正造和反季节龙眼花芽分化过程中内源激素水平的变化
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摘要
研究了正造及反季节龙眼树体叶片及顶芽中内源激素IAA、GA、ABA、iPA、ZR在花芽分化过程中的变化。结果表明,花芽分化过程中叶片及顶芽ABA含量最高,IAA次之,ZR、iPA及GA含量极低。氯酸钾处理可导致叶片及顶芽iPA含量迅速升高;叶片GA含量远高于顶芽,而ABA以顶芽中富集量最大;ZR以顶芽含量较高;虽然IAA在叶片和顶芽都有较高水平,但变化幅度不大。综合各激素变化情况认为,花芽分化过程中iPA、GA、ABA含量变化幅度最大,且ABA在花芽分化的发生部位顶芽富集说明ABA在龙眼花芽分化过程中起更重要的作用。
The endogenous hormone levels of IAA, GA, ABA, iPA, ZR and their changes were investigated in leaves and buds of six longan(Dimocarpus longan Lour.) trees for on-season and off-season flowering development after winter shoot mature. The result showed that the hormones contents of different types were different in vegetative organs, presented a decreasing tendency from ABA to IAA and ZR to iPA and GA. Potassium chlorate treatment could cause iPA content rapidly increase in leaves and buds. GA content in leaves were significantly higher than those of the buds, but the largest ABA content was found in the bud. And high levels of ZR were in the buds. Although IAA had a higher level in leaves and buds, the change magnitude was small. The hormone changes had concluded that, iPA, GA, ABA content changed greatly in the flower bud differentiation process, and ABA was enrichment in buds, the parts of flower bud differentiation occurred, indicating that ABA played a more important role in the process of flower bud differentiation of longan.
The endogenous hormone levels of IAA, GA, ABA, iPA, ZR and their changes were investigated in leaves and buds of six longan(Dimocarpus longan Lour.) trees for on-season and off-season flowering development after winter shoot mature. The result showed that the hormones contents of different types were different in vegetative organs, presented a decreasing tendency from ABA to IAA and ZR to iPA and GA. Potassium chlorate treatment could cause iPA content rapidly increase in leaves and buds. GA content in leaves were significantly higher than those of the buds, but the largest ABA content was found in the bud. And high levels of ZR were in the buds. Although IAA had a higher level in leaves and buds, the change magnitude was small. The hormone changes had concluded that, iPA, GA, ABA content changed greatly in the flower bud differentiation process, and ABA was enrichment in buds, the parts of flower bud differentiation occurred, indicating that ABA played a more important role in the process of flower bud differentiation of longan.
引文
[1] BANGERTH F K.Can regulatory mechanism in fruit growth and development be elucidated through the study of endogenous hormone concentrations?[J].Acta Hort,1998,463:77-88.
[2] FRANKOWSKI K,KESY J,WOJCIECHOWSKI W,et al.Light-and IAA-regulated ACC synthase gene(PnACS)from Pharbitis nil and its possible role in IAA-mediated flower inhibition[J].J Plant Physiol,2009,166:192-202.
[3] HOU Z X,HUANG W D.Immunohistochemical localization of IAA and ABP1 in strawberry shoot apexes during floral induction[J].Planta,2005,222(4):678-687.
[4] MEIJóN M,CA?AL M J,VALLEDOR L,et al.Epigenetic and physiological effects of gibberellin inhibitors and chemical pruners on the floral transition of azalea[J].Physiol Plant,2011,141(3):276-288.
[5] YUAN C Q,AHMAD S,CHENG T R,et al.Red to far-red light ratio modulates hormonal and genetic control of axillary bud outgrowth in Chrysanthemum(Dendranthema grandiflorum ‘Jinba’)[J].Int J Mol Sci,2018,19(6):1-21.
[6] JUE D W,SANG X L,LIU L Q,et al.Identification of WRKY gene family from Dimocarpus longan and its expression analysis during flower induction and abiotic stress responses[J].Int J Mol Sci,2018,19(8):1-20.
[7] LI J X,HOU X J,ZHU J,et al.Identification of genes associated with lemon floral transition and flower development during floral inductive water deficits:A hypothetical model[J].Front Plant Sci,2017,8:1013.
