猕猴桃根多糖对角叉菜胶致小鼠尾部血栓形成的影响
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摘要
目的探讨猕猴桃根多糖对角叉菜胶致小鼠尾部血栓形成的影响。方法将60只小鼠分为对照组、模型组、阿司匹林组、猕猴桃根多糖低剂量组[40 mg/(kg·d)]、中剂量组[80 mg/(kg·d)]、高剂量组[160 mg/(kg·d)],每组10只,除对照组和模型组外,各组小鼠分别给予相应药物灌胃10 d。除对照组外,其余各组小鼠最后1次灌胃1 h后均给予腹腔注射角叉菜胶构建小鼠尾部血栓模型。24 h后计算各组小鼠相对黑尾长度(RATL)及RATL抑制率,检测凝血功能,并取小鼠尾部组织观察尾部血栓形成情况。结果模型组小鼠血栓发生率为100%,而对照组和多糖高剂量组小鼠未发生黑尾。猕猴桃根多糖中剂量组小鼠的RATL短于模型组(P<0.05),各剂量多糖组小鼠的RATL抑制率均高于阿司匹林组。多糖中高剂量组小鼠的凝血酶原时间、活化部分凝血活酶时间均长于模型组、阿司匹林组(均P<0.05)。病理结果显示,模型组、阿司匹林组、猕猴桃根多糖低、中剂量组小鼠尾静脉均有不同程度的血栓形成,而猕猴桃根多糖高剂量组未见血栓形成。结论猕猴桃根多糖对角叉菜胶诱导的小鼠血栓形成具有一定的抑制作用,这可能与其抑制凝血途径有关。
Objective To explore the effect of Actinidia chinensis Planch. polysaccharide on carrageenan-induced tail thrombosis in mice. Methods Sixty mice were divided into control group,model group,aspirin group,low-dose[40 mg/( kg · d) ],medium-dose[80 mg/( kg·d) ]and high-dose[160 mg/( kg·d) ] Actinidia chinensis Planch. polysaccharide groups,with 10 mice in each group.Except the control group and model group,the mice in each group were given corresponding drugs by gavage for 10 days. Except the control group,the mice in each group received intraperitoneal injection of carrageenan to develop the mouse model of tail thrombosis after 1 h of intragastric perfusion. After 24 h,relative average tail length( RATL) and inhibitory rate of RATL were calculated and blood coagulation function was detected in each group. The tail tissues of the mice were obtained to observe the tail thrombosis. Results The incidence of thrombosis was 100% in the model group,while no black tail occurred in the mice of the control group and high-dose Actinidia chinensis Planch. polysaccharide group. RATL of the mice in the high-dose Actinidia chinensis Planch. polysaccharide group was shorter than that in the model group( P < 0. 05),and the inhibitory rates of RATL of the mice in the Actinidia chinensis Planch. polysaccharide groups were all higher than the rate in the aspirin group. Prothrombin time and activated partial thromboplastin time of the mice in the high-and medium-dose Actinidia chinensis Planch. polysaccharide groups were longer than those in the model group or aspirin group( all P < 0. 05). Pathological results showed that various degrees of thrombosis were observed in the tail veins of the mice in the model group,aspirin group,low-and medium-dose Actinidia chinensis Planch. polysaccharide groups,however,no thrombosis was found in the high-dose Actinidia chinensis Planch. polysaccharide group. Conclusion Actinidia chinensis Planch. polysaccharide has a certain inhibitory effects on the carrageenan-induced thrombosis in mice,which might be related to its inhibition on coagulation pathway.
