芒花粉活力及柱头可授性研究
|
摘要
采用I2-KI染色法和离体萌发法测定芒(Miscanthus sinensis)花粉活力以及不同保存条件下的花粉寿命,并利用H2O2法检测雌蕊柱头的可授性。结果表明:芒花粉量大,平均花粉量/胚珠比(P/O值)达到5445±2883,属于专性异交性植物,且花粉初始活力较高,平均为78.68%±17.46%,但离体花粉的活力丧失快,室温干燥和湿润条件下保存的花粉寿命分别为70min和100min。不同基因型在花粉量、花粉活力及寿命上存在变异。雌蕊柱头可授性较高,活性可持续8d,最佳授粉期为开花前1d至开花后1d。
The pollen viability for different Miscanthus sinensis genotypes and the pollen life-span under dry or wet storage conditions were evaluated by in vitro pollen germination method and I2-KI staining method,and the stigma receptivity was also determined with H_2O_2 method.Results showed that the anther of M.sinensis had a large number of pollen grains with a mean pollen-ovule ratio(P/O)up to 5445±2883,belonging to a reproduction system of oligate xenogamy.The average initial pollen viability was 78.68% ±17.46%,but at room temperature(25℃),the pollen viability for all tested genotypes only lasted around 70 minutes under dry storage condition and 110 minutes under wet condition,respectively.The different genotypes kept a higher stigma receptivity until 8days post-anthesis(8DPA),and the optimal period to receive pollen grains was from-1 DPA to 1 DPA.
The pollen viability for different Miscanthus sinensis genotypes and the pollen life-span under dry or wet storage conditions were evaluated by in vitro pollen germination method and I2-KI staining method,and the stigma receptivity was also determined with H_2O_2 method.Results showed that the anther of M.sinensis had a large number of pollen grains with a mean pollen-ovule ratio(P/O)up to 5445±2883,belonging to a reproduction system of oligate xenogamy.The average initial pollen viability was 78.68% ±17.46%,but at room temperature(25℃),the pollen viability for all tested genotypes only lasted around 70 minutes under dry storage condition and 110 minutes under wet condition,respectively.The different genotypes kept a higher stigma receptivity until 8days post-anthesis(8DPA),and the optimal period to receive pollen grains was from-1 DPA to 1 DPA.
引文
[1]刘亮,朱明,朱太平.芒荻类植物资源的开发和利用[J].自然资源学报,2001,16(6):562-563.
[2]Zub H W,Brancourt-Hulmel M.Agronomic and physiological performances of different species of Miscanthus,a major energy crop.A review[J].Agronomy for Sustainable Development,2010,30(2):201-214.
[3]Chung J,Kim D.Miscanthus as a potential bioenergy crop in East Asia[J].Journal of Crop Science and Biotechnology,2012,15(2):65-77.
[4]文浩,艾辛,蒋建雄,等.采用SSR分子标记鉴定荻与南荻F1杂交种的真实性[J].草原与草坪,2013,33(4):7-11,22.
[5]梁绪振,陈太祥,白史且,等.芒属(Miscanthus)植物种质资源研究进展[J].草业与畜牧,2010(10):1-5.
[6]陈少风,何俊,周朴华,等.芒和五节芒的核型研究[J].江西农业大学学报,2008(1):123-126.
[7]于慧,胡晓颖,徐信兰,等.须芒草族植物花粉形态的观察[J].热带亚热带植物学报,2000(2):164-170.
[8]Collura S,Azanbre B,Finqueneisel G,et al.Miscanthus x giganteus straw and pellets as sustainable fuels:combination and emission tests[J].Environmental Chemistry Letters,2006,4(2):75-78.
[9]Heaton E A,Dohleman F G,Long S P.Meeting U.S.biofuel goals with less land:The potential of Miscanthus[J].Global Change Biology,2008,14(9):2000-2014.
[10]傅鲜桃,杨春华,陈灵鸷,等.“广益”扁穗牛鞭草花粉特性及结实性研究[J].草业学报,2008,17(2):61-76.
[11]赵金花,李青丰,那仁图雅,等.3种野生葱属植物花粉活力和柱头可授性研究[J].草业科学,2010,27(4):93-96.
[12]于晓英,卢向阳,龚明福,等.瓜叶菊花粉生活力研究[J].湖南农业大学学报(自然科学版),2005,31(1):42-46.
[13]胡适宜.植物胚胎学试验方法(一):花粉生活力的测定[J].植物学通报,1993,10(2):60-62.
