Identification of the resistance gene to powdery mildew in Chinese wheat landrace Baiyouyantiao
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摘要
Powdery mildew,caused by Blumeria graminis f.sp.tritici(Bgt),is one of the most damaging diseases to wheat in the world.The cultivation of resistant varieties of wheat is essential for controlling the powdery mildew epidemic.Wheat landraces are important resources of resistance to many diseases.Mapping powdery mildew resistance genes from wheat landraces will promote the development of new varieties with disease resistance.The Chinese wheat landrace Baiyouyantiao possesses characteristic of disease resistance to powdery mildew.To identify the resistance gene in this landrace,Baiyouyantiao was crossed with the susceptible cultivar Jingshuang 16 and seedlings of parents and F_1,BC_1,F_2,and F_(2:3) were tested with Bgt isolate E09.The genetic results showed that the resistance of Baiyouyantiao to E09 was controlled by a single recessive gene,tentatively designated Pm BYYT.An Illumina wheat 90K single-nucleotide polymorphism(SNP)array was applied to screen polymorphisms between F_2-resistant and F_2-susceptible DNA bulks for identifying the chromosomal location of Pm BYYT.A high percentage of polymorphic SNPs between the resistant and susceptible DNA bulks was found on chromosome 7B,indicating that Pm BYYT may be located on this chromosome.A genetic linkage map of Pm BYYT consisting of two simple sequence repeat markers and eight SNP markers was developed.The two flanking markers were SNP markers W7BL-8 and W7BL-15,with genetic distances of 3 and 2.9 c M,respectively.The results of this study demonstrated the rapid characterization of a wheat disease resistance gene and SNP marker development using the 90K SNP assay.The flanking markers of gene Pm BYYT will benefit marker-assisted selection(MAS)and map-based cloning in breeding wheat cultivars with powdery mildew resistance.
Powdery mildew,caused by Blumeria graminis f.sp.tritici(Bgt),is one of the most damaging diseases to wheat in the world.The cultivation of resistant varieties of wheat is essential for controlling the powdery mildew epidemic.Wheat landraces are important resources of resistance to many diseases.Mapping powdery mildew resistance genes from wheat landraces will promote the development of new varieties with disease resistance.The Chinese wheat landrace Baiyouyantiao possesses characteristic of disease resistance to powdery mildew.To identify the resistance gene in this landrace,Baiyouyantiao was crossed with the susceptible cultivar Jingshuang 16 and seedlings of parents and F_1,BC_1,F_2,and F_(2:3) were tested with Bgt isolate E09.The genetic results showed that the resistance of Baiyouyantiao to E09 was controlled by a single recessive gene,tentatively designated Pm BYYT.An Illumina wheat 90K single-nucleotide polymorphism(SNP)array was applied to screen polymorphisms between F_2-resistant and F_2-susceptible DNA bulks for identifying the chromosomal location of Pm BYYT.A high percentage of polymorphic SNPs between the resistant and susceptible DNA bulks was found on chromosome 7B,indicating that Pm BYYT may be located on this chromosome.A genetic linkage map of Pm BYYT consisting of two simple sequence repeat markers and eight SNP markers was developed.The two flanking markers were SNP markers W7BL-8 and W7BL-15,with genetic distances of 3 and 2.9 c M,respectively.The results of this study demonstrated the rapid characterization of a wheat disease resistance gene and SNP marker development using the 90K SNP assay.The flanking markers of gene Pm BYYT will benefit marker-assisted selection(MAS)and map-based cloning in breeding wheat cultivars with powdery mildew resistance.
