IL-10、AECA在自身免疫肺气肿大鼠中的作用研究
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摘要
目的研究白细胞介素-10(IL-10)、抗血管内皮细胞抗体(AECA)在自身免疫肺气肿大鼠中的作用。方法将人脐静脉内皮细胞向大鼠腹腔内注射建立自身免疫肺气肿模型,并设对照组,21 d建模成功,对右肺组织行HE染色病理学观察,测量肺平均内衬间隔(MLI)及平均肺泡数(MAN),对左肺灌洗并收集灌洗液(BALF),用ELISA检测BALF中的IL-10、AECA。结果与对照组比较,模型组中的MLI升高,MAN降低(P<0.05);与对照组比较,BALF中的AECA升高,IL-10降低(P<0.05)。对模型组大鼠BALF中AECA与IL-10行相关性分析,发现AECA与IL-10呈负相关(r=-0.64,P<0.05)。结论 IL-10的下调与AECA的增加共同促进了大鼠自身免疫肺气肿的形成,推测可能机制与AECA抑制IL-10的表达有关。
Objective To study the expression of interleukin-10(IL-10) in the autoimmune emphysema in rats. Methods The model was established by human umbilical vein endothelial cells, and set up a contral groud,which was successfully modeled in 21 days.The lung tissue sections of rats were observed the morphological changes, and measured the mean linear intercept(MLI) and mean alveolar number(MAN).The left lung was lavaged to prepare BALF.The levels of IL-10 and AECA in BALF were detected by ELISA. Results Compared with the control,the MLI of the model group increased and the MAN decreased, the levels of IL-10 in BALF was lower, however, the AECA in BALF was higher. All the differences were significant(P<0.05). Correlation analysis of AECA and IL-10 in BALF of rats in the model group showed a negative correlation(r=-0.64, P<0.05). Conclusion The decrease in IL-10 and the increase of AECA concentration may have a facilitation effect in autoimmune emphysema,which may be related to the inhibition of the expression of IL-10 by AECA.
Objective To study the expression of interleukin-10(IL-10) in the autoimmune emphysema in rats. Methods The model was established by human umbilical vein endothelial cells, and set up a contral groud,which was successfully modeled in 21 days.The lung tissue sections of rats were observed the morphological changes, and measured the mean linear intercept(MLI) and mean alveolar number(MAN).The left lung was lavaged to prepare BALF.The levels of IL-10 and AECA in BALF were detected by ELISA. Results Compared with the control,the MLI of the model group increased and the MAN decreased, the levels of IL-10 in BALF was lower, however, the AECA in BALF was higher. All the differences were significant(P<0.05). Correlation analysis of AECA and IL-10 in BALF of rats in the model group showed a negative correlation(r=-0.64, P<0.05). Conclusion The decrease in IL-10 and the increase of AECA concentration may have a facilitation effect in autoimmune emphysema,which may be related to the inhibition of the expression of IL-10 by AECA.
引文
[1] Taraseviciene-Stewart L,Scerbavicius R,Choe KH,et al.An animal model of autoimmune emphysema[J].Am J Respir Crit Care Med,2005,171(7):734-742.
[2] 张程,路苹,晏木云,等.肺泡灌洗液TNF-α、IL-6、和MMP-9在大鼠自身免疫性肺气肿发病中的作用[J].广东医学,2013,7(34)1006-1007.
[3] 汪新龙,刘朝晖,梁志科,等.慢性阻塞性肺疾病大鼠气道炎症与白细胞介素-10的相关性研究[J].中国呼吸与危重监护杂志,2014,13(2):142-145.
[4] 路苹.穿孔素基因在自身免疫性肺气肿大鼠脾脏CD4+T淋巴细胞中的表达[D].遵义医学院,2012.
[5] 李莉,王向东.大鼠被动吸烟气道磷酸二酯酶4D和白细胞介素-8的关系研究[J].临床肺科杂志,2010,15(11):1570-1573.
[6] Brusselle GG,Demoor T,Bracke KR,et al.Lymphoid follicles in(very)severe COPD:beneficial or harmful?[J].European Respiratory Journal,2009,34(1):219-230.
[7] Zhang C,Yan MY,Lu P,et al.Hypomethylation of perforin regulatory elements in CD4+ T cells from rat spleens contributes to the development of autoimmune emphysema[J].Respirology,2014,19(3):376-381.
[8] Legendre P,Régent,Alexis,et al.Anti-endothelial cell antibodies in vasculitis:a systematic review[J].Autoimmunity Reviews,2017,16(2):146-153.
[9] 于阅尽.抗内皮细胞抗体与相关疾病临床研究进展[J].医学综述,2012,18(4):494-497.
[10] Karayama M,Inui N,Suda T,et al.Antiendothelial cell antibodies in patients with COPD[J].Chest,2010,138(6):1303-1308.
[11] 马丽,张璐,张程,等.抗血管内皮细胞抗体在吸烟肺气肿大鼠肺泡隔细胞凋亡中的作用及甲泼尼龙的干预效果[J].广东医学,2016,37(10):1447-1450.
[12] Lee JH,Hanaoka M,Kitaguchi Y,et al.Imbalance of apoptosis and cell proliferation contributes to the development and persistence of emphysema[J].Lung,2012,190(1):69-82.
[13] Zhang XY,Zhang C,Sun QY,et al.Infliximab protects against pulmonary emphysema in smoking rats[J].中华医学杂志(英文版),2011,124(16):2502-2506.
[14] Demedts IK,Demoor T,Bracke KR,et al.Role of apoptosis in the pathogenesis of COPD and pulmonary emphysema[J].Respir Res,2006,7(1):53.