[14]Dafni A,Kevan P G,Husband B C.Practical pollination biology[M].Cambridge,Ontarie,Canada:Enviroquest Ltd,2005:47-51.
[15]Cruden R W.Pollen-ovule ratios:a conservative indicator of breeding systems in flowering plants[J].Evolution,1977,31(1):32-46.
[16]孙光玲,汪银生,胡乾政.烟草花粉贮藏与活力测定[J].中国烟草,1994(1):20-22.
[17]李训贞,梁满中,周广洽,等.水稻开花时的环境条件对花粉活力和结实的影响[J].作物学报,2002,28(3):417-420.
[18]李要民,陈良碧.不同温湿条件下贮藏的3种禾本科植物花粉活力变化[J].植物生理学通讯,1998,34(1):35-37.
[19]Rêgo M M,Rêgo E R,Bruckner C H,et al.Overcoming self-incompatibility in passion fruit by double pollination in anthesis stages[J].Acta Horticulturae,2013,1000:533-536.
[20]Gradziel T M,Robinson R W.Breakdown of self-incompatibility during pistil development in Lycopersicon peruvianum by modified bud pollination[J].Sexual Plant Reproduction,1989,2(1):38-42.
[2]Zub H W,Brancourt-Hulmel M.Agronomic and physiological performances of different species of Miscanthus,a major energy crop.A review[J].Agronomy for Sustainable Development,2010,30(2):201-214.
[3]Chung J,Kim D.Miscanthus as a potential bioenergy crop in East Asia[J].Journal of Crop Science and Biotechnology,2012,15(2):65-77.
[4]文浩,艾辛,蒋建雄,等.采用SSR分子标记鉴定荻与南荻F1杂交种的真实性[J].草原与草坪,2013,33(4):7-11,22.
[5]梁绪振,陈太祥,白史且,等.芒属(Miscanthus)植物种质资源研究进展[J].草业与畜牧,2010(10):1-5.
[6]陈少风,何俊,周朴华,等.芒和五节芒的核型研究[J].江西农业大学学报,2008(1):123-126.
[7]于慧,胡晓颖,徐信兰,等.须芒草族植物花粉形态的观察[J].热带亚热带植物学报,2000(2):164-170.
[8]Collura S,Azanbre B,Finqueneisel G,et al.Miscanthus x giganteus straw and pellets as sustainable fuels:combination and emission tests[J].Environmental Chemistry Letters,2006,4(2):75-78.
[9]Heaton E A,Dohleman F G,Long S P.Meeting U.S.biofuel goals with less land:The potential of Miscanthus[J].Global Change Biology,2008,14(9):2000-2014.
[10]傅鲜桃,杨春华,陈灵鸷,等.“广益”扁穗牛鞭草花粉特性及结实性研究[J].草业学报,2008,17(2):61-76.
[11]赵金花,李青丰,那仁图雅,等.3种野生葱属植物花粉活力和柱头可授性研究[J].草业科学,2010,27(4):93-96.
[12]于晓英,卢向阳,龚明福,等.瓜叶菊花粉生活力研究[J].湖南农业大学学报(自然科学版),2005,31(1):42-46.
[13]胡适宜.植物胚胎学试验方法(一):花粉生活力的测定[J].植物学通报,1993,10(2):60-62.
[14]Dafni A,Kevan P G,Husband B C.Practical pollination biology[M].Cambridge,Ontarie,Canada:Enviroquest Ltd,2005:47-51.
[15]Cruden R W.Pollen-ovule ratios:a conservative indicator of breeding systems in flowering plants[J].Evolution,1977,31(1):32-46.
[16]孙光玲,汪银生,胡乾政.烟草花粉贮藏与活力测定[J].中国烟草,1994(1):20-22.
[17]李训贞,梁满中,周广洽,等.水稻开花时的环境条件对花粉活力和结实的影响[J].作物学报,2002,28(3):417-420.
[18]李要民,陈良碧.不同温湿条件下贮藏的3种禾本科植物花粉活力变化[J].植物生理学通讯,1998,34(1):35-37.
[19]Rêgo M M,Rêgo E R,Bruckner C H,et al.Overcoming self-incompatibility in passion fruit by double pollination in anthesis stages[J].Acta Horticulturae,2013,1000:533-536.
[20]Gradziel T M,Robinson R W.Breakdown of self-incompatibility during pistil development in Lycopersicon peruvianum by modified bud pollination[J].Sexual Plant Reproduction,1989,2(1):38-42.