建国以来黑龙江省春小麦抗白粉病基因检测及其组成分析
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摘要
为了解黑龙江省春小麦抗白粉病基因的组成及分布规律,本研究利用已开发的Pm2、Pm3b、Pm4、Pm8、Pm13和Pm21抗白粉病基因分子标记对黑龙江省推广的123份小麦品种进行了检测和分析。结果表明,在123份小麦品种中分布4种抗白粉病基因,其中Pm2、Pm3和Pm13分布频率较高,分别为95.1%、95.1%和94.3%,Pm8分布频率较低,仅为0.01%,且不存在抗白粉病基因Pm4和Pm21;黑龙江省春小麦抗白粉病基因组合共有7种类型,其中Pm2/Pm3b/Pm13类型所占比例最高,为88.6%,其次为Pm2/Pm3b、Pm2/Pm13和Pm3b/Pm13,所占比例分别为3.3%、2.4%和2.4%,Pm2和Pm2/Pm3b/Pm8/Pm13所占比例最低,为0.8%。本试验结果明确了建国后黑龙江省春小麦品种抗白粉病基因的组成和分布频率,为高效开展小麦抗白粉病育种提供了一定的理论依据。
To understand the composition and distribution of the powdery mildew resistance genes, a total of 123 spring wheat cultivars released in Heilongjiang province since the founding of new China were detected and analyzed with developed molecular markers, such as Pm2、Pm3b、Pm4、Pm8、Pm13 and Pm21. The results showed that there were four the powdery mildew resistance genes among 123 spring wheat cultivars.Pm2, Pm3 and Pm13 had the higher distribution freguency of 95.1%, 95.1% and 94.3%, respectively. Pm8 had the lower distribution frequency of 0.01% and Pm4 and Pm21 were not found in these spring wheat cultivars. There were seven combinations of powdery mildew resistance gene among Heilongjiang spring wheat cultivars. The combination of Pm2/Pm3b/Pm13(88.6%) had the highest frequency, followed by Pm2/Pm3 b(3.3%), Pm2/Pm13(2.4%) and Pm3b/Pm13(2.4%), and both Pm2 and Pm2/Pm3b/Pm8/Pm13 showed low frequency(0.8%). The study defined the composition and distribution frequency of the powdery mildew resistance genes in Heilongjiang spring wheat cultivars since the founding of new China and provided theoretical guidance for developing efficiently powdery mildew resistance breeding in wheat.
To understand the composition and distribution of the powdery mildew resistance genes, a total of 123 spring wheat cultivars released in Heilongjiang province since the founding of new China were detected and analyzed with developed molecular markers, such as Pm2、Pm3b、Pm4、Pm8、Pm13 and Pm21. The results showed that there were four the powdery mildew resistance genes among 123 spring wheat cultivars.Pm2, Pm3 and Pm13 had the higher distribution freguency of 95.1%, 95.1% and 94.3%, respectively. Pm8 had the lower distribution frequency of 0.01% and Pm4 and Pm21 were not found in these spring wheat cultivars. There were seven combinations of powdery mildew resistance gene among Heilongjiang spring wheat cultivars. The combination of Pm2/Pm3b/Pm13(88.6%) had the highest frequency, followed by Pm2/Pm3 b(3.3%), Pm2/Pm13(2.4%) and Pm3b/Pm13(2.4%), and both Pm2 and Pm2/Pm3b/Pm8/Pm13 showed low frequency(0.8%). The study defined the composition and distribution frequency of the powdery mildew resistance genes in Heilongjiang spring wheat cultivars since the founding of new China and provided theoretical guidance for developing efficiently powdery mildew resistance breeding in wheat.
