二子益肾苦荞茶制备工艺及其含量测定
|
摘要
目的:研究二子益肾苦荞茶的制备工艺并对其进行含量测定。方法:以干膏得率与鞣花酸转移率为指标,在单因素试验基础上,采用正交试验考察加水量、提取时间、提取次数对二子益肾苦荞茶中枸杞子、覆盆子提取工艺的影响,对苦荞吸附枸杞子、覆盆子药液比例进行考察,确定二子益肾苦荞茶的制备方法 ;并采用HPLC测定二子益肾苦荞茶中芦丁、鞣花酸的含量。结果:枸杞子和覆盆子的最佳提取工艺为8倍量水,提取3次,每次2 h,出膏率为47.53%,鞣花酸提取率为97.76%;苦荞与枸杞子、覆盆子提取液的吸附比例为1∶0.5时吸附效果最佳;芦丁和鞣花酸的线性关系良好(r>0.999),芦丁和鞣花酸的加样回收率均值分别为103.02%(RSD=1.37%)和102.16%(RSD=1.09%)。结论:该试验研究的最佳提取工艺简单经济;最佳的吸附比例合理,成型工艺可行;该研究建立的含量测定方法简单、准确、灵敏、重复性好,为二子益肾苦荞茶的进一步开发、品质评价和质量控制提供了科学依据。
Objective :To study the optimum preparation process of Erzi Yishen Buckwheat Tea and to determine its content. Methods :The optimum extraction process was determined by orthogonal experiment method with the extraction rate of extract and tannic acid as the index,the optimum molding process was determined by different adsorption ratios,and the content of tea product was determined by HPLC method. Results :The optimum extraction technology of Lycii Fructus and Rubi Fructus was 8 times water,3 times,each time for 2 hours,the extract rate was 47.53%,the extraction rate of tannic acid was 97.76%,the ratio of Tartary buckwheat to Lycii Fructus and Rubi Fructus extract was 1∶0.5,the best adsorption effect was obtainedt. The linear relationship between rutin and tannic acid is good(r>0.999). The average returning rate of rutin and tannic acid were 103.02%(RSD=1.37%)and 102.16%(RSD=1.09%). Conclusion :The optimum extraction technology is simple and economical,the best adsorption ratio is reasonable,and the molding process is feasible. The method for determination the content established in this study is simple,accurate,sensitive and reproducible,which is useful for the further development,quality evaluation and quality control of Erzi Yishen Buckwheat Tea provide scientific basis.
Objective :To study the optimum preparation process of Erzi Yishen Buckwheat Tea and to determine its content. Methods :The optimum extraction process was determined by orthogonal experiment method with the extraction rate of extract and tannic acid as the index,the optimum molding process was determined by different adsorption ratios,and the content of tea product was determined by HPLC method. Results :The optimum extraction technology of Lycii Fructus and Rubi Fructus was 8 times water,3 times,each time for 2 hours,the extract rate was 47.53%,the extraction rate of tannic acid was 97.76%,the ratio of Tartary buckwheat to Lycii Fructus and Rubi Fructus extract was 1∶0.5,the best adsorption effect was obtainedt. The linear relationship between rutin and tannic acid is good(r>0.999). The average returning rate of rutin and tannic acid were 103.02%(RSD=1.37%)and 102.16%(RSD=1.09%). Conclusion :The optimum extraction technology is simple and economical,the best adsorption ratio is reasonable,and the molding process is feasible. The method for determination the content established in this study is simple,accurate,sensitive and reproducible,which is useful for the further development,quality evaluation and quality control of Erzi Yishen Buckwheat Tea provide scientific basis.
引文
[1]张天天,侯芳洁,李英,等.不同产地枸杞子微性状鉴别研究[J].中药材,2016,39(5):1010-1013.
[2]如克亚·加帕尔,孙玉敬,钟烈州,等.枸杞植物化学成分及其生物活性的研究进展[J].中国食品学报,2013,13(8):161-172.
[3]国家药典委员会.中华人民共和国药典(一部)[S].北京:化学工业出版社,2015:249.
[4]王静.枸杞子的药理作用和临床应用价值分析[J].亚太传统医药,2014,10(7):50-51.
[5] CHENG J,ZHOU ZW,SHENG HP,et al. An evidence-based update on the pharmacological activities and possible molecular targets of Lycium barbarum polysaccharides[J]. Drug Des Devel Ther,2014,17(9):33-78.
[6]郭敏,乌兰托雅,李刚.枸杞多糖药理作用的研究进展[J].华西药学杂志,2013,28(6):633-635.
[7]邵鸿娥,刘斌钰,邢雁霞,等.枸杞多糖对小鼠体内抗氧化酶活性及耐力的影响[J].中国自然医学杂志,2010,12(2):133-134.
[8]姜清茹,姚成立,李桂忠.枸杞多糖对高脂血症大鼠血脂及主动脉氧化应激的影响[J].宁夏医学杂志,2010,32(6):504-506.
[9]程丹,李洁,周斌,等.覆盆子化学成分与药理作用研究进展[J].中药材,2012,35(11):1873-1876.
[10]王艳春,李芳,陶蕾.覆盆子提取物对基质金属蛋白酶的体外抑制作用[J].中国临床药理学杂志,2011,27(4):291-292.
