摘要
季铵盐类聚合物抗菌剂(PQACs)相比较于传统的纳米金属离子抗菌剂和天然抗菌剂,不仅没有生物毒性,而且具有抗菌效果更长效更广谱的特点。因此为了制备出具有强杀菌效果的PQACs,通过采用辅助活化还原原子转移自由基聚合(SARA ATRP)方法制备(3-丙烯酰胺丙基)三甲基氯化铵(AMPTMA)和聚乙二醇(PEG)的季铵盐类共聚合物抗菌剂。通过核磁共振(~1H NMR)、红外光谱(FT-IR)、凝胶渗透色谱(GPC)分别对其进行结构表征;并初步探讨了不同浓度同种聚合物抗菌剂以及同种浓度不同抗菌剂对于大肠杆菌以及金黄色葡萄球菌的抗菌性能,测试结果表明,在大肠杆菌的抗菌和金黄色葡萄球菌抗菌性能测试中,聚合物抗菌剂的抗菌性能都随着所测浓度的增加而提高;且抗菌聚合物分子质量越大抗菌性能越好;最后,采用MTT法对抗菌剂进行细胞毒性测试,结果表明抗菌剂的细胞毒性会随着其浓度的增加而加大。
Quaternary ammonium polymer antibacterial agents (PQACs)are not only biocompatible,but also have longer and broader spectrum of antibacterial property,compared to the traditional metallic ions nano particles and natural antibacterial agents.Therefore,in order to prepare PQACs with a strong bactericidal property,this article described the synthesis of a series of quaternary ammoniums based on supplemental activator and reducing agent atom transfer radical polymerization (SARA ATRP)to prepare (3-acrylamidopropyl)trimethy-lammonium chloride (AMPTMA)-polyethylene glycol (PEG)copolymers as antibacterial agents.The structure of synthesis antibacterial polyamers were characterized by ~1H NMR,FT-IR,and gel permeation chromatography (GPC).Furthermore,the antibacterial properties about different concentrations of the polymers for E.coli and Staphylococcus aureus were discussed.The higher antibacterial efficiency of the polymer antibacterial with the increase of the measured polymer concentration,the larger the antibacterial polymer molecular weight,the better the antibacterial property.Finally,the MTT assay shows the cytotoxicity of the antibacterial agent increased with its concentration increased.
引文
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