引文
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[2] 赵远玲, 李祥羽, 孙连发, 陈立军. 人工合成小麦抗白粉病未知基因的SSR标记[J]. 植物遗传资源学报, 2011, 12(2):271-274
[3] 张增艳, 陈孝, 张超, 辛志勇, 陈新民. 分子标记选择小麦抗白粉病基因Pm4b、Pm13和Pm21聚合体[J]. 中国农业科学, 2002, 35(7):789-793
[4] 王玉叶, 张海萍, 潘艳秋, 常成, 马传喜. 96份小麦品种资源抗白粉病基因的分子检测[J]. 种子, 2013, 5(32):20-24
[5] 李祥羽, 孙连发, 陈立君, 宋凤英, 赵远玲. 小麦白粉病抗源筛选及抗源分布规律研究[J]. 黑龙江农业科学, 2009, 3(1):45-46
[6] 王丹, 赵继文, 秦海英, 谢文芳, 程星, 刘然方. 小麦抗白粉病基因定位及分子标记研究进展[J]. 农业科技通讯, 2013, 5(1):30-34
[7] 张宏纪, 刁艳玲, 孙连发, 孙岩, 刘东军, 郭强, 兰静, 黄景华, 杨淑萍, 孙光祖. 航天诱变新品种龙辐麦18的选育及其主要特征特性分析[J]. 核农学报, 2008, 22(3):243-247
[8] 刘金元, 刘大钧, 陶文静, 李万隆, 陈佩度. 小麦白粉病抗性基因Pm4a的RFLP标记转化为STS标记研究[J]. 农业生物技术学报, 1999, 7(2):113-116
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[10] Cenci A D, Ovidio R, Tanzarella O A. Identification of molecular markers linked to Pm13, an Aegilops long issima gene conferring resistance to powdery mildew in wheat[J]. Theoretical and Applied Genetics, 1999, 98(1):448-454
[11] 许立奎, 潘彬荣, 岳高红, 梅喜雪, 刘永安, 张宗宸, 周志辉. 抗白粉病糯性小麦的多重PCR分子鉴定技术[J]. 核农学报, 2014, 28(7):1203-1207
[12] 赵紫慧, 黄江, 陆鸣, 王晓鸣, 吴飞龙, 吴晓菲, 赵鑫, 李洪杰. 山东省和河北省小麦白粉病菌毒性与遗传多样性分析[J]. 作物学报, 2013, 39(1):1377-1385
[13] 江峥, 王琪琳, 吴建辉, 薛文波, 曾庆东, 黄丽丽, 康振生, 韩德俊. 基于基因特异性标记分析Pm21在中国冬小麦品种(系)中的分布[J]. 中国农业科学, 2014, 47(1):2078-2087
[14] 刘理森, 张倩, 任研, 顾晶晶, 詹克慧. 241份小麦品种(系)白粉病抗性鉴定与分子标记检测[J]. 分子植物育种, 2016, 14(3):619-637
[15] 刘兵, 李绍慧, 王永强, 胡东维. 我国主要小麦推广品种抗白粉病基因的分子检测[J]. 植物保护学报, 2010, 37(2):113-117
[16] Mohler Ⅴ, Jahoor A. Allele-specif ic amplification of polymorphic sites for the detection of powdery mildew resistance loci in wheat[J]. Theoretical and Applied Genetics, 1996, 93(1):1078-1082
[17] Tommasini L, Yahiaoui N, Srichumpa P, Keller B. Development of functional markers specific for seven Pm3 resistance alleles and their validation in the bread wheat gene pool[J]. Theoretical and Applied Genetics, 2006, 114(1):165-175
[18] Qi L L, Cao M Y, Chen P D, Li W L, Liu D J. Identification, mapping, and application of polymorphic DNA associated with resistance gene Pm21 of wheat[J]. Genome, 1996, 39(1):191-197
[19] 王汝锋, 李立会, 郑殿升, 吕波, 王晓鸣, 翁跃进, 杨金华. GB/T 19557.2-2004植物新品种特异性、一致性和稳定性测试指南普通小麦[S]. 北京:北京标准出版社,2004
[20] 季宏平, 孟庆林, 王芊, 张匀华, 石凤梅. 黑龙江省小麦白粉病菌毒性结构和毒力频率研究[J]. 黑龙江农业科学, 2007, 3(1):49-51
