食源性致病微生物危害风险及其防控用抗菌生物活性肽研究进展
|
摘要
食源致病微生物是污染食品、食用农产品的主要生物危害物,严重威胁人类及动物健康;同时也给食品、食用农产品产业造成巨大经济损失,是制约产业健康可持续发展的关键危害因素。传统依赖抗生素对致病微生物的防控效果显著,但由于抗生素长期的不合理使用甚至是滥用所诱发产生的致病微生物耐药性,以及对人类和动物体内脏器、肠道组织、神经和代谢系统正常功能构成的潜在危害性等问题也同样突出。抗菌生物活性肽作为具有抗菌功能的生物多肽类蛋白质,被认为是对人类、动物以及生态环境友好的抗菌物质,有望替代抗生素用于致病微生物的防控,现已成为探索新型安全抗菌类药物研发的热点。全面梳理了食源致病微生物种类、污染特点及其主要危害风险;系统概述了抗菌生物活性肽主要类型、来源及其创制技术;着重介绍了当前抗菌生物活性肽在食源致病微生物防控上的研究状况,并深入探讨了其应用前景、存在问题及拟解决对策,旨在为食品、食用农产品中食源性致病微生物筛查以及靶向防控技术提供最新的有参考价值的文献资料和创新思路。
Food-borne pathogenic microorganisms are the main biological hazards that contaminate food and edible-agricultural products and seriously threaten the health of humans and animals. At the same time,they also cause huge economic losses to food and edibleagricultural products industry,which is the key factor of restricting their development. Traditional dependence on antibiotics has a significant effect on pathogenic microorganism prevention and control. However,due to long-term unreasonable use or even abuse of antibiotics,the drug resistance of pathogenic microorganisms,as well as potential harmfulness to normal functions of internal organs,intestinal tissues,nervous and metabolic systems in humans and animals,are also prominent. Antimicrobial bioactive peptides,as biological peptides with antimicrobial functions,are considered as antimicrobial substances friendly to humans,animals and ecological environment,and they expected to replace antibiotics in pathogenic microorganism prevention and control. Now,antimicrobial bioactive peptide has become a hot spot to explore new and safe antibacterial drugs. In this paper,we comprehensively sort out their types,pollution characteristics and main hazard risks of food-borne pathogenic microorganisms,and systematically summarize their main types and source of antimicrobial bioactive peptides and their creation techniques. Then we emphatically introduce the current research status of antimicrobial bioactive peptides for food-borne pathogenic microorganism prevention and control. Finally,we discuss their application prospect,existing issues and countermeasures. To sum up,this paper is to provide the latest references or innovative idea for screening as well as prevention and control of food-borne pathogenic microorganisms in food and edible agricultural products.
Food-borne pathogenic microorganisms are the main biological hazards that contaminate food and edible-agricultural products and seriously threaten the health of humans and animals. At the same time,they also cause huge economic losses to food and edibleagricultural products industry,which is the key factor of restricting their development. Traditional dependence on antibiotics has a significant effect on pathogenic microorganism prevention and control. However,due to long-term unreasonable use or even abuse of antibiotics,the drug resistance of pathogenic microorganisms,as well as potential harmfulness to normal functions of internal organs,intestinal tissues,nervous and metabolic systems in humans and animals,are also prominent. Antimicrobial bioactive peptides,as biological peptides with antimicrobial functions,are considered as antimicrobial substances friendly to humans,animals and ecological environment,and they expected to replace antibiotics in pathogenic microorganism prevention and control. Now,antimicrobial bioactive peptide has become a hot spot to explore new and safe antibacterial drugs. In this paper,we comprehensively sort out their types,pollution characteristics and main hazard risks of food-borne pathogenic microorganisms,and systematically summarize their main types and source of antimicrobial bioactive peptides and their creation techniques. Then we emphatically introduce the current research status of antimicrobial bioactive peptides for food-borne pathogenic microorganism prevention and control. Finally,we discuss their application prospect,existing issues and countermeasures. To sum up,this paper is to provide the latest references or innovative idea for screening as well as prevention and control of food-borne pathogenic microorganisms in food and edible agricultural products.
引文
[1]Havelaar AH, Kirk MD, Torgerson PR, et al. World health organization global estimates and regional comparisons of the burden of foodborne disease in 2010[J]. PLoS Med, 2015, 12(12):e1001923.
[2]MembréJM, Guillou S. Latest developments in foodborne pathogen risk assessment[J]. Curr Opin Food Sci, 2016(8):120-126.
[3]Courvalin P. Why is antibiotic resistance a deadly emerging disease?[J]. Clin Microbiol Infect, 2016, 22(5):405-407.
