Genetic mapping and expressivity of a wheat multi-pistil gene in mutant 12TP
|
摘要
We identified a wheat(Triticum aestivum L.) multi-pistil mutant from an F_2 breeding population in 2012, named 12 TP(three pistils in one floret). Genetic analysis showed that one dominant gene locus controlled the multi-pistil trait. Using homozygous normal and multi-pistil lines(near-isogenic lines; NILs) derived from the original mutant 12 TP, a simple sequence repeat(SSR) marker assay located the 12 TP locus on chromosome arm 2 DL. Four SSR markers were linked to 12 TP and their order was Xcfd233→Xcfd62-12 TP→Xwmc41→Xcfd168 at 15.85, 10.47, 2.89, and 10.37 cM, respectively. The average genetic expressivity of the trait ‘three pistils in one floret' was more than 98% in seven homozygous 12 TP lines; however, the average genetic expressivity in heterozygous F_1 plants was about 49%. Thus, the 12 TP is a semi-dominant gene locus, which differ from all previously reported multi-pistil mutants. Mutant 12 TP is a new useful germplasm for study of wheat floral development and for breeding of high yield wheat.
We identified a wheat(Triticum aestivum L.) multi-pistil mutant from an F_2 breeding population in 2012, named 12 TP(three pistils in one floret). Genetic analysis showed that one dominant gene locus controlled the multi-pistil trait. Using homozygous normal and multi-pistil lines(near-isogenic lines; NILs) derived from the original mutant 12 TP, a simple sequence repeat(SSR) marker assay located the 12 TP locus on chromosome arm 2 DL. Four SSR markers were linked to 12 TP and their order was Xcfd233→Xcfd62-12 TP→Xwmc41→Xcfd168 at 15.85, 10.47, 2.89, and 10.37 cM, respectively. The average genetic expressivity of the trait ‘three pistils in one floret' was more than 98% in seven homozygous 12 TP lines; however, the average genetic expressivity in heterozygous F_1 plants was about 49%. Thus, the 12 TP is a semi-dominant gene locus, which differ from all previously reported multi-pistil mutants. Mutant 12 TP is a new useful germplasm for study of wheat floral development and for breeding of high yield wheat.
We identified a wheat(Triticum aestivum L.) multi-pistil mutant from an F_2 breeding population in 2012, named 12 TP(three pistils in one floret). Genetic analysis showed that one dominant gene locus controlled the multi-pistil trait. Using homozygous normal and multi-pistil lines(near-isogenic lines; NILs) derived from the original mutant 12 TP, a simple sequence repeat(SSR) marker assay located the 12 TP locus on chromosome arm 2 DL. Four SSR markers were linked to 12 TP and their order was Xcfd233→Xcfd62-12 TP→Xwmc41→Xcfd168 at 15.85, 10.47, 2.89, and 10.37 cM, respectively. The average genetic expressivity of the trait ‘three pistils in one floret' was more than 98% in seven homozygous 12 TP lines; however, the average genetic expressivity in heterozygous F_1 plants was about 49%. Thus, the 12 TP is a semi-dominant gene locus, which differ from all previously reported multi-pistil mutants. Mutant 12 TP is a new useful germplasm for study of wheat floral development and for breeding of high yield wheat.
引文
Chen J S,Zhang L H,Wu B L.1983.A preliminary report on the discovery and breeding of the‘tri-grain wheat’.Acta Agronomica Sinica,9,67-71.(in Chinese)
Cota-Sánchez J H,Remarchuk K,Ubayasena K.2006.Readyto-use DNA extracted with a CTAB method adapted for herbarium specimens and mucilaginous plant tissue.Plant Molecular Biology Reports,24,161-167.
Cui J M,Guo T C,Zhu Y J,Wang C Y,Ma X M,Wang Y H,Wang H C,Yu H,Zhao R X,Ma D Y,Ji T H,Duan Z Q,Ji L F,Li L,Qi G C,He D X,Yuan T,Li C M,Zhao H J,Kang G Z.2008.The Spike of Wheat.China Agriculture Press,China.(in Chinese)
Duan Z B,Shen C C,Li Q Y,Lv G Z,Ni Y J,Yu D Y,Niu J S.2015.Identification of a novel male sterile wheat mutant dms conferring dwarf status and multi-pistils.Journal of Integrative Agriculture,14,1706-1714.
