非小细胞肺癌患者胸膜转移瘤中NOK的表达及其与生存期的相关性
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摘要
目的:探讨非小细胞肺癌(NSCLC)患者胸膜转移瘤中NOK表达及其与患者生存期的相关性。方法:选取我科2014年至2015年收治的Ⅳ期NSCLC患者67例,用软式胸腔镜取胸膜转移瘤和瘤旁胸膜,采用免疫组化法测定NOK的表达情况,分析胸膜转移瘤和瘤旁胸膜中NOK的表达的无差异性;对患者进行随访,并分析胸膜转移瘤和瘤旁胸膜中NOK的表达与患者生存期的相关性。结果:1Ⅳ期NSCLC患者胸膜转移瘤中NOK的表达明显高于瘤旁胸膜,具有统计学差异(P<0.05);2Ⅳ期NSCLC患者胸膜转移瘤中NOK的表达量与患者的生存期呈负相关,瘤旁胸膜中NOK的表达量与患者的生存期无显著相关性。结论:NSCLC患者胸膜转移瘤中NOK的表达异常上调,且与患者的生存期呈负相关,可做为判断Ⅳ期NSCLC患者生存期的参考指标。
Objective: To investigate the NOK expression in NSCLC patients and its correlation with the prognosis. Methods: 67 cases of NSCLC patients at stage Ⅳ with pleural metastases who admitted in our division from 2014 to 2015 were selected, and metastatic tumor of pleura and pleura beside the tumor were obtained using soft thoracoscopic, then the expression of NOK in the two kinds of issues were detected by immunohistochemistry, then the correlation of NOK expression in metastatic tumor of pleura and pleura beside the tumor with the prognosis of patients were analyzed. Results:(1) The expression of NOK in metastatic tumor of pleura was apparently higher than that from pleura beside the tumor(P<0.05);(2)The expression of NOK in metastatic tumor of pleura was inversely related to the survival period of patients(P<0.05), while there was no significant relationship between the expression of NOK in pleura beside the tumor and survival of patients. Conclusions: The expression of NOK in metastatic tumor of pleura was inversely related to the survival period of NSCLC patients at stage Ⅳ, which could be used as the standard for assessing the survival of NSCLC patients at stage Ⅳ.
Objective: To investigate the NOK expression in NSCLC patients and its correlation with the prognosis. Methods: 67 cases of NSCLC patients at stage Ⅳ with pleural metastases who admitted in our division from 2014 to 2015 were selected, and metastatic tumor of pleura and pleura beside the tumor were obtained using soft thoracoscopic, then the expression of NOK in the two kinds of issues were detected by immunohistochemistry, then the correlation of NOK expression in metastatic tumor of pleura and pleura beside the tumor with the prognosis of patients were analyzed. Results:(1) The expression of NOK in metastatic tumor of pleura was apparently higher than that from pleura beside the tumor(P<0.05);(2)The expression of NOK in metastatic tumor of pleura was inversely related to the survival period of patients(P<0.05), while there was no significant relationship between the expression of NOK in pleura beside the tumor and survival of patients. Conclusions: The expression of NOK in metastatic tumor of pleura was inversely related to the survival period of NSCLC patients at stage Ⅳ, which could be used as the standard for assessing the survival of NSCLC patients at stage Ⅳ.
