UV-B辐射增强对芦苇叶绿素荧光参数的影响
|
摘要
叶绿素荧光是光合作用的探针,可反映光合机构内一系列重要的调节过程.本文在露天条件下研究了不同UV-B辐照强度(对照:168μW·cm~2,低强度:210μW·cm~2,高强度:252μW·cm~2)对芦苇色素含量及叶绿素荧光参数的影响,探讨了芦苇光合生理对UV-B辐射的响应机制.结果表明:与自然光照相比,UV-B辐射增强对芦苇叶片表观造成一定的损伤,且随辐射梯度增大,损伤加剧.与自然光照相比,低辐射初期可促进芦苇叶片叶绿素a、叶绿素b、总叶绿素及类胡萝卜素含量增大,但随辐射时间延长抑制作用显现;高辐射下,芦苇叶绿素a、叶绿素b、总叶绿素及类胡萝卜素含量均显著降低.与自然光照相比,低辐射前期,叶绿素荧光参数无显著变化,随辐照时间延长,Fo、NPQ增高,Fm、Fv/Fm与Fv/Fo显著降低,ΦPSII值、q P降低.高辐射可显著增大Fo、NPQ,显著降低Fm、Fv/Fm、Fv/Fo、ΦPSII,降低q P.试验结束时,处理组NPQ均低于对照,且随辐射梯度增大,下降幅度加剧.UV-B辐射对PSII的破坏作用随辐射强度增大而增大.
Chlorophyll fluorescence is a probe of photosynthesis,which can reflect a series of important regulatory processes in the photosynthetic mechanism. The effects of different intensities UV-B radiation( control: 168 μW·cm~(-2),low-intensity: 210 μW·cm~(-2),high-intensity: 252 μW·cm~(-2)) on pigments content and chlorophyll fluorescence of Phragmites australis had been studied in the open air,in order to discuss the response mechanism of photosynthetic physiology to UV-B radiation. The results showed that: 1) Compared with the control,enhanced UV-B radiation caused a certain damage to the leaf appearance,with the increase of radiation intensity,the apparent damage degree became more seriously. 2) Compared with natural light,low radiation intensity increased the content of chlorophyll a,chlorophyll b,total chlorophyll and carotenoid. However,the inhibitory effect of the radiation was increased with the prolonged radiation time. High radiation intensity reduced significantly the content chlorophyll a,chlorophyll b,total chlorophyll and carotenoid. 3) Compared with natural light,There were no significant changes in chlorophyll fluorescence parameters at the early stage of low radiation. As the irradiation continues,low radiation intensity increased Foand NPQ,but reduced the ΦPSII,q P and significantly reduced the Fm,Fv/Fmand Fv/Fo. High radiation intensity significantly increased Foand NPQ,however reduced q P and significantly reduced Fm,Fv/Fm,Fv/FoandΦPSII. At the end of the experiment,NPQ was lower than the control group,and with the increasing radiation intensity,the degree increased. The damage of UV-B radiation to PSII increased with the increased radiation intensity.
Chlorophyll fluorescence is a probe of photosynthesis,which can reflect a series of important regulatory processes in the photosynthetic mechanism. The effects of different intensities UV-B radiation( control: 168 μW·cm~(-2),low-intensity: 210 μW·cm~(-2),high-intensity: 252 μW·cm~(-2)) on pigments content and chlorophyll fluorescence of Phragmites australis had been studied in the open air,in order to discuss the response mechanism of photosynthetic physiology to UV-B radiation. The results showed that: 1) Compared with the control,enhanced UV-B radiation caused a certain damage to the leaf appearance,with the increase of radiation intensity,the apparent damage degree became more seriously. 2) Compared with natural light,low radiation intensity increased the content of chlorophyll a,chlorophyll b,total chlorophyll and carotenoid. However,the inhibitory effect of the radiation was increased with the prolonged radiation time. High radiation intensity reduced significantly the content chlorophyll a,chlorophyll b,total chlorophyll and carotenoid. 3) Compared with natural light,There were no significant changes in chlorophyll fluorescence parameters at the early stage of low radiation. As the irradiation continues,low radiation intensity increased Foand NPQ,but reduced the ΦPSII,q P and significantly reduced the Fm,Fv/Fmand Fv/Fo. High radiation intensity significantly increased Foand NPQ,however reduced q P and significantly reduced Fm,Fv/Fm,Fv/FoandΦPSII. At the end of the experiment,NPQ was lower than the control group,and with the increasing radiation intensity,the degree increased. The damage of UV-B radiation to PSII increased with the increased radiation intensity.
