三种车前草的黄酮提取、纯化及其抗氧化活性分析
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摘要
本研究采用响应面设计优化超声辅助提取车前总黄酮的最佳条件,然后用此条件提取大车前和平车前总黄酮,并探究大孔树脂纯化对三种车前草总黄酮抗氧化活性的影响。结果表明,在超声温度60℃、乙醇浓度70%的条件下,车前总黄酮最佳提取工艺参数为液料比20:1 m L/g、超声时间80 min、超声功率210 W,车前、大车前和平车前的总黄酮得率分别为5.04%、2.86%和1.22%。无论是纯化前还是纯化后,大车前总黄酮的还原力和对羟基自由基的清除作用最强,平车前最弱;车前总黄酮对DPPH自由基的清除作用最大,平车前最弱。纯化前后的还原力和对DPPH自由基、羟基自由基的清除作用都接近Vc的水平。
In this research, the optimal cond itions of ulteasonic-assisted extraction of total flavonoids from P.asiatica were determined by response surface methodology, which was later used to extract flavonoids from P.major and P. depressa and to investigate the effect of macroporous resin purification on the antioxidant activity of the total flavonoids of three species. The results showed that under the ultrasonic temperature of 60℃ and ethanol concentration of 70%, the optimal extraction parameters of total flavonoids from P. asiatica were liquid-solid ratio of 20:1 m L/g, ultrasonic time of 80 min, and ultrasonic power of 210 W. The yield of total flavonoids of P.asiatica, P. major and P. depressa were 5.04%, 2.86% and 1.22%, respectively. Both crude and purified flavonoids from P. major showed the strongest hydroxyl radical scavenging activity and reducing power, while P. depressa showed the weakest power. The flavonoids from P. asiatica displayed the strongest DPPH radical scavenging activity, while P. depressa was the weakest. The reducing power, DPPH radical and hydroxyl radical scavenging before and after purification, were all closed to the Vc level.
In this research, the optimal cond itions of ulteasonic-assisted extraction of total flavonoids from P.asiatica were determined by response surface methodology, which was later used to extract flavonoids from P.major and P. depressa and to investigate the effect of macroporous resin purification on the antioxidant activity of the total flavonoids of three species. The results showed that under the ultrasonic temperature of 60℃ and ethanol concentration of 70%, the optimal extraction parameters of total flavonoids from P. asiatica were liquid-solid ratio of 20:1 m L/g, ultrasonic time of 80 min, and ultrasonic power of 210 W. The yield of total flavonoids of P.asiatica, P. major and P. depressa were 5.04%, 2.86% and 1.22%, respectively. Both crude and purified flavonoids from P. major showed the strongest hydroxyl radical scavenging activity and reducing power, while P. depressa showed the weakest power. The flavonoids from P. asiatica displayed the strongest DPPH radical scavenging activity, while P. depressa was the weakest. The reducing power, DPPH radical and hydroxyl radical scavenging before and after purification, were all closed to the Vc level.
