香栓菌的平喘及抗氧化活性研究
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摘要
本文对香栓菌子实体提取物的平喘作用和抗氧化活性进行研究。平喘药理实验采用鸡卵清蛋白(OVA)致敏法建立小鼠过敏性哮喘模型,灌胃给药香栓菌水提物(高240mg/kg、中120mg/kg、低60mg/kg剂量)和石油醚提取物(高24mg/kg、中12mg/kg、低6mg/kg剂量)。比较各组小鼠行为变化,检测IgE、TNF-α、IL-1β、IL-6、IL-4和IFN-γ等指标,分类计数血液及支气管肺泡灌洗液(BALF)中的嗜酸性粒细胞数量,分析肺组织病理变化;抗氧化实验采用香栓菌水提取物、乙酸乙酯提取物、氯仿提取物、石油醚提取物比较清除DPPH和ABTS自由基能力。平喘试验结果表明,香栓菌水提物中剂量(120mg/kg)能降低血中嗜酸性粒细胞(Eos)及血清中IgE含量,减少BALF中Eos,降低血清及肺组织匀浆中IL-4含量,提高IFN-γ/IL-4,减轻模型的病理改变;石油醚提取物高剂量(24mg/kg)能降低血中Eos和血清中IgE、TNF-α、IL-1、IL-6含量,减少肺组织炎症细胞浸润;抗氧化结果显示,香栓菌各层提取物均有不同程度的抗氧化能力,并呈现一定的量效关系,同时水提取物的抗氧化效果最显著。本文为深入研究香栓菌子实体对呼吸系统疾病的治疗提供了重要试验依据。
Treatment effect for OVA-induced allergic asthma and antioxidant activities of Trametes suaveolens fruiting body extract were investigated. Mice injected with OVA were used for establishment of allergic asthma model. Different dosage of the fruit body water extract(TSWE) and petroleum ether extract(TSPEE) wereadministrated to the mice. The morphological changes of lung tissue were observed, and the inflammatory cells in bronchoalveolar lavage fluid(BALF) were examined. Inflammatory cytokines(IgE, TNF-α, IL-1β, IL-6, IL-4, IFN-γ) in serum and lung homogenates were determined for each experimental group. The antioxidant activities of different polarity extract of the fruiting bodies were evaluated by determining scavenging rates against DPPH and ABTS free radicals. The result showed that TSWE moderate dose(120 mg/kg) displayed a decrease of Eos in blood and BALF and IgE content in serum, and could reduce IL-4 and increase IFN-γ/IL-4 in serum and lung homogenate. TSPEE high dose(24 mg/kg) displayed a decrease of Eos in blood and IgE, TNF-α, IL-1, IL-6 content in serum, and reduced lung tissue inflammatory cell infiltration. The results indicated that petroleum ether extract, chloroform extract, ethyl acetate extract and water extract of Trametes suaveolens fruiting bodies showed antioxidant capacity to different extent, presenting relation between dosage and curative effect. The antioxidant effect of water extract was remarkable. This study provides reference for treatment of respiratory system diseases by administrating Trametes suaveolens fruiting body extract.
Treatment effect for OVA-induced allergic asthma and antioxidant activities of Trametes suaveolens fruiting body extract were investigated. Mice injected with OVA were used for establishment of allergic asthma model. Different dosage of the fruit body water extract(TSWE) and petroleum ether extract(TSPEE) wereadministrated to the mice. The morphological changes of lung tissue were observed, and the inflammatory cells in bronchoalveolar lavage fluid(BALF) were examined. Inflammatory cytokines(IgE, TNF-α, IL-1β, IL-6, IL-4, IFN-γ) in serum and lung homogenates were determined for each experimental group. The antioxidant activities of different polarity extract of the fruiting bodies were evaluated by determining scavenging rates against DPPH and ABTS free radicals. The result showed that TSWE moderate dose(120 mg/kg) displayed a decrease of Eos in blood and BALF and IgE content in serum, and could reduce IL-4 and increase IFN-γ/IL-4 in serum and lung homogenate. TSPEE high dose(24 mg/kg) displayed a decrease of Eos in blood and IgE, TNF-α, IL-1, IL-6 content in serum, and reduced lung tissue inflammatory cell infiltration. The results indicated that petroleum ether extract, chloroform extract, ethyl acetate extract and water extract of Trametes suaveolens fruiting bodies showed antioxidant capacity to different extent, presenting relation between dosage and curative effect. The antioxidant effect of water extract was remarkable. This study provides reference for treatment of respiratory system diseases by administrating Trametes suaveolens fruiting body extract.
