加减八珍汤对人鼻咽癌6-10B裸鼠移植瘤生长抑制作用及瘤体组织中cyclinD1表达水平影响
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摘要
目的:构建人鼻咽癌6-10B裸鼠移植瘤模型,给予该模型鼠加减八珍汤处理,通过HE染色、RT-RCR及Western blot,探讨该方对鼻咽癌裸鼠移植瘤生长及增殖能力的影响。方法:将10只建模成功的鼻咽癌裸鼠随机分为实验组和对照组,分别给予加减八珍汤(浓度150 mg/mL)和0.9%生理盐水各0.2 mL灌胃,每天两次,共14 d。观察裸鼠瘤体积的变化至第21天,显微镜观察移植瘤组织病理变化特点,RT-RCR及Western blot检测移植瘤组织中cyclinD1的表达。结果:HE染色证实人鼻咽癌6-10B裸鼠移植瘤建模成功。RT-PCR和Western blot检测结果表明加减八珍汤可显著下调cyclinD1 mRNA和蛋白质的表达(P<0.05),抑制癌细胞的增殖,延缓肿瘤的生长。结论:在裸鼠体内,加减八珍汤能抑制人鼻咽癌细胞6-10B的增殖,从而达到抑制鼻咽癌组织生长的作用。
Objective:To construct a human nasopharyngeal carcinoma 6-10 B nude mice xenograft model and to treat the model rats with addition and subtraction of Bazhen Decoction. The HE staining,RTRCR and Western blot were used to investigate the effects and mechanism on the tumor tissue in nude mice. Methods:Ten nude mice with successful modeling of nasopharyngeal carcinoma were randomly divided into experimental group and control group. They were given addition and subtraction of Bazhen Decoction(concentration 150 mg/mL)or 0.9% normal saline 0.2 mL each twice a day and 14 days in total. The tumor volumes of nude mice were observed until the 21 st day. The pathological changes of the transplanted tumor were observed under microscope. The expression of cyclinD1 was detected by RT-RCR and Western blot. Results:HE staining confirmed the successful modeling of human nasopharyngeal carcinoma 6-10 B nude mice. The results of RT-PCR and Western blotting showed that the decoction could significantly down-regulate the expression of cyclinD1(P<0.05),inhibit the proliferation of cancer cells and delay the growth of tumors. Conclusion:In nude mice,addition and subtraction of Bazhen Decoction can inhibit the proliferation of human nasopharyngeal carcinoma cell line 6-10 B,thereby inhibiting the growth of nasopharyngeal carcinoma.
Objective:To construct a human nasopharyngeal carcinoma 6-10 B nude mice xenograft model and to treat the model rats with addition and subtraction of Bazhen Decoction. The HE staining,RTRCR and Western blot were used to investigate the effects and mechanism on the tumor tissue in nude mice. Methods:Ten nude mice with successful modeling of nasopharyngeal carcinoma were randomly divided into experimental group and control group. They were given addition and subtraction of Bazhen Decoction(concentration 150 mg/mL)or 0.9% normal saline 0.2 mL each twice a day and 14 days in total. The tumor volumes of nude mice were observed until the 21 st day. The pathological changes of the transplanted tumor were observed under microscope. The expression of cyclinD1 was detected by RT-RCR and Western blot. Results:HE staining confirmed the successful modeling of human nasopharyngeal carcinoma 6-10 B nude mice. The results of RT-PCR and Western blotting showed that the decoction could significantly down-regulate the expression of cyclinD1(P<0.05),inhibit the proliferation of cancer cells and delay the growth of tumors. Conclusion:In nude mice,addition and subtraction of Bazhen Decoction can inhibit the proliferation of human nasopharyngeal carcinoma cell line 6-10 B,thereby inhibiting the growth of nasopharyngeal carcinoma.
引文
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[2]SUN Y,LI WF,CHEN NY,et al.Induction chemotherapy plus concurrent chemoradiotherapy versus concurrent chemoradiotherapy alone in locoregionally advanced nasopharyngeal carcinoma:a phase 3,multicentre,randomised controlled trial[J].Lancet Oncol,2016,17(11):1509-1520.
[3]WANG M,TIAN H,LI G,et al.Significant benefits of adding neoadjuvant chemotherapy before concurrent chemoradiotherapy for locoregionally advanced nasopharyngeal carcinoma:a metaanalysis of randomized controlled trials[J].Oncotarget,2016,7(30):48375-48390.
[4]Cho W C,Chen H Y.Clinical efficacy of traditional Chinese medicine as a concomitant therapy for nasopharyngeal carcinoma:a systematic review and meta-analysis[J].Cancer Investigation,2009,27(3):334-344.
[5]LU MQ,KONG QZ,XU XH,et al.Pectolinarigenin,a flavonoid compound from cirsium japonicum with potential antiproliferation activity in MCF-7 breast cancer cell[J].Trop J Pharm Res,2014,13(2):225-228.
[6]董建新,江春花,王艳.加减八珍汤联合放化疗对中晚期宫颈癌患者疗效及对生存期影响[J].辽宁中医药大学学报,2017,19(8):128-130.
[7]张珺珺,忻耀杰,滕磊,等.鼻内镜手术结合“加减八珍汤”治疗慢性鼻-鼻窦炎53例临床观察[J].江苏中医药,2012,44(8):41-42.
[8]霍旋,冯群虎.加减八珍汤治疗女性虚证便秘50例疗效观察[J].山东中医杂志,2016(4):302-304.
[9]王家元.加减八珍湯治疗11例泛发性神经性皮炎[J].四川医学,1981(6):657.
[10]王士贞.中医耳鼻咽喉科学[M].北京:中国中医药出版社,2003:214-215.
[11]Lee MH,Yang HY.Regulators of G1 cyclin-dependent kinases and cancers[J].Cancer Metastasis Rev,2003,22(4):435-449.
[12]Deshpande A,Sicinski P,Hinds P W.Cyclins and cdks in development and cancer:a perspective[J].Oncogene,2005,24(17):2909-2915.
[13]Kim J K,Diehl J A.Nuclear cyclin D1:An oncogenic driver in human cancer[J].Journal of Cellular Physiology,2010,220(2):292-296.
[14]Musgrove E A,Caldon C E,Barraclough J,et al.Cyclin D as a therapeutic target in cancer[J].Nature Reviews Cancer,2011,11(8):558-572.
[15]Chou J,Lin Y C,Kim J,et al.Nasopharyngeal carcinoma--review of the molecular mechanisms of tumorigenesis[J].Head&Neck,2010,30(7):946-963.
[16]HUANG XM,DAI CB,MOU ZL,et al.Overproduction of Cyclin D1 is dependent on activated mTORC1 signal in nasopharyngeal carcinoma:Implication for therapy[J].Cancer Letters,2009,279(1):56.