丹蛭降糖胶囊对糖尿病动脉硬化模型大鼠胸主动脉p22phox mRNA、p47phox mRNA表达的影响
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摘要
目的:探讨益气养阴活血方丹蛭降糖胶囊(DJC)对糖尿病大血管病变的作用机制。方法:SD雄性大鼠60只,适应性喂养1周,随机分为空白对照组10只与造模组50只。空白对照组予普通饲料喂养。造模组按70万IU/kg的总剂量灌胃维生素D3,分3天给完,同时高脂饲料喂养,诱发快速动脉硬化的大鼠模型,4周以后,采用35 mg/kg链脲佐菌素(STZ)腹腔注射,诱发糖尿病合并动脉硬化大鼠模型,最终造模成功的大鼠随机分为模型组,DJC低、中、高剂量组,分别予以相应药物灌胃,疗程12周。检测治疗前后大鼠空腹血糖(FBG)、胸主动脉p22phox mRNA及p47phox mRNA的表达。结果:与空白对照组比较,模型组大鼠治疗前后FBG水平显著升高,胸主动脉p22phox mRNA、p47phox mRNA表达水平升高,差异均有统计学意义(P<0.01)。与治疗前比较,DJC中、高剂量组大鼠FBG降低,差异有统计学意义(P<0.01)。与模型组比较,DJC中、高剂量组大鼠FBG水平降低,胸主动脉p22phox mRNA、p47phox mRNA表达降低,差异均有统计学意义(P<0.01)。与DJC低剂量组比较,DJC中、高剂量组大鼠FBG水平较低,胸主动脉p22phox mRNA、p47phox mRNA表达较低,差异均有统计学意义(P<0.05)。结论:丹蛭降糖胶囊能够降低糖尿病大鼠FBG,降低胸主动脉p22phox mRNA和p47phox mRNA的表达,抑制氧化应激,从而有效防治和延缓糖尿病大血管病变。
Objective: To explore the mechanism of Yiqi Yangyin Huoxue prescription and Danzhi Jiangtang capsules(DJC) on diabetic macroangiopathy. Methods:After conducting adaptive feeding to 60 cases of male SD rats for one week,divided them into the blank control group and the modeling group randomly,10 cases and 50 cases in each group respectively. The blank control group was fed with normal diet. The modeling group was given vitamin D3 with a total dose of 700,000 IU/kg by gavage in 3 days and was fed with high fat diet meanwhile to induce and establish rat models with rapid arteriosclerosis. After 4 weeks,35 mg/kg STZ intraperitoneal injection was used to induce and establish rat models with diabetes and arteriosclerosis. Those rats with successful modeling were randomly divided into the model group,the DJC groups of low,middle and high dose,and were given corresponding drugs by gavage respectively for a course of 12 weeks. Detected the expression of fasting blood glucose(FBG) and p22 phox m RNA and p47 phox m RNA of thoracic aorta in rats before and after treatment. Results: Compared with that in the blank control group, the level of FBG in the model group was increased more significantly before and after treatment,and expression levels of p22 phox m RNA and p47 phox m RNA of thoracic aorta were increased,differences being significant(P < 0.01). Levels of FBG in the DJC groups of middle and high dose were decreased when compared with those before treatment, the difference being significant(P < 0.01). Compared with those in the model group,the levels of FBG and expression levels of p22 phox m RNA and p47 phox m RNA of thoracic aorta in the DJC groups of middle and high dose were decreased,differences being significant(P < 0.01). Compared with those in the DJC group of low dose,levels of FBG and expression levels of p22 phox m RNA and p47 phox m RNA of thoracic aorta in the DJC groups of middle and high dose were lower, differences being significant(P < 0.05). Conclusion: Danzhi Jiangtang capsules can reduce fasting plasma glucose of rats with diabetes, reduce the expression of p22 phox m RNA and p47 phox m RNA of thoracic aorta, and inhibit oxidative stress, so as to effectively prevent,treat and delay diabetic macroangiopathy.