[4]Yoo SM, Lee SY. Optical biosensors for the detection of pathogenic microorganisms[J]. Trends Biotechnol, 2016, 34(1):7-25.
[5]Costa JP, Cova M, Ferreira R, et al. Antimicrobial peptides:an alternative for innovative medicines?[J]. Appl Microbiol Biotechnol, 2015, 99(5):2023-2040.
[6]Wang G, Mishra B, Lau K, et al. Antimicrobial peptides in2014[J]. Pharmaceuticals, 2015, 8(1):123-150.
[7]Hilpert K, Hancock RE. Use of luminescent bacteria for rapid screening and characterization of short cationic antimicrobial peptidessynthesizedoncelluloseusingpeptidearray technology[J]. Nat Protoc, 2007, 2(7):1652-1660.
[8]Castel G, Chteoui M, Heyd B, et al. Phage display of combinatorial peptide libraries:application to antiviral research[J]. Molecules,2011, 16(5):3499-3518.
[9]Tucker AT, Leonard SP, DuBois CD, et al. Discovery of nextgeneration antimicrobials through bacterial self-screening of surfacedisplayed peptide libraries[J]. Cell, 2018, 172(3):618-628.
[10]Li D, Butot S, Zuber S, et al. Monitoring of foodborne viruses in berries and considerations on the use of RT-PCR methods in surveillance[J]. Food Control, 2018, 89:235-240.
[11]Jones TH, Muehlhauser V. Frequency of hepatitis E virus,rotavirus and porcine enteric calicivirus at various stages of pork carcass processing in two pork processing plants[J]. Int J Food Microbiol, 2017, 259:29-34.
[12]Zhang Y, Liu X, Wang Y, et al. Antibacterial activity and mechanism of cinnamon essential oil against Escherichia coli and Staphylococcus aureus[J]. Food Control, 2016, 59:282-289.
[13]Kirk MD, Pires SM, Black RE, et al. World health organization estimates of the global and regional disease burden of 22 foodborne bacterial, protozoal and viral diseases[J]. PLoS Med, 2015, 12(12):e1001921.
[14]Raiola A, Tenore GC, Manyes L, et al. Risk analysis of main mycotoxins occurring in food for children:an overview[J]. Food Chem Toxicol, 2015, 84:169-180.
[15]Frisvad JC. A critical review of producers of small lactone mycotoxins:patulin, penicillic acid and moniliformin[J]. World Mycotoxin J, 2018, 11(1):73-100.
[16]LuoY,LiuX,LiJ.Updatingtechniquesoncontrolling mycotoxins-a review[J]. Food Control, 2018, 89:123-132.
[17]Stiff RE, Davies AP, Mason BW, et al. Long-term health effects after resolution of acute cryptosporidium parvum infection:a 1-year follow-up of outbreak-associated cases[J]. J Med Microbiol,2017, 66(11):1607-1611.
[18]Waldram A, Vivancos R, Hartley C, et al. Prevalence of giardia infection in households of giardia cases and risk factors for household transmission[J]. BMC Infect Dis, 2017, 17(1):e486.
[19]Rostami A, Khazan H, Kia EB, et al. Molecular identification of Trichinella spp. in wild boar and serological survey of high-risk populations in Iran[J]. Food Control, 2018, 90:40-47.
[20]Ssemanda JN, Reij MW, Middendorp G, et al. Foodborne pathogens and their risk exposure factors associated with farm vegetables in Rwanda[J]. Food Control, 2018, 89:86-96.
[21]Chung PY, Khanum R. Antimicrobial peptides as potential antibiofilm agents against multidrug-resistant bacteria[J]. J Microbiol Immunol Infect, 2017, 50(4):405-410.
[22]Huang JX, Bishop-Hurley SL, Cooper MA. Development of antiinfectives using phage display:biological agents against bacteria,viruses and parasites[J]. Antimicrob Agents Ch, 2012, 56(9):4569-4582.
[23]Murakami M, Dorschner RA, Stern LJ, et al. Expression and secretion of cathelicidin antimicrobial peptides in murine mammary glands and human milk[J]. Pediatr Res, 2005, 57(1):10-15.
[24]Gao W, Xing L, Qu P, et al. Identification of a novel cathelicidin antimicrobial peptide from ducks and determination of its functional activity and antibacterial mechanism[J]. Sci Rep, 2015, 5:e17260.
[25]Li T, Wang X, Wang Y, et al. Characterization of antimicrobial peptides isolated from the processing by products of african catfish clarias gariepinus[J]. Int J Pept Res Ther, 2016, 23(2):227-233.
[26]Nayak T, Mandal SM, Neog K, et al. Characterization of a gloverinlike antimicrobial peptide isolated from muga silkworm, antheraea assamensis[J]. Int J Pept Res Ther, 2018, 24(4):337-346.
