响应面法优化超高压提取黄精多糖工艺
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摘要
以黄精为研究对象,采用超高压提取技术,利用单因素实验及响应面方法优化了超高压提取黄精多糖的工艺条件,以期获得较优的工艺参数,并将实验结果与传统工艺进行对比;对黄精多糖的抗氧化性进行了考察。结果表明,超高压提取黄精多糖在压力255 MPa、物料粒径40目、固液比1∶17(固指物料质量,g;液指提取剂体积,m L)、保压时间9.5 min、提取温度为常温、提取剂为水的工艺条件下,多糖提取率为25.01%;而传统煎煮法在提取时间90 min、提取温度100℃的工艺条件下,多糖提取率为19.83%,表明超高压提取黄精多糖方式较为有效。当黄精多糖质量浓度为1 g/L时,其对1,1-二苯基-2-三硝基苯肼(DPPH)自由基的清除能力达到34.14%。
The ultrahigh pressure extraction technology was used to extract polysaccharides from Polygonatum cyrtonema Hua. The single factor experiment and response surface method were applied to obtain optimal process parameters. Furthermore, the antioxidant activity of Polygonatum polysaccharides was investigated.The results showed that the optimal extraction conditions were as follows: pressure 255 MPa, particle size of raw material 40 mesh, ratio of raw material to extraction agent 1∶17(solids refers the mass of the raw material, g; liquid refers to the volume of the extractant agent, mL), extraction time 9.5 min, extraction temperature room temperature,and extractant agent(water). Under these conditions, the extraction rate of polysaccharides was 25.01%. While the traditional decocting method had an extraction rate of polysaccharides of 19.83% at 100 ℃ for 90 min, which indicated that ultrahigh pressure extraction technology was more effective technology for extracting Polygonatum polysaccharides. The scavenging ability of polysaccharides(mass concentration 1 g/L) toward 1, 1-diphenyl-2-trinitrophenylhydrazine(DPPH)free radical reached 34.14%.
The ultrahigh pressure extraction technology was used to extract polysaccharides from Polygonatum cyrtonema Hua. The single factor experiment and response surface method were applied to obtain optimal process parameters. Furthermore, the antioxidant activity of Polygonatum polysaccharides was investigated.The results showed that the optimal extraction conditions were as follows: pressure 255 MPa, particle size of raw material 40 mesh, ratio of raw material to extraction agent 1∶17(solids refers the mass of the raw material, g; liquid refers to the volume of the extractant agent, mL), extraction time 9.5 min, extraction temperature room temperature,and extractant agent(water). Under these conditions, the extraction rate of polysaccharides was 25.01%. While the traditional decocting method had an extraction rate of polysaccharides of 19.83% at 100 ℃ for 90 min, which indicated that ultrahigh pressure extraction technology was more effective technology for extracting Polygonatum polysaccharides. The scavenging ability of polysaccharides(mass concentration 1 g/L) toward 1, 1-diphenyl-2-trinitrophenylhydrazine(DPPH)free radical reached 34.14%.
引文
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[20]Zhang Haining(张海宁),Li Xi(李希),Ma Yongkun(马永昆).One possibility on the mechanism of high hydrostatic pressure assisted extraction study on the mechanism of high hydrostatic pressure assisted extraction[J].Food Sicence(食品科学),2017,38(12):190-195.
[21]Yang Xinxin(杨新新),Pan Xiaojuan(潘晓鹃),Yan Hanjing(严寒静),et al.Extraction process of crude polysaccharide from many flower solomonseal rhizome and content determination of polysaccharide in the crude polysaccharide by the method of anthrone-sulfuric acid spectrophotometric[J].Journal of Sichuan of Traditional Chinese Medicine(四川中医),2016,34(1):103-106.
[22]Varela-Santos E,Ochoa-Martinez A,Tabio-Munizaga G,et al.Effect of high hydrostatic pressure(HHP)processing on physicochemical properties,bioactive compounds and shelf-life of pomegranate juice[J].Innovative Food Science and Emerging Technologies,2012,13:13-22.
[23]Casquete R,Castro Sonia M,Ruiz-Moyano S,et al.Evaluation of the effect of high pressure on total phenolic content,antioxidant and antimicrobial activity of citrus peels[J].Innovative Food Science and Emerging Technologies,2015,31:37-44.
