基因Mta1及其蛋白表达与头颈部鳞状细胞癌浸润转移的关系
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摘要
目的观察肿瘤转移相关基因Mta1(metastasis associated gene 1)及其蛋白在头颈部鳞状细胞癌中的表达,并探讨其临床意义。方法对86例头颈部鳞状细胞癌组织和20例头颈部正常组织用逆转录-PCR(RT-PCR)方法检测Mta1 m RNA表达,用免疫组化检测Mta1蛋白表达。结果 Mta1 m RNA在86例头颈部鳞状细胞癌组织中平均表达水平(1.72±0.56)%与头颈部正常组织(0.93±0.68)%比较,差异具有显著性(P<0.01);Mta1蛋白在头颈部鳞状细胞癌组织阳性率(65.12%,56/86)与对照组(15%,3/20)比较,差异具有显著性(P<0.01)。Mta1 m RNA及其蛋白在下咽癌、鼻咽癌、颈部皮肤鳞癌、淋巴结癌、扁桃体癌中平均表达水平及阳性率分别与头颈部正常组织比较,差异具有显著性(P<0.01);Mta1 m RNA及蛋白在头颈部鳞状细胞癌中表达及阳性率与高分化、中分化及低分化等分化程度呈正相关(P<0.05)。结论 Mta1 m RNA及其蛋白表达与头颈部鳞状细胞癌浸润转移关系密切,提示该基因可成为预测头颈部鳞状细胞癌转移和预后有价值的指标之一。
Objective To observe the expression of Metastasis Associated Gene(Mta1) and its protein in head and neck squamous cell carcinoma and its clinical significance. Methods Mta1 m RNA expression was detected by reverse transcription-PCR(RT-PCR) in 86 head and neck squamous cell carcinoma tissues and 20 normal head and neck tissues.The expression of Mta1 protein was detected by immunohistochemistry. Results The average expression level of Mta1 m RNA in 86 cases of head and neck squamous cell carcinoma was(1.72±0.56) compared with(0.93±0.68) in head and neck tissues. The difference was statistically significant(P<0.01). The positive rate of Mta1 protein in squamous cell carcinoma of the head and neck(65.12%, 56/86) was significantly higher than that of the control group(15%, 3/20)(P<0.01). The expression and positive rate of Mta1 m RNA and its protein in hypopharyngeal carcinoma, nasopharyngeal carcinoma, neck cutaneous squamous cell carcinoma, lymph node carcinoma, and tonsillar carcinoma were significantly different from those of normal tissues of head and neck, respectively(P<0.01). The expression and positive rate of Mta1 m RNA and protein in head and neck squamous cell carcinoma were positively correlated with the degree of differentiation of well differentiated, moderately differentiated and poorly differentiated(P<0.05). Conclusion The expression of Mta1 m RNA and its protein is closely related to the invasion and metastasis of head and neck squamous cell carcinoma, suggesting that this gene may be useful for predicting the metastasis and prognosis of head and neck squamous cell carcinoma.
Objective To observe the expression of Metastasis Associated Gene(Mta1) and its protein in head and neck squamous cell carcinoma and its clinical significance. Methods Mta1 m RNA expression was detected by reverse transcription-PCR(RT-PCR) in 86 head and neck squamous cell carcinoma tissues and 20 normal head and neck tissues.The expression of Mta1 protein was detected by immunohistochemistry. Results The average expression level of Mta1 m RNA in 86 cases of head and neck squamous cell carcinoma was(1.72±0.56) compared with(0.93±0.68) in head and neck tissues. The difference was statistically significant(P<0.01). The positive rate of Mta1 protein in squamous cell carcinoma of the head and neck(65.12%, 56/86) was significantly higher than that of the control group(15%, 3/20)(P<0.01). The expression and positive rate of Mta1 m RNA and its protein in hypopharyngeal carcinoma, nasopharyngeal carcinoma, neck cutaneous squamous cell carcinoma, lymph node carcinoma, and tonsillar carcinoma were significantly different from those of normal tissues of head and neck, respectively(P<0.01). The expression and positive rate of Mta1 m RNA and protein in head and neck squamous cell carcinoma were positively correlated with the degree of differentiation of well differentiated, moderately differentiated and poorly differentiated(P<0.05). Conclusion The expression of Mta1 m RNA and its protein is closely related to the invasion and metastasis of head and neck squamous cell carcinoma, suggesting that this gene may be useful for predicting the metastasis and prognosis of head and neck squamous cell carcinoma.