Powdery mildew,caused by Blumeria graminis f.sp.tritici(Bgt),is one of the most damaging diseases to wheat in the world.The cultivation of resistant varieties of wheat is essential for controlling the powdery mildew epidemic.Wheat landraces are important resources of resistance to many diseases.Mapping powdery mildew resistance genes from wheat landraces will promote the development of new varieties with disease resistance.The Chinese wheat landrace Baiyouyantiao possesses characteristic of disease resistance to powdery mildew.To identify the resistance gene in this landrace,Baiyouyantiao was crossed with the susceptible cultivar Jingshuang 16 and seedlings of parents and F_1,BC_1,F_2,and F_(2:3) were tested with Bgt isolate E09.The genetic results showed that the resistance of Baiyouyantiao to E09 was controlled by a single recessive gene,tentatively designated Pm BYYT.An Illumina wheat 90K single-nucleotide polymorphism(SNP)array was applied to screen polymorphisms between F_2-resistant and F_2-susceptible DNA bulks for identifying the chromosomal location of Pm BYYT.A high percentage of polymorphic SNPs between the resistant and susceptible DNA bulks was found on chromosome 7B,indicating that Pm BYYT may be located on this chromosome.A genetic linkage map of Pm BYYT consisting of two simple sequence repeat markers and eight SNP markers was developed.The two flanking markers were SNP markers W7BL-8 and W7BL-15,with genetic distances of 3 and 2.9 c M,respectively.The results of this study demonstrated the rapid characterization of a wheat disease resistance gene and SNP marker development using the 90K SNP assay.The flanking markers of gene Pm BYYT will benefit marker-assisted selection(MAS)and map-based cloning in breeding wheat cultivars with powdery mildew resistance.
引文
Brenchley R,Spannagl M,Pfeifer M,Barker G L,D’Amore R,Allen A M,Mc Kenzie N,Kramer M,Kerhornou A,Bolser D,Kay S,Waite D,Trick M,Bancroft I,Gu Y,Huo N,Luo M C,Sehgal S,Gill B,Kianian S,et al.2012.Analysis of the bread wheat genome using whole-genome shotgun sequencing.Nature,491,705-710.
Dreisigacker S,Wang X,Martinez Cisneros B A,Jing R,Singh P K.2015.Adult-plant resistance to Septoria tritici blotch in hexaploid spring wheat.Theoretical and Applied Genetics,128,2317-2329.
Hao Y F,Liu A F,Wang Y H,Feng D S,Gao J R,Li X F,Liu S B,Wang H G.2008.Pm23:A new allele of Pm4 located on chromosome 2AL in wheat.Theoretical and Applied Genetics,117,1205-1212.
Hartl L,Weiss H,Stephan U,Zeller F J,Jahoor A.1995.Molecular identification of powdery mildew resistance genes in common wheat(Triticum aestivum L.).Theoretical and Applied Genetics,90,601-606.
Hsam S L K,Huang X Q,Zeller F J.2001.Chromosomal location of genes for resistance to powdery mildew in common wheat(Triticum aestivum L.em Thell.)6.Alleles at the Pm5 locus.Theoretical and Applied Genetics,102,127-133.
Hu T Z,Li H J,Xie C J,You M S,Yang Z M,Sun Q X,Liu ZY.2008.Molecular mapping and chromosomal location of powdery mildew resistance gene in wheat variety Tangmai 4.Acta Agronomica Sinica,34,1193-1198.(in Chinese)
Hua W,Liu Z J,Zhu J,Xie C J,Yang T,Zhou Y L,Duan X Y,Sun Q X,Liu Z Y.2009.Identification and genetic mapping of pm42,a new recessive wheat powdery mildew resistance gene derived from wild emmer(Triticum turgidum var.dicoccoides).Theoretical and Applied Genetics,119,223-230.
Huang X Q,Hsam S L K,Zeller F J.2000.Chromosomal location of powdery mildew resistance genes in Chinese wheat(Triticum aestivum L.em.Thell.)landraces Xiaobaidong and Fuzhuang 30.Journal of Genetics and Breeding,54,311-317.
Huang X Q,R?der M S.2011.High-density genetic and physical bin mapping of wheat chromosome 1D reveals that the powdery mildew resistance gene Pm24 is located in a highly recombinogenic region.Genetica,139,1179-1187.
Huang X Q,Wang L X,Xu M X,R?der M.2003.Microsatellite mapping of the powdery mildew resistance gene Pm5e in common wheat(Triticum aestivum L.).Theoretical and Applied Genetics,106,858-865.
IWGSC(International Wheat Genome Sequencing Consortium),David E,Jacqueline B.2014.A chromosome-based draft sequence of the hexaploid bread wheat(Triticum aestivum)genome.Science,345,e1251788.
Jia J Z,Zhao S C,Kong X Y,Li Y R,Zhao G Y,He W M,Appels R,Pfeifer M,Tao Y,Zhang X Y,Jing R L,Zhang C,Ma YZ,Gao L F,Gao C,Spannagl M,Mayer K F X,Li D,Pan SK,Zheng F Y,et al.2013.Aegilops tauschii draft genome sequence reveals a gene repertoire for wheat adaptation.Nature,496,91-95.