引文
Azizpour K,Shakiba M R,Sima K K,et al.2010.Physiological response of spring durum wheat genotypes to salinity[J].Journal of Plant Nutrition,33(6):859-873
曹昀,王国祥,张聃.2008.干旱对芦苇幼苗生长和叶绿素荧光的影响[J].干旱区地理,31(6):862-869
Demmig B,Bjorkman O.1987.Comparison of the effect of excessive light on chlorophyll fluorescence(77K)and photon yield of O2evolution in leaves of higher plants[J].Planta,171:171-184
耿东梅,单立山,李毅.2014.土壤水分胁迫对红砂幼苗叶绿素荧光和抗氧化酶活性的影响[J].植物学报,49(3):282-291
韩雯,韩榕.2015.不同时间的UV-B辐射对拟南芥幼苗生长的影响[J].植物学报,50(1):40-46
李良博,唐天向,海梅荣,等.2015.植物对UV-B辐射增强的响应及其分子机制[J].中国农学通报,31(13):159-163
李良博,张连根,唐天向等.2016.UV-B辐射增强对紫茎泽兰和艾草抗性生理特性的影响[J].西北植物学报,36(2):343-352
李元,王勋陵.1998.紫外辐射增加对春小麦生理、产量和品质的影响[J].环境科学学报,18(5):504-509
Liang C J,Zhang G S,Zhou Q.2011.Effect of cerium on photosynthetic pigments and photochemical reaction activity in soybean seedling under ultraviolet-B radiation stress[J].Biological Trace Element Research,142(3):796-806
刘敏,李荣贵,范海,等.2007.UV-B辐射对烟草光合色素和几种酶的影响[J].西北植物学报,27(2):291-296
刘晓,贺俊芳,岳明.2011.荧光动力学方法研究光系统II原初传能过程对低剂量UV-B辐射的响应[J].生物物理学报,27(10):839-848
马晓丽,冀瑞萍,李亚莉.2016.一氧化氮对增强UV-B辐射后白菜叶绿素荧光特性和Rubisco活化酶的影响[J].核农学报,30(7):1440-1445
Mirecki R M,Teramura A H.1984.Effects of ultraviolet-B irradiance on soybean.V.The dependence of plant sensitivity on the photosynthetic photon flux density during and after leaf expansion[J].Plant Physiology,74:475-480.
