彝族“黄药”艾纳香中黄酮的提取工艺优选研究
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摘要
目的:优选艾纳香中总黄酮的提取工艺。方法:以乙醇浓度、提取时间、料液比、提取次数为因素,以总黄酮提取率为指标,采用正交试验优选提取工艺。结果:最佳条件为用16倍量的50%乙醇,提取3次,每次1. 5 h。结论:优选的工艺稳定、合理,可用于艾纳香中总黄酮的提取。
Objective To optimize the extraction process of total flavonoids from Huang Yao( Blumea balsamifera DC.) of Yi Nationality. Methods With the concentration of ethanol,extraction time,ratio of material to liquid and extraction times as the factors,the extraction rate of total flavonoids was used as the index,and the extraction process was optimized by orthogonal test. Results The optimal conditions were as follows: 16 times the amount of 50% ethanol,extracted 3 times,each time 1. 5 h. Conclusion The preferred process is stable and reasonable and can be used for the extraction of total flavonoids from Ayurveda.
Objective To optimize the extraction process of total flavonoids from Huang Yao( Blumea balsamifera DC.) of Yi Nationality. Methods With the concentration of ethanol,extraction time,ratio of material to liquid and extraction times as the factors,the extraction rate of total flavonoids was used as the index,and the extraction process was optimized by orthogonal test. Results The optimal conditions were as follows: 16 times the amount of 50% ethanol,extracted 3 times,each time 1. 5 h. Conclusion The preferred process is stable and reasonable and can be used for the extraction of total flavonoids from Ayurveda.
引文
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[14]黄永林,赵志国,文永新.不同部位艾纳香中总黄酮的含量测定[J].广西植物,2006,26(4):453-455.
[15]罗夫来,张云淋,赵致.正交设计优化艾纳香总黄酮提取工艺研究[J].中华中医药学刊,2013,31(12):2735-2737.
[16]和银霞,董一昕,冶泽青.艾纳香总黄酮提取工艺优化及其含量测定方法研究[J].辽宁中医药大学学报,2016,18(11)35-37.
[17]黄火强,丁福荣,李璞,等.彝族“黄药”艾纳香化学成分[J].中国实验方剂学杂志,2014,20(21):106-108.
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[2]庞玉新,谢小丽,陈振夏,等.艾纳香本草考证[J].贵州农业科学,2014,42(6):10-13.
[3]庞玉新.艾纳香遗传多样性与艾纳香属分子系统学研究[D].北京:北京中医药大学,2010.
[4]杨本雷.中国彝族药学[M].昆明:云南民族出版社,2004:156-157.
[5]黄火强,蒋慧嫦,张亚琛,等.彝族“黄药”(艾纳香)二氯甲烷提取物对Lewis肺癌小鼠抑瘤作用的研究[J].云南农业大学学报(自然科学),2016,31(6):1058-1064.
[6]林永成,龙康侯,邓一军.中药艾纳香化学成分的研究[J].中山大学学报(自然科学版),1988(2):77-80.
[7]N Saewan.Anti-tyrosinase and anti-cancer activities of flavonoids from Blumea balsamifera DC[J],2011,5(6):1018-1025.
[8]陈铭.艾纳香的活性成分研究[D].上海:上海交通大学,2009.
[9]谢月英,谢朋飞,黄宝优,等.艾纳香属植物化学成分及药理活性研究概况[J].中国现代中药,2016,18(8):1071-1076.
[10]严启新,谭道鹏,康晖,等.艾纳香中的黄酮类化学成分[J].中国实验方剂学杂志,2012,18(5):86-89.
[11]Norikura Toshio,Kojima-Yuasa Akiko,Shimizu Miki,et al.Mechanism of growth inhibitory effect of Blumea balsamifera extract in hepatocellular carcinoma[J],2008,72(5):1183-1189.
[12]Hiroo H,Yasuaki Y,Kanki K,et al.Dihydroflavonol BB-1,an extract of natural plant Blumea balsamifera,abrogates TRAIL resistance in leukemia cells[J].Blood,2006(107):679-688.
[13]LF Shyur,JH Tsung,JH Chen,et al.Antioxidant properties of extracts from medicinal plants popularly used in Taiwan[J].Int J Appl Sci Engin,2005,3(3):195-202.
[14]黄永林,赵志国,文永新.不同部位艾纳香中总黄酮的含量测定[J].广西植物,2006,26(4):453-455.
[15]罗夫来,张云淋,赵致.正交设计优化艾纳香总黄酮提取工艺研究[J].中华中医药学刊,2013,31(12):2735-2737.
[16]和银霞,董一昕,冶泽青.艾纳香总黄酮提取工艺优化及其含量测定方法研究[J].辽宁中医药大学学报,2016,18(11)35-37.
[17]黄火强,丁福荣,李璞,等.彝族“黄药”艾纳香化学成分[J].中国实验方剂学杂志,2014,20(21):106-108.
[18]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:89.