[24]Wang Quanze(王全泽),Yuan Tangfeng(袁堂丰),Liu Leilei(刘磊磊),et al.Optimization of flash extraction of total flavonoids from leaves of podocarpus macrophyllus by response surface method and their antioxidant activity[J].Fine Chemicals(精细化工),2018,35(1):65-71.
[25]Wang L B,Liu F C,Wang A X,et al.Purification,characterization and bioactivity determination of a novel polysaccharide from pumpkin(Cucurbita moschata)seeds[J].Food Hydrocolloids,2017,66:357-364.
[26]Liu S W,Xu Q,Li X Y,et al.Effects of high hydrostatic pressure on physicochemical properties,enzymes activity,and antioxidant capacities of anthocyanins extracts of wild Lonicera caerulea berry[J].Innovative Food Science and Emerging Technologies,2016,36:48-58.
[27]Barbosa-Cánovas G V,Hendrickx M,Knorr D.Impact of high pressure processing on total antioxidant activity,phenolic,ascorbic acid,anthocyanin content and colour of strawberry and blackberry purées[J].Innovative Food Science and Emerging Technologies,2009,10(3):308-313.
[28]Zhao S N,Baik O D,Young J C,et al.Pretreatments for the efficient extraction of bioactive compounds from plant-based biomaterials[J].Critical Reviews in Food Science and Nutrition,2014,54(10):1283-1297.
[29]Chan C H,Yusoff R,Ngoh G C,et al.Microwave-assisted extractions of active ingredients from plants[J].Journal of Chromatography A,2011,1218(37):6213-6225.
[30]Xi J.Ultrahigh pressure extraction of bioactive compounds from plants-A review[J].Critical Reviews in Food Science and Nutrition,2015,57(6):1097-1106.
[31]Donald A M,Baker F S,Smith A C,et al.Fracture of plant tissues and walls as visualized by environmental scanning electron microscopy[J].Annals of Botany,2003,92:73-77.
[32]Jin J H,Chen E H,Hyun J H,et al.Optimization of hot water extraction and ultra high pressure extraction for deer antler[J].Food Science and Biotechnology,2015,24(2):507-512.
[33]Kim M Y,Lee S H,Jang G Y,et al.Changes of phenolic-acids and vitamin E profiles on germinated rough rice(Oryza sativa L.)treated by high hydrostatic pressure[J].Food Chemistry,2017,217:106-111.
[34]Altuner E M,Toku?o?lu?.The effect of high hydrostatic pressure processing on the extraction,retention and stability of anthocyanins and flavonols contents of berry fruits and berry juices[J].International Journal of Food Science&Technology,2013,48:1991-1997.
[35]Katopo H,Song Y,Jane J L.Effect and mechanism of ultrahigh hydrostatic pressure on the structure and properties of starches[J].Carbohydrate Polymers,2002,47(3):233-244.
[36]Phunchaisri C,Apichartsrangkoon A.Effects of ultra-high pressure on biochemical and physical modification of lychee(Litchi chinensis Sonn.)[J].Food Chemistry,2005,93(1):57-64.
[2]Jiang Chengxi(姜程曦),Zhang Tiejun(张铁军),Chen Changqing(陈常青),et al.Research progress in molygonati ohizoma and predictive analysis on Q-marker[J].Chinese Traditional and Herbal Drugs(中草药),2017,48(1):1-16.
[3]Chen Hui(陈辉),Feng Shanshan(冯珊珊),Sun Yanjun(孙彦君),et al.Advances in studies on chemical constituents of three medicinal plants from Polygonatum Mill.and their pharmacological activities[J].Chinese Traditional and Herbal Drugs(中草药),2015,46(15):2329-2338.
[4]Wang Y,Qin S,Pen G,et al.Potential ocular protection and dynamic observation of Polygonatum sibiricum polysaccharide against streptozocin-induced diabetic rats'model[J].Experimental Biology and Medicine,2017,242:92-101.
