摘要
目的探讨肿瘤转移相关因子MTA1、干细胞转录因子SOX2在食管癌组织中的表达、二者的相关性以及与预后之间的相互关系。方法分别采用免疫细胞化学、Real-time PCR、Western blot方法检测来源于我院肿瘤科2002年6月~2005年6月的144例食管癌组织标本中MTA1、SOX2的表达,并结合以上患者10年随访资料分析其表达与临床病理及食管癌预后的关系。结果 144例免疫组化结果显示,MTA1、SOX2均在细胞核中表达。MTA1阴性或弱阳性率、中等阳性率与强阳性率分别为58.34%、27.08%与14.58%;SOX2阴性或弱阳性率、中等阳性率与强阳性率分别为65.28%、22.92%与11.81%。MTA1、SOX2在食管癌组织中的表达与T分级、临床分期有关,与肿瘤患者的总生存期呈负相关。结论肿瘤转移相关因子MTA1、干细胞转录因子SOX2在食管癌组织中均有不同程度的表达,且存在相关性。MTA1、SOX2在食管癌组织中同时存在高表达与预后不良相关。
Objective To study the expression of tumor metastasis related gene MTA1 and stem cell transcription factor SOX2 in esophageal cancer tissue,the correlation of MTA1 and SOX2,as well as the relationship between them and prognosis,and to provide a theoretical basis for clinical early diagnosis and prognosis. Methods The expression of MTA1 and SOX2 were detected with immunocytochemistry,Real-time PCR,Western blot method in 144 samples of esophageal carcinoma tissues. Combined with follow-up data more than10 years,the relationship between the expression of MTA1 and SOX2,the correlation with clinical pathologic features as well as its relationship with prognosis were analyzed. Results The result showed that both MTA1 and SOX2 were expressed in the cell nucleus of esophageal cancer. The negative or weakly positive rate,moderate positive rate and strong positive rate was 58.34%,27.08% and 14.58% respectively in MTA1 expression,and was 65. 28%,22. 92% and 11. 81% respectively in SOX2 expression. The expression of both MTA1 and SOX2 were related to T staging and clinical stage in esophageal cancer tissues,and negative correlated with overall survival span in esophageal cancer cases. Conclusions Tumor metastasis related factors MTA1 and stem cell transcription factor SOX2 were expressed with different degrees in esophageal cancer tissues,and there was correlation between them. The high expression of MTA1 and SOX2 simultaneously in esophageal cancer tissues were associated with poor prognosis.
引文
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