UV-B辐射增强对芦苇生长及生理特性的影响
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摘要
在UV-B辐射增强条件下,以芦苇为材料,研究了不同辐射梯度对芦苇生长及生理特性的影响.结果表明:与自然光照相比,UV-B辐射增强可显著降低芦苇植株高度、比叶面积、生物量,且高强度辐射处理的影响大于低强度辐射,随辐射时间延长,生物量分配有向地下部分转移的趋势;与自然光照相比,UV-B辐射增强可显著增加芦苇叶片丙二醛(MDA)含量、抗氧化酶活性(SOD、CAT、GR、APX)、抗氧化物质含量(GSH、As A),且随辐射强度增大,MDA含量、酶活性、抗氧化物质含量增强程度加剧,但随辐射时间延长,高辐射处理下,GR酶活显著低于对照,GSH也表现出相应的趋势;UV-B辐射增强条件下,类胡萝卜素含量显著降低,而类黄酮含量较对照组却显著升高,且高强度辐射处理对芦苇叶片紫外吸收物质的影响大于低强度辐射.
The effects of UV-B radiation intensities on growth and physiological characteristics of Phragmites australis had been studied.The results showed that the enhanced UV-B radiation significantly reduced the plant height,specific leaf area and biomass,the effects of high intensity of UV-B radiation was greater than that of lower' s,and thebiomass allocation tended to shift to the underground with the lengthening of radiation time. The MDA content,antioxidant enzyme activities( SOD,CAT,GR,APX) and antioxidants( GSH,As A) increased significantly compared with the control,and the degree of intensification increased while increasing radiation intensity. However,when prolonging the radiation time,the GR activity was significantly lower under high irradiation than that under the control,and GSH showed a corresponding trend. The carotenoid content decreased and flavonoid increased significantly compared with those of the control. The effects of high intensity of radiation on UV absorbing material was greater than that of lower intensity of radiation.
The effects of UV-B radiation intensities on growth and physiological characteristics of Phragmites australis had been studied.The results showed that the enhanced UV-B radiation significantly reduced the plant height,specific leaf area and biomass,the effects of high intensity of UV-B radiation was greater than that of lower' s,and thebiomass allocation tended to shift to the underground with the lengthening of radiation time. The MDA content,antioxidant enzyme activities( SOD,CAT,GR,APX) and antioxidants( GSH,As A) increased significantly compared with the control,and the degree of intensification increased while increasing radiation intensity. However,when prolonging the radiation time,the GR activity was significantly lower under high irradiation than that under the control,and GSH showed a corresponding trend. The carotenoid content decreased and flavonoid increased significantly compared with those of the control. The effects of high intensity of radiation on UV absorbing material was greater than that of lower intensity of radiation.
引文
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Dai Q,Yan B,Huang S,et al.1997.Response of oxidative stress defense systems in rice(Oryza sativa)leaves with supplemental UV-B radiation[J].Physiology Plantarum,101(2):301-308
杜照奎,李钧敏,钟章成.2014.花生幼苗对UV-B辐射增强的生理生化响应[J].西南大学学报,36(9):50-56
Ellman G L.1959.Tissue sulthydryl groups[J].Arch Biochem Biophys,82:70-77
Galatro A,Simontacchi M,Puntarulo S.2001.Free radical generation and antioxidant content in chloroplasts from soybean leaves exposed to ultraviolet-B[J].Physiology Plantarum,113(4):564-570
高俊凤.2006.植物生理学实验指导[M].北京:高等教育出版社.74-75;88-89
Giannopolitis C N,Ries S K,Superoxide Dismutases I.1977.Occurrence in higher plants[J].Plant Physiology,59(2):309-314
韩雯,韩榕.2015.不同时间的UV-B辐射对拟南芥幼苗生长的影响[J].植物学报,50(1):40-46