[27]LeeH,HwangJS,Lee J,etal.Scolopendin2,a cationic antimicrobial peptide from centipede, and its membrane-active mechanism[J]. Biochim Biophys Acta, 2015, 1848(2):634-642.
[28]Memarpoor-Yazdi M, Zare-Zardini H, Asoodeh A. A novel antimicrobialpeptidederivedfromtheinsectpaederus dermatitis[J]. Int J Pept Res Ther, 2012, 19(2):99-108.
[29]Tang W, Yuan H, Zhang H, et al. An antimicrobial peptide screened from casein hydrolyzate by Saccharomyces cerevisiae cell membrane affinity method[J]. Food Control, 2015, 50:413-422.
[30]Pei J, Jiang L. Antimicrobial peptide from mucus of Andrias davidianus:screening and purification by magnetic cell membrane separation technique[J]. Int J Antimicrob Agents, 2017, 50(1):41-46.
[31]TangW,ZhangH,WangL,etal.Targetedseparationof antibacterial peptide from protein hydrolysate of anchovy cooking wastewater by equilibrium dialysis[J]. Food Chem, 2015, 168:115-123.
[32]Pini A, Giuliani A, Falciani C, et al. Antimicrobial activity of novel dendrimeric peptides obtained by phage display selection and rational modification[J]. Antimicrob. Agents Ch, 2005, 49(7):2665-2672.
[33]Rao SS, Mohan KV, Atreya CD. A peptide derived from phage display library exhibits antibacterial activity against E. coli and Pseudomonas aeruginosa[J]. PLoS One, 2013, 8(2):e56081.
[34]Bishop-Hurley SL, Rea PJ, McSweeney CS. Phage-displayed peptidesselectedforbindingtocampylobacterjejuniare antimicrobial[J]. Protein Eng Des Sel, 2010, 23(10):751-757.
[35]Zoeiby AE. Identification of novel inhibitors of Pseudomonas aeruginosa MurC enzyme derived from phage-displayed peptide libraries[J]. J Antimicrob Chemoth, 2003, 51(3):531-543.
[36]Tanaka T, Kokuryu Y, Matsunaga T. Novel method for selection of antimicrobial peptides from a phage display library by use of bacterial magnetic particles[J]. Appl Environ Microbiol, 2008,74(24):7600-7606.
[37]Wang W, Liu Y, Zu X, et al. Blocking peptides against HBV PreS1protein selected from a phage display library[J]. Biochem Biophys Res Commun, 2011, 412:633-637.
[38]Hart P, Wood TM, Tehrani K, et al. De novo identification of lipid II binding lipopeptides with antibacterial activity against vancomycinresistant bacteria[J]. Chem Sci, 2017, 8(12):7991-7997.
[39]ImjongjirakC,AmphaiphanP,CharoensapsriW,etal.Characterization and antimicrobial evaluation of SpPR-AMP1,a proline-rich antimicrobial peptide from the mud crab Scylla paramamosain[J]. Dev Comp Immunol, 2017, 74:209-216.
[40]Bamdad F, Sun X, Guan LL, et al. Preparation and characterization of antimicrobial cationized peptides from barley(Hordeum vulgare L.)proteins[J]. LWT-Food Sci Technol, 2015, 63(1):29-36.
[41]Regmi S, Choi YH, Choi YS, et al. Antimicrobial peptide isolated from Bacillus amyloliquefaciens K14 revitalizes its use in combinatorial drug therapy[J]. Folia Microbiol, 2017, 62(2):127-138.
[42]Nakatsuji T, Chen TH, Narala S, et al. Antimicrobials from human skin commensal bacteria protect against Staphylococcus aureus and are deficient in atopic dermatitis[J]. Sci Transl Med, 2017, 9:e4680.
[43]Flachbartova Z, Pulzova L, Bencurova E, et al. Inhibition of multidrug resistant listeria monocytogenes by peptides isolated from combinatorial phage display libraries[J]. Microbiol Res, 2016,188:34-41.
[44]Zhang Q, Wang Y, Ji Q, et al. Selection of antiviral peptides against mink enteritis virus using a phage display peptide library[J].Curr Microbiol, 2013, 66(4):379-384.
[45]Jaynes JM, Nagpala P, Destéfano-Beltrán L, et al. Expression of a cecropin B lytic peptide analog in transgenic tobacco confers enhanced resistance to bacterial wilt caused by Pseudomonas solanacearum[J]. Plant Sci, 1993, 89(1):43-53.
[46]Osusky M, Osuska L, Kay W, et al. Genetic modification of potato against microbial diseases:in vitro and in planta activity of a dermaseptin B1 derivative, MsrA2[J]. Theor Appl Genet, 2005,111(4):711-722.
