NOK的表达变化与结直肠癌患者临床病理特征及术后预后的关系
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摘要
目的分析癌组织中NOK表达情况与结直肠癌患者临床病理及手术预后的关系。方法回顾性分析2011年1月~2015年1月于我院接受手术治疗的80例结直肠癌患者的临床资料。所有患者术中均留取癌组织标本,采用免疫组化法测定癌组织NOK、表皮生长因子受体(EGFR)的表达情况。收集所有患者的临床病理及预后资料,分析癌组织中NOK表达与临床病理特征、EGFR表达及预后的关系。结果 80例结直肠癌患者中NOK表达阳性59例,阳性率为73.75%,EGFR表达阳性61例,阳性率为76.25%;临床分期为Ⅲ~Ⅳ期、浸润深度为T3~T4、合并淋巴结转移、合并远处转移的结直肠癌患者NOK阳性率和EGFR阳性率显著高于临床分期为Ⅰ~Ⅱ期、浸润深度为T1~T2、无淋巴结转移、无远处转移的患者(P<0.05)。肿瘤复发、转移及死亡患者的NOK、EGFR表达阳性率明显高于肿瘤未复发、转移及存活患者(P<0.05)。临床分期、肿瘤浸润深度、淋巴结转移、远处转移、NOK阳性、EGFR阳性均为影响结直肠癌患者手术预后的独立危险因素。结论结直肠癌患者癌组织中NOK蛋白表达阳性率高,与EGFR表达一致性较高,且NOK蛋白表达水平与患者临床分期、浸润深度、淋巴结转移、远处转移及手术预后存在明显相关性。
Objective To analyze the relationship between the expression of NOK in cancer tissues and the clinical pathology and prognosis of patients with colorectal cancer.Methods The clinical data of 80 patients with colorectal cancer who underwent surgical treatment in our hospital from January 2011 to January 2015 were retrospectively analyzed.The cancer tissue samples of all patients were collected to determine the expression of NOK and epidermal growth factor receptor(EGFR) by immunohistochemical method,and the clinical pathology and prognosis of all patients were collected to analyze the relationship between the expression of NOK and the clinical pathology,the expression of EGFR and the prognosis.Results Among 80 patients,the expression of NOK was positive in 59 cases and the positive rate was 73.75%,while the expression of EGFR was positive in 61 cases and the positive rate was 76.25%.The positive rates of NOK and EGFR in patients at clinical stage Ⅲ~Ⅳ,patients with infiltration depth of T3~T4,patients with lymph node metastasis and patients with distant metastasis were higher than those in patients at clinical stageⅠ~Ⅱ,patients with infiltration depth of T1~T2,patients without lymph node metastasis or patients without distant metastasis(P<0.05).The positive rates of NOK and EGFR in patients with tumor recurrence,metastasis and dead patients were higher than those in patients without recurrence,metastasis and survival patients(P<0.05).Clinical stage,depth of tumor invasion,lymph node metastasis,distant metastasis,positive NOK,and positive EGFR were independent on risk factors affecting the prognosis of patients with colorectal cancer.Conclusion The positive expression rate of NOK protein in cancer tissues of patients with colorectal cancer is high,and the consistency with expression of EGFR is relatively higher,and the expression level of NOK protein is significantly correlated with the clinical stage,depth of invasion,lymph node metastasis,distant metastasis and prognosis.
Objective To analyze the relationship between the expression of NOK in cancer tissues and the clinical pathology and prognosis of patients with colorectal cancer.Methods The clinical data of 80 patients with colorectal cancer who underwent surgical treatment in our hospital from January 2011 to January 2015 were retrospectively analyzed.The cancer tissue samples of all patients were collected to determine the expression of NOK and epidermal growth factor receptor(EGFR) by immunohistochemical method,and the clinical pathology and prognosis of all patients were collected to analyze the relationship between the expression of NOK and the clinical pathology,the expression of EGFR and the prognosis.Results Among 80 patients,the expression of NOK was positive in 59 cases and the positive rate was 73.75%,while the expression of EGFR was positive in 61 cases and the positive rate was 76.25%.The positive rates of NOK and EGFR in patients at clinical stage Ⅲ~Ⅳ,patients with infiltration depth of T3~T4,patients with lymph node metastasis and patients with distant metastasis were higher than those in patients at clinical stageⅠ~Ⅱ,patients with infiltration depth of T1~T2,patients without lymph node metastasis or patients without distant metastasis(P<0.05).The positive rates of NOK and EGFR in patients with tumor recurrence,metastasis and dead patients were higher than those in patients without recurrence,metastasis and survival patients(P<0.05).Clinical stage,depth of tumor invasion,lymph node metastasis,distant metastasis,positive NOK,and positive EGFR were independent on risk factors affecting the prognosis of patients with colorectal cancer.Conclusion The positive expression rate of NOK protein in cancer tissues of patients with colorectal cancer is high,and the consistency with expression of EGFR is relatively higher,and the expression level of NOK protein is significantly correlated with the clinical stage,depth of invasion,lymph node metastasis,distant metastasis and prognosis.
