Development and genetic analysis of wheat double substitution lines carrying Hordeum vulgare 2H and Thinopyrum intermedium 2Ai#2 chromosomes
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摘要
Thinopyrum intermedium and barley are two close relatives of wheat and carry many genes that are potentially valuable for the improvement of various wheat traits. In this study we created wheat double substitution lines by hybridizing different wheat–Th. intermedium and wheat–barley disomic alien substitution lines, with the aim of using genes in Th. intermedium and barley for wheat breeding and investigating the genetic behavior of alien chromosomes and their wheat homoeologs. As expected, we obtained two types of wheat double substitution lines,2D2Ai#2(2B)2H( A) and 2A2 Ai#2(2B)2H(2D), in which different group 2 wheat chromosomes were replaced by barley chromosome 2 H and Th. intermedium chromosome 2Ai#2. The new materials were characterized using molecular markers, genomic in situ hybridization(GISH), and fluorescent in situ hybridization(FISH). GISH and FISH experiments revealed that the double substitution lines harbor 42 chromosomes including 38 wheat chromosomes, a pair of barley chromosomes, and a pair of Th. intermedium chromosomes. Analysis using specific DNA markers showed that two pairs of wheat homoeologous group 2 chromosomes in the new lines were substituted by a pair of 2H and a pair of 2Ai#2 chromosomes. Chromosome 2H showed a higher transmission rate than 2Ai#2, and both chromosomes were preferentially transmitted between generations via female gametes. Evaluation of botanic and agronomic traits demonstrated that,compared with their parents, the new lines showed similar growth habits and plant type but differences in plant height, flowering date, and self-fertility. Cytological observations using different probes suggested that the double substitution lines showed nearly normal genetic behavior before and during meiosis. The novel substitution lines can potentially be used in wheat meiosis research and breeding programs.
Thinopyrum intermedium and barley are two close relatives of wheat and carry many genes that are potentially valuable for the improvement of various wheat traits. In this study we created wheat double substitution lines by hybridizing different wheat–Th. intermedium and wheat–barley disomic alien substitution lines, with the aim of using genes in Th. intermedium and barley for wheat breeding and investigating the genetic behavior of alien chromosomes and their wheat homoeologs. As expected, we obtained two types of wheat double substitution lines,2D2Ai#2(2B)2H( A) and 2A2 Ai#2(2B)2H(2D), in which different group 2 wheat chromosomes were replaced by barley chromosome 2 H and Th. intermedium chromosome 2Ai#2. The new materials were characterized using molecular markers, genomic in situ hybridization(GISH), and fluorescent in situ hybridization(FISH). GISH and FISH experiments revealed that the double substitution lines harbor 42 chromosomes including 38 wheat chromosomes, a pair of barley chromosomes, and a pair of Th. intermedium chromosomes. Analysis using specific DNA markers showed that two pairs of wheat homoeologous group 2 chromosomes in the new lines were substituted by a pair of 2H and a pair of 2Ai#2 chromosomes. Chromosome 2H showed a higher transmission rate than 2Ai#2, and both chromosomes were preferentially transmitted between generations via female gametes. Evaluation of botanic and agronomic traits demonstrated that,compared with their parents, the new lines showed similar growth habits and plant type but differences in plant height, flowering date, and self-fertility. Cytological observations using different probes suggested that the double substitution lines showed nearly normal genetic behavior before and during meiosis. The novel substitution lines can potentially be used in wheat meiosis research and breeding programs.
Thinopyrum intermedium and barley are two close relatives of wheat and carry many genes that are potentially valuable for the improvement of various wheat traits. In this study we created wheat double substitution lines by hybridizing different wheat–Th. intermedium and wheat–barley disomic alien substitution lines, with the aim of using genes in Th. intermedium and barley for wheat breeding and investigating the genetic behavior of alien chromosomes and their wheat homoeologs. As expected, we obtained two types of wheat double substitution lines,2D2Ai#2(2B)2H( A) and 2A2 Ai#2(2B)2H(2D), in which different group 2 wheat chromosomes were replaced by barley chromosome 2 H and Th. intermedium chromosome 2Ai#2. The new materials were characterized using molecular markers, genomic in situ hybridization(GISH), and fluorescent in situ hybridization(FISH). GISH and FISH experiments revealed that the double substitution lines harbor 42 chromosomes including 38 wheat chromosomes, a pair of barley chromosomes, and a pair of Th. intermedium chromosomes. Analysis using specific DNA markers showed that two pairs of wheat homoeologous group 2 chromosomes in the new lines were substituted by a pair of 2H and a pair of 2Ai#2 chromosomes. Chromosome 2H showed a higher transmission rate than 2Ai#2, and both chromosomes were preferentially transmitted between generations via female gametes. Evaluation of botanic and agronomic traits demonstrated that,compared with their parents, the new lines showed similar growth habits and plant type but differences in plant height, flowering date, and self-fertility. Cytological observations using different probes suggested that the double substitution lines showed nearly normal genetic behavior before and during meiosis. The novel substitution lines can potentially be used in wheat meiosis research and breeding programs.