[47]范磊,张道敬,刘振华,等.多粘类芽孢杆菌HY96-2产脂肽类抗真菌物质的研究[J].天然产物研究与开发, 2012, 24:729-735.
[48]Lee N, Park Y, Kim HW, et al. Purification and characterization of lacticin Nk34 produced by lactococcus lactis Nk34 against bovine mastitis[J]. Korean J Food Sci Ani Res, 2008, 28(4):457-462.
[49]Xiong X, Yang HS, Li L, et al. Effects of antimicrobial peptides in nursery diets on growth performance of pigs reared on five different farms[J]. Livest Sci, 2014, 167:206-210.
[50]Choi SC, Ingale SL, Kim JS, et al. Effects of dietary supplementation withanantimicrobialpeptide-P5ongrowthperformance,nutrient retention, excreta and intestinal microflora and intestinal morphology of broilers[J]. Ani Feed Sci Technol, 2013, 185(1-2):78-84.
[51]Venkitanarayanan KS, Zhao T, Doyle MP. Antibacterial effect of lactoferricin B on Escherichia coli O157-H7 in ground beef[J]. J Food Protect, 1999, 62(7):747-750.
[52]López-García B, Veyrat A, Pérez-PayáE, et al. Comparison of the activity of antifungal hexapeptides and the fungicides thiabendazole and imazalil against postharvest fungal pathogens[J]. Int J Food Microbiol, 2004, 89(2-3):163-170.
[53]李蒙,陆兆新,周翔,等.复合生物源保鲜剂对樱桃番茄绿宝石保鲜效果的影响[J].食品科学, 2013, 34(18):301-306.
[54]于继男,薛璐,鲁晓翔,等.冰温结合聚赖氨酸对贮藏期间蓝莓生理品质的变化影响[J].食品工业科技, 2015(1):334-343.
[55]薛辉,涂勇刚,熊春红,等.抗菌肽快速筛选方法的研究进展[J/OL].食品科学, 2018, http://kns. cnki. net/kcms/detail/11. 2206. TS. 20181018. 1520. 054. html.
[56]Luz C, Calpe J, Saladino F, et al. Antimicrobial packaging based on varepsilon-polylysine bioactive film for the control of mycotoxigenic fungi in vitro and in bread[J]. J Food Process Preserv, 2018, 42(1):e13370.
[57]顾康博,张道敬,罗远婵,等. Bacillomycin D发酵与纯化工艺优化及其抑菌活性初步研究[J].农药学学报, 2017, 19(4):456-473.
[58]喻红波,伍冶,潘成国.抗菌脂肽替代抗生素在断奶仔猪生产中的应用效果评价[J].饲料与畜牧, 2012(9):12-14.
[59]Molinos AC, Abriouel H, Omar NB, et al. Inactivation of listeria monocytogenes in raw fruits by enterocin as-48[J]. J Food Protect, 2008, 71(12):2460-2467.
[60]黄现青,别小妹,吕凤霞,等.枯草芽孢杆菌fmbJ产脂肽抑制点青霉效果及其桃防腐试验[J].农业工程学报, 2008, 24(1):263-267.
[61]王东,孙力军,王雅玲.纳豆菌抗菌肽APNT-6对凡纳滨对虾的低温保鲜效果[J].水产学报, 2012, 36(7):1133-1139.
[62]孟攀攀,陆兆新,吕凤霞,等. Bacillomycin高产菌株的紫外诱变选育及其防治大米黄曲霉霉变的研究[J].食品工业科技,2015, 36(19):186-201.
[63]高兆建,樊陈,鞠民友.枯草芽孢杆菌抗菌肽在食品防腐中的应用性研究[J].徐州工程学院学报:自然科学版, 2013, 28(2):67-72.
[64]李德远,徐现波,熊亮.天然食品保鲜防腐剂林蛙皮抗菌肽[J].食品科学, 2002, 23(8):279-282.
[65]Neetoo H, Ye M, Chen H. Potential antimicrobials to control Listeria monocytogenes in vacuum-packaged cold-smoked salmon pate and fillets[J]. Int J Food Microbiol, 2008, 123(3):220-227.
[66]Santiago-Silva P, Soares NF, Nóbrega JE, et al. Antimicrobial efficiency of film incorporated with pediocin on preservation of sliced ham[J]. Food Control, 2009, 20(1):85-89.
[67]Zhang J, Liu G, Li P, et al. Pentocin 31-1, a novel meat-borne bacteriocin and its application as biopreservative in chill-stored tray-packaged pork meat[J]. Food Control, 2010, 21(2):198-202.
[68]Chang JY, Chang HC. Growth inhibition of foodborne pathogens by kimchi prepared with bacteriocin producing starter culture[J]. J Food Sci, 2011, 76(1):72-78.
