Amphiphilic sodium alginate-vinyl acetate microparticles for drug delivery
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摘要
To overcome the fast or burst release of hydrophilic drugs from hydrophilic alginate-based carriers, hydrophobic molecule(vinyl acetate, VAc) was grafted on alginate(Alg), which was further used to prepare drug carriers. Amphiphilic Alg-g-PVAc hydrogel beads were firstly prepared by emulsification/internal gelation technique for the loading of bovine serum albumin(BSA). Then, chitosan was coated on the surface of beads to form novel amphiphilic Alg-g-PVAc/chitosan(Alg-g-PVAc/CS) microcapsules.The BSA-loading amphiphilic Alg-g-PVAc/chitosan(Alg-g-PVAc/CS) microcapsules display similar morphology and size to the hydrophilic alginate/chitosan(AC) microcapsules. However, the drug loading and loading efficiency of BSA in Alg-g-PVAc/CS microcapsules are higher, and the release rate of BSA from Alg-g-PVAc/CS microcapsules is slower. The results demonstrate that the introduction of hydrophobic PVAc on alginate can effectively help retard the release of BSA, and the higher degree of substitution is,the slower the release rate is. In addition, the complex membrane can also be adjusted to delay the release of BSA. As a whole, amphiphilic sodium alginate-vinyl acetate/CS microparticles could be developed for macromolecular drug delivery.
To overcome the fast or burst release of hydrophilic drugs from hydrophilic alginate-based carriers, hydrophobic molecule(vinyl acetate, VAc) was grafted on alginate(Alg), which was further used to prepare drug carriers. Amphiphilic Alg-g-PVAc hydrogel beads were firstly prepared by emulsification/internal gelation technique for the loading of bovine serum albumin(BSA). Then, chitosan was coated on the surface of beads to form novel amphiphilic Alg-g-PVAc/chitosan(Alg-g-PVAc/CS) microcapsules.The BSA-loading amphiphilic Alg-g-PVAc/chitosan(Alg-g-PVAc/CS) microcapsules display similar morphology and size to the hydrophilic alginate/chitosan(AC) microcapsules. However, the drug loading and loading efficiency of BSA in Alg-g-PVAc/CS microcapsules are higher, and the release rate of BSA from Alg-g-PVAc/CS microcapsules is slower. The results demonstrate that the introduction of hydrophobic PVAc on alginate can effectively help retard the release of BSA, and the higher degree of substitution is,the slower the release rate is. In addition, the complex membrane can also be adjusted to delay the release of BSA. As a whole, amphiphilic sodium alginate-vinyl acetate/CS microparticles could be developed for macromolecular drug delivery.
To overcome the fast or burst release of hydrophilic drugs from hydrophilic alginate-based carriers, hydrophobic molecule(vinyl acetate, VAc) was grafted on alginate(Alg), which was further used to prepare drug carriers. Amphiphilic Alg-g-PVAc hydrogel beads were firstly prepared by emulsification/internal gelation technique for the loading of bovine serum albumin(BSA). Then, chitosan was coated on the surface of beads to form novel amphiphilic Alg-g-PVAc/chitosan(Alg-g-PVAc/CS) microcapsules.The BSA-loading amphiphilic Alg-g-PVAc/chitosan(Alg-g-PVAc/CS) microcapsules display similar morphology and size to the hydrophilic alginate/chitosan(AC) microcapsules. However, the drug loading and loading efficiency of BSA in Alg-g-PVAc/CS microcapsules are higher, and the release rate of BSA from Alg-g-PVAc/CS microcapsules is slower. The results demonstrate that the introduction of hydrophobic PVAc on alginate can effectively help retard the release of BSA, and the higher degree of substitution is,the slower the release rate is. In addition, the complex membrane can also be adjusted to delay the release of BSA. As a whole, amphiphilic sodium alginate-vinyl acetate/CS microparticles could be developed for macromolecular drug delivery.
引文
Boontheekul T, Kong H J, Mooney D J. 2005. Controlling alginate gel degradation utilizing partial oxidation and bimodal molecular weight distribution. Biomaterials,26(15):2 455-2 465, https://doi.org/10.1016/j.biomaterials.2004.06.044.
Ching S H, Bansal N, Bhandari B. 2017. Alginate gel particles—a review of production techniques and physical properties. Critical Reviews in Food Science and Nutrition, 57(6):1 133-1 152, https://doi.org/10.1080/10408398.2014.965773.
Desai S, Perkins J, Harrison B S, Sankar J. 2010. Understanding release kinetics of biopolymer drug delivery microcapsules for biomedical applications. Materials Science and Engineering:B, 168(1-3):127-131, https://doi.org/10.1016/j.mseb.2009.11.006.