Kosambi D D.1943.The estimation of map distances from recombination values.Annals of Human Genetics,12,172-175.
Law C N,Wolfe M S.1966.Location of genetic factors for mildew resistance and ear emergence time on chromosome7B of wheat.Canadian Journal of Genetics and Cytology,8,462-470.
Liao Y,Sun B J,Sun G W,Liu H C,Chen R Y.2009.The application and key problems of bulked segregant analysis on the research of molecular marker in crop.Molecular Plant Breeding,7,162-168.
Ling H Q,Zhao S,Liu D,Wang J Y,Sun H,Zhang C,Fan HJ,Li D,Dong L L,Tao Y,Gao C,Wu H L,Li Y W,Cui Y,Guo X S,Zheng S S,Wang B,Yu K,Liang Q S,Yang W L,et al.2013.Draft genome of the wheat A-genome progenitor Triticum urartu.Nature,496,87-90.
Liu K,Deng Z Y,Li Q F,Zhang Y,Sun C L,Tian J C,Chen JS.2016.Mapping QTLs for wheat panicle traits with high density SNP genetic map.Acta Agronomica Sinica,42,820-831.(in Chinese)
Liu R H,Meng J L.2003.Map Draw:A Microsoft Excel macro for drawing genetic linkage maps based on given genetic linkage data.Heraditas(Beijing),25,317-321.(in Chinese)
Liu S C,Zheng D S,Cao Y S,Song C H,Chen M Y.2000.Genetic diversity of landrace and bred varieties of wheat in China.Scientia Agricultura Sinica,33,20-24.(in Chinese)
Lu Y Q,Wu X Y,Yao M M,Zhang J P,Liu W H,Yang X M,Li X Q,Du J,Gao A N,Li L H.2015.Genetic mapping of a putative Agropyron cristatum-derived powdery mildew resistance gene by a combination of bulked segregant analysis and single nucleotide polymorphism array.Molecular Breeding,35,96.
Luo P G,Luo H Y,Chang Z J,Zhang H Y,Zhang M,Ren Z L.2009.Characterization and chromosomal location of Pm40in common wheat:A new gene for resistance to powdery mildew derived from Elytrigia intermedium.Theoretical and Applied Genetics,118,1059-1064.
Miranda L M,Murphy J P,Marshall D,Cowger C,Leath S.2007.Chromosomal location of Pm35,a novel Aegilops tauschii derived powdery mildew resistance gene introgressed into common wheat(Triticum aestivum L.).Theoretical and Applied Genetics,114,1451-1456.
Petersen S,Lyerly J H,Worthington M L,Parks W R,Cowger C,Marshall D S,Brown-Guedira G,Murphy J P.2015.Mapping of powdery mildew resistance gene Pm53 introgressed from Aegilops speltoides into soft red winter wheat.Theoretical and Applied Genetics,128,303-312.
Rogers S O,Bendich A J.1985.Extraction of DNA from milligram amounts of fresh,herbarium and mummified plant tissues.Plant Molecular Biology,5,69-76.
Sheng B Q.1988.Wheat powdery mildew was recorded using infection type in seedling stage.Plant Protection,1,49.(in Chinese)
Sheng B Q,Duan X Y,Zhou Y L,Wang J X.1992.Cluster of powdery mildew resistance genes carried in some Chinese wheat landraces.Crop Genetic Resources,4,33-35.(in Chinese)
Singrün C,Hsam S L K,Hartl L,Zeller F J,Mohler V.2003.Powdery mildew resistance gene Pm22 in cultivar Virest is a member of the complex Pm1 locus in common wheat(Triticum aestivum L.em Thell.).Theoretical and Applied Genetics,106,1420-1424.
Tao W,Liu D,Liu J,Feng Y,Chen P.2000.Genetic mapping of the powdery mildew resistance gene Pm6 in wheat by RFLPanalysis.Theoretical and Applied Genetics,100,564-568.