Objective: To explore the mechanism of Yiqi Yangyin Huoxue prescription and Danzhi Jiangtang capsules(DJC) on diabetic macroangiopathy. Methods:After conducting adaptive feeding to 60 cases of male SD rats for one week,divided them into the blank control group and the modeling group randomly,10 cases and 50 cases in each group respectively. The blank control group was fed with normal diet. The modeling group was given vitamin D3 with a total dose of 700,000 IU/kg by gavage in 3 days and was fed with high fat diet meanwhile to induce and establish rat models with rapid arteriosclerosis. After 4 weeks,35 mg/kg STZ intraperitoneal injection was used to induce and establish rat models with diabetes and arteriosclerosis. Those rats with successful modeling were randomly divided into the model group,the DJC groups of low,middle and high dose,and were given corresponding drugs by gavage respectively for a course of 12 weeks. Detected the expression of fasting blood glucose(FBG) and p22 phox m RNA and p47 phox m RNA of thoracic aorta in rats before and after treatment. Results: Compared with that in the blank control group, the level of FBG in the model group was increased more significantly before and after treatment,and expression levels of p22 phox m RNA and p47 phox m RNA of thoracic aorta were increased,differences being significant(P < 0.01). Levels of FBG in the DJC groups of middle and high dose were decreased when compared with those before treatment, the difference being significant(P < 0.01). Compared with those in the model group,the levels of FBG and expression levels of p22 phox m RNA and p47 phox m RNA of thoracic aorta in the DJC groups of middle and high dose were decreased,differences being significant(P < 0.01). Compared with those in the DJC group of low dose,levels of FBG and expression levels of p22 phox m RNA and p47 phox m RNA of thoracic aorta in the DJC groups of middle and high dose were lower, differences being significant(P < 0.05). Conclusion: Danzhi Jiangtang capsules can reduce fasting plasma glucose of rats with diabetes, reduce the expression of p22 phox m RNA and p47 phox m RNA of thoracic aorta, and inhibit oxidative stress, so as to effectively prevent,treat and delay diabetic macroangiopathy.
引文
[1]陈明卫,夏同佳,方朝晖,等.丹蛭降糖胶囊改善高脂饮食诱导的肥胖大鼠骨骼肌胰岛素抵抗机制的初步研究[J].中华临床医师杂志:电子版,2014,8(9):1697-1702.
[2]方朝晖,赵进东,鲍陶陶,等.丹蛭降糖胶囊对2型糖尿病患者FINS和IRI作用有效性的系统评价[J].世界中医药,2014,9(12):1679-1681.
[3]刘珊珊,李中南,许成群,等.丹蛭降糖胶囊对糖尿病大鼠血清TNF-α和CXCL-5的影响[J].安徽中医药大学学报,2014,33(6):58-61.
[4]Zhao Huifang,Yan Liu,Jian Liu,et al.Effect of Danzhijiangtang capsule on monocyte chemoattract protein-1 m RNA expression in newly diagnosed diabetes subclinical vascular lesions[J].World Gastroenterol,2013,19(19):2963-29683.
[5]江婷,方朝晖,郭呈林,等.丹蛭降糖胶囊联合生活方式干预2型糖尿病患者颈动脉斑块的临床观察[J].成都中医药大学学报,2014,37(1):56-59.
[6]吴吉萍,方朝晖,倪英群.益气养阴活血中药对2型糖尿病合并血管病变的干预研究[J].安徽医药,2014,18(4):735-737.
[7]温进坤,韩梅,杜玮南,等.一种快速建立大鼠动脉粥样硬化模型的实验方法[J].中国老年学杂志,2001,21(1):50-52.
[8]魏伟.药理实验方法学[M].4版.北京:人民卫生出版社,2010:212.
[9]刘毅,李志梁,江腾春,等.黄芪多糖对2型糖尿病患者内皮功能的影响[J].中国当代医药,2015,22(3):110-112.