引文
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[20]A.K.M.R.Islam,Wheat-barley addition lines:their use in genetic and evolutionary studies of barley,Barley Genetics IV:Proceedings of Fourth International Barley Genetics Symposium,1981.
[21]U.Akmr,K.W.Shepherd,Substituting ability of individual barley chromosomes for wheat chromosomes:1.Substitutions involving barley chromosomes 1,3 and 6,Plant Breed.109(1992)141-150.
[22]T.Efremova,V.Arbuzova,N.Trubacheeva,T.Ocadchaya,E.Chumanova,L.Pershina,Substitution of Hordeum marinum ssp.gussoneanum chromosome 7HL into wheat homoeologous group-7,Euphytica 192(2013)251-257.
[23]K.Shirasu,T.Lahaye,M.W.Tan,F.Zhou,C.Azevedo,P.Schulze-Lefert,A novel class of eukaryotic zinc-binding proteins is required for disease resistance signaling in barley and development in C.elegans,Cell 99(1999)355-366.
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[28]M.J.Wang,Y.Zhang,Z.S.Lin,X.G.Ye,Y.P.Yuan,W.Ma,Z.Y.Xin,Development of EST-PCR markers for Thinopyrum intermedium chromosome 2Ai#2 and their application in characterization of novel wheat-grass recombinants,Theor.Appl.Genet.121(2010)1369-1380.
[29]M.She,J.Wang,X.Wang,G.Yin,K.Wang,L.Du,X.Ye,Comprehensive molecular analysis of arginase-encoding genes in common wheat and its progenitor species,Sci.Rep.7(2017)6641.
[30]J.Yuan,X.Guo,J.Hu,Z.Lv,F.Han,Characterization of two CENH3 genes and their roles in wheat evolution,New Phytol.206(2015)839-851.
[31]X.Guo,H.Su,Q.Shi,S.Fu,J.Wang,X.Zhang,Z.Hu,F.Han,De novo centromere formation and centromeric sequence expansion in wheat and its wide hybrids,PLoS Genet.12(2016),e1005997.
[32]H.Yao,C.G.Tang,J.Zhao,Q.Zheng,B.Li,C.Y.Hao,Z.S.Li,X.Y.Zhang,Isolation of Thinopyrum ponticum genome specific repetitive sequences and their application for effective detection of alien segments in wheat,Sci.Agric.Sin.49(2016)3683-3693(in Chinese with English abstract).
[33]A.Sepsi,I.Molnár,D.Szalay,M.Molnárláng,Characterization of a leaf rust-resistant wheat-Thinopyrum ponticum partial amphiploid BE-1,using sequential multicolor GISH and FISH,Theor.Appl.Genet.116(2008)825-834.
[34]I.P.King,K.A.Purdie,H.N.Rezanoor,R.M.D.Koebner,T.E.Miller,S.M.Reader,P.Nicholson,Characterization of Thinopyrum bessarabicum chromosome segments in wheat using random amplified polymorphic DNAs(RAPDs)and genomic in situ hybridization,Theor.Appl.Genet.86(1993)895-900.
[35]X.Fei,W.Ji,C.Wang,Z.Hong,B.Yang,High-density mapping and marker development for the powdery mildew resistance gene PmAS846 derived from wild emmer wheat(Triticum turgidum var.dicoccoides),Theor.Appl.Genet.124(2012)1549-1560.
[36]R.Mcintosh,R.Park,P.Zhang,I.S.Dundas,B.Friebe,B.S.Gill,S.Xu,Wheat-Aegilops introgressions,in:M.Molnár-Láng,C.Ceoloni,J.Dole?el(Eds.),Alien Introgression in Wheat,Springer,Cham,Switzerland,2015.
[37]L.Song,Y.Lu,J.Zhang,C.Pan,X.Yang,X.Li,W.Liu,L.Li,Physical mapping of Agropyron cristatum chromosome 6Pusing deletion lines in common wheat background,Theor.Appl.Genet.129(2016)1023-1034.
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