[69]Ba?os A, García-López JD, Nú?ez C, et al. Biocontrol of Listeria monocytogenes in fish by enterocin AS-48 and Listeria lytic bacteriophage P100[J]. LWT-Food Sci Technol, 2016, 66:672-677.
[70]龚非力.医学免疫学[M].北京:科学出版社, 2003.
[71]Hanoux V, Wijkhuisen A, Alexandrenne C, et al. Polyclonal antiidiotypic antibodies which mimic an epitope of the human prion protein[J]. Mol Immunol, 2009, 46(6):1076-1083.
[72]Xu J, Zhu XJ, Li YH, et al. Expression, characterization and therapeutic efficacy of chimeric Fab of anti-idiotypic antibody NP30 against Schistosoma japonicum[J]. Acta Trop, 2011, 118(2):159-164.
[73]刘贤金,徐重新,张霄,等.一种人源抗虫基因及其编码的抗Cry1B毒素独特型单链抗体与应用:中国, 201410037175.X[P]. 2015-11-18.
[74]刘贤金,徐重新,张霄,等.一种人源抗虫基因及其编码的抗Cry1C毒素独特型单链抗体与应用:中国, 201410037240.9[P]. 2015-11-18.
[75]刘贤金,徐重新,张霄,等.一种人源抗虫基因及其编码的抗Cry1Ab毒素独特型单链抗体与应用:中国, 201410037000.9[P]. 2015-11-18.
[76]Zhang J, Valianou M, Simmons H, et al. Identification of inhibitory scFv antibodies targeting fibroblast activation protein utilizing phage display functional screens[J]. FASEB J, 2013, 27(2):581-589.
[77]Schier R, Mccall A, Adams GP, et al. Isolation of picomolar affinity anti-c-erbB-2 single-chain Fv by molecular evolution of the complementarity determining regions in the center of the antibody binding site[J]. Mol Bio, 1996, 263(4):551-567.
[78]Barderas R, Desmet J, Timmerman P, et al. Affinity maturation of antibodies assisted byin silicomodeling[J]. PNAS, 2008, 105(26):9029-9034.
[2]MembréJM, Guillou S. Latest developments in foodborne pathogen risk assessment[J]. Curr Opin Food Sci, 2016(8):120-126.
[3]Courvalin P. Why is antibiotic resistance a deadly emerging disease?[J]. Clin Microbiol Infect, 2016, 22(5):405-407.
[4]Yoo SM, Lee SY. Optical biosensors for the detection of pathogenic microorganisms[J]. Trends Biotechnol, 2016, 34(1):7-25.
[5]Costa JP, Cova M, Ferreira R, et al. Antimicrobial peptides:an alternative for innovative medicines?[J]. Appl Microbiol Biotechnol, 2015, 99(5):2023-2040.
[6]Wang G, Mishra B, Lau K, et al. Antimicrobial peptides in2014[J]. Pharmaceuticals, 2015, 8(1):123-150.
[7]Hilpert K, Hancock RE. Use of luminescent bacteria for rapid screening and characterization of short cationic antimicrobial peptidessynthesizedoncelluloseusingpeptidearray technology[J]. Nat Protoc, 2007, 2(7):1652-1660.
[8]Castel G, Chteoui M, Heyd B, et al. Phage display of combinatorial peptide libraries:application to antiviral research[J]. Molecules,2011, 16(5):3499-3518.
[9]Tucker AT, Leonard SP, DuBois CD, et al. Discovery of nextgeneration antimicrobials through bacterial self-screening of surfacedisplayed peptide libraries[J]. Cell, 2018, 172(3):618-628.
[10]Li D, Butot S, Zuber S, et al. Monitoring of foodborne viruses in berries and considerations on the use of RT-PCR methods in surveillance[J]. Food Control, 2018, 89:235-240.
[11]Jones TH, Muehlhauser V. Frequency of hepatitis E virus,rotavirus and porcine enteric calicivirus at various stages of pork carcass processing in two pork processing plants[J]. Int J Food Microbiol, 2017, 259:29-34.
[12]Zhang Y, Liu X, Wang Y, et al. Antibacterial activity and mechanism of cinnamon essential oil against Escherichia coli and Staphylococcus aureus[J]. Food Control, 2016, 59:282-289.
[13]Kirk MD, Pires SM, Black RE, et al. World health organization estimates of the global and regional disease burden of 22 foodborne bacterial, protozoal and viral diseases[J]. PLoS Med, 2015, 12(12):e1001921.
[14]Raiola A, Tenore GC, Manyes L, et al. Risk analysis of main mycotoxins occurring in food for children:an overview[J]. Food Chem Toxicol, 2015, 84:169-180.
