球孢白僵菌发酵液抗人体致病细菌活性研究
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摘要
为了探究球孢白僵菌YFCCFQ001发酵液的抗菌活性以及不同培养基对其抗菌效果的影响,用乙酸乙酯萃取球孢白僵菌YFCCFQ001发酵液,旋干后得到提取物,采用抑菌圈法测定其对6种耐药性人体致病细菌以及8种普通人体致病细菌的抗菌活性,并通过单菌多产物策略,改变培养基碳氮源,检测抗菌效果的变化。结果表明:球孢白僵菌YFCCFQ001提取物对4种耐药性致病细菌(溶血性葡萄球菌、大肠埃希菌、粪肠球菌、金黄色葡萄球菌)和8种普通致病细菌均有抗菌活性(其中关于球孢白僵菌对副溶血性弧菌、短小芽孢杆菌、无乳链球菌、福氏志贺氏菌、乙型副伤寒沙门氏菌的抗菌活性未见文献报道)。不同氮源对其抗菌活性影响显著,其中胰蛋白胨抗菌效果最好,对3种耐药性致病细菌和6种普通致病细菌均有活性。研究发现了球孢白僵菌对耐药性致病细菌的抗菌活性,以及不同培养基对球孢白僵菌发酵液抗菌活性的影响,为真菌中新型抗菌天然产物的寻找和利用提供新的思路。
In order to explore the antibacterial activity of extracts of Beauveria bassiana(YFCCFQ001) and effect of different medium on antibacterial activity,inoculating inhibition zone was used to evaluate the antimicrobial activity of ethyl acetate extracts of B.bassiana against 6 resistant pathogenic bacteria and 8 common human pathogenic bacteria,and one strain-many compounds(OSMAC) strategy was adopted to evaluate the effect of different carbon sources,nitrogen sources and inoculation on antibacterial activity.The results showed that the extracts of B.bassiana had significant antibacterial activity against 4 resistant pathogenic bacteria(Straphylococcus haemolyticus,Staphylococcus aureus,Enterococcus faecalis,Escherichia coli) and 8 common pathogenic bacteria(Beauveria bassiana against Vibrio parahaemolyticus,Bacillus pumilus,Streptcococcus agalactiae,Shigella flexneri,Salmonella paratyphi were the firstly reported).Different nitrogen sources had significant effects on antibacterial activity,and the extracts from tryptone had the best antibacterial effect that against 3 resistant pathogenic bacteria and 6 common pathogenic bacteria.The antibacterial activity of B.bassiana against resistant pathogenic bacteria and the effect of different medium on antibacterial activity was found,and it is beneficial to the development of novel antimicrobial compounds against human pathogenic bacteria.
In order to explore the antibacterial activity of extracts of Beauveria bassiana(YFCCFQ001) and effect of different medium on antibacterial activity,inoculating inhibition zone was used to evaluate the antimicrobial activity of ethyl acetate extracts of B.bassiana against 6 resistant pathogenic bacteria and 8 common human pathogenic bacteria,and one strain-many compounds(OSMAC) strategy was adopted to evaluate the effect of different carbon sources,nitrogen sources and inoculation on antibacterial activity.The results showed that the extracts of B.bassiana had significant antibacterial activity against 4 resistant pathogenic bacteria(Straphylococcus haemolyticus,Staphylococcus aureus,Enterococcus faecalis,Escherichia coli) and 8 common pathogenic bacteria(Beauveria bassiana against Vibrio parahaemolyticus,Bacillus pumilus,Streptcococcus agalactiae,Shigella flexneri,Salmonella paratyphi were the firstly reported).Different nitrogen sources had significant effects on antibacterial activity,and the extracts from tryptone had the best antibacterial effect that against 3 resistant pathogenic bacteria and 6 common pathogenic bacteria.The antibacterial activity of B.bassiana against resistant pathogenic bacteria and the effect of different medium on antibacterial activity was found,and it is beneficial to the development of novel antimicrobial compounds against human pathogenic bacteria.
引文
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[19]林淑婷,李从发,胡晓苹,等.不同发酵方式对海南粗榧内生真菌CH1307c合成次级代谢产物的影响[J].热带作物学报,2016,37(7):1407-1412.