引文
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[9]张裘,万涛.食管癌组织MTA1表达与临床特征及预后的关系[J].临床与实验医学杂志,2017,17(9):873-875.
[10]周敬淳,刘明,张伟,等.Ezrin与MTA1在鼻咽癌组织中的表达研究[J].白求恩医学杂志,2015,16(3):296-297.
[11]Hamatsu T,Rikimaru T,Yamashita Y,et al.The role of MTA1 gene expression in human hepatocellμlar carcinoma[J].Oncol Rep,2003,10(3):599-604.
[12]程阳,唐桥斐,赵丽妮,等.MTA1基因表达与下咽癌颈淋巴结转移的关系[J].沈阳医学院学报,2015,12(1):28-30.
[13]Sasaki H,Moriyama S,Nakashima Y,et al.Expression of the MTA1 m RNA in advanced lung cancer[J].Lung Cancer,2002,35(2):149-154.
[14]王会河,莫立根,黄光武,等.mtal基因和癌肿转移[J].中国医学文摘.肿瘤学,2004:147-149.
[15]王会河,黄光武,韦福依,等.基因MTA1在喉癌中的表达和意义[J].临床耳鼻咽喉科杂志,2006,12(3):113-116.
[2]Jemal A,Siegel R,Xu J,et al.Cancer statistics[J].CA Cancer J Clin,2010,60(5):277-300.
[3]杜江,张林.非小细胞肺癌中Tia m-1的表达及其临床病理意义[J].中国医科大学学报,2005,34(4):329-331.
[4]Hamatsu T,Rikimaru T,Yamashita Y,et al.The role of MTA1 gene expression in human hepatocellμlar carcinoma[J].Oncol Rep,2003,10(3):599-604.
[5]Toh Y,Pencil SD,Nicolson GL,et al.Analysis of the complete sequence of the novel metastasis-associated candidate gene,mta1,differentially expressed in mammary adenocarcinoma and breast cancer cell lines[J].Gene,1995,159(1):97-104.
[6]Toh Y,Pencil SD,Nicolson GL,et al.A novel candidate metastasis-associated gene mta1,differentially expressed in highly metastatic mammary adenocarcinoma cell lines.c DNA cloning,expression,and protein analyses[J].J Biol Chem,1994,269(37):22958-22963.
[7]罗云飞,王固新.MTA1基因研究进展[J].世界临床医学,2016,9(1):31-34.
[8]张海利,温树信,高伟,等.MTA1在喉鳞癌中的表达及其与淋巴转移关系的研究[J].中国医药与临床,2016,7(10):1460-1461.
[9]张裘,万涛.食管癌组织MTA1表达与临床特征及预后的关系[J].临床与实验医学杂志,2017,17(9):873-875.
[10]周敬淳,刘明,张伟,等.Ezrin与MTA1在鼻咽癌组织中的表达研究[J].白求恩医学杂志,2015,16(3):296-297.
[11]Hamatsu T,Rikimaru T,Yamashita Y,et al.The role of MTA1 gene expression in human hepatocellμlar carcinoma[J].Oncol Rep,2003,10(3):599-604.
[12]程阳,唐桥斐,赵丽妮,等.MTA1基因表达与下咽癌颈淋巴结转移的关系[J].沈阳医学院学报,2015,12(1):28-30.
[13]Sasaki H,Moriyama S,Nakashima Y,et al.Expression of the MTA1 m RNA in advanced lung cancer[J].Lung Cancer,2002,35(2):149-154.
[14]王会河,莫立根,黄光武,等.mtal基因和癌肿转移[J].中国医学文摘.肿瘤学,2004:147-149.
[15]王会河,黄光武,韦福依,等.基因MTA1在喉癌中的表达和意义[J].临床耳鼻咽喉科杂志,2006,12(3):113-116.