引文
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[2]Chen P,Li WM,Lu Q,et al.Clinicopathologic features and prognostic implications of NOK/STYK1 protein expression in non-small cell lung cancer[J].BMC Cancer,2014,4(14):402
[3]Orang AV,Safaralizadeh R,Hosseinpour Feizi MA,et al.Diagnostic relevance of overexpressed serine threonine tyrosine kinase/novel oncogene with kinase domain(STYK1/NOK)m RNA in colorectal cancer[J].Asian Pac J Cancer Prev,2014,15(16):6685-6689
[4]Li J,Wu F,Sheng F,et al.NOK/STYK1 interacts with GSK-3βand mediates Ser9 phosphorylation through activated Akt[J].FEBS Lett,2012,586(21):3787-3792
[5]Ding X,Jiang QB,Li R,et al.NOK/STYK1 has a strong tendency towards forming aggregates and colocalises with epidermal growth factor receptor n endosomes[J].Biochem Biophys Res Commun,2012,421(3):468-473
[6]Klumphu P,Lipshutz BH."Nok":a phytosterol-based amphiphile enabling transition-metal-catalyzed couplings in water at room temperature[J].J Org Chem,2014,79(3):888-900
[7]KivelT.Increasing collaboration in NOK 2012,the XXXX Nordic Congress of Ophthalmology[J].Acta Ophthalmol,2012,90(5):397-398
[8]Zhang Z,Jiang T,Deng Y,et al.Expression and its Clinical Significance of NOK,EGFR in NSCLC[J].Chinese Journal of Lung Cancer,2009,12(9):983-988
[9]Greenburg G,Hay ED.Epithelia suspended in collagen gels can lose polarity and express characteristics of migrating mesenchymal cells[J].The Journal of cell biology,1982,95(1):333-339
[10]Scanlon CS,Van Tubergen EA,Inglehart RC,et al.Biomarkers of epithelial-mesenchymal transition in squamous cell carcinoma[J].Journal of dental research,2013,92(2):114-121
[11]刘海涛.NOK与FGFR2在NSCLC中的表达及与NSCLC增殖的相关性研究[D].西安:第四军医大学,2014:1-62Liu Hai-tao.Research on the reationship between expressions of NOK and FGFR2 and proliferation of non-small cell lung cancer[D].Xi'an:The Fourth Military Medical University,2014:1-62
[12]Turley EA,Veiseh M,Radisky DC,et al.Mechanisms of disease:epithelial-mesenchymal transition--does cellular plasticity fuel neoplastic progression?[J].Nature clinical practice Oncology,2008,5(5):280-290
[13]Jung M Y,Chong A,Seon H J,et al.Indeterminate pleural metastasis on contrast-enhanced chest CT in non-small cell lung cancer:improved differential diagnosis with F-FDG PET/CT[J].Annals of Nuclear Medicine,2012,26(4):327-336
[14]Xu Q,Wang Y,Liu H,et al.Treatment outcome for patients with primary NSCLC and synchronous solitary metastasis[J].Clinical&Translational Oncology,2013,15(10):1-8
[15]Feng Q L,Shi H R,Song H H.Expressions of Novel Oncogene with Kinase-Domain and Phospho-Signal Transducer and Activator of Transcription 3 in Ovarian Cancer and the Significances[J].Chinese General Practice,2010,28(13):1301-1303
[16]Amachika T,Kobayashi D R,Tsuji N,et al.Diagnostic relevance of overexpressed m RNA of novel oncogene with kinase-domain(NOK)in lung cancers[J].Lung Cancer,2007,56(3):337-340
[17]Li L,Xin Zi Y,Tie Shi L,et al.A novel protein tyrosine kinase NOK that shares homology with platelet-derived growth factor/fibroblast growth factor receptors induces tumorigenesis and metastasis in nude mice[J].Cancer Research,2004,64(10):3491-3499
[18]Ying-Hua L,Yin-Yin W,Shan Z,et al.Transmembrane Helix of Novel Oncogene with Kinase-Domain(NOK)Influences Its Oligomerization and Limits the Activation of RAS/MAPK Signaling[J].Molecules&Cells,2009,27(1):39-45
[19]Takashi K,Daisuke K,Naoki T,et al.Overexpression of serine threonine tyrosine kinase 1/novel oncogene with kinase domain m RNA in patients with acute leukemia.[J].Experimental Hematology,2009,37(7):824-830
[20]Matsumoto S,Tanaka F,Sato K,et al.Monitoring with a noninvasive bioluminescent in vivo imaging system of pleural metastasis of lung carcinoma[J].Lung Cancer,2009,66(1):75-79