MUC1和MUC5AC在鼻咽癌中的表达及临床意义
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摘要
目的分析黏蛋白1(MUC1)和黏蛋白5AC(MUC5AC)在鼻咽癌组织中的表达及临床意义。方法收集2015年1月至2018年6月在空军军医大学唐都医院治疗的67例鼻咽癌患者的组织标本,采用免疫组化S-P法检测鼻咽癌组织中MUC1和MUC5AC的表达水平。结果鼻咽癌中MUC1的阳性表达率为91.04%,且在不同组织学病理类型中的表达差异有统计学意义(P<0.05);MUC5AC表达的阳性率为37.31%,且在不同病理类型的表达差异无统计学意义(P>0.05)。MUC1和MUC5AC的表达与颈部淋巴结转移、TNM分期有关(P<0.05),与患者的年龄和性别无关(P>0.05)。鼻咽癌中MUC1和MUC5AC蛋白之间具有很强的关联性(r=0.48,P<0.05)。结论鼻咽癌中MUC1在不同的组织学病理类型中差异表达,MUC1和MUC5AC的表达与颈部淋巴结转移、TNM分期相关。MUC1和MUC5AC之间存在紧密联系。
Objective To investigate the expression of MUC1 and MUC5 AC in nasopharyngeal carcinoma and their clinical significance. Methods MU C1 and MUC5 AC were detected by immunohistochemical S-P method in 67 nasopharyngeal carcinoma tissues collected from January 2015 to June 2018. Results The positive expression rate of MUC1 in nasopharyngeal carcinoma was 91.04%, and the difference was statistically significant in different histopathological types(P<0.05). The positive expression rate of MUC5 AC was 37.31%, and there was no significant difference in different pathological types(P>0.05). The expression of MU C1 and MUC5 was related to the metastasis of cervical lymph node and TNM staging(P<0.05), but not with age and sex(P>0.05). The correlation analysis showed a strong correlation between MUC 1 and MUC5 AC proteins in nasopharyngeal carcinoma. Conclusion The expression of MUC 1 in nasopharyngeal carcinoma was differentially expressed in different histological types, and the expression of MUC1 and MUC5 AC was related to the metastasis of cervical lymph nodes and the staging of TNM. The correlation analysis showed that there was a strong correlation between MUC1 and MUC5 AC protein in nasopharyngeal carcinoma.
Objective To investigate the expression of MUC1 and MUC5 AC in nasopharyngeal carcinoma and their clinical significance. Methods MU C1 and MUC5 AC were detected by immunohistochemical S-P method in 67 nasopharyngeal carcinoma tissues collected from January 2015 to June 2018. Results The positive expression rate of MUC1 in nasopharyngeal carcinoma was 91.04%, and the difference was statistically significant in different histopathological types(P<0.05). The positive expression rate of MUC5 AC was 37.31%, and there was no significant difference in different pathological types(P>0.05). The expression of MU C1 and MUC5 was related to the metastasis of cervical lymph node and TNM staging(P<0.05), but not with age and sex(P>0.05). The correlation analysis showed a strong correlation between MUC 1 and MUC5 AC proteins in nasopharyngeal carcinoma. Conclusion The expression of MUC 1 in nasopharyngeal carcinoma was differentially expressed in different histological types, and the expression of MUC1 and MUC5 AC was related to the metastasis of cervical lymph nodes and the staging of TNM. The correlation analysis showed that there was a strong correlation between MUC1 and MUC5 AC protein in nasopharyngeal carcinoma.
引文
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[2]严晓菊,方舒,黄国威,等.疑似鼻咽癌的成人鼻咽部肿物的临床研究[J].中华耳鼻咽喉头颈外科杂志,2018,53(7):519-523.Yan XJ,Fang S,Huang GW,et al.Clinical study of nasopharyngeal masses with suspicion of nasopharyngeal carcinoma in adult patients[J].Chin J Otorhinolaryngol Head Neck Surg,2018,53(7):519-523.
[3]Dhanisha SS,Guruvayoorappan C,Drishya S,et al.Mucins:Structural diversity,biosynthesis,its role in pathogenesis and as possible therapeutic targets[J].Crit Rev Oncol Hematol,2018,122:98-122.
[4]Hou R,Jiang L,Liu D,et al.Lewis(y)antigen promotes the progression of epithelial ovarian cancer by stimulating MUC1expression[J].Int J Mol Med,2017,40(2):293-302.
[5]Bae CH,Choi YS,Song SY,et al.Escherichia coli-derived and Staphylococcus aureus-derived extracellular vesicles induce MUC5AC expression via extracellular signal related kinase 1/2and p38 mitogen-activated protein kinase in human airway epithelial cells[J].Int Forum Allergy Rhinol,2017,7(1):91-98.