[8] XING L B,ZHANG D,LI Y M,et al.Transcription profiles reveal sugar and hormone signaling pathways mediating flower induction in apple(Malus domestica Borkh.)[J].Plant Cell Physiol,2015,56(10):2052-2068.
[9] SUSAWAENGSUP C,RAYANAKORN M,WONGPORNCHAI S,et al.Investigation of plant hormone level changes in shoot tips of longan(Dimocarpus longan Lour.)treated with potassium chlorate by liquid chromatography-electrospray ionization mass spectrometry[J].Talanta,2011,85(2):897-905.
[10] LUCKWILL L C,SILVA J M.The effects of daminozide and gibberellic acid on flower initiation,growth and fruiting of apple cultivar Golden Delicious[J].Journal of horticultural science,1979,54(3):217-223.
[11] KUROHA T,TOKUNAGA H,KOJIMA M,et al.Functional analyses of LONELY GUY cytokinin-activating enzymes reveal the importance of the direct activation pathway in Arabidopsis[J].Plant cell,2009,21(10):3152-3169.
[12] IOIO R D,GALINHA C,FLETCHER A G,et al.A PHABULOSA/cytokinin feedback loop controls root growth in Arabidopsis[J].Curr Biol,2012,22(18):1699-1704.
[13] FATIMA N,ANIS M.Role of growth regulators on in vitro regeneration and histological analysis in Indian ginseng(Withania somnifera L.)Dunal[J].Physiol Mol Biol Plants,2012,18(1):59-67.
[14] HE D,MATHIASON K,FENNELL A.Auxin and cytokinin related gene expression during active shoot growth and latent bud paradormancy in Vitis riparia grapevine[J].J Plant Physiol,2012,169:643-648.
[15] CHEN Y P,CHEN W,LI X L,et al.Knockdown of LjIPT3 influences nodule development in Lotus japonicus[J].Plant Cell Physiol,2014,55(1):183-193.
[16] ZALEJSKI C,ZHANG Z S,QUETTIER A L,et al.Diacylglycerol pyrophosphate is a second messenger of abscisic acid signaling in Arabidopsis thaliana suspension cells[J].The plant journal,2005,42(2):145-152.
[17] TUTEJA N,SOPORY S K.Chemical signaling under abiotic stress environment in plants[J].Plant Signal Behav,2008,3(8):525-536.
[18] ZHANG F Y,LU X,LV Z Y,et al.Overexpression of the Artemisia orthologue of ABA receptor,AaPYL9,enhances ABA sensitivity and improves artemisinin content in Artemisia annua L.[J].PLoS One,2013,8:1-13.
[19] DE MELO FERREIRA W,KERBAUY G B,KRAUS J E,et al.Thidiazuron influences the endogenous levels of cytokinins and IAA during the flowering of isolated shoots of Dendrobium[J].J Plant Physiol,2006,163(11):1126-1134.
[20] CHEN D,DENG Y T,ZHAO J.Distribution and change patterns of free IAA,ABP 1 and PM H+-ATPase during ovary and ovule development of Nicotiana tabacum L.[J].J Plant Physiol,2012,169:127-136.
[21] BARCISZEWSKI J,MASSINO F,CLARK B F C.Kinetin-A multiactive molecule[J].Int J Biol Macromol,2007,40(3):182-192.
[22] 艾军,王英平,李昌禹,等.五味子花芽分化过程中3种内源激素的消长[J].中国中药杂志,2006,31(1):24-26.
[2] FRANKOWSKI K,KESY J,WOJCIECHOWSKI W,et al.Light-and IAA-regulated ACC synthase gene(PnACS)from Pharbitis nil and its possible role in IAA-mediated flower inhibition[J].J Plant Physiol,2009,166:192-202.
[3] HOU Z X,HUANG W D.Immunohistochemical localization of IAA and ABP1 in strawberry shoot apexes during floral induction[J].Planta,2005,222(4):678-687.
[4] MEIJóN M,CA?AL M J,VALLEDOR L,et al.Epigenetic and physiological effects of gibberellin inhibitors and chemical pruners on the floral transition of azalea[J].Physiol Plant,2011,141(3):276-288.
