宫颈鳞癌组织中PDGFR-β蛋白的表达及其意义
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摘要
目的:探讨β型血小板衍生生长因子受体(PDGFR-β)蛋白在宫颈鳞状细胞癌(简称鳞癌)及癌前病变组织中的表达及其临床意义。方法:收集宫颈病变和宫颈鳞癌标本共148例,依据病理诊断结果分为慢性宫颈炎症组、低级别鳞状上皮内病变(LSIL)组、高级别鳞状上皮内病变(HSIL)组及宫颈鳞癌组。采用免疫组化法检测PDGFR-β蛋白在宫颈鳞癌及癌前病变组织中的表达情况,并分析其表达与临床病理特征的相关性。结果:宫颈鳞癌组组织中PDGFR-β蛋白阳性表达率明显高于慢性宫颈炎症组、LSIL组、HSIL组(P<0.05);PDGFR-β蛋白表达与淋巴结转移、TNM分期及癌组织分化程度有关(P<0.05),而与患者年龄无明显相关性(P>0.05)。结论:PDGFR-β在宫颈鳞癌组织中高表达,且与肿瘤转移相关,其可能成为宫颈鳞癌的一个潜在辅助诊断因子及治疗靶点。
Objective: To investigate the expression and clinical significance of platelet derived growth factor receptor-β(PDGFR-β) protein in cervical squamous carcinoma and precancerous tissue. Methods: One hundred and forty-eight tissue cases of cervical lesions and cervical squamous cell carcinomas were collected, and according to pathological diagnosis results, it was divided into chronic cervical inflammation group, low-grade squamous intraepithelial lesion(LSIL) group, high-grade squamous intraepithelial lesion(HSIL) group and cervical squamous cell carcinoma group. The immunohistochemical method was used to detect the expression of PDGFR-β protein in cervical squamous carcinoma and precancerous tissue. Correlation of the expression with the clinical pathological parameters was analyzed. Results: The expression of PDGFR-β protein in cervical squamous cell carcinoma was significantly higher than that in the chronic cervical group, LSIL group and HSIL group(P<0.05). The expression level of PDGFR-β protein in the cervical squamous carcinoma group was related to the TNM stage and lymph node metastasis(P<0.05), but not related to the age(P>0.05). Conclusion: The high expression of PDGFR-β in cervical squamous cell carcinomas is associated with tumor metastasis, which may be a potential auxiliary diagnostic factor and therapeutic target in the treatment of cervical squamous cell carcinoma.
Objective: To investigate the expression and clinical significance of platelet derived growth factor receptor-β(PDGFR-β) protein in cervical squamous carcinoma and precancerous tissue. Methods: One hundred and forty-eight tissue cases of cervical lesions and cervical squamous cell carcinomas were collected, and according to pathological diagnosis results, it was divided into chronic cervical inflammation group, low-grade squamous intraepithelial lesion(LSIL) group, high-grade squamous intraepithelial lesion(HSIL) group and cervical squamous cell carcinoma group. The immunohistochemical method was used to detect the expression of PDGFR-β protein in cervical squamous carcinoma and precancerous tissue. Correlation of the expression with the clinical pathological parameters was analyzed. Results: The expression of PDGFR-β protein in cervical squamous cell carcinoma was significantly higher than that in the chronic cervical group, LSIL group and HSIL group(P<0.05). The expression level of PDGFR-β protein in the cervical squamous carcinoma group was related to the TNM stage and lymph node metastasis(P<0.05), but not related to the age(P>0.05). Conclusion: The high expression of PDGFR-β in cervical squamous cell carcinomas is associated with tumor metastasis, which may be a potential auxiliary diagnostic factor and therapeutic target in the treatment of cervical squamous cell carcinoma.
引文
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[10]KODAMA M,KITADAI Y,SUMIDA T,et al.Expression of platelet- derived growth factor(PDGF)- B and PDGF- receptor β is associated with lymphatic metastasis in human gastric carcinoma[J].Cancer Sci,2010,101(9):1984- 1989.