[6]Bhoge A,Khandeparkar SGS,Joshi AR,et al.Immunohistochemical study of MUC1 and MUC5AC expression in gall bladder lesions[J].J Clin Diagn Res,2017,11(7):EC12-EC16.
[7]Wang SB,Zhang C,Xu XC,et al.Early growth response factor 1is essential for cigarette smoke-induced MUC5AC expression in human bronchial epithelial cells[J].Biochem Biophys Res Commun,2017,490(2):147-154.
[8]宋振国,张占东,苏永林,等.MUC1和MUC5AC表达水平与食管癌预后的相关性[J].热带医学杂志,2018,18(7):902-906.Song ZG,Zhang ZD,Su YL,et al.The correlation between the expression levels of MUC1 and MUC5AC and the prognosis of esophageal cancer[J].J Trop Med,2018,18(7):902-906.
[9]Seidal T,Balaton AJ,Battifora H.Interpretation and quantification of immunostains[J].Am J Surg Pathol,2001,25(9):1204-1207.
[10]Ablamowicz AF,Nichols JJ.Ocular surface membraneassociated mucins[J].Ocul Surf,2016,14(3):331-341.
[11]Lanzel EA,Pourian A,Sousa Melo SL,et al.Expression of Membrane-Bound Mucins and p63 in Distinguishing Mucoepidermoid Carcinoma from Papillary Cystadenoma[J].Head Neck Pathol,2016,10(4):521-526.
[12]Amat CB,Motta JP,Fekete E,et al.Cysteine ProteaseDependent Mucous Disruptions and Differential Mucin Gene Expression in Giardia duodenalis Infection[J].Am J Pathol,2017,187(11):2486-2498.
[13]Mall AS,Habte H,Mthembu Y,et al.Mucus and Mucins:do they have a role in the inhibition of the human immunodeficiency virus[J]?Virol J,2017,14(1):192.
[14]Kumar S,Cruz E,Joshi S,et al.Genetic variants of mucins:unexplored conundrum[J].Carcinogenesis,2017,38(7):671-679.
[15]Sun R,Wang X,Li X.Correlation Analysis of Nasopharyngeal Carcinoma TNM Staging with Serum EA IgA and VCA IgA in EBVand VEGF-C and-D[J].Med Sci Monit,2015,21:2105-2109.
[16]潘莉莉,黄华艺.表皮生长因子受体与鼻咽癌的关系[J].分子诊断与治疗,2017,9(2):121-126.Pan LL,Huang HY.Epidermal growth factor receptor and nasopharyngeal carcinoma[J].J Mol Diagn Ther,2017,9(2):121-126.
[17]Kato K,Zemskova MA,Hanss AD,et al.MUC1 deficiency exacerbates pulmonary fibrosis in a mouse model of silicosis[J].Biochem Biophys Res Commun,2017,493(3):1230-1235.
[18]Zhao P,Meng M,Xu B,et al.Decreased expression of MUC1induces apoptosis and inhibits migration in pancreatic cancer PANC-1 cells via regulation of Slug pathway[J].Cancer Biomark,2017,20(4):469-476.
[19]Syrkina MS,Maslakova AA,Potashnikova DM,et al.Dual Role of the Extracellular Domain of Human Mucin MUC1 in Metastasis[J].J Cell Biochem,2017,118(11):4002-4011.
[20]Taylor-Papadimitriou J,Burchell JM,Graham R,et al.Latest developments in MUC1 immunotherapy[J].Biochem Soc Trans,2018,46(3):659-668.
[21]Magalhaes A,Rossez Y,Robbe-Masselot C,et al.MUC5ACgastric mucin glycosylation is shaped by FUT2 activity and functionally impacts Helicobacter pylori binding[J].Sci Rep,2016,6:25575.
[22]Balmana M,Duran A,Gomes C,et al.Analysis of sialyl-Lewis x on MUC5AC and MUC1 mucins in pancreatic cancer tissues[J].Int J Biol Macromol,2018,112:33-45.2018-09-19
[2]严晓菊,方舒,黄国威,等.疑似鼻咽癌的成人鼻咽部肿物的临床研究[J].中华耳鼻咽喉头颈外科杂志,2018,53(7):519-523.Yan XJ,Fang S,Huang GW,et al.Clinical study of nasopharyngeal masses with suspicion of nasopharyngeal carcinoma in adult patients[J].Chin J Otorhinolaryngol Head Neck Surg,2018,53(7):519-523.
[3]Dhanisha SS,Guruvayoorappan C,Drishya S,et al.Mucins:Structural diversity,biosynthesis,its role in pathogenesis and as possible therapeutic targets[J].Crit Rev Oncol Hematol,2018,122:98-122.