Wang J M,Kang Z S,Liu H Y.2009.Genetic analysis and location of a powdery mildew resistance gene in wheat landrace Hongyoumai by SSR marker.Acta Phytopathologica Sinica,39,285-289.(in Chinese)
Wang S,Wong D,Forrest K,Allen A,Chao S,Huang B E,Maccaferri M,Salvi S,Milner S G,Cattivelli L,Mastrangelo A M,Whan A,Stephen S,Barker G,Wieseke R,Plieske J,IWGSC(International Wheat Genome Sequencing Consortium),Lillemo M,Mather D,Appels R,et al.2014.Characterization of polyploid wheat genomic diversity using a high-density 90 000 single nucleotide polymorphism array.Plant Biotechnology Journal,12,787-796.
Wang Z Z,Li H W,Zhang D Y,Guo L,Chen J J,Chen Y X,Wu Q H,Xie J Z,Zhang Y,Sun Q X,Dvorak J,Luo M C,Liu Z Y.2015.Genetic and physical mapping of powdery mildew resistance gene Ml HLT in Chinese wheat landrace Hulutou.Theoretical and Applied Genetics,128,365-373.
Xiao M G,Song F J,Jiao J L,Wang X M,Xu H X,Li H J.2013.Identification of the gene Pm47 on chromosome 7BSconferring resistance to powdery mildew in the Chinese wheat landrace Hongyanglazi.Theoretical and Applied Genetics,126,1397-1403.
Xue F,Duan X Y,Zhou Y L,Ji W Q.2009a.Postulation of powdery mildew resistance genes carried in some Chinese wheat landraces and the genetic diversity analysis.Journal of Triticeae Crops,29,228-235.(in Chinese)
Xue F,Zhai W W,Duan X Y,Zhou Y L,Ji W Q.2009b.Microsatellite mapping of powdery mildew resistance gene in wheat landrace Xiaobaidong.Acta Agronomica Sinica,35,1806-1811.(in Chinese)
Yu H X,Deng Z Y,Xiang C,Tian J C.2014.Analysis of diversity and linkage disequilibrium mapping of agronomic traits on B-genome of wheat.Journal of Genomics,2,20-30.
Zanke C,Ling J,Plieske J,Kollers S,Ebmeyer E,Korzun V,Argillier O,Stiewe G,Hinze M,Beier S,Ganal M W,R?der M S.2014.Genetic architecture of main effect QTL for heading date in European winter wheat.Frontiers in Plant Science,5,1-12.
Zhang Q L,Gu F,Li T,Gao J R,Wang H G.2004.Identification of co-segregating RAPD marker linked to powdery mildew resistance gene Pm18 in wheat.Agriculture Sciences in China,3,409-415.
Zhang R,Sun B,Chen J,Cao A,Xing L,Feng Y,Lan C,Chen P.2016.Pm55,a developmental-stage and tissuespecific powdery mildew resistance gene introgressed from Dasypyrum villosum into common wheat.Theoretical and Applied Genetics,129,1975-1984.
Dreisigacker S,Wang X,Martinez Cisneros B A,Jing R,Singh P K.2015.Adult-plant resistance to Septoria tritici blotch in hexaploid spring wheat.Theoretical and Applied Genetics,128,2317-2329.
Hao Y F,Liu A F,Wang Y H,Feng D S,Gao J R,Li X F,Liu S B,Wang H G.2008.Pm23:A new allele of Pm4 located on chromosome 2AL in wheat.Theoretical and Applied Genetics,117,1205-1212.
Hartl L,Weiss H,Stephan U,Zeller F J,Jahoor A.1995.Molecular identification of powdery mildew resistance genes in common wheat(Triticum aestivum L.).Theoretical and Applied Genetics,90,601-606.
Hsam S L K,Huang X Q,Zeller F J.2001.Chromosomal location of genes for resistance to powdery mildew in common wheat(Triticum aestivum L.em Thell.)6.Alleles at the Pm5 locus.Theoretical and Applied Genetics,102,127-133.
Hu T Z,Li H J,Xie C J,You M S,Yang Z M,Sun Q X,Liu ZY.2008.Molecular mapping and chromosomal location of powdery mildew resistance gene in wheat variety Tangmai 4.Acta Agronomica Sinica,34,1193-1198.(in Chinese)
Hua W,Liu Z J,Zhu J,Xie C J,Yang T,Zhou Y L,Duan X Y,Sun Q X,Liu Z Y.2009.Identification and genetic mapping of pm42,a new recessive wheat powdery mildew resistance gene derived from wild emmer(Triticum turgidum var.dicoccoides).Theoretical and Applied Genetics,119,223-230.