Du A W, Stenzel M H. 2014. Drug carriers for the delivery of therapeutic peptides. Biomacromolecules, 15(4):1 097-1114. https://doi.org/10.1021/bm500169p.
Fang Y H, Xiao C M, Wu H, Lin S B. 2005. Graft copolymerization of vinyl acetate onto calcium alginate aquagel. Journal of Huaqiao University(Natural Science),26(2):138-140.(in Chinese with English abstract)
Lee K Y, Mooney D J. 2012. Alginate:properties and biomedical applications. Progress in Polymer Science, 37(1):106-126, https://doi.org/10.1016/j.progpolymsci.2011.06.003.
Liakos I, Rizzello L, Bayer I S, Pompa P P, Cingolani R,Athanassiou A. 2013. Controlled antiseptic release by alginate polymer films and beads. Carbohydrate Polymers, 92(1):176-183. https://doi.org/10.1016/j.carbpol.2012.09.034.
Liu X D, Yu W T, Wang W, Xiong Y, Ma X J, Yuan Q. 2008.Polyelectrolyte microcapsules prepared by alginate and chitosan for biomedical application. Progress in Chemistry, 20(1):126-139.(in Chinese with English abstract)
Lopes M, Abrahim B, Veiga F, Seica R, Cabral L M, Amaud P,Andrade J C, Ribeiro A J. 2017. Preparation methods and applications behind alginate-based particles. Expert Opinion on Drug Delivery, 14(6):769-782, https://doi.org/10.1080/17425247.2016.1214564
Mei L, He F, Zhou R Q, Wu C D, Liang R, Xie R, Ju X J, Wang W, Chu L Y. 2014. Novel intestinal-targeted Ca-alginatebased carrier for pH-responsive protection and release of lactic acid bacteria. ACS Applied Materials&Interfaces,6(8):5 962-5 970, https://doi.org/10.1021/am501011j.
Pawar S N, Edgar K J. 2012. Alginate derivatization:a review of chemistry, properties and applications. Biomaterials,33(11):3279-3305,https://doi.org 10. 1016/j.biomaterials.2012.01.007.
Shin S H, Lee J, Lim K S, Rhim T, Lee S K, Kim Y H, Lee K Y.2013. Sequential delivery of TAT-HSP27 and VEGF usingmicrosphere/hydrogel hybrid systems for therapeutic angiogenesis. Journal of Controlled Release, 166(1):38-45, https://doi.org/10.1016/j.jconrel.2012.12.020.
Song H Y, Yu W T, Gao M, Liu X D, Ma X J. 2013.Microencapsulated probiotics using emulsification technique coupled with internal or external gelation process. Carbohydrate Polymers, 96(1):181-189, https://doi.org/10.1016/j.carbpol.2013.03.068.
Xu G Z, Liu X D, Wang B, Yu W T, Ding D H, Ma X J. 2013.Graft copolymerization of sodium alginate-vinyl acetate and performance evaluation. Chemical Research and Application, 25(8):1 097-1 101.(in Chinese with English abstract)
Xu Y L, Ni C H. 2012. Synthesis of amphiphilic block copolymer via graft polymerization of DAAM and SA.Applied Chemical Industry, 41(2):278-281.(in Chinese with English abstract)
Yang J S, Xie Y J, He W. 2011. Research progress on chemical modification of alginate:a review. Carbohydrate Polymers, 84(1):33-39, https://doi.org/10.1016/j.carbpol.2010.11.048.
Yu W T, Lin J Z, Liu X D, Xie H G, Zhao W, Ma X J. 2010.Quantitative characterization of membrane formation process of alginate-chitosan microcapsules by GPC.Journal of Membrane Science, 346(2):296-301, https://doi.org/10.1016/j.memsci.2009.09.049.
Zhang X L,He H Y,Yen C,Ho W,Lee L J.2008.A biodegradable,immunoprotective, dual nanoporous capsule for cell-based therapies. Biomaterials, 29(31):4 253-4 259, https://doi.org/10.1016/j.biomaterials.2008.07.032.
Zhang Y,Chan H F,Leong K W. 2013. Advanced materials and processing for drug delivery:the past and the future.Advanced Drug Delivery Reviews, 65(1):104-120, https://doi.org/10.1016/j.addr.2012.10.003.
Ching S H, Bansal N, Bhandari B. 2017. Alginate gel particles—a review of production techniques and physical properties. Critical Reviews in Food Science and Nutrition, 57(6):1 133-1 152, https://doi.org/10.1080/10408398.2014.965773.