Endo T R,Gill B S.1996.The deletion stocks of common wheat.Journal of Heredity,87,295-307.
Gardner J S,Hess W M,Trione E J.1985.Development of the young wheat spike:A SEM study of Chinese Spring wheat.American Journal of Botany,72,548-559.
Gupta P,Balyan H,Edwards K,Isaac P,Korzun V,R?der M,Gautier M,Joudrier P,Schlatter A,Dubcovsky J,De la Pena R,Khairallah M,Penner G,Hayden M,Sharp P,Keller B,Wang R,Hardouin J,Jack P,Leroy P.2002.Genetic mapping of 66 new microsatellite(SSR)loci in bread wheat.Theoretical and Applied Genetics,105,413-422.
Guyomarc’h H,Sourdille P,Edwards K,Rernard M.2002.Studies of the transferability of microsatellites derived from Triticum tauschii to hexaploid wheat and to diploid related species using amplification,hybridization and sequence comparisons.Theoretical and Applied Genetics,105,736-744.
He R L,Chang Z J,Yang Z J,Yuan Z Y,Zhan H X,Zhang X J,Liu J X.2009.Inheritance and mapping of powdery mildew resistance gene Pm43 introgressed from Thinopyrum intermedium into wheat.Theoretical and Applied Genetics,118,1173-1180.
Kosambi D D.1944.The estimation of map distances from recombination values.The Annals of Eugenics,12,172-175.
Ma S C,Zhang G S,Li H R,Zhao C S.2006.Genetic analysis of multi-ovary character of wheat line Duo II.Journal of Triticeae Crops,26,35-37.(in Chinese)
Ma S C,Zhang G S,Liu H W,Wang J W,Wang X L.2000.Studies on the application of multi-ovary character to hybrid wheat.I.Multi-ovary gene loci and cytoplasm effect.Acta Botanica Boreali-Occidentalia Sinica,20,949-953.(in Chinese)
Niu J S,Wang B Q,Wang Y H,Cao A Z,Qi Z J,Shen T M.2008.Chromosome location and microsatellite markers linked to a powdery mildew resistance gene in wheat line‘Lankao 90(6)’.Plant Breeding,127,346-349.
Peng Z S.2003.A new mutation in wheat producing three pistils in a floret.Journal of Agronomy Crop Science,189,270-272.
Peng Z S,Martinek P,Kosuge K,Kuboyama T,Watanabe N.2008.Genetic mapping of a mutant gene producing three pistils per floret in common wheat.Journal of Applied Genetics,49,135-139.
Peng Z S,Yang Z Y,Ouyang Z M,Yang H.2013.Characterization of a novel pistillody mutant in common wheat.Australian Journal of Crop Science,7,159-164.
Pestsova E,Ganal M W,R?der M S.2000.Isolation and mapping of microsatellite markers specific for the D genome of bread wheat.Genome,43,689-697.
R?der M S,Korzun V,Wendehake K,Plaschke J,Tixier M H,Leroy P,Ganal M W.1998.A microsatellite map of wheat.Genetics,149,2007-2023.
Shen G H,Tong Y Z,Shen G Z.1992.Localization of the gene multi-ovary on chromosome and chromosome-arm of common wheat using monosomic and ditelosomic analysis.Acta Genetica Sinica,19,513-516.
Sears E R.1954.The aneuploids of common wheat.University of Missouri College of Agriculture Agricultural Experiment Station Research Bull,572,1-59.
Sibony M,Pinthus M J.1988.Floret initiation and development in spring wheat(Triticum aestivum L.).Annals of Botany,61,473-479.
Somers D J,Isaac P,Edwards K.2004.A high-density microsatellite consensus map for bread wheat(Triticum aestivum L.).Theoretical and Applied Genetics,109,1105-1114.
Song Q,Shi J,Singh S,Fickus E W,Costa J M,Lewis J,Gill BS,Ward R,Cregan P B.2005.Development and mapping of microsatellite(SSR)markers in wheat.Theoretical and Applied Genetics,110,550-560.
