黔产艾纳香黄酮抗氧自由基的研究
|
摘要
[目的]研究黔产艾纳香黄酮对氧自由基的清除能力。[方法]以黔产艾纳香为研究对象,提取其黄酮;采用邻二氨菲-Fe(Ⅱ)/H2O2体系产生羟自由基(·OH),用联苯三酚法在碱性溶液中自氧化反应产生超氧阴离子自由基(O2-·),并采用分光光度法测定黔产艾纳香黄酮对O2-·和·OH的清除作用。[结果]高剂量组的黔产艾纳香黄酮对O2-·具有明显的清除及抑制作用,对·OH也有一定的清除作用,清除率为(19.82%±0.17%)。[结论]黔产艾纳香黄酮对O2-·具有明显清除及抑制作用,对·OH也有一定的清除作用。
[Objective] To study the scavenging action of Blumea flavanones on oxygen free radical.[Method] Auto-oxidation in alkalcsent solution with the help of pyrogallol can produce O2-· and the system of 1,10-phenathroline-Fe(Ⅱ) /H2O2 can generate ·OH.Spectrophotometry was used to observe the effects of Blumea flavanones upon O2-· and ·OH in vitro.[Result]High dosage Blumea flavanones has obvious scavenging and inhibiting effect on O2-·,and can reduce the level of ·OH by(19.82%-0.17%).[Conclusion] Guizhou's Blumea flavanones could scavenge and inhibit O2-· significantly and could scavenge ·OH as well.
[Objective] To study the scavenging action of Blumea flavanones on oxygen free radical.[Method] Auto-oxidation in alkalcsent solution with the help of pyrogallol can produce O2-· and the system of 1,10-phenathroline-Fe(Ⅱ) /H2O2 can generate ·OH.Spectrophotometry was used to observe the effects of Blumea flavanones upon O2-· and ·OH in vitro.[Result]High dosage Blumea flavanones has obvious scavenging and inhibiting effect on O2-·,and can reduce the level of ·OH by(19.82%-0.17%).[Conclusion] Guizhou's Blumea flavanones could scavenge and inhibit O2-· significantly and could scavenge ·OH as well.
引文
[1]贵州省中药研究所.贵州中药资源[M].北京:中国医药科技出版社,1992:262.
[2]OSAKI N,KOYANO T,KOWITHAYAKORN T,et al.Sesquiterpenoids andplasmin-inhibitory flavonoids from Blumea balsamifera[J].J Nat Prod,2005,68(4):447-449.
[3]HASEGAWA H,YAMADA Y,KOMIYAMA K,et al.Dihydroflavonol BB-1,an extract of natural plant Blumea balsamifera,abrogates TRAIL resist-ance in leukemia cells[J].Blood,2006,26(4):679-688.
[4]黄永林,赵志国,文永新.不同部位艾纳香中总黄酮的含量测定[J].广西植物,2006,(26)4:453-455.
[5]朱廷春,文永新,王恒山,等.艾纳香的化学成分研究[J].广西植物,2008,28(1):139-141.
[6]TOSHIO NORIKURA,AKIKO KOJIMA-YUASA,MIKI SHIMIZU,et al.Mechanism of growth inhibitory effect of Blumea balsamifera extract inhepatocellular carcinoma[J].Biosci Biotechnol Biochem,2008,72(5):1183-1189.
[7]安晓娇,康勇,范福洲.壳聚糖用于山茱萸水提液除鞣质工艺的探讨[J].中草药,2008,39(5):706-708.
[8]邹国林,桂兴芬,钟晓凌,等.一种SOD的测活方法——邻苯三酚自氧化法的改进[J].生物化学与生物物理进展,1986,13(4):71-73.
[9]金鸣,蔡亚欣,李金荣,等.邻二氮菲-Fe2+氧化法检测-H2O2/Fe2+产生的羟自由基[J].生物化学与生物物理进展,1996,23(6):553-555.
[10]LEE H C,WEI Y H.Oxidative Stress,Mitochondrial DNA Mutation,andApoptosis in Aging[J].Experimental Biology and Medicine,2007,232:592-606.
[11]AUBERT G,LANSDORP P M.Telomeres and Aging[J].Physiol Rev,2008,88:557-579.
[12]HARMAN D.Free radical theory of aging:an update:increasing the func-tional life span[J].Ann N Y Acad Sci,2006,1067:10-21.
[2]OSAKI N,KOYANO T,KOWITHAYAKORN T,et al.Sesquiterpenoids andplasmin-inhibitory flavonoids from Blumea balsamifera[J].J Nat Prod,2005,68(4):447-449.
[3]HASEGAWA H,YAMADA Y,KOMIYAMA K,et al.Dihydroflavonol BB-1,an extract of natural plant Blumea balsamifera,abrogates TRAIL resist-ance in leukemia cells[J].Blood,2006,26(4):679-688.
[4]黄永林,赵志国,文永新.不同部位艾纳香中总黄酮的含量测定[J].广西植物,2006,(26)4:453-455.
[5]朱廷春,文永新,王恒山,等.艾纳香的化学成分研究[J].广西植物,2008,28(1):139-141.
[6]TOSHIO NORIKURA,AKIKO KOJIMA-YUASA,MIKI SHIMIZU,et al.Mechanism of growth inhibitory effect of Blumea balsamifera extract inhepatocellular carcinoma[J].Biosci Biotechnol Biochem,2008,72(5):1183-1189.
[7]安晓娇,康勇,范福洲.壳聚糖用于山茱萸水提液除鞣质工艺的探讨[J].中草药,2008,39(5):706-708.
[8]邹国林,桂兴芬,钟晓凌,等.一种SOD的测活方法——邻苯三酚自氧化法的改进[J].生物化学与生物物理进展,1986,13(4):71-73.
[9]金鸣,蔡亚欣,李金荣,等.邻二氮菲-Fe2+氧化法检测-H2O2/Fe2+产生的羟自由基[J].生物化学与生物物理进展,1996,23(6):553-555.
[10]LEE H C,WEI Y H.Oxidative Stress,Mitochondrial DNA Mutation,andApoptosis in Aging[J].Experimental Biology and Medicine,2007,232:592-606.
[11]AUBERT G,LANSDORP P M.Telomeres and Aging[J].Physiol Rev,2008,88:557-579.
[12]HARMAN D.Free radical theory of aging:an update:increasing the func-tional life span[J].Ann N Y Acad Sci,2006,1067:10-21.