多脂长喙壳菌次生代谢产物的抑菌活性研究
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摘要
植物病原真菌是生态系统的重要组成部分,能够代谢产生多种生物活性物质。用纸片法检测多脂长喙壳菌(Ceratocystis adiposa)发酵提取物对13种致病细菌的抑菌活性,并对潜在抗菌活性化合物的热、酸碱和光稳定性进行检测,同时应用OSMAC策略寻找多脂长喙壳菌产抗菌化合物的最佳培养条件。结果表明:多脂长喙壳菌提取物对革兰阳性菌——蜡样芽胞杆菌(Bacillus cereus)、缓慢芽胞杆菌(Bacillus lentus)、藤黄微球菌(Micrococcus luteus)具有抗菌活性,MIC分别为6.25、3.125和1.562 5 mg/m L,抗菌化合物具有较好的耐热、耐酸碱和耐辐射性,培养基种类、培养时间和接种量会影响多脂长喙壳菌抗菌化合物的产生,为多脂长喙壳菌抗菌化合物的开发利用提供参考。
Plant pathogenic fungi are important parts of the ecosystem,they are able to metabolize a variety of bioactive substances. In this study,the antibacterial activity of Ceratocystis adiposa fermented extract on 13 kinds of pathogenic bacteria was screened adopting paper method. The heat, acid-base and light stability of potential bacteriostatic active compounds were tested. Meanwhile,OSMAC strategy was used to find the optimum culture conditions for the C. adiposa production of antibacterial compounds. The results showed that C. adiposa extract have antibacterial activity against gram-positive bacteria,Bacillus cereus,Bacillus lentus,and Micrococcus luteus,and the MIC values of C. adiposa extract were 6. 25,3. 125,1. 562 5 mg/m L respectively. Antibacterial compounds possessed heat resistance,acid-base resistance and radiation resistance. The type of media, culture time and inoculum amount could affect the production of antibacterial compounds by C. adiposa. This study has laid the foundation for the development and utilization of antibacterial compounds by C. adiposa.
Plant pathogenic fungi are important parts of the ecosystem,they are able to metabolize a variety of bioactive substances. In this study,the antibacterial activity of Ceratocystis adiposa fermented extract on 13 kinds of pathogenic bacteria was screened adopting paper method. The heat, acid-base and light stability of potential bacteriostatic active compounds were tested. Meanwhile,OSMAC strategy was used to find the optimum culture conditions for the C. adiposa production of antibacterial compounds. The results showed that C. adiposa extract have antibacterial activity against gram-positive bacteria,Bacillus cereus,Bacillus lentus,and Micrococcus luteus,and the MIC values of C. adiposa extract were 6. 25,3. 125,1. 562 5 mg/m L respectively. Antibacterial compounds possessed heat resistance,acid-base resistance and radiation resistance. The type of media, culture time and inoculum amount could affect the production of antibacterial compounds by C. adiposa. This study has laid the foundation for the development and utilization of antibacterial compounds by C. adiposa.
引文
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[23]Lien HM,Tseng CJ,Huang CL,et al.Antimicrobial activity of Antrodia camphorata extracts against oral bacteria[J].Plos One,2013,9(8):e105286-e105286.
[24]Bode HB,Bethe B,H9fs R,et al.Big Effects from Small Changes:Possible Ways to Explore Nature's Chemical Diversity[J].Chembiochem,2015,3(7):619-627.
[25]卢轩,陈刚,裴月湖.单菌多产物策略及其在药物先导化合物研发中的应用[J].沈阳药科大学学报,2011,(8):640-645.
[26]Nielsen ML,Nielsen JB,Rank C,et al.A genome-wide polyketide synthase deletion library uncovers novel genetic links to polyketides and meroterpenoids in Aspergillus nidulans[J].Fems Microbiology Letters,2011,321(2):157-166.
[27]肖支叶,原晓龙,华梅,等.Leptographium bhutanense提取物抑菌活性的初步研究[J].基因组学与应用生物学,2018,37(2):808-814.
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[29]康子腾,姜黎明,罗义勇,等.植物病原链格孢属真菌的致病机制研究进展[J].生命科学,2013,(9):908-914.
[30]李川,刘海英,范永山,等.植物病原真菌群体遗传学研究进展[J].河北农业大学学报,2003,26(z1):177-179.
[31]向梅梅.植物病原真菌分子生物学研究进展[J].仲恺农业工程学院学报,2001,14(4):52-58.
[2]赵桂华.长喙壳属的两个新种[J].南京林业大学学报,1992,16(2):81-86.
[3]Bramorski A,Soccol CR,Christen P,et al.Fruity aroma production by Ceratocystis fimbriata in solid cultures from agroindustrial wastes[J].Revista de Microbiologia,1998,29(3):208-212.
[4]Soares M,Christen P,Pandey A,et al.Fruity flavour production by Ceratocystis fimbriata grown on coffee husk in solid-state fermentation[J].Process Biochemistry,2000,35(8):857-861.
