MTA1、CXCL12在非小细胞肺癌中的表达及相关性分析
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摘要
目的:初步探讨非小细胞肺癌(NSCLC)组织中转移相关蛋白1(MTA1)和趋化因子12(CXCL12)的表达并分析其相关性。方法:采用SP免疫组织化学方法检测46例NSCLC组织和20例癌旁正常对照组织中MTA1和CXCL12的表达,分析MTA1和CXCL12之间的相关性及其与NSCLC临床病理参数的关系。结果:NSCLC组织中MTA1和CXCL12的阳性率分别为65.22%和58.70%,均显著高于癌旁对照组织中的15.00%和10.00%(P<0.05);NSCLC组织中MTA1和CXCL12的表达存在正相关关系(r=0.314,P<0.05);NSCLC发生淋巴结转移组MTA1和CXCL12的阳性率分别为78.57%和71.43%,均高于无淋巴结转移组的44.44%和38.89%(P<0.05);MTA1和CXCL12的表达与NSCLC患者性别、年龄、病理类型、分化程度均无明显关系(P>0.05)。结论:MTA1和CXCL12可能在NSCLC发生发展及肿瘤细胞侵袭过程中具有重要作用。
Objective:To investigate metastasis associated protein 1(MTA1)and chemokine 12(CXCL12)expression in the non-small cell lung cancer(NSCLC)tissues and their correlation.Methods:Applying the immunohistochemical technique,detect the expression of MTA1 and CXCL12in 46 cases of NSCLC tissues and 20 cases of normal tissues,to analyze the correlation between MTA1 and CXCL12and their relationship with clinicopathological parameters of NSCLC.Results:The positive rate of MTA1 and CXCL12in NSCLC tissues were 65.22% and 58.70%,higher than the positive rate 15.00%and 10.00%in normal lung tissues(P<0.05);there is a positive correlation between the expression of MTA1 and CXCL12in NSCLC tissues(r=0.314,P<0.05);the positive rate of MTA1 and CXCL12expression in NSCLC tissues with lymph node metastasis were 78.57% and 71.43%,were higher than that in no lymph node metastasis group 44.44% and 38.89%(P<0.05);the expression of MTA1 and CXCL12in NSCLC has no significant correlation with gender,age,pathological type,degree of differentiation(P>0.05).Conclusion:MTA1and CXCL12 may play an important role in the development of NSCLC and the invasion of tumor cells.
Objective:To investigate metastasis associated protein 1(MTA1)and chemokine 12(CXCL12)expression in the non-small cell lung cancer(NSCLC)tissues and their correlation.Methods:Applying the immunohistochemical technique,detect the expression of MTA1 and CXCL12in 46 cases of NSCLC tissues and 20 cases of normal tissues,to analyze the correlation between MTA1 and CXCL12and their relationship with clinicopathological parameters of NSCLC.Results:The positive rate of MTA1 and CXCL12in NSCLC tissues were 65.22% and 58.70%,higher than the positive rate 15.00%and 10.00%in normal lung tissues(P<0.05);there is a positive correlation between the expression of MTA1 and CXCL12in NSCLC tissues(r=0.314,P<0.05);the positive rate of MTA1 and CXCL12expression in NSCLC tissues with lymph node metastasis were 78.57% and 71.43%,were higher than that in no lymph node metastasis group 44.44% and 38.89%(P<0.05);the expression of MTA1 and CXCL12in NSCLC has no significant correlation with gender,age,pathological type,degree of differentiation(P>0.05).Conclusion:MTA1and CXCL12 may play an important role in the development of NSCLC and the invasion of tumor cells.
引文
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[2]Yu X,Shi W,Zhang Y,et al.CXCL12/CXCR4axis induced miR-125bpromotes invasion and confers 5-fluorouracil resistance through enhancing autophagy in colorectal cancer[J].Sci Rep,2017,7:42226.
[3]张用,毕建平,皮国良,等.国际肺癌研究协会第八版国际肺癌TNM分期修订稿解读[J].肿瘤防治研究,2016,43(4):313.
[4]Sen N,Gui B,Kumar R.Role of MTA1in Cancer Progression and Metastasis[J].Cancer Metastasis Rev,2014,33(4):879-889.
[5]Miyashita T,Tajima H,Munemoto M,et al.Impact of histone deacetylase 1and metastasis-associated gene 1expression in esophageal carcinogenesis[J].Oncol Lett,2014,8(2):758-764.
[6]Zhang H,Zhu X,Li N,et al.miR-125a-3p targets MTA1to suppress NSCLC cell proliferation,migration,and invasion[J].Acta Biochim Biophys Sin(Shanghai),2015,47(7):496-503.
[7]Kumar R,Balasenthil S,Manavathi B,et al.Metastasis-associated protein 1and its short form variant stimulates Wnt1transcription through promoting its derepression from Six3corepressor[J].Cancer Res,2010,70(16):6649-6658.
[8]Domanska UM,Kruizinga RC,Nagengast WB,et al.A review on CXCR4/CXCL12axis in oncology:no place to hide[J].Eur J Cancer,2013,49(1):219-230.
[9]Ghosh MC,Makena PS,Gorantla V,et al.CXCR4regulates migration of lung alveolar epithelial cells through activation of Rac1and matrix metalloproteinase-2[J].Am J Physiol Lung Cell Mol Physiol,2012,302(9):L846-L856.
[10]Ghanem I,Riveiro ME,Paradis V,et al.Insights on the CXCL12-CXCR4axis in hepatocellular carcinoma carcinogenesis[J].Am J Transl Res,2014,6(4):340-352.