Sourdille P,Cadalen T,Guyomarc’h H,Snape J,Perretant M R,Charmet G,Boeuf C,Bernard S,Bernard M.2003.An update of the Courtot×Chinese Spring intervarietal molecular marker linkage map for the QTL detection of agronomic traits in wheat.Theoretical and Applied Genetics,106,530-538.
Srinivasan S,Gaur P M,Chaturvedi S K,Rao B V.2006.Allelic relationships of genes controlling number of flowers per axis in chickpea.Euphytica,152,331-337.
Tong Y Z,Tong P D.1984.Studies on multi-ovary in common wheat.I.The morphologenesis of multi-ovary in common wheat and the chromosomal location of its genes.Journal of Shanghai Normal University(Natural Sciences),2,48-53.(in Chinese)
Wang Z,Xu D,Ji J,Wang J,Wang M,Ling H,Sun G,Li J.2009.Genetic analysis and molecular markers associated with multi-gynoecia(Mg)gene in Trigrain wheat.Canadian Journal of Plant Science,89,845-850.
Wu J,Li B Q,Zhao J X.2000.Genetic analysis of multi-ovary character of trigrain wheat.The Journal of Northwest Agricultural University,28,58-60.(in Chinese)
Yang Z J,Chen Z Y,Peng Z S,Yu Y,Liao M L,Wei S H.2017.Development of a high-density linkage map and mapping of the three-pistil gene(Pis1)in wheat using GBS markers.BMC Genomics,18,567.
Zhu X X,Li Q Y,Shen C C,Duan Z B,Yu D Y,Niu J S,Ni YZ,Jiang Y M.2016.Transcriptome analysis for abnormal spike development of the wheat mutant dms.PLoS ONE,11,e0149287.
Cota-Sánchez J H,Remarchuk K,Ubayasena K.2006.Readyto-use DNA extracted with a CTAB method adapted for herbarium specimens and mucilaginous plant tissue.Plant Molecular Biology Reports,24,161-167.
Cui J M,Guo T C,Zhu Y J,Wang C Y,Ma X M,Wang Y H,Wang H C,Yu H,Zhao R X,Ma D Y,Ji T H,Duan Z Q,Ji L F,Li L,Qi G C,He D X,Yuan T,Li C M,Zhao H J,Kang G Z.2008.The Spike of Wheat.China Agriculture Press,China.(in Chinese)
Duan Z B,Shen C C,Li Q Y,Lv G Z,Ni Y J,Yu D Y,Niu J S.2015.Identification of a novel male sterile wheat mutant dms conferring dwarf status and multi-pistils.Journal of Integrative Agriculture,14,1706-1714.
Endo T R,Gill B S.1996.The deletion stocks of common wheat.Journal of Heredity,87,295-307.
Gardner J S,Hess W M,Trione E J.1985.Development of the young wheat spike:A SEM study of Chinese Spring wheat.American Journal of Botany,72,548-559.
Gupta P,Balyan H,Edwards K,Isaac P,Korzun V,R?der M,Gautier M,Joudrier P,Schlatter A,Dubcovsky J,De la Pena R,Khairallah M,Penner G,Hayden M,Sharp P,Keller B,Wang R,Hardouin J,Jack P,Leroy P.2002.Genetic mapping of 66 new microsatellite(SSR)loci in bread wheat.Theoretical and Applied Genetics,105,413-422.
Guyomarc’h H,Sourdille P,Edwards K,Rernard M.2002.Studies of the transferability of microsatellites derived from Triticum tauschii to hexaploid wheat and to diploid related species using amplification,hybridization and sequence comparisons.Theoretical and Applied Genetics,105,736-744.
He R L,Chang Z J,Yang Z J,Yuan Z Y,Zhan H X,Zhang X J,Liu J X.2009.Inheritance and mapping of powdery mildew resistance gene Pm43 introgressed from Thinopyrum intermedium into wheat.Theoretical and Applied Genetics,118,1173-1180.
Kosambi D D.1944.The estimation of map distances from recombination values.The Annals of Eugenics,12,172-175.