Desai S, Perkins J, Harrison B S, Sankar J. 2010. Understanding release kinetics of biopolymer drug delivery microcapsules for biomedical applications. Materials Science and Engineering:B, 168(1-3):127-131, https://doi.org/10.1016/j.mseb.2009.11.006.
Du A W, Stenzel M H. 2014. Drug carriers for the delivery of therapeutic peptides. Biomacromolecules, 15(4):1 097-1114. https://doi.org/10.1021/bm500169p.
Fang Y H, Xiao C M, Wu H, Lin S B. 2005. Graft copolymerization of vinyl acetate onto calcium alginate aquagel. Journal of Huaqiao University(Natural Science),26(2):138-140.(in Chinese with English abstract)
Lee K Y, Mooney D J. 2012. Alginate:properties and biomedical applications. Progress in Polymer Science, 37(1):106-126, https://doi.org/10.1016/j.progpolymsci.2011.06.003.
Liakos I, Rizzello L, Bayer I S, Pompa P P, Cingolani R,Athanassiou A. 2013. Controlled antiseptic release by alginate polymer films and beads. Carbohydrate Polymers, 92(1):176-183. https://doi.org/10.1016/j.carbpol.2012.09.034.
Liu X D, Yu W T, Wang W, Xiong Y, Ma X J, Yuan Q. 2008.Polyelectrolyte microcapsules prepared by alginate and chitosan for biomedical application. Progress in Chemistry, 20(1):126-139.(in Chinese with English abstract)
Lopes M, Abrahim B, Veiga F, Seica R, Cabral L M, Amaud P,Andrade J C, Ribeiro A J. 2017. Preparation methods and applications behind alginate-based particles. Expert Opinion on Drug Delivery, 14(6):769-782, https://doi.org/10.1080/17425247.2016.1214564
Mei L, He F, Zhou R Q, Wu C D, Liang R, Xie R, Ju X J, Wang W, Chu L Y. 2014. Novel intestinal-targeted Ca-alginatebased carrier for pH-responsive protection and release of lactic acid bacteria. ACS Applied Materials&Interfaces,6(8):5 962-5 970, https://doi.org/10.1021/am501011j.
Pawar S N, Edgar K J. 2012. Alginate derivatization:a review of chemistry, properties and applications. Biomaterials,33(11):3279-3305,https://doi.org 10. 1016/j.biomaterials.2012.01.007.
Shin S H, Lee J, Lim K S, Rhim T, Lee S K, Kim Y H, Lee K Y.2013. Sequential delivery of TAT-HSP27 and VEGF usingmicrosphere/hydrogel hybrid systems for therapeutic angiogenesis. Journal of Controlled Release, 166(1):38-45, https://doi.org/10.1016/j.jconrel.2012.12.020.
Song H Y, Yu W T, Gao M, Liu X D, Ma X J. 2013.Microencapsulated probiotics using emulsification technique coupled with internal or external gelation process. Carbohydrate Polymers, 96(1):181-189, https://doi.org/10.1016/j.carbpol.2013.03.068.
Xu G Z, Liu X D, Wang B, Yu W T, Ding D H, Ma X J. 2013.Graft copolymerization of sodium alginate-vinyl acetate and performance evaluation. Chemical Research and Application, 25(8):1 097-1 101.(in Chinese with English abstract)
Xu Y L, Ni C H. 2012. Synthesis of amphiphilic block copolymer via graft polymerization of DAAM and SA.Applied Chemical Industry, 41(2):278-281.(in Chinese with English abstract)
Yang J S, Xie Y J, He W. 2011. Research progress on chemical modification of alginate:a review. Carbohydrate Polymers, 84(1):33-39, https://doi.org/10.1016/j.carbpol.2010.11.048.
Yu W T, Lin J Z, Liu X D, Xie H G, Zhao W, Ma X J. 2010.Quantitative characterization of membrane formation process of alginate-chitosan microcapsules by GPC.Journal of Membrane Science, 346(2):296-301, https://doi.org/10.1016/j.memsci.2009.09.049.
Zhang X L,He H Y,Yen C,Ho W,Lee L J.2008.A biodegradable,immunoprotective, dual nanoporous capsule for cell-based therapies. Biomaterials, 29(31):4 253-4 259, https://doi.org/10.1016/j.biomaterials.2008.07.032.
Zhang Y,Chan H F,Leong K W. 2013. Advanced materials and processing for drug delivery:the past and the future.Advanced Drug Delivery Reviews, 65(1):104-120, https://doi.org/10.1016/j.addr.2012.10.003.