[15] 杨晓东,胡大鹏,鲍文华,等.慢性阻塞性肺疾病患者血清白细胞介素-9、-10的水平及意义[J].中国老年学杂志,2016,(36):3998-3999.
[16] 高延秋,张华,陈建丽,等.血必净注射液对重症肺炎患者血清中白细胞介素-6、白细胞介素-10和肿瘤坏死因子-α影响的研究[J].中国医院用药评价与分析,2014(10):881-883.
[17] Penaloza HF,Nieto PA,Munozdurango N,et al.Interleukin-10 plays a key role in the modulation of neutrophils recruitment and lung inflammation during infection by streptococcus pneumoniae[J].Immunology,2015,146(1):100-112.
[18] Xystrakis E,Kusumakar S,Boswell S,et al.Reversing the defective induction of IL-10 secreting rgulabry T cells in glucocortieoid-resistant asthma a patients[J].Clin Invest,2006,116:146-155.
[19] 吉瑜虹,黄伸伸,巩秀丽,等.人参皂苷预处理对急性肺损伤大鼠肿瘤坏死因子-α和白细胞介素-6及白细胞介素-10水平的影响[J].中国临床药理学杂志,2017,33(16):1552-1555.
[20] 李雪莲,凌杰.慢性阻塞性肺疾病患者治疗前后血浆炎症因子的变化[J].中国药物与临床,2012,12(4):478-479.
[21] 李珍宇,蒲荣,梁晶晶,等.血清 PCT、IL-6与IL-10对社区获得性肺炎患者诊断差异性研究[J].医学检验,2011,8(17):87-88.
[2] 张程,路苹,晏木云,等.肺泡灌洗液TNF-α、IL-6、和MMP-9在大鼠自身免疫性肺气肿发病中的作用[J].广东医学,2013,7(34)1006-1007.
[3] 汪新龙,刘朝晖,梁志科,等.慢性阻塞性肺疾病大鼠气道炎症与白细胞介素-10的相关性研究[J].中国呼吸与危重监护杂志,2014,13(2):142-145.
[4] 路苹.穿孔素基因在自身免疫性肺气肿大鼠脾脏CD4+T淋巴细胞中的表达[D].遵义医学院,2012.
[5] 李莉,王向东.大鼠被动吸烟气道磷酸二酯酶4D和白细胞介素-8的关系研究[J].临床肺科杂志,2010,15(11):1570-1573.
[6] Brusselle GG,Demoor T,Bracke KR,et al.Lymphoid follicles in(very)severe COPD:beneficial or harmful?[J].European Respiratory Journal,2009,34(1):219-230.
[7] Zhang C,Yan MY,Lu P,et al.Hypomethylation of perforin regulatory elements in CD4+ T cells from rat spleens contributes to the development of autoimmune emphysema[J].Respirology,2014,19(3):376-381.
[8] Legendre P,Régent,Alexis,et al.Anti-endothelial cell antibodies in vasculitis:a systematic review[J].Autoimmunity Reviews,2017,16(2):146-153.
[9] 于阅尽.抗内皮细胞抗体与相关疾病临床研究进展[J].医学综述,2012,18(4):494-497.
[10] Karayama M,Inui N,Suda T,et al.Antiendothelial cell antibodies in patients with COPD[J].Chest,2010,138(6):1303-1308.
[11] 马丽,张璐,张程,等.抗血管内皮细胞抗体在吸烟肺气肿大鼠肺泡隔细胞凋亡中的作用及甲泼尼龙的干预效果[J].广东医学,2016,37(10):1447-1450.
[12] Lee JH,Hanaoka M,Kitaguchi Y,et al.Imbalance of apoptosis and cell proliferation contributes to the development and persistence of emphysema[J].Lung,2012,190(1):69-82.
[13] Zhang XY,Zhang C,Sun QY,et al.Infliximab protects against pulmonary emphysema in smoking rats[J].中华医学杂志(英文版),2011,124(16):2502-2506.
[14] Demedts IK,Demoor T,Bracke KR,et al.Role of apoptosis in the pathogenesis of COPD and pulmonary emphysema[J].Respir Res,2006,7(1):53.
[15] 杨晓东,胡大鹏,鲍文华,等.慢性阻塞性肺疾病患者血清白细胞介素-9、-10的水平及意义[J].中国老年学杂志,2016,(36):3998-3999.
[16] 高延秋,张华,陈建丽,等.血必净注射液对重症肺炎患者血清中白细胞介素-6、白细胞介素-10和肿瘤坏死因子-α影响的研究[J].中国医院用药评价与分析,2014(10):881-883.
[17] Penaloza HF,Nieto PA,Munozdurango N,et al.Interleukin-10 plays a key role in the modulation of neutrophils recruitment and lung inflammation during infection by streptococcus pneumoniae[J].Immunology,2015,146(1):100-112.
[18] Xystrakis E,Kusumakar S,Boswell S,et al.Reversing the defective induction of IL-10 secreting rgulabry T cells in glucocortieoid-resistant asthma a patients[J].Clin Invest,2006,116:146-155.
[19] 吉瑜虹,黄伸伸,巩秀丽,等.人参皂苷预处理对急性肺损伤大鼠肿瘤坏死因子-α和白细胞介素-6及白细胞介素-10水平的影响[J].中国临床药理学杂志,2017,33(16):1552-1555.
[20] 李雪莲,凌杰.慢性阻塞性肺疾病患者治疗前后血浆炎症因子的变化[J].中国药物与临床,2012,12(4):478-479.
[21] 李珍宇,蒲荣,梁晶晶,等.血清 PCT、IL-6与IL-10对社区获得性肺炎患者诊断差异性研究[J].医学检验,2011,8(17):87-88.