不同血液保存方式对藏、汉族人群红细胞携/释氧功能的影响
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摘要
目的评价藏、汉族人群红细胞在不同保存条件下携/释氧功能的变化。方法收集世居高原藏族7份、世居平原汉族6份,上述两类健康成年人血液标本。采集上述两类人群抗凝血,利用不同保存液(4℃EDTA抗凝剂保存、4℃添加CPDA-1红细胞保存液、复方甘油溶液–80℃)保存红细胞,3d后检测上述不同保存条件下的红细胞P_(50)值;另检测4℃EDTA抗凝剂保存样本的pH值,分析可能影响P_(50)的因素。结果藏、汉族所有保存3d后血液标本P_(50)值均较正常人P_(50)值有不同程度的下降。不同保存条件对藏、汉族人群P_(50)均有影响,两组人群在保存液中的红细胞P_(50)均高于4℃EDTA保存(P <0.05)和复方甘油溶液–80℃冻存样品(P <0.05)的P_(50)值。复方甘油溶液–80℃冻存样品与4℃EDTA保存样品的P_(50)值相当(P>0.05)。但同一保存条件下,高原藏族人群与平原汉族人群P_(50)值的差异无统计学意义,高原藏族人群4℃添加保存液的红细胞P_(50)检测值稍高于平原人群,但差异无统计学意义(P>0.05)。高原藏族、平原汉族人群4℃EDTA保存血样pH值相均偏酸性。结论不同保存方式对红细胞P_(50)产生显著影响。三种不同保存液对藏、汉族人群红细胞P_(50)的影响一致。两类人群在CPDA-1保存液中保存的红细胞P_(50)值均相对较高,该保存液对其携放氧影响较小。上述结论将对我国血液条件下保存及藏、汉族患者血液输注品种选择,尤其是高原藏族人群输血提供一定的指导意义。
Objective To evaluate the effects of the populations of the Tibetan and Han Plain on the function of red blood cells carrying oxygen in different storage conditions.Methods Anticoagulation was collected from the populations of 7 the Tibetan and 6 Han Plain and red blood cells were saved with different preservation solutions(4℃ EDTA anticoagulant,4℃ red blood cell preservation solution,compound glycerol solution –80℃).After three days red blood cell P_(50) was detected under the above conditions.The pH of the 4℃ EDTA anticoagulant was also detected for analyzing the reasons that may affect P_(50).Results The P_(50) datas of all specimens of Tibetan and Han nationality were lower than those of normal people.The P_(50) data in the preservation solution at 4℃ was higher than those in the 4℃ EDTA anticoagulant and whole blood of the frozen solution which was stored in glycerol solution-80℃.The P_(50) data of the red cell of the Tibetan stored in the preservation solution at 4℃ was higher than that of Han Plain.At the same time,there is no significantly difference in the P_(50) data of whole blood which was stored at 4℃ or–80℃ Conclusion Different preservation solutions could have significant effect on P_(50) data of red blood cells.There is no difference between the three different preservation solutions on the P_(50) of Tibetan an Han population.The P_(50) of red blood cells stored in the CAPD-1 preservation sloution of the two groups were relatively high,and the preservation solution had little effect on the red blood cell carrying oxygen.Therefore the above conclusions will provide guiding significance for blood preservation and transfusion of patients,especially for high altitude blood transfusion.
Objective To evaluate the effects of the populations of the Tibetan and Han Plain on the function of red blood cells carrying oxygen in different storage conditions.Methods Anticoagulation was collected from the populations of 7 the Tibetan and 6 Han Plain and red blood cells were saved with different preservation solutions(4℃ EDTA anticoagulant,4℃ red blood cell preservation solution,compound glycerol solution –80℃).After three days red blood cell P_(50) was detected under the above conditions.The pH of the 4℃ EDTA anticoagulant was also detected for analyzing the reasons that may affect P_(50).Results The P_(50) datas of all specimens of Tibetan and Han nationality were lower than those of normal people.The P_(50) data in the preservation solution at 4℃ was higher than those in the 4℃ EDTA anticoagulant and whole blood of the frozen solution which was stored in glycerol solution-80℃.The P_(50) data of the red cell of the Tibetan stored in the preservation solution at 4℃ was higher than that of Han Plain.At the same time,there is no significantly difference in the P_(50) data of whole blood which was stored at 4℃ or–80℃ Conclusion Different preservation solutions could have significant effect on P_(50) data of red blood cells.There is no difference between the three different preservation solutions on the P_(50) of Tibetan an Han population.The P_(50) of red blood cells stored in the CAPD-1 preservation sloution of the two groups were relatively high,and the preservation solution had little effect on the red blood cell carrying oxygen.Therefore the above conclusions will provide guiding significance for blood preservation and transfusion of patients,especially for high altitude blood transfusion.
引文
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7 Li C,Li X,Liu J,et al.Investigation of the differences between the Tibetan and Han populations in the hemoglobin-oxygen affinity of red blood cells and in the adaptation to high-altitude environments[J],Hematology,2018,23(5):309-313.
8李小薇,李翠莹.藏、汉族中遗传差异与高原低氧适应研究[J].中国输血杂志,2017,30(8):877-879.
2董鹏,李修良,车辑,等.术中自体回收洗涤红细胞携氧能力评价[J].中国输血杂志,2016,29(3):235-238.
3兰珂,王翔.失血性休克过程中红细胞携氧-释氧动力学研究[J].生物医学工程学杂志,2012,29(4):111-114.
4燕备战,马会敏,孔存权,等.不同保存时间的红细胞携氧能力和体内存活变化研究[J].中国卫生检验杂志,2017,27(12):1721-1724.
5王红,贺曾,吴瑕,等.滤除白细胞对全血保存质量的影响[J].中国输血杂志,2013,26(6):525-529.
6刘娟,李小薇,赵莲,等.高原低压低氧环境对红细胞携放氧的影响[J].中国输血杂志,2017,30(8):872-873.
7 Li C,Li X,Liu J,et al.Investigation of the differences between the Tibetan and Han populations in the hemoglobin-oxygen affinity of red blood cells and in the adaptation to high-altitude environments[J],Hematology,2018,23(5):309-313.
8李小薇,李翠莹.藏、汉族中遗传差异与高原低氧适应研究[J].中国输血杂志,2017,30(8):877-879.