引文
Dai YC,2010.Species diversity of wood-decaying fungi in Northeast China.Mycosystema,29(6):801-818(in Chinese)
Dai YC,2012.Pathogenic wood-decaying fungi on woody plants in China.Mycosystema,31(4):493-509(in Chinese)
Huang HR,Zhong YQ,Wu JF,2012.The association between IFN-γand IL-4 genetic polymorphisms and childhood susceptibility to bronchial asthma.Gene,494(1):96-101
Li D,Zhang B,Li Y,2013.Screening of high laccase-producing strains and studies on degradation of naphthalene in sewage.Journal of Anhui Agricultural Sciences,41(22):9179-9182+9185(in Chinese)
Li DW,Long K,Su MS,Liang YH,Xie XM,2018.Active component extracted from Inonotus obliquus mycoplasm and its anti-oxidation activity in vitro.Journal of Fungal Research,16(1):51-56(in Chinese)
Li SZ,2004.Compendium of materia medica in Ming dynasty.People′s Medical Publishing House,Beijing.1708-1712(in Chinese)
Li W,Bao HY,Bau T,2014.Chemical constituents and antioxidant activities of Laetiporus cremeiporus.Mycosystema,33(2):365-374(in Chinese)
Li Y,Li TH,Yang ZL,Bau T,Dai YC,2015.Resource mapping of large fungi in China.Zhong Yuan Farmers Press,Zhengzhou.1-663(in Chinese)
Kenji O,Koji I,Yuya A,Asako O,Motoki Y,Yasuyuki N,Nitaro M,Hideshi Y,2010.Production of ethanol by the white-rot basidiomycetes Peniophora cinerea and Trametes suaveolens.Biotechnology Letters,32(7):909-913
Piao CH,Bui TT,Song CH,Shin HS,Shon DH,Chai OH,2017.Trigonella foenum-graecum alleviates airway inflammation of allergic asthma in ovalbumin-induced mouse model.Biochemical&Biophysical Research Communications,482(4):1284
Tang SW,2011.Syndrome materia medica in Northern Song dynasty.China Medical Science Press,Beijing.187-188(in Chinese)
Wang HZ,Hong M,Gui LL,Hua YQ,Xu HQ,2013.Effect of Yupingfeng San against OVA-induced allergic asthma in mice.China Journal of Chinese Materia Medica,38(7):1052-1055(in Chinese)
Wang J,Wang HX,Ng TB,2007.A peptide with HIV-1 reverse transcriptase inhibitory activity from the medicinal mushroom Russula paludosa.Peptides,28(3):560-565
Weng FY,Lin JQ,Wang CZ,Guo JL,Lin ZX,Liu WH,2018.Effects of ethanol extract of Taiwanofungus camphoratus fruiting body on the OVA-induced allergic pneumonia.Mycosystema,37(8):1063-1069(in Chinese)
Xu J,Jin XJ,Miao ZH,Xia B,Dai YR,2018.Th17 lymphocyte and inflammation-related cytokine levels in mice with bronchial asthma.Zhejiang Medical Journal,40(3):221-223+230(in Chinese)
Ye P,Yang XL,Chen X,Shi C,2017.Hyperoside attenuates ova-induced allergic airway inflammation by activating nrf2.International Immunopharmacology,44:168-173
