四逆酸枣仁汤合方对失眠大鼠海马GABAARα1、γ2及NKCC1的影响
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摘要
目的:观察四逆酸枣仁汤合方对失眠大鼠海马GABAARα1、γ2及NKCC1的影响。方法:采用SD大鼠腹腔注射对氯苯丙氨酸(PCPA)复制失眠模型,将复制成功的模型大鼠随机分为模型组、西药组、四逆散组、酸枣仁汤组、合方组,并设置空白对照组,各组给予相应药物连续7 d,模型组和正常组生理盐水连续灌胃7 d,并观察各组大鼠的一般活动状态和体质量增量。采用实时荧光定量PCR检测海马中GABAARα1、γ2和NKCC1mRNA的表达;采用Western Blot的方法检测海马中GABAARα1、γ2和NKCC1蛋白的表达。结果:四逆酸枣仁汤合方可以提高海马中GABAARα1、γ2mRNA和蛋白的表达,降低海马中NKCC1mRNA和蛋白的表达。结论:四逆酸枣仁汤合方可以通过调节失眠大鼠海马中GABAARα1、γ2和NKCC1mRNA和蛋白的表达量来改善大鼠的睡眠节律。
Objective: To observe the effects of Sini-Suanzaoren Formula( combined formula of Sini San and Suanzaoren Decoction) on GABAARα1,GABAARγ2 and NKCC1 of hippocampus in rats with insomnia. Methods: Insomnia model was established by PCPA injection in SD rats. Successful models were randomly divided into the model group,the western medicine group,Sini San group,Suanzaoren Decoction group,and Sini-Suanzaoren Formula group. The normal rats were taken as the blank control. After seven days of intervention,the general activity and body mass of the rats were observed,mRNA expressions of GABAARα1,GABAARγ2 and NKCC1 were detected by PCR assay. Protein expressions of GABAARα1,GABAARγ2 and NKCC1 were detected by Western Blot method. Results: Sini-Suanzaoren Formula could increase mRNA and protein expressions of GABAARα1 and GABAARγ2,reduce mRNA and protein expressions of NKCC1. Conclusion: Sini-Suanzaoren Formula can improve sleep rhythm by regulating mRNA and protein expressions of GABAARα1,GABAARγ2 and NKCC1 of hippocampus in rats with insomnia.
Objective: To observe the effects of Sini-Suanzaoren Formula( combined formula of Sini San and Suanzaoren Decoction) on GABAARα1,GABAARγ2 and NKCC1 of hippocampus in rats with insomnia. Methods: Insomnia model was established by PCPA injection in SD rats. Successful models were randomly divided into the model group,the western medicine group,Sini San group,Suanzaoren Decoction group,and Sini-Suanzaoren Formula group. The normal rats were taken as the blank control. After seven days of intervention,the general activity and body mass of the rats were observed,mRNA expressions of GABAARα1,GABAARγ2 and NKCC1 were detected by PCR assay. Protein expressions of GABAARα1,GABAARγ2 and NKCC1 were detected by Western Blot method. Results: Sini-Suanzaoren Formula could increase mRNA and protein expressions of GABAARα1 and GABAARγ2,reduce mRNA and protein expressions of NKCC1. Conclusion: Sini-Suanzaoren Formula can improve sleep rhythm by regulating mRNA and protein expressions of GABAARα1,GABAARγ2 and NKCC1 of hippocampus in rats with insomnia.
引文
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[11]Kristopher T.The role and the mechanism ofγ-aminobutyric acid during central nervous system development[J].Neurosci Bull,2008,24(3):195-200.
[12]赵小明,张健.不同强度电针治疗对氯苯丙氨酸失眠大鼠模型的相关机制[J].医疗卫生装备,2016,37(5):13-16.
[13]高家荣,季文博,姜辉,等.酸枣仁-五味子药对醇水双提物对PCPA致失眠大鼠氨基酸类神经递质的影响[J].中药材,2013,36(10):1635-1639.
[2]贾玉,贾跃进,郑晓琳.中医对失眠认识的探讨及展望[J].中华中医药杂志,2015,30(1):163-166.
[3]马捷,李峰,宋月晗,等.从肝论治失眠的文献研究[J].中华中医药杂志,2012,27(4):1076-1080.
[4]蒋洁,赵百孝,哈略,等.不同波形电针对PCPA致失眠大鼠下丘脑5-HT和Glu、GABA含量的影响[J].上海针灸杂志,2015,34(7):678-681.
[5]Adrien J,Laguzzi R,Bourgoin S,et al,Sleep in the rate raphe lesion:pharmacologic of the systeme serotoninergique[J].Waking and Sleeping,1980(4):80-85.
[6]肖成荣,马增春,李海静,等.PCPA失眠大鼠模型的制作及其机制[J].毒理学杂志,2007,21(4):326.
[7]Riemann D,Spiegelhalder K,Feige B,et al.The hyperarousal model of insomnia:a review of the concept and its evidence[J].Sleep Med Rev,2010,14:19-31.
[8]Levenson JC,Kay DB,Buysse DJ.The pathophysiology of insomnia[J].Chest,2015(147):1179-1792.
[9]Laura Palagini,Knut Biber,Dieter Riemann.The genetics of insomniae Evidence for epigenetic mechanisms?[J].Sleep Medicine Reviews,2014,18:225-235.
[10]刘祖丽,唐成林,余敏,等.不同强度电针对PCPA失眠大鼠下丘脑γ-氨基丁酸及受体的影响[J].生命科学研究,2011,15(3):236-240.
[11]Kristopher T.The role and the mechanism ofγ-aminobutyric acid during central nervous system development[J].Neurosci Bull,2008,24(3):195-200.
[12]赵小明,张健.不同强度电针治疗对氯苯丙氨酸失眠大鼠模型的相关机制[J].医疗卫生装备,2016,37(5):13-16.
[13]高家荣,季文博,姜辉,等.酸枣仁-五味子药对醇水双提物对PCPA致失眠大鼠氨基酸类神经递质的影响[J].中药材,2013,36(10):1635-1639.