[21] 陈秀梅, 曹远银, 宋晶晶, 栾兆杰, 朱桂清. 我国部分麦区2011-2012年小麦白粉病菌小种及毒力分析[J]. 麦类作物学报, 2013, 33(3):584-588
[22] 李易初, 孟庆林, 石凤梅, 马立功, 刘佳. 2013年黑龙江省小麦白粉菌毒力结构分析[J]. 作物杂志, 2015, 167(4):20-22
[23] Liu Z, Sun Q. Development of SCAR markers linked to the Pm21 gene conferring resistance to powdery mildew in common wheat[J]. Plant Breeding, 1999, 118(1):215-219
[24] 齐莉莉, 陈佩度, 刘大钧, 周波, 张守中, 盛宝钦, 向齐君, 段霞渝, 周益林. 小麦白粉病新抗源-基因Pm21[J]. 作物学报, 1995, 21(3):257-262
[25] Bao Y G, Wu X, Zhang C, Li X F, He F, Qi X L, Wang H G. Chromosomal constitutions and reactions to powdery mildew and stripe rust of four novel wheat-Thinopyrum intermedium partial amphiploids[J]. Journal of Genetics and Genomics, 2014, 12(1):663-666
[26] Petersen S, Lyerly J H, Worthington M L, Parks W R, Cowger C, Marshall D S, Brown G, Murphy J P. Mapping of powdery mildew resistance gene Pm53 introgressed from Aegilops speltoides into soft red winter wheat[J]. Theoretical and Applied Genetics, 2015, 128(1):303-312
[27] 祁适雨, 肖志敏, 李仁杰. 中国东北强筋春小麦[J]. 中国农业出版社, 2006, 8(1):7-19
[28] 肖步阳. 春小麦生态育种[M]. 北京:中国农业出版社, 2006
[2] 赵远玲, 李祥羽, 孙连发, 陈立军. 人工合成小麦抗白粉病未知基因的SSR标记[J]. 植物遗传资源学报, 2011, 12(2):271-274
[3] 张增艳, 陈孝, 张超, 辛志勇, 陈新民. 分子标记选择小麦抗白粉病基因Pm4b、Pm13和Pm21聚合体[J]. 中国农业科学, 2002, 35(7):789-793
[4] 王玉叶, 张海萍, 潘艳秋, 常成, 马传喜. 96份小麦品种资源抗白粉病基因的分子检测[J]. 种子, 2013, 5(32):20-24
[5] 李祥羽, 孙连发, 陈立君, 宋凤英, 赵远玲. 小麦白粉病抗源筛选及抗源分布规律研究[J]. 黑龙江农业科学, 2009, 3(1):45-46
[6] 王丹, 赵继文, 秦海英, 谢文芳, 程星, 刘然方. 小麦抗白粉病基因定位及分子标记研究进展[J]. 农业科技通讯, 2013, 5(1):30-34
[7] 张宏纪, 刁艳玲, 孙连发, 孙岩, 刘东军, 郭强, 兰静, 黄景华, 杨淑萍, 孙光祖. 航天诱变新品种龙辐麦18的选育及其主要特征特性分析[J]. 核农学报, 2008, 22(3):243-247
[8] 刘金元, 刘大钧, 陶文静, 李万隆, 陈佩度. 小麦白粉病抗性基因Pm4a的RFLP标记转化为STS标记研究[J]. 农业生物技术学报, 1999, 7(2):113-116
[9] Francis H A, Leitch A R, Keobner R M. Conversion of a RAPD-generated PCR product, containing a novel dispersed repetitiveelement, into a fast and robust assay for thepresence of rye chromatin in wheat [J]. Theoreticaland Applied Genetics, 1995, 90(1):636-642
[10] Cenci A D, Ovidio R, Tanzarella O A. Identification of molecular markers linked to Pm13, an Aegilops long issima gene conferring resistance to powdery mildew in wheat[J]. Theoretical and Applied Genetics, 1999, 98(1):448-454
[11] 许立奎, 潘彬荣, 岳高红, 梅喜雪, 刘永安, 张宗宸, 周志辉. 抗白粉病糯性小麦的多重PCR分子鉴定技术[J]. 核农学报, 2014, 28(7):1203-1207