何永美,湛方栋,祖艳群,等.2013.大田条件下UV-B辐射对元阳梯田2 个地方水稻品种硅、类黄酮和总酚含量的影响[J].农业环境科学学报,(8):1500-1506
祭美菊,冯虎元,安黎哲,等.2002.增强的UVB辐射对植物影响的研究[J].应用生态学报,13(3):359-364
Jordan B R.1996.The effects of ultraviolet-B radiation on plants:a molecular perspective[J].Adv Bot Res,22:97-162
Jovanovic Z S,Milosevic J D,Radovic S R.2006.Antioxidative enzymes in the response of buckwheat(Fagopyrum esculentum Moench)to ultraviolet B radiation[J].Journal of Agricultural and Food Chemistry,54(25):9472-9478
Kerri L B.1994.Evidence for large upward trends of ultraviolet-B radiation lined to ozone depletion[J].Science,262:1032-1034
Knorzer O C,Durner J,Boger P.1996.Alteration in the antioxidative system of suspension cultivated soybean cells(Clycine max)induced by oxidative stress[J].Physiologic Plantarum,97(2):388-396
Law M,Charles S A,Halliwell B.1983.Glutathione and ascorbic acid in spinach(Spinacia olenacea)chloroplasts[J].Biochemical Journal,210:899-903
李惠梅,师生波.2010.长期增强UV-B辐射对高寒矮嵩草草甸植物光合作用的影响[J].西北植物学报,30(6):1186-1196
李良博,张连根,唐天向,等.2016.UV-B辐射增强对紫茎泽兰和艾草抗性生理特性的影响[J].西北植物学报,36(2):343-352
李元,何永美,祖艳群.2006.大田增强UV-B辐射对元阳梯田地方水稻茎秆性状和倒伏指数的影响[J].应用生态学报,17(1):123-126
李元,王勋陵.1998.紫外辐射增加对春小麦生理、产量和品质的影响[J].环境科学学报,18(5):504-509
刘丽丽,张文会,范颖伦,等.2010.不同剂量UV-B辐射对冬小麦幼苗形态及生理指标的影响[J].生态学杂志,29(2):314-318
刘清华,钟章成.2007.紫外线-B对银杏光合生理指标的影响[J].西南师范大学学报,28(2):148-153
刘文,巩健,张承仁,等.2008.紫外和干旱胁迫对转基因水稻生理和抗氧化酶的影响[J].种子,30(9):14-17
Lois R.1994.Accumulation of UV-absorbing flavonoids induced by UV-B radiation in Arabidopsis thaliana L[J].Planta,194(4):498-503
龙云,刘芸,钟章成,等.2009.酸雨和UV-B对玉米幼苗光合速率和抗氧化酶活性的影响[J].生态学报,29(9):4956-4965
Nakano Y A.1981.Hydrogen peroxide is scavenged by ascorbate-specific in spinach chloroplasts[J].Plant cell Physiol,22:867
Qu Y,Zhou Q,Yu B.2009.Effects of Zn2+and niflumic acid on photosynthesis in Glycine soja and Glycine max seedlings under Nacl stress[J].Environmental and Experimental Botany,65(2):304-309
彭祺,周青.2009.植物次生代谢响应UV-B辐射胁迫的生态学意义[J].中国生态农业学报,17(3):610-615
蒲晓宏,岳修乐,安黎哲.2017.植物对UV-B辐射的响应与调控机制[J].中国科学,47(8):818-828
祁虹,段留生,王树林,等.2017.全生育期UV-B辐射增强对棉花生长及光合作用的影响[J].中国生态农业学报,25(5):708-719
任健,李春阳.2005.种子植物对中波紫外辐射胁迫的响应研究进展[J].生态学杂志,24(3):315-320
Robberecht R,Caldwell M M,Billings W D.1980.Leaf ultraviolet optical properties along a latitudinal gradient in the arctic-alpine life zone[J].Ecology,61:612-619
Sancar A,Lindsey-Boltz L A,Unsal-Kacmaz K,et al.2004.Molecular mechanisms of mammalian DNA repair and the DNA damage checkpoints[J].Annual Review of Biochemistry,73:39-85
Tevini M,Teramura A H.1989.UV-B Effects of terrestrial plants[J].Photochem Photobiology,40:479-487
王海霞,刘文哲.2011.UV-B辐射增强对喜树叶片色素含量和形态结构的影响[J].中国农学通报,27(5):209-213
王子奇,查天山,贾昕,等.2017.油蒿光合参数季节动态及其与叶氮含量和比叶面积的关系[J].生态学杂志,36(4):916-924
吴兵,袁明璐,李胜,等.2008.高寒草甸植物抗氧化系统对长期增强UV-B辐射的响应[J].草业科学,25(5):69-73
岳明,王勋陵.2003.紫外线B辐射对几种植物种间竞争的影响[J].应用生态学报,14(8):1322-1326
张红霞,吴能表,胡丽涛,等.2010.不同强度UV-B辐射胁迫对蚕豆幼苗生长及叶绿素荧光特性的影响[J].西南师范大学学报,35(1):105-110
周党卫,韩发,滕中华,等.2002.UV-B辐射增强对植物光合作用的影响及植物的相关适应性研究[J].西北植物学报,(4):280-286
Dai Q,Yan B,Huang S,et al.1997.Response of oxidative stress defense systems in rice(Oryza sativa)leaves with supplemental UV-B radiation[J].Physiology Plantarum,101(2):301-308
杜照奎,李钧敏,钟章成.2014.花生幼苗对UV-B辐射增强的生理生化响应[J].西南大学学报,36(9):50-56
Ellman G L.1959.Tissue sulthydryl groups[J].Arch Biochem Biophys,82:70-77