Yuan J,Hong YZ,Zhang XQ,Xiao YZ,2007.Separation and identification of one new lignin peroxidase-producing Trametes strain.Letters in Biotechnology,18(6):949-951(in Chinese)
Zhao Y,Bao HY,Bau T,Li Y,2016.Textural research of“Baizhi”.Journal of Fungal Research,16(1):57-62(in Chinese)
Zhao Y,Bao HY,Yan J,Yang S,Bau T,Li Y,2018.Effects of relieving cough and sputum and anti-hypoxia activities of Trametes suaveolens extract.Mycosystema,37(8):1-12(in Chinese)
Zhuang MY,1992.Study on polysaccharide from Trametes fragilis.Natural Product Research and Development,4(4):8-12(in Chinese)
戴玉成,2010.中国东北地区木材腐朽菌的多样性.菌物学报,29(6):801-818
戴玉成,2012.中国木本植物病原木材腐朽菌研究.菌物学报,31(4):493-509
李丹,张波,李玉,2013.高产漆酶菌的筛选及对污水中萘降解的研究.安徽农业科学,41(22):9179-9182+9185
李东文,龙凯,苏明声,梁永红,谢小梅,2018.桦褐孔菌菌质活性组分的提取及其体外抗氧化作用.菌物研究,16(1):51-56
李时珍,2004.明代本草纲目菜部.北京:人民卫生出版社.1708-1712
李玉,李泰辉,杨祝良,图力古尔,戴玉成,2015.中国大型菌物资源图鉴.郑州:中原农民出版社.663
李巍,包海鹰,图力古尔,2014.乳孔硫磺菌的化学成分和抗氧化活性.菌物学报,33(2):365-374
唐慎微,2011.北宋证类本草.北京:中国医药科技出版社.187-188
王慧珠,洪敏,桂黎黎,华永庆,许惠琴,2013.玉屏风散抗OVA致小鼠过敏性哮喘的作用研究.中国中药杂志,38(7):1052-1055
翁丰岳,林佳祺,王承中,郭建良,林仲相,刘文煌,2018.牛樟芝子实体醇提物对过敏性肺炎的作用.菌物学报,37(8):1063-1069
徐静,金晓洁,缪志海,夏斌,戴元荣,2018.支气管哮喘小鼠细胞免疫因子及Th(17)细胞水平的变化及发病机制研究.浙江医学,40(3):221-223+230
袁璟,洪宇植,张小青,肖亚中,2007.一株新的木素过氧化物酶产生菌的分离和鉴定.生物技术通讯,18(6):949-951
赵宇,包海鹰,图力古尔,李玉,2016.白芝的本草考证.菌物研究,16(1):57-62
赵宇,包海鹰,闫俊,杨烁,图力古尔,李玉,2018.香栓菌提取物的止咳化痰作用及耐缺氧药理活性研究.菌物学报,37(8):1-12
庄名扬,1992.香栓菌多糖的研究.天然产物研究与开发,4(4):8-12
Dai YC,2012.Pathogenic wood-decaying fungi on woody plants in China.Mycosystema,31(4):493-509(in Chinese)
Huang HR,Zhong YQ,Wu JF,2012.The association between IFN-γand IL-4 genetic polymorphisms and childhood susceptibility to bronchial asthma.Gene,494(1):96-101
Li D,Zhang B,Li Y,2013.Screening of high laccase-producing strains and studies on degradation of naphthalene in sewage.Journal of Anhui Agricultural Sciences,41(22):9179-9182+9185(in Chinese)
Li DW,Long K,Su MS,Liang YH,Xie XM,2018.Active component extracted from Inonotus obliquus mycoplasm and its anti-oxidation activity in vitro.Journal of Fungal Research,16(1):51-56(in Chinese)
Li SZ,2004.Compendium of materia medica in Ming dynasty.People′s Medical Publishing House,Beijing.1708-1712(in Chinese)
Li W,Bao HY,Bau T,2014.Chemical constituents and antioxidant activities of Laetiporus cremeiporus.Mycosystema,33(2):365-374(in Chinese)
Li Y,Li TH,Yang ZL,Bau T,Dai YC,2015.Resource mapping of large fungi in China.Zhong Yuan Farmers Press,Zhengzhou.1-663(in Chinese)
Kenji O,Koji I,Yuya A,Asako O,Motoki Y,Yasuyuki N,Nitaro M,Hideshi Y,2010.Production of ethanol by the white-rot basidiomycetes Peniophora cinerea and Trametes suaveolens.Biotechnology Letters,32(7):909-913