[15]Frisvad JC. A critical review of producers of small lactone mycotoxins:patulin, penicillic acid and moniliformin[J]. World Mycotoxin J, 2018, 11(1):73-100.
[16]LuoY,LiuX,LiJ.Updatingtechniquesoncontrolling mycotoxins-a review[J]. Food Control, 2018, 89:123-132.
[17]Stiff RE, Davies AP, Mason BW, et al. Long-term health effects after resolution of acute cryptosporidium parvum infection:a 1-year follow-up of outbreak-associated cases[J]. J Med Microbiol,2017, 66(11):1607-1611.
[18]Waldram A, Vivancos R, Hartley C, et al. Prevalence of giardia infection in households of giardia cases and risk factors for household transmission[J]. BMC Infect Dis, 2017, 17(1):e486.
[19]Rostami A, Khazan H, Kia EB, et al. Molecular identification of Trichinella spp. in wild boar and serological survey of high-risk populations in Iran[J]. Food Control, 2018, 90:40-47.
[20]Ssemanda JN, Reij MW, Middendorp G, et al. Foodborne pathogens and their risk exposure factors associated with farm vegetables in Rwanda[J]. Food Control, 2018, 89:86-96.
[21]Chung PY, Khanum R. Antimicrobial peptides as potential antibiofilm agents against multidrug-resistant bacteria[J]. J Microbiol Immunol Infect, 2017, 50(4):405-410.
[22]Huang JX, Bishop-Hurley SL, Cooper MA. Development of antiinfectives using phage display:biological agents against bacteria,viruses and parasites[J]. Antimicrob Agents Ch, 2012, 56(9):4569-4582.
[23]Murakami M, Dorschner RA, Stern LJ, et al. Expression and secretion of cathelicidin antimicrobial peptides in murine mammary glands and human milk[J]. Pediatr Res, 2005, 57(1):10-15.
[24]Gao W, Xing L, Qu P, et al. Identification of a novel cathelicidin antimicrobial peptide from ducks and determination of its functional activity and antibacterial mechanism[J]. Sci Rep, 2015, 5:e17260.
[25]Li T, Wang X, Wang Y, et al. Characterization of antimicrobial peptides isolated from the processing by products of african catfish clarias gariepinus[J]. Int J Pept Res Ther, 2016, 23(2):227-233.
[26]Nayak T, Mandal SM, Neog K, et al. Characterization of a gloverinlike antimicrobial peptide isolated from muga silkworm, antheraea assamensis[J]. Int J Pept Res Ther, 2018, 24(4):337-346.
[27]LeeH,HwangJS,Lee J,etal.Scolopendin2,a cationic antimicrobial peptide from centipede, and its membrane-active mechanism[J]. Biochim Biophys Acta, 2015, 1848(2):634-642.
[28]Memarpoor-Yazdi M, Zare-Zardini H, Asoodeh A. A novel antimicrobialpeptidederivedfromtheinsectpaederus dermatitis[J]. Int J Pept Res Ther, 2012, 19(2):99-108.
[29]Tang W, Yuan H, Zhang H, et al. An antimicrobial peptide screened from casein hydrolyzate by Saccharomyces cerevisiae cell membrane affinity method[J]. Food Control, 2015, 50:413-422.
[30]Pei J, Jiang L. Antimicrobial peptide from mucus of Andrias davidianus:screening and purification by magnetic cell membrane separation technique[J]. Int J Antimicrob Agents, 2017, 50(1):41-46.
[31]TangW,ZhangH,WangL,etal.Targetedseparationof antibacterial peptide from protein hydrolysate of anchovy cooking wastewater by equilibrium dialysis[J]. Food Chem, 2015, 168:115-123.
[32]Pini A, Giuliani A, Falciani C, et al. Antimicrobial activity of novel dendrimeric peptides obtained by phage display selection and rational modification[J]. Antimicrob. Agents Ch, 2005, 49(7):2665-2672.
[33]Rao SS, Mohan KV, Atreya CD. A peptide derived from phage display library exhibits antibacterial activity against E. coli and Pseudomonas aeruginosa[J]. PLoS One, 2013, 8(2):e56081.
[34]Bishop-Hurley SL, Rea PJ, McSweeney CS. Phage-displayed peptidesselectedforbindingtocampylobacterjejuniare antimicrobial[J]. Protein Eng Des Sel, 2010, 23(10):751-757.
[35]Zoeiby AE. Identification of novel inhibitors of Pseudomonas aeruginosa MurC enzyme derived from phage-displayed peptide libraries[J]. J Antimicrob Chemoth, 2003, 51(3):531-543.