引文
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[3]Konishi H,Shirabe K,Nakagawara H,et al.Suppression of silent information regulator 1 activity in noncancerous tissues of hepatocellular carcinoma:Possible association with non-B non-C hepatitis pathogenesis[J].Cancer Sci,2015,106(5):542-549.doi:10.1111/cas.12653.
[4]Li Y.Colorectal cancer:An intravenous probe for colorectal cancer screening[J].Nat Rev Gastroenterol Hepatol,2015,12(10):554-556.doi:10.1038/nrgastro.2015.157.
[5]Li Y,Lyu Z,Zhao L,et al.Prognostic value of MGMT methylation in colorectal cancer:a meta-analysis and literature review[J].Tumour Biol,2015,36(3):1595-1601.doi:10.1007/s13277-014-2752-9.
[6]Coffey JC,Dockery P.Colorectal cancer:Surgery for colorectal cancer-standardization required[J].Nat Rev Gastroenterol Hepatol,2016,13(5):256-257.doi:10.1038/nrgastro.2016.40.
[7]Cao Y,Meyerhardt JA,Chan AT,et al.Television watching and colorectal cancer survival in men[J].Cancer Causes Control,2015,26(10):1467-1476.doi:10.1007/s10552-015-0645-x.
[8]Nazari M,Muddiman DC.Polarity switching mass spectrometry imaging of healthy and cancerous hen ovarian tissue sections by infrared matrix-assisted laser desorption electrospray ionization(IR-MALDESI)[J].Analyst,2016,141(2):595-605.doi:10.1039/c5an01513h.
[9]Pedersen KE,Elwood M.Current international developments in population screening for colorectal cancer[J].ANZ J Surg,2002,72(7):507-512.
[10]Heine-Br?ring RC,Winkels RM,Renkema JM,et al.Dietary supplement use and colorectal cancer risk:a systematic review and meta-analyses of prospective cohort studies[J].Int J Cancer,2015,136(10):2388-2401.doi:10.1002/ijc.29277.
[11]Van der Geest LG,Lam-Boer J,Koopman M,et al.Nationwide trends in incidence,treatment and survival of colorectal cancer patients with synchronous metastases[J].Clin Exp Metastasis,2015,32(5):457-465.doi:10.1007/s10585-015-9719-0.
[12]Kohzadi S,Sheikhesmaili F,Rahehagh R,et al.Evaluation of trace element concentration in cancerous and non-cancerous tissues of human stomach[J].Chemosphere,2017,184:747-752.doi:10.1016/j.chemosphere.2017.06.071.
[13]Tan SC,Ismail MP,Duski DR,et al.Identification of Optimal Reference Genes for Normalization of RT-q PCR Data in Cancerous and Non-Cancerous Tissues of Human Uterine Cervix[J].Cancer Invest,2017,35(3):163-173.doi:10.1080/07357907.2017.1278767.
[14]Sircan-Ku?uksayan A,Denkceken T,Canpolat M.Differentiating cancerous tissues from noncancerous tissues using single-fiber reflectance spectroscopy with different fiber diameters[J].J Biomed Opt,2015,20(11):115007.doi:10.1117/1.JBO.20.11.115007.
[15]Brozek-Pluska B,Kopec M,Niedzwiecka I,et al.Labelfree determination of lipid composition and secondary protein structure of human salivary noncancerous and cancerous tissues by Raman microspectroscopy[J].Analyst,2015,140(7):2107-2113.doi:10.1039/c4an01394h.