祁虹,段留生,王树林,等.2017.全生育期UV-B辐射增强对棉花生长及光合作用的影响[J].中国生态农业学报,25(5):708-719
Rintamki E,Salo R,Aro E M.1994.Rapid turnover of the D1reactioncenter protein of photosystemⅡas a protectionmechanism against photoinhibition in a moss,Ceratodon purpureus(Hedw)Brid[J].Planta,193:520-529
Sullivan J H,Teramura A H,Dillenburg L R.1994.Growth and photo synthetic responses of field-grown sweetgum(Liquidambar styraciflua Hamamelidaceae)seedlings to UV-B radiation[J].Am J Bot,81:826-832
Teramura A H,Ziska L H,Sztein A E.1991.Changes in growth and photosynthetic capacity of rice with increased UV-B radiation[J].Physiologia Plantarum,83:373-380
Tevini M.1994.UV-B effects on terrestrial plants and aquatic organisms[J].Prog Bot,55:174-190
Tracewell C A,Vrettos J S,Bautista J A.2001.Carotenoid photo-oxidation in photosystemⅡ[J].Archives of Bioche-mistry and Biophysics,385:61-69
王锦旗,郑有飞,薛艳.2015.紫外辐射对菹草成株生理特性的影响[J].生态学报,35(18):5975-5983
徐军田,高坤山.2007.阳光紫外辐射对绿藻石莼光化学效率的影响.海洋学报[J].29(1):127-132
杨景宏,陈拓,王勋陵.2000.增强紫外线B辐射对小麦叶素膜组分和膜流动性的影响[J].植物生态学报,24(1):102-105
姚晓蕊,潘存德,李冬梅,等.2009.11种人工栽培树木叶绿素荧光特征比较[J].干旱区研究,26(4):555-560
余小龙,余树全,伊力塔,等.2011.UV-B辐射胁迫对细叶青冈幼苗叶绿素荧光特性的影响幼苗叶绿素荧光特性的影响[J].东北农业大学学报,42(10):114-119
张阿宏,齐孟文,张晔晖.2008.调制叶绿素荧光动力学参数及其计量关系的意义和公理化讨论[J].核农学报,22(6):909-912
张雷明,上官周平,毛明策,等.2003.长期施氮对旱地小麦灌浆期叶绿素荧光参数的影响[J].应用生态学报,14(5):695-698
张美萍,王勇,韩榕,等.2014.增强UV-B辐射对水稻叶绿素荧光参数的影响[J].山西师范大学学报,28(1):66-70
张守仁.1999.叶绿素荧光动力学参数的意义及讨论[J].植物学通报,16(4):444-448
赵天宏,刘轶鸥,王岩,等.2013.浓度升高和UV-B辐射增强对大豆叶片叶绿素含量和活性氧代谢的影响[J].应用生态学报,24(5):1277-1283
周党卫,韩发,滕中华,等.2002.UV-B辐射增强对植物光合作用的影响及植物的相关适应性研究[J].西北植物学报,(4):280-286
訾先能,陈宗瑜,郭世昌,等.2006.UV-B辐射的增强对作物形态及生理功能的影响[J].中国农业气象,27(2):102-106
邹琦.1995.植物生理生化实验指导[M].北京:中国农业出版社.36-38
左园园,刘庆,林波,等.2005.短期增强UV-B辐射对青榨槭幼苗生理特性的影响[J].应用生态学报,16(9):1682-1686
曹昀,王国祥,张聃.2008.干旱对芦苇幼苗生长和叶绿素荧光的影响[J].干旱区地理,31(6):862-869
Demmig B,Bjorkman O.1987.Comparison of the effect of excessive light on chlorophyll fluorescence(77K)and photon yield of O2evolution in leaves of higher plants[J].Planta,171:171-184
耿东梅,单立山,李毅.2014.土壤水分胁迫对红砂幼苗叶绿素荧光和抗氧化酶活性的影响[J].植物学报,49(3):282-291
韩雯,韩榕.2015.不同时间的UV-B辐射对拟南芥幼苗生长的影响[J].植物学报,50(1):40-46
李良博,唐天向,海梅荣,等.2015.植物对UV-B辐射增强的响应及其分子机制[J].中国农学通报,31(13):159-163
李良博,张连根,唐天向等.2016.UV-B辐射增强对紫茎泽兰和艾草抗性生理特性的影响[J].西北植物学报,36(2):343-352
李元,王勋陵.1998.紫外辐射增加对春小麦生理、产量和品质的影响[J].环境科学学报,18(5):504-509
Liang C J,Zhang G S,Zhou Q.2011.Effect of cerium on photosynthetic pigments and photochemical reaction activity in soybean seedling under ultraviolet-B radiation stress[J].Biological Trace Element Research,142(3):796-806
刘敏,李荣贵,范海,等.2007.UV-B辐射对烟草光合色素和几种酶的影响[J].西北植物学报,27(2):291-296
刘晓,贺俊芳,岳明.2011.荧光动力学方法研究光系统II原初传能过程对低剂量UV-B辐射的响应[J].生物物理学报,27(10):839-848
马晓丽,冀瑞萍,李亚莉.2016.一氧化氮对增强UV-B辐射后白菜叶绿素荧光特性和Rubisco活化酶的影响[J].核农学报,30(7):1440-1445
Mirecki R M,Teramura A H.1984.Effects of ultraviolet-B irradiance on soybean.V.The dependence of plant sensitivity on the photosynthetic photon flux density during and after leaf expansion[J].Plant Physiology,74:475-480.