Huang X Q,Hsam S L K,Zeller F J.2000.Chromosomal location of powdery mildew resistance genes in Chinese wheat(Triticum aestivum L.em.Thell.)landraces Xiaobaidong and Fuzhuang 30.Journal of Genetics and Breeding,54,311-317.
Huang X Q,R?der M S.2011.High-density genetic and physical bin mapping of wheat chromosome 1D reveals that the powdery mildew resistance gene Pm24 is located in a highly recombinogenic region.Genetica,139,1179-1187.
Huang X Q,Wang L X,Xu M X,R?der M.2003.Microsatellite mapping of the powdery mildew resistance gene Pm5e in common wheat(Triticum aestivum L.).Theoretical and Applied Genetics,106,858-865.
IWGSC(International Wheat Genome Sequencing Consortium),David E,Jacqueline B.2014.A chromosome-based draft sequence of the hexaploid bread wheat(Triticum aestivum)genome.Science,345,e1251788.
Jia J Z,Zhao S C,Kong X Y,Li Y R,Zhao G Y,He W M,Appels R,Pfeifer M,Tao Y,Zhang X Y,Jing R L,Zhang C,Ma YZ,Gao L F,Gao C,Spannagl M,Mayer K F X,Li D,Pan SK,Zheng F Y,et al.2013.Aegilops tauschii draft genome sequence reveals a gene repertoire for wheat adaptation.Nature,496,91-95.
Kosambi D D.1943.The estimation of map distances from recombination values.Annals of Human Genetics,12,172-175.
Law C N,Wolfe M S.1966.Location of genetic factors for mildew resistance and ear emergence time on chromosome7B of wheat.Canadian Journal of Genetics and Cytology,8,462-470.
Liao Y,Sun B J,Sun G W,Liu H C,Chen R Y.2009.The application and key problems of bulked segregant analysis on the research of molecular marker in crop.Molecular Plant Breeding,7,162-168.
Ling H Q,Zhao S,Liu D,Wang J Y,Sun H,Zhang C,Fan HJ,Li D,Dong L L,Tao Y,Gao C,Wu H L,Li Y W,Cui Y,Guo X S,Zheng S S,Wang B,Yu K,Liang Q S,Yang W L,et al.2013.Draft genome of the wheat A-genome progenitor Triticum urartu.Nature,496,87-90.
Liu K,Deng Z Y,Li Q F,Zhang Y,Sun C L,Tian J C,Chen JS.2016.Mapping QTLs for wheat panicle traits with high density SNP genetic map.Acta Agronomica Sinica,42,820-831.(in Chinese)
Liu R H,Meng J L.2003.Map Draw:A Microsoft Excel macro for drawing genetic linkage maps based on given genetic linkage data.Heraditas(Beijing),25,317-321.(in Chinese)
Liu S C,Zheng D S,Cao Y S,Song C H,Chen M Y.2000.Genetic diversity of landrace and bred varieties of wheat in China.Scientia Agricultura Sinica,33,20-24.(in Chinese)
Lu Y Q,Wu X Y,Yao M M,Zhang J P,Liu W H,Yang X M,Li X Q,Du J,Gao A N,Li L H.2015.Genetic mapping of a putative Agropyron cristatum-derived powdery mildew resistance gene by a combination of bulked segregant analysis and single nucleotide polymorphism array.Molecular Breeding,35,96.
Luo P G,Luo H Y,Chang Z J,Zhang H Y,Zhang M,Ren Z L.2009.Characterization and chromosomal location of Pm40in common wheat:A new gene for resistance to powdery mildew derived from Elytrigia intermedium.Theoretical and Applied Genetics,118,1059-1064.
Miranda L M,Murphy J P,Marshall D,Cowger C,Leath S.2007.Chromosomal location of Pm35,a novel Aegilops tauschii derived powdery mildew resistance gene introgressed into common wheat(Triticum aestivum L.).Theoretical and Applied Genetics,114,1451-1456.