Ma S C,Zhang G S,Li H R,Zhao C S.2006.Genetic analysis of multi-ovary character of wheat line Duo II.Journal of Triticeae Crops,26,35-37.(in Chinese)
Ma S C,Zhang G S,Liu H W,Wang J W,Wang X L.2000.Studies on the application of multi-ovary character to hybrid wheat.I.Multi-ovary gene loci and cytoplasm effect.Acta Botanica Boreali-Occidentalia Sinica,20,949-953.(in Chinese)
Niu J S,Wang B Q,Wang Y H,Cao A Z,Qi Z J,Shen T M.2008.Chromosome location and microsatellite markers linked to a powdery mildew resistance gene in wheat line‘Lankao 90(6)’.Plant Breeding,127,346-349.
Peng Z S.2003.A new mutation in wheat producing three pistils in a floret.Journal of Agronomy Crop Science,189,270-272.
Peng Z S,Martinek P,Kosuge K,Kuboyama T,Watanabe N.2008.Genetic mapping of a mutant gene producing three pistils per floret in common wheat.Journal of Applied Genetics,49,135-139.
Peng Z S,Yang Z Y,Ouyang Z M,Yang H.2013.Characterization of a novel pistillody mutant in common wheat.Australian Journal of Crop Science,7,159-164.
Pestsova E,Ganal M W,R?der M S.2000.Isolation and mapping of microsatellite markers specific for the D genome of bread wheat.Genome,43,689-697.
R?der M S,Korzun V,Wendehake K,Plaschke J,Tixier M H,Leroy P,Ganal M W.1998.A microsatellite map of wheat.Genetics,149,2007-2023.
Shen G H,Tong Y Z,Shen G Z.1992.Localization of the gene multi-ovary on chromosome and chromosome-arm of common wheat using monosomic and ditelosomic analysis.Acta Genetica Sinica,19,513-516.
Sears E R.1954.The aneuploids of common wheat.University of Missouri College of Agriculture Agricultural Experiment Station Research Bull,572,1-59.
Sibony M,Pinthus M J.1988.Floret initiation and development in spring wheat(Triticum aestivum L.).Annals of Botany,61,473-479.
Somers D J,Isaac P,Edwards K.2004.A high-density microsatellite consensus map for bread wheat(Triticum aestivum L.).Theoretical and Applied Genetics,109,1105-1114.
Song Q,Shi J,Singh S,Fickus E W,Costa J M,Lewis J,Gill BS,Ward R,Cregan P B.2005.Development and mapping of microsatellite(SSR)markers in wheat.Theoretical and Applied Genetics,110,550-560.
Sourdille P,Cadalen T,Guyomarc’h H,Snape J,Perretant M R,Charmet G,Boeuf C,Bernard S,Bernard M.2003.An update of the Courtot×Chinese Spring intervarietal molecular marker linkage map for the QTL detection of agronomic traits in wheat.Theoretical and Applied Genetics,106,530-538.
Srinivasan S,Gaur P M,Chaturvedi S K,Rao B V.2006.Allelic relationships of genes controlling number of flowers per axis in chickpea.Euphytica,152,331-337.
Tong Y Z,Tong P D.1984.Studies on multi-ovary in common wheat.I.The morphologenesis of multi-ovary in common wheat and the chromosomal location of its genes.Journal of Shanghai Normal University(Natural Sciences),2,48-53.(in Chinese)
Wang Z,Xu D,Ji J,Wang J,Wang M,Ling H,Sun G,Li J.2009.Genetic analysis and molecular markers associated with multi-gynoecia(Mg)gene in Trigrain wheat.Canadian Journal of Plant Science,89,845-850.
Wu J,Li B Q,Zhao J X.2000.Genetic analysis of multi-ovary character of trigrain wheat.The Journal of Northwest Agricultural University,28,58-60.(in Chinese)
Yang Z J,Chen Z Y,Peng Z S,Yu Y,Liao M L,Wei S H.2017.Development of a high-density linkage map and mapping of the three-pistil gene(Pis1)in wheat using GBS markers.BMC Genomics,18,567.
Zhu X X,Li Q Y,Shen C C,Duan Z B,Yu D Y,Niu J S,Ni YZ,Jiang Y M.2016.Transcriptome analysis for abnormal spike development of the wheat mutant dms.PLoS ONE,11,e0149287.