引文
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Liu F.,and Ng T.B.,2000,Antioxidative and free radical scavenging activities of selected medicinal herbs,Life Sciences,66(8):725-735
Naik G.H.,Priyadarsini K.I.,Satav J.G.,Banavalikar M.M.,Sohoni D.P.,Biyani M.K.,and Mohan H.,2003,Comparative antioxidant activity of individual herbal components used in Ayurvedic medicine,Phytochemistry,63(1):97-104
Razali N.,Razab R.,Junit S.M.,and Aziz A.A.,2008,Radica scavenging and reducing properties of extracts of cashew shoots(Anacardium occidentale),Food Chemistry,111(1)38-44
Tian Y.J.,Zhu L.,Chen J.,and Lin F.L.,2011,Flavonoids ultrasonic extraction from fruit Canarium album(Lour.)and its antioxidant activity,Shiping Yanjiu Yu Kaifa(Food Research and Development),32(1):17-22(田应娟,朱良,陈健,林福兰,2011,超声强化提取橄榄黄酮类物质及其抗氧化活性研究,食品研究与开发,32(1):17-22)
Xia D.Z.,Liu J.E.,and Chen P.P.,2009,The scavenging activities of total flavonoids from Plantago asiatica linn on free radicals and its protective effects on oxidative damage in mice,Keji Tongbao(Bulletin of Science and Technology),25(6):792-797(夏道宗,刘杰尔,陈佩佩,2009,车前草总黄酮清除自由基及对小鼠氧化损伤的保护作用,科技通报,25(6):792-797)
Yu J.Y.,and Wang C.Y.,2016,Biologically active substances in extract of Plantago major L.and Cyamopsis tetragonoloba L.,Siliao Ziyuan(Feed Resources),1:45-47(于继英,王春雨,2016,车前草和瓜尔豆提取物中的生物活性物质,饲料资源,1:45-47)
Zhang X.Q.,Qu W.,and Liang J.Y.,2013,Research progresses on chemical constituents and pharmacological activities of Plantago spp,Haixia Yaoxue(Strait Pharmaceutical Journal),25(11):1-8(张雪芹,曲玮,梁敬钰,2013,车前草化学成分和药理作用研究进展,海峡药学,25(11):1-8)
Li M.J.,Liu L.Fu C.Y.,and Li R.D.,2013,Analysis of flavonoid compounds in plantain by high performance liquid chromatography,Guangpu Shiyanshi(Chinese Journal of Spectroscopy Laboratory),30(3):1437-1443(李明娟,刘磊,傅春燕,李荣东,2013,高效液相色谱法分析车前草中的黄酮化合物,光谱实验室,30(3):1437-1443)
Li X.M.,Li X.L.,and Zhou A.G.,2007,Evaluation of antioxidant activity of the polysaccharides extracted from lycium barbarum fruits in vitro,European Polymer Journal,43(2):488-497
Liu F.,and Ng T.B.,2000,Antioxidative and free radical scavenging activities of selected medicinal herbs,Life Sciences,66(8):725-735
Naik G.H.,Priyadarsini K.I.,Satav J.G.,Banavalikar M.M.,Sohoni D.P.,Biyani M.K.,and Mohan H.,2003,Comparative antioxidant activity of individual herbal components used in Ayurvedic medicine,Phytochemistry,63(1):97-104
Razali N.,Razab R.,Junit S.M.,and Aziz A.A.,2008,Radica scavenging and reducing properties of extracts of cashew shoots(Anacardium occidentale),Food Chemistry,111(1)38-44
Tian Y.J.,Zhu L.,Chen J.,and Lin F.L.,2011,Flavonoids ultrasonic extraction from fruit Canarium album(Lour.)and its antioxidant activity,Shiping Yanjiu Yu Kaifa(Food Research and Development),32(1):17-22(田应娟,朱良,陈健,林福兰,2011,超声强化提取橄榄黄酮类物质及其抗氧化活性研究,食品研究与开发,32(1):17-22)
Xia D.Z.,Liu J.E.,and Chen P.P.,2009,The scavenging activities of total flavonoids from Plantago asiatica linn on free radicals and its protective effects on oxidative damage in mice,Keji Tongbao(Bulletin of Science and Technology),25(6):792-797(夏道宗,刘杰尔,陈佩佩,2009,车前草总黄酮清除自由基及对小鼠氧化损伤的保护作用,科技通报,25(6):792-797)
Yu J.Y.,and Wang C.Y.,2016,Biologically active substances in extract of Plantago major L.and Cyamopsis tetragonoloba L.,Siliao Ziyuan(Feed Resources),1:45-47(于继英,王春雨,2016,车前草和瓜尔豆提取物中的生物活性物质,饲料资源,1:45-47)
Zhang X.Q.,Qu W.,and Liang J.Y.,2013,Research progresses on chemical constituents and pharmacological activities of Plantago spp,Haixia Yaoxue(Strait Pharmaceutical Journal),25(11):1-8(张雪芹,曲玮,梁敬钰,2013,车前草化学成分和药理作用研究进展,海峡药学,25(11):1-8)