Petersen S,Lyerly J H,Worthington M L,Parks W R,Cowger C,Marshall D S,Brown-Guedira G,Murphy J P.2015.Mapping of powdery mildew resistance gene Pm53 introgressed from Aegilops speltoides into soft red winter wheat.Theoretical and Applied Genetics,128,303-312.
Rogers S O,Bendich A J.1985.Extraction of DNA from milligram amounts of fresh,herbarium and mummified plant tissues.Plant Molecular Biology,5,69-76.
Sheng B Q.1988.Wheat powdery mildew was recorded using infection type in seedling stage.Plant Protection,1,49.(in Chinese)
Sheng B Q,Duan X Y,Zhou Y L,Wang J X.1992.Cluster of powdery mildew resistance genes carried in some Chinese wheat landraces.Crop Genetic Resources,4,33-35.(in Chinese)
Singrün C,Hsam S L K,Hartl L,Zeller F J,Mohler V.2003.Powdery mildew resistance gene Pm22 in cultivar Virest is a member of the complex Pm1 locus in common wheat(Triticum aestivum L.em Thell.).Theoretical and Applied Genetics,106,1420-1424.
Tao W,Liu D,Liu J,Feng Y,Chen P.2000.Genetic mapping of the powdery mildew resistance gene Pm6 in wheat by RFLPanalysis.Theoretical and Applied Genetics,100,564-568.
Wang J M,Kang Z S,Liu H Y.2009.Genetic analysis and location of a powdery mildew resistance gene in wheat landrace Hongyoumai by SSR marker.Acta Phytopathologica Sinica,39,285-289.(in Chinese)
Wang S,Wong D,Forrest K,Allen A,Chao S,Huang B E,Maccaferri M,Salvi S,Milner S G,Cattivelli L,Mastrangelo A M,Whan A,Stephen S,Barker G,Wieseke R,Plieske J,IWGSC(International Wheat Genome Sequencing Consortium),Lillemo M,Mather D,Appels R,et al.2014.Characterization of polyploid wheat genomic diversity using a high-density 90 000 single nucleotide polymorphism array.Plant Biotechnology Journal,12,787-796.
Wang Z Z,Li H W,Zhang D Y,Guo L,Chen J J,Chen Y X,Wu Q H,Xie J Z,Zhang Y,Sun Q X,Dvorak J,Luo M C,Liu Z Y.2015.Genetic and physical mapping of powdery mildew resistance gene Ml HLT in Chinese wheat landrace Hulutou.Theoretical and Applied Genetics,128,365-373.
Xiao M G,Song F J,Jiao J L,Wang X M,Xu H X,Li H J.2013.Identification of the gene Pm47 on chromosome 7BSconferring resistance to powdery mildew in the Chinese wheat landrace Hongyanglazi.Theoretical and Applied Genetics,126,1397-1403.
Xue F,Duan X Y,Zhou Y L,Ji W Q.2009a.Postulation of powdery mildew resistance genes carried in some Chinese wheat landraces and the genetic diversity analysis.Journal of Triticeae Crops,29,228-235.(in Chinese)
Xue F,Zhai W W,Duan X Y,Zhou Y L,Ji W Q.2009b.Microsatellite mapping of powdery mildew resistance gene in wheat landrace Xiaobaidong.Acta Agronomica Sinica,35,1806-1811.(in Chinese)
Yu H X,Deng Z Y,Xiang C,Tian J C.2014.Analysis of diversity and linkage disequilibrium mapping of agronomic traits on B-genome of wheat.Journal of Genomics,2,20-30.
Zanke C,Ling J,Plieske J,Kollers S,Ebmeyer E,Korzun V,Argillier O,Stiewe G,Hinze M,Beier S,Ganal M W,R?der M S.2014.Genetic architecture of main effect QTL for heading date in European winter wheat.Frontiers in Plant Science,5,1-12.
Zhang Q L,Gu F,Li T,Gao J R,Wang H G.2004.Identification of co-segregating RAPD marker linked to powdery mildew resistance gene Pm18 in wheat.Agriculture Sciences in China,3,409-415.
Zhang R,Sun B,Chen J,Cao A,Xing L,Feng Y,Lan C,Chen P.2016.Pm55,a developmental-stage and tissuespecific powdery mildew resistance gene introgressed from Dasypyrum villosum into common wheat.Theoretical and Applied Genetics,129,1975-1984.