祁虹,段留生,王树林,等.2017.全生育期UV-B辐射增强对棉花生长及光合作用的影响[J].中国生态农业学报,25(5):708-719
Rintamki E,Salo R,Aro E M.1994.Rapid turnover of the D1reactioncenter protein of photosystemⅡas a protectionmechanism against photoinhibition in a moss,Ceratodon purpureus(Hedw)Brid[J].Planta,193:520-529
Sullivan J H,Teramura A H,Dillenburg L R.1994.Growth and photo synthetic responses of field-grown sweetgum(Liquidambar styraciflua Hamamelidaceae)seedlings to UV-B radiation[J].Am J Bot,81:826-832
Teramura A H,Ziska L H,Sztein A E.1991.Changes in growth and photosynthetic capacity of rice with increased UV-B radiation[J].Physiologia Plantarum,83:373-380
Tevini M.1994.UV-B effects on terrestrial plants and aquatic organisms[J].Prog Bot,55:174-190
Tracewell C A,Vrettos J S,Bautista J A.2001.Carotenoid photo-oxidation in photosystemⅡ[J].Archives of Bioche-mistry and Biophysics,385:61-69
王锦旗,郑有飞,薛艳.2015.紫外辐射对菹草成株生理特性的影响[J].生态学报,35(18):5975-5983
徐军田,高坤山.2007.阳光紫外辐射对绿藻石莼光化学效率的影响.海洋学报[J].29(1):127-132
杨景宏,陈拓,王勋陵.2000.增强紫外线B辐射对小麦叶素膜组分和膜流动性的影响[J].植物生态学报,24(1):102-105
姚晓蕊,潘存德,李冬梅,等.2009.11种人工栽培树木叶绿素荧光特征比较[J].干旱区研究,26(4):555-560
余小龙,余树全,伊力塔,等.2011.UV-B辐射胁迫对细叶青冈幼苗叶绿素荧光特性的影响幼苗叶绿素荧光特性的影响[J].东北农业大学学报,42(10):114-119
张阿宏,齐孟文,张晔晖.2008.调制叶绿素荧光动力学参数及其计量关系的意义和公理化讨论[J].核农学报,22(6):909-912
张雷明,上官周平,毛明策,等.2003.长期施氮对旱地小麦灌浆期叶绿素荧光参数的影响[J].应用生态学报,14(5):695-698
张美萍,王勇,韩榕,等.2014.增强UV-B辐射对水稻叶绿素荧光参数的影响[J].山西师范大学学报,28(1):66-70
张守仁.1999.叶绿素荧光动力学参数的意义及讨论[J].植物学通报,16(4):444-448
赵天宏,刘轶鸥,王岩,等.2013.浓度升高和UV-B辐射增强对大豆叶片叶绿素含量和活性氧代谢的影响[J].应用生态学报,24(5):1277-1283
周党卫,韩发,滕中华,等.2002.UV-B辐射增强对植物光合作用的影响及植物的相关适应性研究[J].西北植物学报,(4):280-286
訾先能,陈宗瑜,郭世昌,等.2006.UV-B辐射的增强对作物形态及生理功能的影响[J].中国农业气象,27(2):102-106
邹琦.1995.植物生理生化实验指导[M].北京:中国农业出版社.36-38
左园园,刘庆,林波,等.2005.短期增强UV-B辐射对青榨槭幼苗生理特性的影响[J].应用生态学报,16(9):1682-1686