摘要
利用分布于小麦全基因组的369对SSR分子标记,分析98份育种亲本材料的基因型,并采用TASSLE软件的GLM和MLM模型鉴定除草剂抗性紧密关联的标记位点、发掘关联标记的优异等位类型。以2,4-滴丁酯处理条件下的芽鲜重抑制率(T1)、甲基二磺隆处理下的芽鲜重抑制率(T2)和根鲜重抑制率(T3)等3个指标为目标性状,共关联到37个显著标记位点(P<0.05),分布于小麦染色体1AL、1BL、1DL、2AS、2BS、3AL、3BL、3BS、4AL、4BL、5AL、5DL、5DS、6BL、6DL、6DS、7AL、7BL、7BS、7DS和7DL上。其中3BS和4BL上的分子标记Xgwm285和Xgwm165同时在3个指标中均关联到,属于稳定的抗性位点;另有4个标记位点(Xcfd32、Xwmc617、Xmag1263和Xmag2137)同时在2个指标(T2和T3)关联到。优异等位类型分析表明,3个指标均重复检测到的Xgwm285-251bp、Xgwm165-166bp可显著提高小麦幼苗对2,4-滴丁酯、甲基二磺隆的抗性,典型载体品种分别为Niavt14和兰考298;Xcfd32-160bp/158bp、Xwmc617-157bp、Xmag1263-135bp和Xmag2137-189bp等位类型可显著提高小麦芽、根对甲基二磺隆的抗性,载体品种分别为兰考298、周麦16、济南17等;Xcfd76-208bp、Xcfd76-185bp和Xcfd49-131/143bp具有显著提高芽对2,4-滴丁酯的抗性,典型载体品种分别为农大876、新麦18及周麦16。
In this study, a total of 369 SSR markers were used for genotyping 98 elite wheat varieties. The marker-trait associations(MTAs) and excellent alleles for herbicide resistance were identified by general and mixed linear models(GLM and MLM). Three indexes were used for evaluation of herbicide resistance, which were inhibition ratio of 2,4-D to shoot fresh weight(T1), of Mesosulfuron-methyld to shoot fresh weight(T2) and root fresh weight(T3), respectively. The results showed that 37 MTAs were identified by association analysis on chromosomes 1 AL, 1 BL, 1 DL, 2 AS, 2 BS, 3 AL, 3 BL, 3 BS, 4 AL, 4 BL, 5 AL, 5 DL, 5 DS, 6 BL, 6 DL、6 DS, 7 AL, 7 BL, 7 BS, 7 DS and 7 DL. In these MTAs, the two MTAs on 3 BS(Xgwm285) and 4 BL(Xgwm165) could be detected stably for all the three indexes; 4 MTAs(Xcfd32, Xwmc617, Xmag1263 and Xmag2137) were detected for the two indexes(T2 and T3) on 1 D, 4 A and 7 B. The allele's analysis indicated that the alleles Xgwm285-251 bp and Xgwm165-166 bp could improve the resistance of shoot and root to 2,4-DB and Mesosulfuron-methyld, which the typical excellent-allele-carriers are Niavt14 and Lankao 298. The elite alleles of Xcfd32-160 bp/158 bp, Xwmc617-157 bp, Xmag1263-135 bp and Xmag2137-189 bp were useful for improvement of shoot and root resis tance to Mesosulfuron-methyld, which the typical excellent-allele-carriers are Lankao298, Zhoumai16 and Jinan17. The alleles of Xcfd76-208 bp, Xcfd76-185 bp, and Xcfd49-131/143 bp could improve the shoot resistance of Nongda876, Xinmai18 and Zhoumai16 to 2,4-D.
引文
[1]马晓渊.农田杂草抗药性的发生为害、原因与治理[J].杂草科学,2002(1):5-9.
[2]王险峰,范志伟,胡荣娟,等.除草剂药害新进展与解决方法[J].农药,2009,48(5):384-388.
[3]任竹.安徽省小麦药害的产生原因及防治[J].农业灾害研究,2014(10):39-41.
[4]唐建明,王勇,方雅琴.小麦田常用除草剂药害及其规避措施[J].杂草科学,2009,(4):68-71.
[5]李欣,杜丽璞,殷桂香,等.转bar基因小麦和非转基因小麦抗除草剂鉴定方法比较[J].植物遗传资源学报,2012,13(4):596-600.
[6]牛志锋,杜慧玲.不同小麦品种对苯磺隆除草剂的耐药性研究[J].山西农业科学,2008,36(2):28-29.
[7]钱兰娟,袁树忠,高颜.除草剂炔草酯对不同小麦品种幼苗期叶片抗氧化特性的影响[J].麦类作物学报,2014,34(5):674-679.
[8]张芳,慕小倩,戴明.8个栽培小麦品种的化感差异及其对除草剂药效的影响[J].麦类作物学报,2009,29(2):324-329.
[9]张竞秋,高文萱,刘欣.2,4-滴丁酯对小麦根尖细胞有丝分裂染色体畸变的影响[J].东北农业大学学报,2013,44(10):14-17.
[10]WANG S X,ZHU Y L,ZHANG X D,et al.Genome-wide association study for grain yield and related traits in elite wheat varieties and advanced lines using SNP markers[J].PLoS ONE,2017,12(11):e0188662
[11]王克功,任瑞兰,刘玲玲,等.山西主栽小麦品种对世玛和阔世玛的耐药性[J].山西农业科学,2015,43(8):992-994.
[12]朱玉磊,王升星,赵良侠,等.以关联分析发掘小麦整穗发芽抗性基因分子标记[J].作物学报,2014(10):1725-1731,1732.
[13]王升星,朱玉磊,刘鹏,等.小麦次生根数相关分子标记的挖掘[J].麦类作物学报,2014,34(12):1627-1632.
[14]MACKAY I,POWELL W.Methods for linkage disequilibrium mapping in crops[J].Trends Plant Sci,2007,12(2):57-63.
[15]EVANNO G,REGNAUT S,GOUDET J.Detecting the number of clusters of individuals using the software structure:a simulation study[J].Mol Ecol,2005,14(8):2611-2620.
[16]BRADBURY P J,ZHANG Z,KROON D E,et al.TASSEL:software for association mapping of complex traits in diverse samples[J].Bioinformatics,2007,23(19):2633-2635.
[17]文自翔,赵团结,郑永战,等.中国栽培和野生大豆农艺及品质性状与SSR标记的关联分析Ⅱ.优异等位变异的发掘[J].作物学报,2008,34(8):1339-1349.
[18]SOMERS D J,ISAAC P,EDWARDS K.A high-density microsatellite consensus map for bread wheat(Triticum aestivum L.)[J].Theor Appl Genet,2004,109(6):1105-1114.
[19]吴雪平,任锋利,邓天福.三种除草剂对小麦种子生物活性的影响[J].安徽农业科学,2006,34(13):3117,3150.
[20]CHEN H M.Development,chromosome location and genetic mapping of EST-SSR markers in wheat[J].Chinese Sci Bull,2005,50(20):2328-2336.
[21]R?DER M S,KORZUN V,WENDEHAKE K,et al.Amicrosatellite map of wheat[J].Genetics,1998,149(4):2007-2023.
[22]SOURDILLE P,SINGH S,CADALEN T,et al.Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat(Triticum aestivum L.)[J].Funct Integr Genomic,2004,4(1):12-25.