[2]Wang W X,Kusari S,Laatsch H, et al.Antibacterial Azaphilones from an Endophytic Fungus, Colletotrichum sp.BS4[J].Journal of Natural Products,2016,79(4):704-710.
[3]华梅,王垚,肖支叶,等.贵州绿僵菌液体培养提取物抑菌活性研究[J].西南林业大学学报(自然科学版),2018,38(2):134-140.
[4]刘鑫,侯若琳,刘建兵,等.球孢白僵菌深层发酵液抗乳腺癌活性部位筛选及成分分析[J].菌物学报,2017(12):1659-1666.
[5]Lin H I,Lee Y J,Chen B F, et al.Involvement of Bcl-2 family,cytochrome c,and caspase 3 in induction of apoptosis by beauvericin in human non-small cell lung cancer cells[J].Cancer Letters,2005,230(2):248-259.
[6]Zhan J,Burns A M,Liu M X, et al.Search for cell motility and angiogenesis inhibitors with potential anticancer activity:Beauvericin and other constituents of two endophytic strains of Fusarium oxysporum[J].Journal of Natural Products,2007,70(2):227-232.
[7]Jow G M,Chou C J,Chen B F, et al.Beauvericin induces cytotoxic effects in human acute lymphoblastic leukemia cells through cytochrome c,release,caspase 3 activation:the causative role of calcium[J].Cancer Letters,2004,216(2):165-173.
[8]Shin C G,An D G,Song H H, et al.Beauvericin and enniatins H,I and MK1688 are new potent inhibitors of human immunodeficiency virus type-1 integrase[J].Journal of Antibiotics,2009,62(12):687-690.
[9]Nilanonta C,Isaka M,Kittakoop P, et al.Antimycobacterial and antiplasmodial cyclodepsipeptides from the insect pathogenic fungus Paecilomyces tenuipes BCC 1614[J].Planta Medica,2000,66(08):756-758.
[10]Castlebury L A,Sutherland J B,Tanner L A, et al.Short communication:use of a bioassay to evaluate the toxicity of beauvericin to bacteria.[J].World Journal of Microbiology & Biotechnology,1999,15(1):119-121.
[11]Meca G,Sospedra I,Soriano J M, et al.Antibacterial effect of the bioactive compound beauvericin produced by Fusarium proliferatum on solid medium of wheat.[J].Toxicon,2010,56(3):349-354.
[12]Dzoyem J P,Melong R,Tsamo A T, et al.Cytotoxicity,antioxidant and antibacterial activity of four compounds produced by an endophytic fungus Epicoccum nigrum,associated with Entada abyssinica[J].Revista Brasileira De Farmacognosia,2017,27:251-253.
[13]Xu L,Wang J,Zhao J, et al.Beauvericin from the endophytic fungus, Fusarium redolens,isolated from Dioscorea zingiberensis and its antibacterial activity[J].Natural Product Communications,2010,5:811-814.
[14]卢轩,陈刚,裴月湖.单菌多产物策略及其在药物先导化合物研发中的应用[J].沈阳药科大学学报,2011,28(8):640-645.
[15]Fang X,Yang B,Cheng Z, et al.Synthesis and antimicrobial activity of novel chalcone derivatives[J].Res ChemInterm,2014,40(4):1715-1725.
[16]党悦方,田娇,尚娇,等.太白山4种药用地衣抑菌活性研究[J].西北大学学报(自然科学版),2014,44(3):433-438.
[17]赵敏,谭钢文,唐小异,等.十种中药提取物与抗生素合用对铜绿假单胞菌最小抑菌浓度影响的体外实验[J].湖南师范大学学报(医学版),2011,8(4):80-83.
[18]韦洪娟,林贞建,李德海,等.单菌多次级代谢产物方法及其在微生物代谢产物研究中的应用[J].微生物学报,2010,50(6):701-709.
[19]林淑婷,李从发,胡晓苹,等.不同发酵方式对海南粗榧内生真菌CH1307c合成次级代谢产物的影响[J].热带作物学报,2016,37(7):1407-1412.