[36]Tanaka T, Kokuryu Y, Matsunaga T. Novel method for selection of antimicrobial peptides from a phage display library by use of bacterial magnetic particles[J]. Appl Environ Microbiol, 2008,74(24):7600-7606.
[37]Wang W, Liu Y, Zu X, et al. Blocking peptides against HBV PreS1protein selected from a phage display library[J]. Biochem Biophys Res Commun, 2011, 412:633-637.
[38]Hart P, Wood TM, Tehrani K, et al. De novo identification of lipid II binding lipopeptides with antibacterial activity against vancomycinresistant bacteria[J]. Chem Sci, 2017, 8(12):7991-7997.
[39]ImjongjirakC,AmphaiphanP,CharoensapsriW,etal.Characterization and antimicrobial evaluation of SpPR-AMP1,a proline-rich antimicrobial peptide from the mud crab Scylla paramamosain[J]. Dev Comp Immunol, 2017, 74:209-216.
[40]Bamdad F, Sun X, Guan LL, et al. Preparation and characterization of antimicrobial cationized peptides from barley(Hordeum vulgare L.)proteins[J]. LWT-Food Sci Technol, 2015, 63(1):29-36.
[41]Regmi S, Choi YH, Choi YS, et al. Antimicrobial peptide isolated from Bacillus amyloliquefaciens K14 revitalizes its use in combinatorial drug therapy[J]. Folia Microbiol, 2017, 62(2):127-138.
[42]Nakatsuji T, Chen TH, Narala S, et al. Antimicrobials from human skin commensal bacteria protect against Staphylococcus aureus and are deficient in atopic dermatitis[J]. Sci Transl Med, 2017, 9:e4680.
[43]Flachbartova Z, Pulzova L, Bencurova E, et al. Inhibition of multidrug resistant listeria monocytogenes by peptides isolated from combinatorial phage display libraries[J]. Microbiol Res, 2016,188:34-41.
[44]Zhang Q, Wang Y, Ji Q, et al. Selection of antiviral peptides against mink enteritis virus using a phage display peptide library[J].Curr Microbiol, 2013, 66(4):379-384.
[45]Jaynes JM, Nagpala P, Destéfano-Beltrán L, et al. Expression of a cecropin B lytic peptide analog in transgenic tobacco confers enhanced resistance to bacterial wilt caused by Pseudomonas solanacearum[J]. Plant Sci, 1993, 89(1):43-53.
[46]Osusky M, Osuska L, Kay W, et al. Genetic modification of potato against microbial diseases:in vitro and in planta activity of a dermaseptin B1 derivative, MsrA2[J]. Theor Appl Genet, 2005,111(4):711-722.
[47]范磊,张道敬,刘振华,等.多粘类芽孢杆菌HY96-2产脂肽类抗真菌物质的研究[J].天然产物研究与开发, 2012, 24:729-735.
[48]Lee N, Park Y, Kim HW, et al. Purification and characterization of lacticin Nk34 produced by lactococcus lactis Nk34 against bovine mastitis[J]. Korean J Food Sci Ani Res, 2008, 28(4):457-462.
[49]Xiong X, Yang HS, Li L, et al. Effects of antimicrobial peptides in nursery diets on growth performance of pigs reared on five different farms[J]. Livest Sci, 2014, 167:206-210.
[50]Choi SC, Ingale SL, Kim JS, et al. Effects of dietary supplementation withanantimicrobialpeptide-P5ongrowthperformance,nutrient retention, excreta and intestinal microflora and intestinal morphology of broilers[J]. Ani Feed Sci Technol, 2013, 185(1-2):78-84.
[51]Venkitanarayanan KS, Zhao T, Doyle MP. Antibacterial effect of lactoferricin B on Escherichia coli O157-H7 in ground beef[J]. J Food Protect, 1999, 62(7):747-750.
[52]López-García B, Veyrat A, Pérez-PayáE, et al. Comparison of the activity of antifungal hexapeptides and the fungicides thiabendazole and imazalil against postharvest fungal pathogens[J]. Int J Food Microbiol, 2004, 89(2-3):163-170.
[53]李蒙,陆兆新,周翔,等.复合生物源保鲜剂对樱桃番茄绿宝石保鲜效果的影响[J].食品科学, 2013, 34(18):301-306.
[54]于继男,薛璐,鲁晓翔,等.冰温结合聚赖氨酸对贮藏期间蓝莓生理品质的变化影响[J].食品工业科技, 2015(1):334-343.
[55]薛辉,涂勇刚,熊春红,等.抗菌肽快速筛选方法的研究进展[J/OL].食品科学, 2018, http://kns. cnki. net/kcms/detail/11. 2206. TS. 20181018. 1520. 054. html.