[4]Hou R,Jiang L,Liu D,et al.Lewis(y)antigen promotes the progression of epithelial ovarian cancer by stimulating MUC1expression[J].Int J Mol Med,2017,40(2):293-302.
[5]Bae CH,Choi YS,Song SY,et al.Escherichia coli-derived and Staphylococcus aureus-derived extracellular vesicles induce MUC5AC expression via extracellular signal related kinase 1/2and p38 mitogen-activated protein kinase in human airway epithelial cells[J].Int Forum Allergy Rhinol,2017,7(1):91-98.
[6]Bhoge A,Khandeparkar SGS,Joshi AR,et al.Immunohistochemical study of MUC1 and MUC5AC expression in gall bladder lesions[J].J Clin Diagn Res,2017,11(7):EC12-EC16.
[7]Wang SB,Zhang C,Xu XC,et al.Early growth response factor 1is essential for cigarette smoke-induced MUC5AC expression in human bronchial epithelial cells[J].Biochem Biophys Res Commun,2017,490(2):147-154.
[8]宋振国,张占东,苏永林,等.MUC1和MUC5AC表达水平与食管癌预后的相关性[J].热带医学杂志,2018,18(7):902-906.Song ZG,Zhang ZD,Su YL,et al.The correlation between the expression levels of MUC1 and MUC5AC and the prognosis of esophageal cancer[J].J Trop Med,2018,18(7):902-906.
[9]Seidal T,Balaton AJ,Battifora H.Interpretation and quantification of immunostains[J].Am J Surg Pathol,2001,25(9):1204-1207.
[10]Ablamowicz AF,Nichols JJ.Ocular surface membraneassociated mucins[J].Ocul Surf,2016,14(3):331-341.
[11]Lanzel EA,Pourian A,Sousa Melo SL,et al.Expression of Membrane-Bound Mucins and p63 in Distinguishing Mucoepidermoid Carcinoma from Papillary Cystadenoma[J].Head Neck Pathol,2016,10(4):521-526.
[12]Amat CB,Motta JP,Fekete E,et al.Cysteine ProteaseDependent Mucous Disruptions and Differential Mucin Gene Expression in Giardia duodenalis Infection[J].Am J Pathol,2017,187(11):2486-2498.
[13]Mall AS,Habte H,Mthembu Y,et al.Mucus and Mucins:do they have a role in the inhibition of the human immunodeficiency virus[J]?Virol J,2017,14(1):192.
[14]Kumar S,Cruz E,Joshi S,et al.Genetic variants of mucins:unexplored conundrum[J].Carcinogenesis,2017,38(7):671-679.
[15]Sun R,Wang X,Li X.Correlation Analysis of Nasopharyngeal Carcinoma TNM Staging with Serum EA IgA and VCA IgA in EBVand VEGF-C and-D[J].Med Sci Monit,2015,21:2105-2109.
[16]潘莉莉,黄华艺.表皮生长因子受体与鼻咽癌的关系[J].分子诊断与治疗,2017,9(2):121-126.Pan LL,Huang HY.Epidermal growth factor receptor and nasopharyngeal carcinoma[J].J Mol Diagn Ther,2017,9(2):121-126.
[17]Kato K,Zemskova MA,Hanss AD,et al.MUC1 deficiency exacerbates pulmonary fibrosis in a mouse model of silicosis[J].Biochem Biophys Res Commun,2017,493(3):1230-1235.
[18]Zhao P,Meng M,Xu B,et al.Decreased expression of MUC1induces apoptosis and inhibits migration in pancreatic cancer PANC-1 cells via regulation of Slug pathway[J].Cancer Biomark,2017,20(4):469-476.
[19]Syrkina MS,Maslakova AA,Potashnikova DM,et al.Dual Role of the Extracellular Domain of Human Mucin MUC1 in Metastasis[J].J Cell Biochem,2017,118(11):4002-4011.
[20]Taylor-Papadimitriou J,Burchell JM,Graham R,et al.Latest developments in MUC1 immunotherapy[J].Biochem Soc Trans,2018,46(3):659-668.
[21]Magalhaes A,Rossez Y,Robbe-Masselot C,et al.MUC5ACgastric mucin glycosylation is shaped by FUT2 activity and functionally impacts Helicobacter pylori binding[J].Sci Rep,2016,6:25575.
[22]Balmana M,Duran A,Gomes C,et al.Analysis of sialyl-Lewis x on MUC5AC and MUC1 mucins in pancreatic cancer tissues[J].Int J Biol Macromol,2018,112:33-45.2018-09-19