金观音与其亲本差异基因表达的遗传分析
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摘要
从金观音—铁观音和金观音—黄旦差减文库中获得差异基因并进行功能分类;采用荧光定量PCR技术检测金观音与其亲本间差异基因表达量的变化,分析差异基因的表达模式,同时检测具有超高亲表达和低于双亲表达模式的基因在其他两个F_1黄观音和金玫瑰上表达量的差异.结果表明:差异基因包含参与生长发育、逆境胁迫和次生代谢等9类功能;与生长发育、逆境胁迫和次生代谢有关的基因在金观音及其亲本中均有表达,依据表达量的差异可分为5种表达模式;根据3个F_1品种与其共同父母本荧光定量表达量的差异,可将F_1品种的表达分为均超过双亲(杂种优势)、低于双亲(杂种劣势)、部分超过双亲和部分低于双亲(兼具杂种优、劣势)3种类型.
To analyze the expression pattern of differential genes between Jinguanyin and its parents, differential genes were firstly obtained and classified from the suppression subtractive library between Jinguanyin-Tieguanyin and Jinguanyin-Huangdan, which was followed by quantification by quantitative real-time PCR analysis. Moreover, expression of genes with ultra-high parent and lower parent expression patterns in other 2 generations of Huangguanyin and Jinmeigui was also quantified and compared. The results showed that the differential genes comprised of 9 functions, including growth and development, adversity and stress, and secondary metabolism. They were all expressed in Jinguanyin and its parents, which can be divided into 5 expression patterns based on expression quantity. According to the differences in quantitative expression among Jinguanyin, Huangguanyin, Jinmeigui and their parents, the screened genes can be divided into 3 types, namely gene with expression level for 3 generations which were all higher than their parents(heterosis), gene with expression level which were all lower than their parents(hybrids weakness), and gene with some expression level which were more than their parents and some less than their parents(both heterosis and hybrids weakness).
To analyze the expression pattern of differential genes between Jinguanyin and its parents, differential genes were firstly obtained and classified from the suppression subtractive library between Jinguanyin-Tieguanyin and Jinguanyin-Huangdan, which was followed by quantification by quantitative real-time PCR analysis. Moreover, expression of genes with ultra-high parent and lower parent expression patterns in other 2 generations of Huangguanyin and Jinmeigui was also quantified and compared. The results showed that the differential genes comprised of 9 functions, including growth and development, adversity and stress, and secondary metabolism. They were all expressed in Jinguanyin and its parents, which can be divided into 5 expression patterns based on expression quantity. According to the differences in quantitative expression among Jinguanyin, Huangguanyin, Jinmeigui and their parents, the screened genes can be divided into 3 types, namely gene with expression level for 3 generations which were all higher than their parents(heterosis), gene with expression level which were all lower than their parents(hybrids weakness), and gene with some expression level which were more than their parents and some less than their parents(both heterosis and hybrids weakness).
引文
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[2] 叶乃兴.乌龙茶种质资源的利用与品种创新[J].福建茶叶,2006,29(3):2-4.
[3] 陈薇宇.福建乌龙茶品种推广应用研究[D].福州:福建农林大学,2016.
[4] 郭吉春,叶乃兴,杨如兴,等.茶树杂交种金观音、黄观音的选育与应用[J].福建茶叶,2008,30(1):11-14.
[5] 张冬桃.乌龙茶做青过程香气形成相关基因的分离与表达[D].福州:福建农林大学,2016.
[6] 俞滢.茉莉花开放过程中香气相关差异基因的克隆与表达分析[D].福州:福建农林大学,2016.
[7] 蔡韡韡.水杨酸诱导下(木奈)抗性相关基因的分离与表达研究[D].福州:福建农林大学,2016.
[8] SUN M L, WANG Y S, YANG D Q, et al. Reference genes for real-time fluorescence quantitative PCR in Camellia sinensis [J]. Chinese Bulletin of Botany, 2010,45(5):579-587.
[9] LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2[-Delta DeltaC(T)] Method [J]. Methods, 2001,25(4):402-408.
[10] 郭吉春,杨如兴,叶乃兴,等.10个茶树高优新品种的育成与应用[J].福建茶叶,2003(3):19-21.
[11] 黄爱萍,邓朝军,陈秀萍,等.龙眼杂交后代果实性状遗传变异研究[J].福建农业学报,2006,21(4):342-345.
[12] 许晨璐,孙晓梅,张守攻.基因差异表达与杂种优势形成机制探讨[J].遗传,2013,35(6):714-726.
[13] LI X H, WEI Y L, NETTLETON D, et al. Comparative gene expression profiles between heterotic and non-heterotic hybrids of tetraploid Medicago sativa [J]. BMC Plant Biol, 2009,9:107-115.
[14] 萧力争,苏晓倩,李勤,等.紫芽品种茶树春梢芽叶生化成分分析[J].福建农林大学学报(自然科学版),2009,38(3):30-33.
[15] 周琼琼,孙威江.茶树芽叶紫化的生理生化分析及其关键酶基因的表达[J].生物技术通报,2015,31(1):86-91.
[16] 陈林,张应根,陈键,等.清香型乌龙茶品质形成过程中香气组成化学模式的动态变化规律[J].茶叶科学,2013(1):53-59.
[17] ZHANG H Y, HE H, CHEN L B, et al. A genomewide transcription analysis reveals a close correlation of promoter INDEL polymorphism and heterotic gene expression in rice hybrids [J]. Mol Plant, 2008,1(5):720-731.
[18] HUANG Z Y, ZHAO B R, L V Q M, et al. Heterosis expression of hybrid rice in natural and short-day length conditions [J]. Chinese Journal of Rice Science, 2015,22(2):81-88.
[19] SHULL G H. The composition of a field of maize [J]. Am Breed Assoe, 1908,4(1):296-301.
[20] 盖钧镒,章元明,王建康.植物数量性状遗传体系[M].北京:科学出版社,2003:145-156.