[5]Zhao Hongli(赵宏丽),Xu Yan(许燕),Zhao Hongyan(赵红岩).Effects of polygonatic rhizome on the expression of SREBP-1c and SCD-1 in type 2 diabetic rats[J].Pharmacology and Clinics of Chinese Materia Medica(中药药理与临床),2015,31(1):106-109.
[6]Duan Hua(段华),Wang Baoqi(王保奇),Zhang Yuewen(张跃文).Anti-tumor effects and mechanism of Rhizoma polygonati polysaccharide on H22 tumor bearing mice[J].Traditional Chinese Drug Research&Clinical Pharmacology(中药新药与临床药理),2014,25(1):5-7.
[7]Zhang Zhongying(张忠英),Wang Guoxian(王国贤),Chen Tingting(陈婷婷),et al.Effect of Polygonatum sibiricum polysaccharides on myocardial fibrosis inrats with diabetes[J].Chinese Journal of Public Health(中国公共卫生),2016,32(6):807-810.
[8]Yang J X,Wu S,Huang X L,et al.Hypolipidemic activity and antiatherosclerotic effect of polysaccharide of Polygonatum sibiricum in rabbit model and related cellular mechanisms[J].EvidenceBased Complementary and Alternative Medicine,2015:391065.
[9]Zhang F,Zhang J G,Wang L H,et al.Effects of Polygonatum sibiricum polysaccharide on learning and memory in a scopolamine-induced mouse model of dementia[J].Neural Regeneration Research,2008,1(3):33-36.
[10]Ko J H,Kwon H S,Yoon J M,et al.Effects of Polygonatum sibiricum rhizome ethanol extract in high-fat diet-fed mice[J].Pharmaceutical Biology,2015,50(4):563-570.
[11]Xu Bingbing(徐兵兵),Ni Sui(倪穗).Content determination and extraction process of polysaccharides from wild Polygonatum sibiricum[J].Chinese Wild Plant Resources(中国野生植物资源),2016,35(4):19-22.
[12]Guo Weiyan(郭未艳),Sun Qiuyan(孙秋燕),Xu Xiaohong(徐晓红),et al.The extraction technology of polysaccharide from Polygonatum kingiamum Coll.et Hemsland its optimization[J].Lishizhen Medicine and Materia Medica Research(时珍国医国药),2013,24(6):1391-1393.
[13]Zhang H X,Cao Y Z,Chen L X,et al.A polysaccharide from Polygonatum sibiricum attenuates amyloid-β-induced neurotoxicity in PC12 cells[J].Carbohydrate Polymers,2015,117:879-886.
[14]Zhou Taoying(周桃英),Chen Nianyou(陈年友),Chen Zhongjian(陈中建),et al.Study on extraction of Polygonatum polysaccharide by ultrasonic-microwave synergistic method[J].Jiangsu Agricultural Sciences(江苏农业科学),2013,41(6):231-233.
[15]Luo Wenxian(骆文灿).Ultrasound-assisted extraction of polysaccharides from Polygonatum filipes[J].Fujian Journal of Agricultural Sciences(福建农业学报),2016,31(4):431-436.
[16]Hu Fang(胡芳),Zhou Juanjuan(周娟娟),Chen Aijuan(陈爱娟).The optimum methods of Polygonatum polysaccharide by microwave-assisted extraction[J].Research and Practice on Chinese Medicines(现代中药研究与实践),2015,29(4):52-54.
[17]Yang Shenming(杨申明),Fan Shuguo(范树国),Wen Meiqiong(文美琼),et al.Optimization of microwave-assisted extraction of polysaccharides from Macadamia integrifolia shelland evaluation of their antioxidant activities[J].Food Science(食品科学),2016,37(10):17-22.
[18]Song Siyuan(宋思圆),Su Ping(苏平),Wang Lijuan(王丽娟),et al.Optimization of ultrasonic-assisted extraction by response surface methodology and antioxidant activities of pectic polysaccharide from okra flowers[J].Food Science(食品科学),2017,38(2):283-289.
[19]Jun X.High-pressure processing as emergent technology for the extraction of bioactive ingredients from plant materials[J].Critical Reviews in Food Science and Nutrition,2013,53:837-852.
[20]Zhang Haining(张海宁),Li Xi(李希),Ma Yongkun(马永昆).One possibility on the mechanism of high hydrostatic pressure assisted extraction study on the mechanism of high hydrostatic pressure assisted extraction[J].Food Sicence(食品科学),2017,38(12):190-195.