Objective To explore the effect of Actinidia chinensis Planch. polysaccharide on carrageenan-induced tail thrombosis in mice. Methods Sixty mice were divided into control group,model group,aspirin group,low-dose[40 mg/( kg · d) ],medium-dose[80 mg/( kg·d) ]and high-dose[160 mg/( kg·d) ] Actinidia chinensis Planch. polysaccharide groups,with 10 mice in each group.Except the control group and model group,the mice in each group were given corresponding drugs by gavage for 10 days. Except the control group,the mice in each group received intraperitoneal injection of carrageenan to develop the mouse model of tail thrombosis after 1 h of intragastric perfusion. After 24 h,relative average tail length( RATL) and inhibitory rate of RATL were calculated and blood coagulation function was detected in each group. The tail tissues of the mice were obtained to observe the tail thrombosis. Results The incidence of thrombosis was 100% in the model group,while no black tail occurred in the mice of the control group and high-dose Actinidia chinensis Planch. polysaccharide group. RATL of the mice in the high-dose Actinidia chinensis Planch. polysaccharide group was shorter than that in the model group( P < 0. 05),and the inhibitory rates of RATL of the mice in the Actinidia chinensis Planch. polysaccharide groups were all higher than the rate in the aspirin group. Prothrombin time and activated partial thromboplastin time of the mice in the high-and medium-dose Actinidia chinensis Planch. polysaccharide groups were longer than those in the model group or aspirin group( all P < 0. 05). Pathological results showed that various degrees of thrombosis were observed in the tail veins of the mice in the model group,aspirin group,low-and medium-dose Actinidia chinensis Planch. polysaccharide groups,however,no thrombosis was found in the high-dose Actinidia chinensis Planch. polysaccharide group. Conclusion Actinidia chinensis Planch. polysaccharide has a certain inhibitory effects on the carrageenan-induced thrombosis in mice,which might be related to its inhibition on coagulation pathway.
引文
[1]冯国楣,李恒,徐祥浩.中国植物志[M].北京:科学出版社,1984:260.
[2]王仁才,熊兴耀,庞立.湖南猕猴桃产业发展的问题及建议[J].湖南农业科学,2015(5):124-127.
[3]江苏新医学院.中药大辞典[M].上海:上海科学技术出版社,1979:2 210-2 211.
[4]王小平,白吉庆,叶峥嵘.藤梨根提取物对裸鼠移植人胃癌SGC-7901细胞瘤p53基因蛋白的影响[J].现代中医药,2012,31(1):56-57.
[5]阎家麒,王九一,赵敏.中华猕猴桃多糖的提取及其对自由基的清除作用[J].中国生化药物杂志,1995,16(1):12-14.
[6]刘祝祥,尹红,肖琳莉,等.猕猴桃根多糖抗疲劳、抗氧化与单糖组分鉴定[J].食品科学,2013,34(13):239-242.
[7]李晓黎,戴文芸,石森林,等.猕猴桃根多糖脂质体对D-半乳糖胺所致小鼠实验性急性肝损伤的药效作用[J].中国临床药理学与治疗学,2014,19(6):611-614.
[8]张光霁,韩江余.猕猴桃根多糖抗肿瘤注射剂小鼠体内抑瘤机制[J].中华中医药杂志,2012,27(8):2 177-2 179.
[9]朱雨晴,石森林,吴瑾瑾,等.猕猴桃根多糖多相脂质体口服液对CCl4所致实验性急性肝损伤的药效作用[J].中华中医药学刊,2013,31(12):2 665-2 667,2 866.
[10]吴瑾瑾,叶丽美,邬云霞,等.猕猴桃根多糖的提取工艺研究[J].中华中医药学刊,2012,30(2):301-303.
[11]李加兴,李敏利,陈建伏,等.微波辅助提取猕猴桃根多糖工艺优化[J].食品科学,2010,31(4):42-45.
[12]赵洪,方萍.超声提取藤梨根多糖工艺研究[J].广州化工,2015,43(16):94-95,146.
[13]刘彦霞,赵肖萌,温宗妍,等.角叉菜胶两种给药方式制备小鼠尾部血栓模型比较[J].中西医结合心脑血管病杂志,2013,11(7):849-850.
[14]刘光荣,杨亚军,刘希望,等.角叉菜胶不同剂量对其诱导血栓模型建立的影响[J].中国临床药理学杂志,2016,32(24):2 293-2 296.
[15]刘彦霞,陈文,温宗妍,等.纳豆激酶对角叉菜胶所致小鼠血栓模型的影响[J].食品工业科技,2012,33(20):330-332,336.
[16]Bibbins-Domingo K,U.S.Preventive Services Task Force.Aspirin Use for the Primary Prevention of Cardiovascular Disease and Colorectal Cancer:US Preventive Services Task Force Recommendation Statement[J].Ann Intern Med,2016,164(12):836-845.