Piao CH,Bui TT,Song CH,Shin HS,Shon DH,Chai OH,2017.Trigonella foenum-graecum alleviates airway inflammation of allergic asthma in ovalbumin-induced mouse model.Biochemical&Biophysical Research Communications,482(4):1284
Tang SW,2011.Syndrome materia medica in Northern Song dynasty.China Medical Science Press,Beijing.187-188(in Chinese)
Wang HZ,Hong M,Gui LL,Hua YQ,Xu HQ,2013.Effect of Yupingfeng San against OVA-induced allergic asthma in mice.China Journal of Chinese Materia Medica,38(7):1052-1055(in Chinese)
Wang J,Wang HX,Ng TB,2007.A peptide with HIV-1 reverse transcriptase inhibitory activity from the medicinal mushroom Russula paludosa.Peptides,28(3):560-565
Weng FY,Lin JQ,Wang CZ,Guo JL,Lin ZX,Liu WH,2018.Effects of ethanol extract of Taiwanofungus camphoratus fruiting body on the OVA-induced allergic pneumonia.Mycosystema,37(8):1063-1069(in Chinese)
Xu J,Jin XJ,Miao ZH,Xia B,Dai YR,2018.Th17 lymphocyte and inflammation-related cytokine levels in mice with bronchial asthma.Zhejiang Medical Journal,40(3):221-223+230(in Chinese)
Ye P,Yang XL,Chen X,Shi C,2017.Hyperoside attenuates ova-induced allergic airway inflammation by activating nrf2.International Immunopharmacology,44:168-173
Yuan J,Hong YZ,Zhang XQ,Xiao YZ,2007.Separation and identification of one new lignin peroxidase-producing Trametes strain.Letters in Biotechnology,18(6):949-951(in Chinese)
Zhao Y,Bao HY,Bau T,Li Y,2016.Textural research of“Baizhi”.Journal of Fungal Research,16(1):57-62(in Chinese)
Zhao Y,Bao HY,Yan J,Yang S,Bau T,Li Y,2018.Effects of relieving cough and sputum and anti-hypoxia activities of Trametes suaveolens extract.Mycosystema,37(8):1-12(in Chinese)
Zhuang MY,1992.Study on polysaccharide from Trametes fragilis.Natural Product Research and Development,4(4):8-12(in Chinese)
戴玉成,2010.中国东北地区木材腐朽菌的多样性.菌物学报,29(6):801-818
戴玉成,2012.中国木本植物病原木材腐朽菌研究.菌物学报,31(4):493-509
李丹,张波,李玉,2013.高产漆酶菌的筛选及对污水中萘降解的研究.安徽农业科学,41(22):9179-9182+9185
李东文,龙凯,苏明声,梁永红,谢小梅,2018.桦褐孔菌菌质活性组分的提取及其体外抗氧化作用.菌物研究,16(1):51-56
李时珍,2004.明代本草纲目菜部.北京:人民卫生出版社.1708-1712
李玉,李泰辉,杨祝良,图力古尔,戴玉成,2015.中国大型菌物资源图鉴.郑州:中原农民出版社.663
李巍,包海鹰,图力古尔,2014.乳孔硫磺菌的化学成分和抗氧化活性.菌物学报,33(2):365-374
唐慎微,2011.北宋证类本草.北京:中国医药科技出版社.187-188
王慧珠,洪敏,桂黎黎,华永庆,许惠琴,2013.玉屏风散抗OVA致小鼠过敏性哮喘的作用研究.中国中药杂志,38(7):1052-1055
翁丰岳,林佳祺,王承中,郭建良,林仲相,刘文煌,2018.牛樟芝子实体醇提物对过敏性肺炎的作用.菌物学报,37(8):1063-1069
徐静,金晓洁,缪志海,夏斌,戴元荣,2018.支气管哮喘小鼠细胞免疫因子及Th(17)细胞水平的变化及发病机制研究.浙江医学,40(3):221-223+230
袁璟,洪宇植,张小青,肖亚中,2007.一株新的木素过氧化物酶产生菌的分离和鉴定.生物技术通讯,18(6):949-951
赵宇,包海鹰,图力古尔,李玉,2016.白芝的本草考证.菌物研究,16(1):57-62
赵宇,包海鹰,闫俊,杨烁,图力古尔,李玉,2018.香栓菌提取物的止咳化痰作用及耐缺氧药理活性研究.菌物学报,37(8):1-12
庄名扬,1992.香栓菌多糖的研究.天然产物研究与开发,4(4):8-12