[12] 赵紫慧, 黄江, 陆鸣, 王晓鸣, 吴飞龙, 吴晓菲, 赵鑫, 李洪杰. 山东省和河北省小麦白粉病菌毒性与遗传多样性分析[J]. 作物学报, 2013, 39(1):1377-1385
[13] 江峥, 王琪琳, 吴建辉, 薛文波, 曾庆东, 黄丽丽, 康振生, 韩德俊. 基于基因特异性标记分析Pm21在中国冬小麦品种(系)中的分布[J]. 中国农业科学, 2014, 47(1):2078-2087
[14] 刘理森, 张倩, 任研, 顾晶晶, 詹克慧. 241份小麦品种(系)白粉病抗性鉴定与分子标记检测[J]. 分子植物育种, 2016, 14(3):619-637
[15] 刘兵, 李绍慧, 王永强, 胡东维. 我国主要小麦推广品种抗白粉病基因的分子检测[J]. 植物保护学报, 2010, 37(2):113-117
[16] Mohler Ⅴ, Jahoor A. Allele-specif ic amplification of polymorphic sites for the detection of powdery mildew resistance loci in wheat[J]. Theoretical and Applied Genetics, 1996, 93(1):1078-1082
[17] Tommasini L, Yahiaoui N, Srichumpa P, Keller B. Development of functional markers specific for seven Pm3 resistance alleles and their validation in the bread wheat gene pool[J]. Theoretical and Applied Genetics, 2006, 114(1):165-175
[18] Qi L L, Cao M Y, Chen P D, Li W L, Liu D J. Identification, mapping, and application of polymorphic DNA associated with resistance gene Pm21 of wheat[J]. Genome, 1996, 39(1):191-197
[19] 王汝锋, 李立会, 郑殿升, 吕波, 王晓鸣, 翁跃进, 杨金华. GB/T 19557.2-2004植物新品种特异性、一致性和稳定性测试指南普通小麦[S]. 北京:北京标准出版社,2004
[20] 季宏平, 孟庆林, 王芊, 张匀华, 石凤梅. 黑龙江省小麦白粉病菌毒性结构和毒力频率研究[J]. 黑龙江农业科学, 2007, 3(1):49-51
[21] 陈秀梅, 曹远银, 宋晶晶, 栾兆杰, 朱桂清. 我国部分麦区2011-2012年小麦白粉病菌小种及毒力分析[J]. 麦类作物学报, 2013, 33(3):584-588
[22] 李易初, 孟庆林, 石凤梅, 马立功, 刘佳. 2013年黑龙江省小麦白粉菌毒力结构分析[J]. 作物杂志, 2015, 167(4):20-22
[23] Liu Z, Sun Q. Development of SCAR markers linked to the Pm21 gene conferring resistance to powdery mildew in common wheat[J]. Plant Breeding, 1999, 118(1):215-219
[24] 齐莉莉, 陈佩度, 刘大钧, 周波, 张守中, 盛宝钦, 向齐君, 段霞渝, 周益林. 小麦白粉病新抗源-基因Pm21[J]. 作物学报, 1995, 21(3):257-262
[25] Bao Y G, Wu X, Zhang C, Li X F, He F, Qi X L, Wang H G. Chromosomal constitutions and reactions to powdery mildew and stripe rust of four novel wheat-Thinopyrum intermedium partial amphiploids[J]. Journal of Genetics and Genomics, 2014, 12(1):663-666
[26] Petersen S, Lyerly J H, Worthington M L, Parks W R, Cowger C, Marshall D S, Brown G, Murphy J P. Mapping of powdery mildew resistance gene Pm53 introgressed from Aegilops speltoides into soft red winter wheat[J]. Theoretical and Applied Genetics, 2015, 128(1):303-312
[27] 祁适雨, 肖志敏, 李仁杰. 中国东北强筋春小麦[J]. 中国农业出版社, 2006, 8(1):7-19
[28] 肖步阳. 春小麦生态育种[M]. 北京:中国农业出版社, 2006