[56]Luz C, Calpe J, Saladino F, et al. Antimicrobial packaging based on varepsilon-polylysine bioactive film for the control of mycotoxigenic fungi in vitro and in bread[J]. J Food Process Preserv, 2018, 42(1):e13370.
[57]顾康博,张道敬,罗远婵,等. Bacillomycin D发酵与纯化工艺优化及其抑菌活性初步研究[J].农药学学报, 2017, 19(4):456-473.
[58]喻红波,伍冶,潘成国.抗菌脂肽替代抗生素在断奶仔猪生产中的应用效果评价[J].饲料与畜牧, 2012(9):12-14.
[59]Molinos AC, Abriouel H, Omar NB, et al. Inactivation of listeria monocytogenes in raw fruits by enterocin as-48[J]. J Food Protect, 2008, 71(12):2460-2467.
[60]黄现青,别小妹,吕凤霞,等.枯草芽孢杆菌fmbJ产脂肽抑制点青霉效果及其桃防腐试验[J].农业工程学报, 2008, 24(1):263-267.
[61]王东,孙力军,王雅玲.纳豆菌抗菌肽APNT-6对凡纳滨对虾的低温保鲜效果[J].水产学报, 2012, 36(7):1133-1139.
[62]孟攀攀,陆兆新,吕凤霞,等. Bacillomycin高产菌株的紫外诱变选育及其防治大米黄曲霉霉变的研究[J].食品工业科技,2015, 36(19):186-201.
[63]高兆建,樊陈,鞠民友.枯草芽孢杆菌抗菌肽在食品防腐中的应用性研究[J].徐州工程学院学报:自然科学版, 2013, 28(2):67-72.
[64]李德远,徐现波,熊亮.天然食品保鲜防腐剂林蛙皮抗菌肽[J].食品科学, 2002, 23(8):279-282.
[65]Neetoo H, Ye M, Chen H. Potential antimicrobials to control Listeria monocytogenes in vacuum-packaged cold-smoked salmon pate and fillets[J]. Int J Food Microbiol, 2008, 123(3):220-227.
[66]Santiago-Silva P, Soares NF, Nóbrega JE, et al. Antimicrobial efficiency of film incorporated with pediocin on preservation of sliced ham[J]. Food Control, 2009, 20(1):85-89.
[67]Zhang J, Liu G, Li P, et al. Pentocin 31-1, a novel meat-borne bacteriocin and its application as biopreservative in chill-stored tray-packaged pork meat[J]. Food Control, 2010, 21(2):198-202.
[68]Chang JY, Chang HC. Growth inhibition of foodborne pathogens by kimchi prepared with bacteriocin producing starter culture[J]. J Food Sci, 2011, 76(1):72-78.
[69]Ba?os A, García-López JD, Nú?ez C, et al. Biocontrol of Listeria monocytogenes in fish by enterocin AS-48 and Listeria lytic bacteriophage P100[J]. LWT-Food Sci Technol, 2016, 66:672-677.
[70]龚非力.医学免疫学[M].北京:科学出版社, 2003.
[71]Hanoux V, Wijkhuisen A, Alexandrenne C, et al. Polyclonal antiidiotypic antibodies which mimic an epitope of the human prion protein[J]. Mol Immunol, 2009, 46(6):1076-1083.
[72]Xu J, Zhu XJ, Li YH, et al. Expression, characterization and therapeutic efficacy of chimeric Fab of anti-idiotypic antibody NP30 against Schistosoma japonicum[J]. Acta Trop, 2011, 118(2):159-164.
[73]刘贤金,徐重新,张霄,等.一种人源抗虫基因及其编码的抗Cry1B毒素独特型单链抗体与应用:中国, 201410037175.X[P]. 2015-11-18.
[74]刘贤金,徐重新,张霄,等.一种人源抗虫基因及其编码的抗Cry1C毒素独特型单链抗体与应用:中国, 201410037240.9[P]. 2015-11-18.
[75]刘贤金,徐重新,张霄,等.一种人源抗虫基因及其编码的抗Cry1Ab毒素独特型单链抗体与应用:中国, 201410037000.9[P]. 2015-11-18.
[76]Zhang J, Valianou M, Simmons H, et al. Identification of inhibitory scFv antibodies targeting fibroblast activation protein utilizing phage display functional screens[J]. FASEB J, 2013, 27(2):581-589.
[77]Schier R, Mccall A, Adams GP, et al. Isolation of picomolar affinity anti-c-erbB-2 single-chain Fv by molecular evolution of the complementarity determining regions in the center of the antibody binding site[J]. Mol Bio, 1996, 263(4):551-567.
[78]Barderas R, Desmet J, Timmerman P, et al. Affinity maturation of antibodies assisted byin silicomodeling[J]. PNAS, 2008, 105(26):9029-9034.