[21]Yang Xinxin(杨新新),Pan Xiaojuan(潘晓鹃),Yan Hanjing(严寒静),et al.Extraction process of crude polysaccharide from many flower solomonseal rhizome and content determination of polysaccharide in the crude polysaccharide by the method of anthrone-sulfuric acid spectrophotometric[J].Journal of Sichuan of Traditional Chinese Medicine(四川中医),2016,34(1):103-106.
[22]Varela-Santos E,Ochoa-Martinez A,Tabio-Munizaga G,et al.Effect of high hydrostatic pressure(HHP)processing on physicochemical properties,bioactive compounds and shelf-life of pomegranate juice[J].Innovative Food Science and Emerging Technologies,2012,13:13-22.
[23]Casquete R,Castro Sonia M,Ruiz-Moyano S,et al.Evaluation of the effect of high pressure on total phenolic content,antioxidant and antimicrobial activity of citrus peels[J].Innovative Food Science and Emerging Technologies,2015,31:37-44.
[24]Wang Quanze(王全泽),Yuan Tangfeng(袁堂丰),Liu Leilei(刘磊磊),et al.Optimization of flash extraction of total flavonoids from leaves of podocarpus macrophyllus by response surface method and their antioxidant activity[J].Fine Chemicals(精细化工),2018,35(1):65-71.
[25]Wang L B,Liu F C,Wang A X,et al.Purification,characterization and bioactivity determination of a novel polysaccharide from pumpkin(Cucurbita moschata)seeds[J].Food Hydrocolloids,2017,66:357-364.
[26]Liu S W,Xu Q,Li X Y,et al.Effects of high hydrostatic pressure on physicochemical properties,enzymes activity,and antioxidant capacities of anthocyanins extracts of wild Lonicera caerulea berry[J].Innovative Food Science and Emerging Technologies,2016,36:48-58.
[27]Barbosa-Cánovas G V,Hendrickx M,Knorr D.Impact of high pressure processing on total antioxidant activity,phenolic,ascorbic acid,anthocyanin content and colour of strawberry and blackberry purées[J].Innovative Food Science and Emerging Technologies,2009,10(3):308-313.
[28]Zhao S N,Baik O D,Young J C,et al.Pretreatments for the efficient extraction of bioactive compounds from plant-based biomaterials[J].Critical Reviews in Food Science and Nutrition,2014,54(10):1283-1297.
[29]Chan C H,Yusoff R,Ngoh G C,et al.Microwave-assisted extractions of active ingredients from plants[J].Journal of Chromatography A,2011,1218(37):6213-6225.
[30]Xi J.Ultrahigh pressure extraction of bioactive compounds from plants-A review[J].Critical Reviews in Food Science and Nutrition,2015,57(6):1097-1106.
[31]Donald A M,Baker F S,Smith A C,et al.Fracture of plant tissues and walls as visualized by environmental scanning electron microscopy[J].Annals of Botany,2003,92:73-77.
[32]Jin J H,Chen E H,Hyun J H,et al.Optimization of hot water extraction and ultra high pressure extraction for deer antler[J].Food Science and Biotechnology,2015,24(2):507-512.
[33]Kim M Y,Lee S H,Jang G Y,et al.Changes of phenolic-acids and vitamin E profiles on germinated rough rice(Oryza sativa L.)treated by high hydrostatic pressure[J].Food Chemistry,2017,217:106-111.
[34]Altuner E M,Toku?o?lu?.The effect of high hydrostatic pressure processing on the extraction,retention and stability of anthocyanins and flavonols contents of berry fruits and berry juices[J].International Journal of Food Science&Technology,2013,48:1991-1997.
[35]Katopo H,Song Y,Jane J L.Effect and mechanism of ultrahigh hydrostatic pressure on the structure and properties of starches[J].Carbohydrate Polymers,2002,47(3):233-244.
[36]Phunchaisri C,Apichartsrangkoon A.Effects of ultra-high pressure on biochemical and physical modification of lychee(Litchi chinensis Sonn.)[J].Food Chemistry,2005,93(1):57-64.