摘要
目的:检测Pik3cb在小鼠下颌第一磨牙牙胚发育不同阶段的表达分布及变化规律,进一步揭示Pik3cb对牙形态发生的影响。方法:制备原位杂交探针,及小鼠牙胚发育标志时间点的牙胚冰冻切片,通过原位杂交方法进一步显示Pik3cb在小鼠下颌第一磨牙牙胚发育不同时期的表达情况。结果:蕾状期Pik3cb明显表达于牙板上皮,帽状期开始大量表达于釉上皮与牙乳头,进入钟状期表达部位趋于集中,尤以内釉上皮及后续新形成硬组织周围的高柱状成釉细胞处最为明显。结论:Pik3cb在牙源性上皮及成釉细胞表达,并可能通过PI3K/PTEN/AKT/mTOR信号通路与成釉细胞瘤行为相关。
Objective:To detect the expression pattern and variation of Pik3 cb at different developmental stages of mouse mandibular first molar.Methods:The probe for in situ hybridization was prepared.Mouse embryos at different times were obtained to get cryostat serial sections of tooth germ for ISH.Results:In bud period,Pik3 cb obviously expressed in dental lamina epithelium.In bonnet period,the Pik3 cb began to express in the enamel epithelium and dental papilla.In bell stage,the expression of Pik3 cb was especially within the enamel epithelium and subsequently in the new formation of hard tissues surrounding the columnar cells into glaze.Conclusion:Pik3cb expresses in dental epithelial cells and ameloblasts and it is likely to associate with ameloblastoma through PI3K/PTEN/AKT/mTOR signaling pathway.
引文
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