[5] YUAN C Q,AHMAD S,CHENG T R,et al.Red to far-red light ratio modulates hormonal and genetic control of axillary bud outgrowth in Chrysanthemum(Dendranthema grandiflorum ‘Jinba’)[J].Int J Mol Sci,2018,19(6):1-21.
[6] JUE D W,SANG X L,LIU L Q,et al.Identification of WRKY gene family from Dimocarpus longan and its expression analysis during flower induction and abiotic stress responses[J].Int J Mol Sci,2018,19(8):1-20.
[7] LI J X,HOU X J,ZHU J,et al.Identification of genes associated with lemon floral transition and flower development during floral inductive water deficits:A hypothetical model[J].Front Plant Sci,2017,8:1013.
[8] XING L B,ZHANG D,LI Y M,et al.Transcription profiles reveal sugar and hormone signaling pathways mediating flower induction in apple(Malus domestica Borkh.)[J].Plant Cell Physiol,2015,56(10):2052-2068.
[9] SUSAWAENGSUP C,RAYANAKORN M,WONGPORNCHAI S,et al.Investigation of plant hormone level changes in shoot tips of longan(Dimocarpus longan Lour.)treated with potassium chlorate by liquid chromatography-electrospray ionization mass spectrometry[J].Talanta,2011,85(2):897-905.
[10] LUCKWILL L C,SILVA J M.The effects of daminozide and gibberellic acid on flower initiation,growth and fruiting of apple cultivar Golden Delicious[J].Journal of horticultural science,1979,54(3):217-223.
[11] KUROHA T,TOKUNAGA H,KOJIMA M,et al.Functional analyses of LONELY GUY cytokinin-activating enzymes reveal the importance of the direct activation pathway in Arabidopsis[J].Plant cell,2009,21(10):3152-3169.
[12] IOIO R D,GALINHA C,FLETCHER A G,et al.A PHABULOSA/cytokinin feedback loop controls root growth in Arabidopsis[J].Curr Biol,2012,22(18):1699-1704.
[13] FATIMA N,ANIS M.Role of growth regulators on in vitro regeneration and histological analysis in Indian ginseng(Withania somnifera L.)Dunal[J].Physiol Mol Biol Plants,2012,18(1):59-67.
[14] HE D,MATHIASON K,FENNELL A.Auxin and cytokinin related gene expression during active shoot growth and latent bud paradormancy in Vitis riparia grapevine[J].J Plant Physiol,2012,169:643-648.
[15] CHEN Y P,CHEN W,LI X L,et al.Knockdown of LjIPT3 influences nodule development in Lotus japonicus[J].Plant Cell Physiol,2014,55(1):183-193.
[16] ZALEJSKI C,ZHANG Z S,QUETTIER A L,et al.Diacylglycerol pyrophosphate is a second messenger of abscisic acid signaling in Arabidopsis thaliana suspension cells[J].The plant journal,2005,42(2):145-152.
[17] TUTEJA N,SOPORY S K.Chemical signaling under abiotic stress environment in plants[J].Plant Signal Behav,2008,3(8):525-536.
[18] ZHANG F Y,LU X,LV Z Y,et al.Overexpression of the Artemisia orthologue of ABA receptor,AaPYL9,enhances ABA sensitivity and improves artemisinin content in Artemisia annua L.[J].PLoS One,2013,8:1-13.
[19] DE MELO FERREIRA W,KERBAUY G B,KRAUS J E,et al.Thidiazuron influences the endogenous levels of cytokinins and IAA during the flowering of isolated shoots of Dendrobium[J].J Plant Physiol,2006,163(11):1126-1134.
[20] CHEN D,DENG Y T,ZHAO J.Distribution and change patterns of free IAA,ABP 1 and PM H+-ATPase during ovary and ovule development of Nicotiana tabacum L.[J].J Plant Physiol,2012,169:127-136.
[21] BARCISZEWSKI J,MASSINO F,CLARK B F C.Kinetin-A multiactive molecule[J].Int J Biol Macromol,2007,40(3):182-192.
[22] 艾军,王英平,李昌禹,等.五味子花芽分化过程中3种内源激素的消长[J].中国中药杂志,2006,31(1):24-26.