Galatro A,Simontacchi M,Puntarulo S.2001.Free radical generation and antioxidant content in chloroplasts from soybean leaves exposed to ultraviolet-B[J].Physiology Plantarum,113(4):564-570
高俊凤.2006.植物生理学实验指导[M].北京:高等教育出版社.74-75;88-89
Giannopolitis C N,Ries S K,Superoxide Dismutases I.1977.Occurrence in higher plants[J].Plant Physiology,59(2):309-314
韩雯,韩榕.2015.不同时间的UV-B辐射对拟南芥幼苗生长的影响[J].植物学报,50(1):40-46
何永美,湛方栋,祖艳群,等.2013.大田条件下UV-B辐射对元阳梯田2 个地方水稻品种硅、类黄酮和总酚含量的影响[J].农业环境科学学报,(8):1500-1506
祭美菊,冯虎元,安黎哲,等.2002.增强的UVB辐射对植物影响的研究[J].应用生态学报,13(3):359-364
Jordan B R.1996.The effects of ultraviolet-B radiation on plants:a molecular perspective[J].Adv Bot Res,22:97-162
Jovanovic Z S,Milosevic J D,Radovic S R.2006.Antioxidative enzymes in the response of buckwheat(Fagopyrum esculentum Moench)to ultraviolet B radiation[J].Journal of Agricultural and Food Chemistry,54(25):9472-9478
Kerri L B.1994.Evidence for large upward trends of ultraviolet-B radiation lined to ozone depletion[J].Science,262:1032-1034
Knorzer O C,Durner J,Boger P.1996.Alteration in the antioxidative system of suspension cultivated soybean cells(Clycine max)induced by oxidative stress[J].Physiologic Plantarum,97(2):388-396
Law M,Charles S A,Halliwell B.1983.Glutathione and ascorbic acid in spinach(Spinacia olenacea)chloroplasts[J].Biochemical Journal,210:899-903
李惠梅,师生波.2010.长期增强UV-B辐射对高寒矮嵩草草甸植物光合作用的影响[J].西北植物学报,30(6):1186-1196
李良博,张连根,唐天向,等.2016.UV-B辐射增强对紫茎泽兰和艾草抗性生理特性的影响[J].西北植物学报,36(2):343-352
李元,何永美,祖艳群.2006.大田增强UV-B辐射对元阳梯田地方水稻茎秆性状和倒伏指数的影响[J].应用生态学报,17(1):123-126
李元,王勋陵.1998.紫外辐射增加对春小麦生理、产量和品质的影响[J].环境科学学报,18(5):504-509
刘丽丽,张文会,范颖伦,等.2010.不同剂量UV-B辐射对冬小麦幼苗形态及生理指标的影响[J].生态学杂志,29(2):314-318
刘清华,钟章成.2007.紫外线-B对银杏光合生理指标的影响[J].西南师范大学学报,28(2):148-153
刘文,巩健,张承仁,等.2008.紫外和干旱胁迫对转基因水稻生理和抗氧化酶的影响[J].种子,30(9):14-17
Lois R.1994.Accumulation of UV-absorbing flavonoids induced by UV-B radiation in Arabidopsis thaliana L[J].Planta,194(4):498-503
龙云,刘芸,钟章成,等.2009.酸雨和UV-B对玉米幼苗光合速率和抗氧化酶活性的影响[J].生态学报,29(9):4956-4965
Nakano Y A.1981.Hydrogen peroxide is scavenged by ascorbate-specific in spinach chloroplasts[J].Plant cell Physiol,22:867
Qu Y,Zhou Q,Yu B.2009.Effects of Zn2+and niflumic acid on photosynthesis in Glycine soja and Glycine max seedlings under Nacl stress[J].Environmental and Experimental Botany,65(2):304-309
彭祺,周青.2009.植物次生代谢响应UV-B辐射胁迫的生态学意义[J].中国生态农业学报,17(3):610-615
蒲晓宏,岳修乐,安黎哲.2017.植物对UV-B辐射的响应与调控机制[J].中国科学,47(8):818-828
祁虹,段留生,王树林,等.2017.全生育期UV-B辐射增强对棉花生长及光合作用的影响[J].中国生态农业学报,25(5):708-719
任健,李春阳.2005.种子植物对中波紫外辐射胁迫的响应研究进展[J].生态学杂志,24(3):315-320
Robberecht R,Caldwell M M,Billings W D.1980.Leaf ultraviolet optical properties along a latitudinal gradient in the arctic-alpine life zone[J].Ecology,61:612-619
Sancar A,Lindsey-Boltz L A,Unsal-Kacmaz K,et al.2004.Molecular mechanisms of mammalian DNA repair and the DNA damage checkpoints[J].Annual Review of Biochemistry,73:39-85
Tevini M,Teramura A H.1989.UV-B Effects of terrestrial plants[J].Photochem Photobiology,40:479-487
王海霞,刘文哲.2011.UV-B辐射增强对喜树叶片色素含量和形态结构的影响[J].中国农学通报,27(5):209-213
王子奇,查天山,贾昕,等.2017.油蒿光合参数季节动态及其与叶氮含量和比叶面积的关系[J].生态学杂志,36(4):916-924
吴兵,袁明璐,李胜,等.2008.高寒草甸植物抗氧化系统对长期增强UV-B辐射的响应[J].草业科学,25(5):69-73
岳明,王勋陵.2003.紫外线B辐射对几种植物种间竞争的影响[J].应用生态学报,14(8):1322-1326
张红霞,吴能表,胡丽涛,等.2010.不同强度UV-B辐射胁迫对蚕豆幼苗生长及叶绿素荧光特性的影响[J].西南师范大学学报,35(1):105-110
周党卫,韩发,滕中华,等.2002.UV-B辐射增强对植物光合作用的影响及植物的相关适应性研究[J].西北植物学报,(4):280-286