[11]牛付阁,王纪平,王芳,等.覆盆子糖蛋白粗提取物体内抗氧化作用研究[J].食品工业科技,2010,31(12):134-136.
[12]田甜,段玉峰,牛付阁.覆盆子糖蛋白的抗氧化作用[J].食品科学,2010,31(21):357-360.
[13]吴峰华,刘相真,杨虎清,等.覆盆子醇提物及其不同极性部位抗氧化活性研究[J].中国食品学报,2012,12(2):24-29.
[14]谢新梅,庞晓斌,李晓婷.覆盆子酮对糖尿病模型小鼠的降血糖作用及其机制研究[J].中国药学杂志,2012,47(23):1899-1904.
[15]樊柏林,龚晨睿,孙凡中,等.湖北掌叶覆盆子叶水提取物降血糖作用动物实验和人群研究[J].食品科学,2010,31(3):239-242.
[16] WANG B,WANG XM,FU H,et al. Protective effects of WuZi-Yan-Zong-Fang on amyloidβ-induced damage in vivo and in vitro[J]. Yakugaku Zasshi,2009,129(8):941-948.
[17]树森,张炳烈,李文彬,等.覆盆子对衰老模型小鼠脑功能的影响[J].中医药学报,1998,26(4):42-43.
[18] Lee M-H,Yang Y-Y,Tsai Y-H,et al. The effect of Chinese herbal medicines on TNF-αinduced matrix metallo proteinase-1,-9 activities and interleukin-8 secretion[J].Botanical Studies,2008,49(4):301-309.
[19]任强.苦荞麦化学成分药理作用及体内代谢研究进展[J].济宁医学院学报,2017,40(4):251-255.
[20]谭平艳,郭皖北.苦荞黄酮的生理功能及其作用机制的研究进展[J].医学综述,2018,24(8):1627-1632.
[2]如克亚·加帕尔,孙玉敬,钟烈州,等.枸杞植物化学成分及其生物活性的研究进展[J].中国食品学报,2013,13(8):161-172.
[3]国家药典委员会.中华人民共和国药典(一部)[S].北京:化学工业出版社,2015:249.
[4]王静.枸杞子的药理作用和临床应用价值分析[J].亚太传统医药,2014,10(7):50-51.
[5] CHENG J,ZHOU ZW,SHENG HP,et al. An evidence-based update on the pharmacological activities and possible molecular targets of Lycium barbarum polysaccharides[J]. Drug Des Devel Ther,2014,17(9):33-78.
[6]郭敏,乌兰托雅,李刚.枸杞多糖药理作用的研究进展[J].华西药学杂志,2013,28(6):633-635.
[7]邵鸿娥,刘斌钰,邢雁霞,等.枸杞多糖对小鼠体内抗氧化酶活性及耐力的影响[J].中国自然医学杂志,2010,12(2):133-134.
[8]姜清茹,姚成立,李桂忠.枸杞多糖对高脂血症大鼠血脂及主动脉氧化应激的影响[J].宁夏医学杂志,2010,32(6):504-506.
[9]程丹,李洁,周斌,等.覆盆子化学成分与药理作用研究进展[J].中药材,2012,35(11):1873-1876.
[10]王艳春,李芳,陶蕾.覆盆子提取物对基质金属蛋白酶的体外抑制作用[J].中国临床药理学杂志,2011,27(4):291-292.
[11]牛付阁,王纪平,王芳,等.覆盆子糖蛋白粗提取物体内抗氧化作用研究[J].食品工业科技,2010,31(12):134-136.
[12]田甜,段玉峰,牛付阁.覆盆子糖蛋白的抗氧化作用[J].食品科学,2010,31(21):357-360.
[13]吴峰华,刘相真,杨虎清,等.覆盆子醇提物及其不同极性部位抗氧化活性研究[J].中国食品学报,2012,12(2):24-29.
[14]谢新梅,庞晓斌,李晓婷.覆盆子酮对糖尿病模型小鼠的降血糖作用及其机制研究[J].中国药学杂志,2012,47(23):1899-1904.
[15]樊柏林,龚晨睿,孙凡中,等.湖北掌叶覆盆子叶水提取物降血糖作用动物实验和人群研究[J].食品科学,2010,31(3):239-242.
[16] WANG B,WANG XM,FU H,et al. Protective effects of WuZi-Yan-Zong-Fang on amyloidβ-induced damage in vivo and in vitro[J]. Yakugaku Zasshi,2009,129(8):941-948.
[17]树森,张炳烈,李文彬,等.覆盆子对衰老模型小鼠脑功能的影响[J].中医药学报,1998,26(4):42-43.
[18] Lee M-H,Yang Y-Y,Tsai Y-H,et al. The effect of Chinese herbal medicines on TNF-αinduced matrix metallo proteinase-1,-9 activities and interleukin-8 secretion[J].Botanical Studies,2008,49(4):301-309.
[19]任强.苦荞麦化学成分药理作用及体内代谢研究进展[J].济宁医学院学报,2017,40(4):251-255.
[20]谭平艳,郭皖北.苦荞黄酮的生理功能及其作用机制的研究进展[J].医学综述,2018,24(8):1627-1632.