[17]Kearon C,Akl EA,Ornelas J,et al.Antithrombotic Therapy for VTE Disease:CHEST Guideline and Expert Panel Report[J].Chest,2016,149(2):315-352.
[18]Ryu HY,Ahn SM,Kim JS,et al.Evaluation of in-vitro Anticoagulation Activity of 33 Different Medicinal Herbs[J].J Life Sci,2010,20(6):922-928.
[19]刘瑜,董小黎.血栓动物模型的建立[J].首都医科大学学报,2002,23(3):277-280.
[20]魏陵博,戎冬梅,吉中强,等.角叉菜胶致大鼠尾部血栓形成的机制[J].中西医结合心脑血管病杂志,2008,6(5):542-543.
[2]王仁才,熊兴耀,庞立.湖南猕猴桃产业发展的问题及建议[J].湖南农业科学,2015(5):124-127.
[3]江苏新医学院.中药大辞典[M].上海:上海科学技术出版社,1979:2 210-2 211.
[4]王小平,白吉庆,叶峥嵘.藤梨根提取物对裸鼠移植人胃癌SGC-7901细胞瘤p53基因蛋白的影响[J].现代中医药,2012,31(1):56-57.
[5]阎家麒,王九一,赵敏.中华猕猴桃多糖的提取及其对自由基的清除作用[J].中国生化药物杂志,1995,16(1):12-14.
[6]刘祝祥,尹红,肖琳莉,等.猕猴桃根多糖抗疲劳、抗氧化与单糖组分鉴定[J].食品科学,2013,34(13):239-242.
[7]李晓黎,戴文芸,石森林,等.猕猴桃根多糖脂质体对D-半乳糖胺所致小鼠实验性急性肝损伤的药效作用[J].中国临床药理学与治疗学,2014,19(6):611-614.
[8]张光霁,韩江余.猕猴桃根多糖抗肿瘤注射剂小鼠体内抑瘤机制[J].中华中医药杂志,2012,27(8):2 177-2 179.
[9]朱雨晴,石森林,吴瑾瑾,等.猕猴桃根多糖多相脂质体口服液对CCl4所致实验性急性肝损伤的药效作用[J].中华中医药学刊,2013,31(12):2 665-2 667,2 866.
[10]吴瑾瑾,叶丽美,邬云霞,等.猕猴桃根多糖的提取工艺研究[J].中华中医药学刊,2012,30(2):301-303.
[11]李加兴,李敏利,陈建伏,等.微波辅助提取猕猴桃根多糖工艺优化[J].食品科学,2010,31(4):42-45.
[12]赵洪,方萍.超声提取藤梨根多糖工艺研究[J].广州化工,2015,43(16):94-95,146.
[13]刘彦霞,赵肖萌,温宗妍,等.角叉菜胶两种给药方式制备小鼠尾部血栓模型比较[J].中西医结合心脑血管病杂志,2013,11(7):849-850.
[14]刘光荣,杨亚军,刘希望,等.角叉菜胶不同剂量对其诱导血栓模型建立的影响[J].中国临床药理学杂志,2016,32(24):2 293-2 296.
[15]刘彦霞,陈文,温宗妍,等.纳豆激酶对角叉菜胶所致小鼠血栓模型的影响[J].食品工业科技,2012,33(20):330-332,336.
[16]Bibbins-Domingo K,U.S.Preventive Services Task Force.Aspirin Use for the Primary Prevention of Cardiovascular Disease and Colorectal Cancer:US Preventive Services Task Force Recommendation Statement[J].Ann Intern Med,2016,164(12):836-845.
[17]Kearon C,Akl EA,Ornelas J,et al.Antithrombotic Therapy for VTE Disease:CHEST Guideline and Expert Panel Report[J].Chest,2016,149(2):315-352.
[18]Ryu HY,Ahn SM,Kim JS,et al.Evaluation of in-vitro Anticoagulation Activity of 33 Different Medicinal Herbs[J].J Life Sci,2010,20(6):922-928.
[19]刘瑜,董小黎.血栓动物模型的建立[J].首都医科大学学报,2002,23(3):277-280.
[20]魏陵博,戎冬梅,吉中强,等.角叉菜胶致大鼠尾部血栓形成的机制[J].中西医结合心脑血管病杂志,2008,6(5):542-543.