A potential germ cell-specific marker in Japanese flounder,Paralichthys olivaceus:identification and characterization of lymphocyte antigen 75(Ly75/CD205)
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摘要
Some germ cell marker genes, such as vasa, nanos, and dead end( dnd), have been identified in fish. Recently, lymphocyte antigen 75(Ly75/CD205) has been identified as a mitotic germ cell-specific cell-surface marker in several fish species. In this study, the Japanese flounder Paralichthys olivaceus ly75 homolog( ly75) was cloned and its expression pattern in gonads was analyzed. The full-length c DNA of ly75 was 7 346 bp, with an open reading frame(ORF) of 5 229 bp. The ORF encoded a protein containing 1 742 amino acids with a predicted molecular mass of 196.89 k Da. In adult tissues, ly75 transcripts were detected in all analyzed tissues but abundantly in the testis. In in-situ hybridization analyses, ly75 mRNA was predominantly localized in oocytes in the ovary and spermatogonia in the testis, but ly75 mRNA was not detected in oogonia, spermatocytes, spermatids, or spermatozoa. These results indicated that ly75 could be a potential germ cell-specific marker in P. olivaceus, as in other fishes.
Some germ cell marker genes, such as vasa, nanos, and dead end( dnd), have been identified in fish. Recently, lymphocyte antigen 75(Ly75/CD205) has been identified as a mitotic germ cell-specific cell-surface marker in several fish species. In this study, the Japanese flounder Paralichthys olivaceus ly75 homolog( ly75) was cloned and its expression pattern in gonads was analyzed. The full-length c DNA of ly75 was 7 346 bp, with an open reading frame(ORF) of 5 229 bp. The ORF encoded a protein containing 1 742 amino acids with a predicted molecular mass of 196.89 k Da. In adult tissues, ly75 transcripts were detected in all analyzed tissues but abundantly in the testis. In in-situ hybridization analyses, ly75 mRNA was predominantly localized in oocytes in the ovary and spermatogonia in the testis, but ly75 mRNA was not detected in oogonia, spermatocytes, spermatids, or spermatozoa. These results indicated that ly75 could be a potential germ cell-specific marker in P. olivaceus, as in other fishes.
Some germ cell marker genes, such as vasa, nanos, and dead end( dnd), have been identified in fish. Recently, lymphocyte antigen 75(Ly75/CD205) has been identified as a mitotic germ cell-specific cell-surface marker in several fish species. In this study, the Japanese flounder Paralichthys olivaceus ly75 homolog( ly75) was cloned and its expression pattern in gonads was analyzed. The full-length c DNA of ly75 was 7 346 bp, with an open reading frame(ORF) of 5 229 bp. The ORF encoded a protein containing 1 742 amino acids with a predicted molecular mass of 196.89 k Da. In adult tissues, ly75 transcripts were detected in all analyzed tissues but abundantly in the testis. In in-situ hybridization analyses, ly75 mRNA was predominantly localized in oocytes in the ovary and spermatogonia in the testis, but ly75 mRNA was not detected in oogonia, spermatocytes, spermatids, or spermatozoa. These results indicated that ly75 could be a potential germ cell-specific marker in P. olivaceus, as in other fishes.
引文
East L,Isacke C M.2002.The mannose receptor family.Biochimica et Biophysica Acta(BBA)-General Subjects,1572(2-3):364-386.
Fan Z F,You F,Wang L J,Weng S D,Wu Z H,Hu J W,Zou YX,Tan X G,Zhang P J.2014.Gonadal transcriptome analysis of male and female olive flounder(Paralichthys olivaceus).BioMed Research International,2014:291067.
Huang J Q,Li Y J,Shao C W,Wang N,Chen S L.2017.Identification,characterization and functional analysis of regulatory region of nanos gene from half-smooth tongue sole(Cynoglossus semilaevis).Gene,617:8-16.
Jiang W P,Swiggard W J,Heufler C,Peng M,Mirza A,Steinman R M,Nussenzweig M C.1995.The receptor DEC-205 expressed by dendritic cells and thymic epithelial cells is involved in antigen processing.Nature,375(6527):151-155.
Kagawa H.2013.Oogenesis in teleost fish.Aqua-BioScience Monographs(ABSM),6(4):99-127.
Kurokawa H,Saito D,Nakamura S,Katoh-Fukui Y,Ohta K,Baba T,Morohashi K,Tanaka M.2007.Germ cells are essential for sexual dimorphism in the medaka gonad.Proceedings of the National Academy of Sciences of the United States of America,104(43):16 958-16 963.