[5]Christen P,Rainbault M.Optimization of culture medium for aroma production by Ceratocystis fimbriata[J]Biotechnology Letters,1991,13(7):521-526.
[6]Christen P,Villegas E,Revah S.Growth and aroma production by Ceratocystis fimbriata in various fermentation media[J].Biotechnology Letters,1994,16(11):1183-1188.
[7]Sánchez L,Sánchez F,Christen P,et al.Effects of water activity,leucine and thiamine on production of aroma compounds by Ceratocystis fimbriata ta[J].World Journal of Microbiology and Biotechnology,2004,20(2):151-160.
[8]Sanchez L,Seila RL,Christen P,et al.Effect of leucine on aroma volatiles production from Ceratocystis fimbriata grown in liquid culture.[J].World Journal of Microbiology and Biotechnology,2002,18(3):231-238.
[9]李倩,邓吉,李健强.甘薯长喙壳菌产生芳香性气体物质研究进展[J].植物保护,2009,35(4):8-14.
[10]李婕,李永川,杨虹,等.甘蔗黑腐病病原菌的鉴定[J].云南大学学报自然科学版,2014,36(1):139-143.
[11]Strobel GA.The story of mycodiesel[J].Current Opinion in Microbiology,2014,19:52-58.
[12]Morath SU,Hung R,Bennett JW.Fungal volatile organic compounds:a review with emphasis on their biotechnological potential[J].Fungal Biology Reviews,2012,26(2-3):73-83.
[13]陈利军,史洪中,陈月华,等.具有药用价值的植物病原真菌[J].时珍国医国药,2007,18(3):695-697.
[14]Arnold AE.Understanding the diversity of foliar endophytic fungi:progress,challenges,and frontiers[J].Fungal Biology Reviews,2007,21(2-3):51-66.
[15]Zhao J,Shan T,Mou Y,et al.Plant-derived bioactive compounds produced by endophytic fungi[J].Mini Reviews in Medicinal Chemistry,2011,11(2):159-68.
[16]Kusari S,Pandey SP,Spiteller M.Untapped mutualistic paradigms linking host plant and endophytic fungal production of similar bioactive secondary metabolites[J].Phytochemistry,2013,91(4):81.
[17]戈惠明,谭仁祥.共生菌—新活性天然产物的重要来源[J].化学进展,2009,21(1):30-46.
[18]Bates I,Rowland S.Penicillin:from discovery to product.[J].Bulletin of the World Health Organization,2001,79(8):778-779.
[19]Chain E,Florey HW,Jennings MA,et al.Helvolic Acid,an Antibiotic Produced by Aspergillus fumigatus,mut.helvola Yuill[J].British Journal of Experimental Pathology,1943,24(3):108-119.
[20]Strobel G,Stierle A,Stierle D.Taxol and taxane production by Taxomycetes andreanae an endophytic fungus of Pacific rew[J].Science,1993,260(5105):214-216.
[21]Wang QX,Li B,Yang XL.Polyketides with antimicrobial activity from the solid culture of an endolichenic fungus Ulocladium sp.[J].Fitoterapia,2012,(83):209-214.
[22]赵能,原晓龙,华梅,等.地衣型真菌次级代谢产物抗菌活性初步研究[J].广西植物,2017,37(2):242-247.
[23]Lien HM,Tseng CJ,Huang CL,et al.Antimicrobial activity of Antrodia camphorata extracts against oral bacteria[J].Plos One,2013,9(8):e105286-e105286.
[24]Bode HB,Bethe B,H9fs R,et al.Big Effects from Small Changes:Possible Ways to Explore Nature's Chemical Diversity[J].Chembiochem,2015,3(7):619-627.
[25]卢轩,陈刚,裴月湖.单菌多产物策略及其在药物先导化合物研发中的应用[J].沈阳药科大学学报,2011,(8):640-645.
[26]Nielsen ML,Nielsen JB,Rank C,et al.A genome-wide polyketide synthase deletion library uncovers novel genetic links to polyketides and meroterpenoids in Aspergillus nidulans[J].Fems Microbiology Letters,2011,321(2):157-166.
[27]肖支叶,原晓龙,华梅,等.Leptographium bhutanense提取物抑菌活性的初步研究[J].基因组学与应用生物学,2018,37(2):808-814.
[28]杨斌,叶建仁,包宏.林木病原菌毒素研究进展[J].林业科学研究,2000,13(3):316-322.
[29]康子腾,姜黎明,罗义勇,等.植物病原链格孢属真菌的致病机制研究进展[J].生命科学,2013,(9):908-914.
[30]李川,刘海英,范永山,等.植物病原真菌群体遗传学研究进展[J].河北农业大学学报,2003,26(z1):177-179.
[31]向梅梅.植物病原真菌分子生物学研究进展[J].仲恺农业工程学院学报,2001,14(4):52-58.