[10]王亚双,吕肖锋,张敏郁,等.血糖波动对2型糖尿病下肢血管病变患者血管内皮功能的影响及危险因素分析[J].解放军医药杂志,2014,26(8):21-24,28.
[11]林雪松,汪乐,乔亮.高糖及晚期糖基化终末产物环境对血管内皮细胞血管样结构形成的影响[J].上海交通大学学报:医学版,2014,34(5):596-602.
[12]郭敏,王玉晶,邱文丽,等.痛风患者血管内皮功能及颈动脉粥样硬化的临床研究[J].现代生物医学进展,2015,15(3):499-502.
[13]胡海燕,李跃艳,李春霞,等.ATP敏感钾通道在丹参酮ⅡA对抗高糖诱导血管内皮功能损伤中的作用[J].中国老年学杂志,2015,35(1):174-176.
[14]任蓓,潘澄,林鹏,等.高糖诱发氧化应激导致血管内皮细胞凋亡机制研究[J].海峡药学,2013,25(6):268-271.
[15]毛达勇,吕军,周有利,等.2型糖尿病并血管病变患者血浆GSH-PX、SOD、NO、NOS含量的变化[J].微循环学杂志,2005,15(2):59-60.
[2]方朝晖,赵进东,鲍陶陶,等.丹蛭降糖胶囊对2型糖尿病患者FINS和IRI作用有效性的系统评价[J].世界中医药,2014,9(12):1679-1681.
[3]刘珊珊,李中南,许成群,等.丹蛭降糖胶囊对糖尿病大鼠血清TNF-α和CXCL-5的影响[J].安徽中医药大学学报,2014,33(6):58-61.
[4]Zhao Huifang,Yan Liu,Jian Liu,et al.Effect of Danzhijiangtang capsule on monocyte chemoattract protein-1 m RNA expression in newly diagnosed diabetes subclinical vascular lesions[J].World Gastroenterol,2013,19(19):2963-29683.
[5]江婷,方朝晖,郭呈林,等.丹蛭降糖胶囊联合生活方式干预2型糖尿病患者颈动脉斑块的临床观察[J].成都中医药大学学报,2014,37(1):56-59.
[6]吴吉萍,方朝晖,倪英群.益气养阴活血中药对2型糖尿病合并血管病变的干预研究[J].安徽医药,2014,18(4):735-737.
[7]温进坤,韩梅,杜玮南,等.一种快速建立大鼠动脉粥样硬化模型的实验方法[J].中国老年学杂志,2001,21(1):50-52.
[8]魏伟.药理实验方法学[M].4版.北京:人民卫生出版社,2010:212.
[9]刘毅,李志梁,江腾春,等.黄芪多糖对2型糖尿病患者内皮功能的影响[J].中国当代医药,2015,22(3):110-112.
[10]王亚双,吕肖锋,张敏郁,等.血糖波动对2型糖尿病下肢血管病变患者血管内皮功能的影响及危险因素分析[J].解放军医药杂志,2014,26(8):21-24,28.
[11]林雪松,汪乐,乔亮.高糖及晚期糖基化终末产物环境对血管内皮细胞血管样结构形成的影响[J].上海交通大学学报:医学版,2014,34(5):596-602.
[12]郭敏,王玉晶,邱文丽,等.痛风患者血管内皮功能及颈动脉粥样硬化的临床研究[J].现代生物医学进展,2015,15(3):499-502.
[13]胡海燕,李跃艳,李春霞,等.ATP敏感钾通道在丹参酮ⅡA对抗高糖诱导血管内皮功能损伤中的作用[J].中国老年学杂志,2015,35(1):174-176.
[14]任蓓,潘澄,林鹏,等.高糖诱发氧化应激导致血管内皮细胞凋亡机制研究[J].海峡药学,2013,25(6):268-271.
[15]毛达勇,吕军,周有利,等.2型糖尿病并血管病变患者血浆GSH-PX、SOD、NO、NOS含量的变化[J].微循环学杂志,2005,15(2):59-60.