the Developing Human Brain[J].Mol Psychiatr,2014,19(7):848-852.
[20]Guan F,Zhang B,Yan T,et al.MIR137 gene and target gene CACNA1C of miR-137 contribute to schizophrenia susceptibility in Han Chinese[J].Schizophr Res,2014,152(1):97.