Lacerda S M S N,Aponte P M,Costa G M J,Campos-Junior PH A,Segatelli T M,Silva M A,Franca L R.2012.An overview of spermatogonial stem cell physiology,niche and transplantation in fish.Animal Reproduction,9(4):798-808.
Li M J,Tan X G,Jiao S,Wang Q,Wu Z H,You F,Zou Y X.2015.A new pattern of primordial germ cell migration in olive flounder(Paralichthys olivaceus)identified using nanos3.Development Genes and Evolution,225(4):195-206.
Nagasawa K,Fernandes J M O,Yoshizaki G,Miwa M,Babiak I.2013.Identification and migration of primordial germ cells in Atlantic salmon,Salmo salar:characterization of Vasa,Dead End,and Lymphocyte antigen 75 genes.Molecular Reproduction and Development,80(2):118-131.
Nagasawa K,Miwa M,Yazawa R,Morita T,Takeuchi Y,Yoshizaki G.2012.Characterization of lymphocyte antigen 75(Ly75/CD205)as a potential cell-surface marker on spermatogonia in Pacific bluefin tuna Thunnus orientalis.Fisheries Science,78(4):791-800.
Nagasawa K,Shikina S,Takeuchi Y,Yoshizaki G.2010.Lymphocyte antigen 75(Ly75/CD205)is a surface marker on mitotic germ cells in rainbow trout.Biology of Reproduction,83(4):597-606.
Nakamura S,Kobayashi K,Nishimura T,Tanaka M.2011.Ovarian germline stem cells in the teleost fish,medaka(Oryzias latipes).International Journal of Biological Sciences,7(4):403-409.
Presslauer C,Nagasawa K,Dahle D,Babiak J,Fernandes J MO,Babiak I.2014.Induced autoimmunity against gonadal proteins affects gonadal development in juvenile zebrafish.PLoS One,9(12):e114209,https://doi.org/10.1371/journal.pone.0114209.
Radonic M,Macchi G J.2009.Gonadal sex differentiation in cultured juvenile flounder,Paralichthys orbignyanus(Valenciennes,1839).Journal of the World Aquaculture Society,40(1):129-133.
Schulz R W,de Fran L R,Lareyre J J,Le Gac F,ChiariniGarcia H,Nobrega R H,Miura T.2010.Spermatogenesis in fish.General and Comparative Endocrinology,165(3):390-411.
Si Y F,Ding Y X,He F,Wen H S,Li J F,Zhao J L,Huang Z J.2016.DNA methylation level of cyp19a1a and Foxl2 gene related to their expression patterns and reproduction traits during ovary development stages of Japanese flounder(Paralichthys olivaceus).Gene,575(2):321-330.
Suzuki A,Tsuda M,Saga Y.2007.Functional redundancy among Nanos proteins and a distinct role of Nanos2during male germ cell development.Development,134(1):77-83.
Wang L J,You F,Weng S D,Wen A Y,Wu Z H,Zou Y X,Xin M J,Zhang P J.2015a.Molecular cloning and sexually dimorphic expression patterns of nr0b1 and nr5a2 in olive flounder,Paralichthys olivaceus.Development Genes and Evolution,225(2):95-104.
Wang P,Jiang C Y,Liu S S,Cui P F,Zhang Y,Zhang S C.2017.Trans-generational enhancement of C-type lysozyme level in eggs of zebrafish by dietaryβ-glucan.Developmental&Comparative Immunology,74:25-31.
Wang X Y,Liu Q H,Xiao Y S,Yang Y,Wang Y F,Song Z C,You F,An H,Xiao Z Z,Xu S H,Ma D Y,Li J.2015b.The dnd RNA identifies germ cell origin and migration in olive flounder(Paralichthys olivaceus).BioMed Research International,2015:428591.
Zhang S C,Wang Z P,Wang H M.2013.Maternal immunity in fish.Developmental&Comparative Immunology,39(1-2):72-78.
Fan Z F,You F,Wang L J,Weng S D,Wu Z H,Hu J W,Zou YX,Tan X G,Zhang P J.2014.Gonadal transcriptome analysis of male and female olive flounder(Paralichthys olivaceus).BioMed Research International,2014:291067.
Huang J Q,Li Y J,Shao C W,Wang N,Chen S L.2017.Identification,characterization and functional analysis of regulatory region of nanos gene from half-smooth tongue sole(Cynoglossus semilaevis).Gene,617:8-16.