[11]VERA C,TAPIA V,VEGA M,et al.Role of nerve growth factor and its TRKA receptor in normal ovarian and epithelial ovarian cancer angiogenesis[J].J Ovarian Res,2014,10(7):82- 90.
[12]HELDIN C H,LENNARTSSON J,WESTERMARK B.Involvement of platelet- derived growth factor ligands and receptors in tumorigenesis[J].J Intern Med,2018,283(1):16- 44.
[13]CHEN J,YUAN W,WU L,et al.PDGF- D promotes cell growth,aggressiveness,angiogenesis and EMT transformation of colorectal cancer by activation of Notch1/Twist1 pathway[J].Oncotarget,2017,8(6):9961- 9973.
[14]ABDEL- RAHMAN O.Targeting platelet- derived growth factor(PDGF) signaling in gastrointestinal cancers:preclinical and clinical considerations[J].Tumour Biol,2015,36(1):21- 31.
[2] KIM M,SOHN M,SHIM M,et al.Prognostic value of vascular endothelial growth factor(VEGF),VEGF receptor 2,platelet- derived growth factor- β(PDGF- β),and PDGF- β receptor expression in papillary renal cell carcinoma[J].Hum Pathol,2017,61:78- 89.
[3] 乔立锋.肾透明细胞癌中血小板衍生生长因子BB和微血管密度的检测及临床意义[J].中华肿瘤杂志,2013,35(9):672.
[4] APPIAH- KUBI K,WANG Y,QIAN H.et al.Platelet- derived growth factor receptor/platelet- derived growth factor(PDGFR/PDGF) system is a prognostic and treatment response biomarker with multifarious therapeutic targets in cancers[J].Tumour Biol,2016,37(8):10053- 10066.
[5] GRESSOT L V,DOUCETTE T A,YUHUI Y,et al.Signal transducer and activator of transcription 5b drives malignant progression in a PDGFB- dependent proneural glioma model by suppressing apoptosis[J].Int J Cancer,2015,136(9):2047- 2054.
[6] TIBALDI E,ZONTA F,BORDIN L,et al.The tyrosine phosphatase SHP- 1 inhibits proliferation of activated hepatic stellate cells by impairing PDGF receptor signaling[J].Biochim Biophys Acta,2014,1843(2):288- 298.
[7] 汤伟伟,何永文.PDGF- BB/PDGFR- β与肿瘤发生发展的研究进展[J].临床与病理杂志,2017,37(11):2456- 2462.
[8] 汪晶,宋成文,魏华芳,等.血小板源性生长因子在宫颈癌组织中的表达以及与淋巴管新生的关系[J].华南国防医学杂志,2015,29(8):596- 599.
[9] TUDORAN O M,SORITAU O,BALACESCU L,et al.PDGF beta targeting in cervical cancer cells suggest a fine- tuning of compensatory signalling pathways to sustain tumourigenic stimulation[J].J Cell Mol Med,2015,19(2):371- 382.
[10]KODAMA M,KITADAI Y,SUMIDA T,et al.Expression of platelet- derived growth factor(PDGF)- B and PDGF- receptor β is associated with lymphatic metastasis in human gastric carcinoma[J].Cancer Sci,2010,101(9):1984- 1989.
[11]VERA C,TAPIA V,VEGA M,et al.Role of nerve growth factor and its TRKA receptor in normal ovarian and epithelial ovarian cancer angiogenesis[J].J Ovarian Res,2014,10(7):82- 90.
[12]HELDIN C H,LENNARTSSON J,WESTERMARK B.Involvement of platelet- derived growth factor ligands and receptors in tumorigenesis[J].J Intern Med,2018,283(1):16- 44.
[13]CHEN J,YUAN W,WU L,et al.PDGF- D promotes cell growth,aggressiveness,angiogenesis and EMT transformation of colorectal cancer by activation of Notch1/Twist1 pathway[J].Oncotarget,2017,8(6):9961- 9973.
[14]ABDEL- RAHMAN O.Targeting platelet- derived growth factor(PDGF) signaling in gastrointestinal cancers:preclinical and clinical considerations[J].Tumour Biol,2015,36(1):21- 31.