Jiang W P,Swiggard W J,Heufler C,Peng M,Mirza A,Steinman R M,Nussenzweig M C.1995.The receptor DEC-205 expressed by dendritic cells and thymic epithelial cells is involved in antigen processing.Nature,375(6527):151-155.
Kagawa H.2013.Oogenesis in teleost fish.Aqua-BioScience Monographs(ABSM),6(4):99-127.
Kurokawa H,Saito D,Nakamura S,Katoh-Fukui Y,Ohta K,Baba T,Morohashi K,Tanaka M.2007.Germ cells are essential for sexual dimorphism in the medaka gonad.Proceedings of the National Academy of Sciences of the United States of America,104(43):16 958-16 963.
Lacerda S M S N,Aponte P M,Costa G M J,Campos-Junior PH A,Segatelli T M,Silva M A,Franca L R.2012.An overview of spermatogonial stem cell physiology,niche and transplantation in fish.Animal Reproduction,9(4):798-808.
Li M J,Tan X G,Jiao S,Wang Q,Wu Z H,You F,Zou Y X.2015.A new pattern of primordial germ cell migration in olive flounder(Paralichthys olivaceus)identified using nanos3.Development Genes and Evolution,225(4):195-206.
Nagasawa K,Fernandes J M O,Yoshizaki G,Miwa M,Babiak I.2013.Identification and migration of primordial germ cells in Atlantic salmon,Salmo salar:characterization of Vasa,Dead End,and Lymphocyte antigen 75 genes.Molecular Reproduction and Development,80(2):118-131.
Nagasawa K,Miwa M,Yazawa R,Morita T,Takeuchi Y,Yoshizaki G.2012.Characterization of lymphocyte antigen 75(Ly75/CD205)as a potential cell-surface marker on spermatogonia in Pacific bluefin tuna Thunnus orientalis.Fisheries Science,78(4):791-800.
Nagasawa K,Shikina S,Takeuchi Y,Yoshizaki G.2010.Lymphocyte antigen 75(Ly75/CD205)is a surface marker on mitotic germ cells in rainbow trout.Biology of Reproduction,83(4):597-606.
Nakamura S,Kobayashi K,Nishimura T,Tanaka M.2011.Ovarian germline stem cells in the teleost fish,medaka(Oryzias latipes).International Journal of Biological Sciences,7(4):403-409.
Presslauer C,Nagasawa K,Dahle D,Babiak J,Fernandes J MO,Babiak I.2014.Induced autoimmunity against gonadal proteins affects gonadal development in juvenile zebrafish.PLoS One,9(12):e114209,https://doi.org/10.1371/journal.pone.0114209.
Radonic M,Macchi G J.2009.Gonadal sex differentiation in cultured juvenile flounder,Paralichthys orbignyanus(Valenciennes,1839).Journal of the World Aquaculture Society,40(1):129-133.
Schulz R W,de Fran L R,Lareyre J J,Le Gac F,ChiariniGarcia H,Nobrega R H,Miura T.2010.Spermatogenesis in fish.General and Comparative Endocrinology,165(3):390-411.
Si Y F,Ding Y X,He F,Wen H S,Li J F,Zhao J L,Huang Z J.2016.DNA methylation level of cyp19a1a and Foxl2 gene related to their expression patterns and reproduction traits during ovary development stages of Japanese flounder(Paralichthys olivaceus).Gene,575(2):321-330.
Suzuki A,Tsuda M,Saga Y.2007.Functional redundancy among Nanos proteins and a distinct role of Nanos2during male germ cell development.Development,134(1):77-83.
Wang L J,You F,Weng S D,Wen A Y,Wu Z H,Zou Y X,Xin M J,Zhang P J.2015a.Molecular cloning and sexually dimorphic expression patterns of nr0b1 and nr5a2 in olive flounder,Paralichthys olivaceus.Development Genes and Evolution,225(2):95-104.
Wang P,Jiang C Y,Liu S S,Cui P F,Zhang Y,Zhang S C.2017.Trans-generational enhancement of C-type lysozyme level in eggs of zebrafish by dietaryβ-glucan.Developmental&Comparative Immunology,74:25-31.
Wang X Y,Liu Q H,Xiao Y S,Yang Y,Wang Y F,Song Z C,You F,An H,Xiao Z Z,Xu S H,Ma D Y,Li J.2015b.The dnd RNA identifies germ cell origin and migration in olive flounder(Paralichthys olivaceus).BioMed Research International,2015:428591.
Zhang S C,Wang Z P,Wang H M.2013.Maternal immunity in fish.Developmental&Comparative Immunology,39(1-2):72-78.