胃癌组织中人巨细胞病毒感染及其临床意义
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摘要
目的:初步探讨人巨细胞病毒(HCMV)感染与胃癌的关系。方法:采用化学发光(CLIA)法对胃癌组(n=80)及健康对照组(n=80)的外周血清进行HCMV Ig G、Ig M抗体检测;巢式PCR技术检测22例胃癌患者的肿瘤及癌旁正常胃黏膜组织标本中的HCMV UL55基因;统计分析HCMV感染与胃癌临床特征的关系。结果:胃癌组血清HCMV-Ig G阳性率为95%(76/80),HCMV-Ig M阳性率为6.25%(5/80);在健康对照组血清中,HCMV-Ig G阳性率为97.5%(78/80),HCMV-Ig M阳性率为3.75%(3/80),HCMV Ig G、Ig M在胃癌组与健康对照组血清中差异无统计学意义(P>0.05)。HCMV UL55阳性检出率在22例胃癌组织中为50%(11/22),在相应正常胃黏膜组织中为9.09%(2/22),胃癌组织HCMV阳性检出率较癌旁正常组织显著升高(P<0.01)。此外,未发现HCMV感染与胃癌的TMN分期、组织病理分化及肿块大小有显著性关联。结论:胃癌组织中存在HCMV感染,且HCMV相较于癌旁正常组织更倾向感染癌灶,提示HCMV感染可能参与胃癌的发生发展过程。
Objective: To explore the correlation between HCMV infection and gastric cancer. Methods: The specific IgG and IgM antibodies of HCMV in serum of gastric cancer group(n=80) and health control group(n=80) were detected using the chemiluminesence immunoassay. HCMV infection in gastric cancer tissues and corresponding normal tissues were determined through the detection of UL55 gene by Nested PCR. The relationships between HCMV infection and clinical features of gastric cancer were further analyzed. Results: The positive rate of HCMV-IgG and IgM were 95%(76/80) and 6.25%(5/80) in gastric cancer group, respectively. Meanwhile, the positive rate were 97.5%(78/80) and 3.75%(3/80) in health control. There was no statistically significant difference between the gastric cancer and health control group in HCMV-IgG, IgM detection. However, there was a significant difference between gastric cancer tissues and corresponding normal tissues in HCMV detection. The positive rate of HCMV UL55 was 50%(11/22) in gastric cancer tissue samples, whereas 9.09%(2/22) in corresponding normal tissues through Nested PCR detection. In addition, There was no obvious association between HCMV infection and TMN stage, pathological differentiation type and the size of tumor. Conclusion: It is the first time to report the HCMV infection in gastric cancer tissues, and the HCMV may preferentially infect the neoplastic epithelium than corresponding normal epithelium, this research suggests the HCMV infection may participate in the occurrence and development stages of gastric cancer.
Objective: To explore the correlation between HCMV infection and gastric cancer. Methods: The specific IgG and IgM antibodies of HCMV in serum of gastric cancer group(n=80) and health control group(n=80) were detected using the chemiluminesence immunoassay. HCMV infection in gastric cancer tissues and corresponding normal tissues were determined through the detection of UL55 gene by Nested PCR. The relationships between HCMV infection and clinical features of gastric cancer were further analyzed. Results: The positive rate of HCMV-IgG and IgM were 95%(76/80) and 6.25%(5/80) in gastric cancer group, respectively. Meanwhile, the positive rate were 97.5%(78/80) and 3.75%(3/80) in health control. There was no statistically significant difference between the gastric cancer and health control group in HCMV-IgG, IgM detection. However, there was a significant difference between gastric cancer tissues and corresponding normal tissues in HCMV detection. The positive rate of HCMV UL55 was 50%(11/22) in gastric cancer tissue samples, whereas 9.09%(2/22) in corresponding normal tissues through Nested PCR detection. In addition, There was no obvious association between HCMV infection and TMN stage, pathological differentiation type and the size of tumor. Conclusion: It is the first time to report the HCMV infection in gastric cancer tissues, and the HCMV may preferentially infect the neoplastic epithelium than corresponding normal epithelium, this research suggests the HCMV infection may participate in the occurrence and development stages of gastric cancer.
引文
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[2]Soderberg-Naucler C.Does cytomegalovirus play a causative role in the development of various infl ammatory diseases and cancer?[J].J Intern Med,2006,259(3):219-246.
[3]Fisher RA.Cytomegalovirus infection and disease in the new era of immunosuppression following solid organ transplantation[J].Transpl Infect Dis,2009,11(3):195-202.
[4]Bego MG,St Jeor S.Human cytomegalovirus infection of cells of hematopoietic origin:HCMV-induced immunosuppression,immune evasion,and latency[J].Exp Hematol,2006,34(5):555-570.
[5]Samanta M,Harkins L,Klemm K,et al.High prevalence of human cytomegalovirus in prostatic intraepithelial neoplasia and prostatic carcinoma[J].J Urol,2003,170(3):998-1002.
[6]Cobbs CS,Harkins L,Samanta M,et al.Human cytomegalovirus infection and expression in human malignant glioma[J].Cancer Res,2002,62(12):3347-3350.
[7]Alkhawaja S,Ismaeel A,Botta G,et al.The prevalence of congenital and perinatal cytomegalovirus infections among newborns of seropositive mothers[J].J Infect Dev Ctries,2012,6(5):410-415.
[8]王永川,魏丽娟,刘俊田,等.发达与发展中国家癌症发病率与死亡率的比较与分析[J].中国肿瘤临床,2012,39(10):679-682.
[9]Scheurer ME,Bondy ML,Aldape KD,et al.Detection of human cytomegalovirus in different histological types of gliomas[J].Acta Neuropathol,2008,116(1):79-86.
[10]施浩强,葛俊,唐亮,等.HCMV活动性感染与尿路上皮癌的相关性研究[J].安徽医科大学学报,2013,48(1):60-65.
[11]Powell RD,Warner NE,Levine RS,et al.Cytomegalic inclusion disease and ulcerative colitis;report of a case in a young adult[J].Am J Med,1961,30(2):334-340.
[12]Ebisutani C,Kawamura A,Shibata N,et al.Gastric ulcer associated with cytomegalovirus in an immunocompetent patient:method for diagnosis[J].Case Rep Gastroenterol,2012,6(2):365-368.
[13]Al-Zafiri R,Gologan A,Galiatsatos P,et al.Cytomegalovirus complicating infl ammatory bowel disease:a 10-year experience in a community-based,university-affiliated hospital[J].Gastroenterol Hepatol(NY),2012,8(4):230-239.
[14]Hertel L,Lacaille VG,Strobl H,et al.Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus[J].J Virol,2003,77(13):7563-7574.
[15]Chen HP,Jiang JK,Chen CY,et al.Human cytomegalovirus preferentially infects the neoplastic epithelium of colorectal cancer:a quantitative and histological analysis[J].J Clin Virol,2012,54(3):240-244.
[16]Akintola-Ogunremi O,Luo Q,He TC,et al.Is cytomegalovirus associated with human colorectal tumorigenesis?[J].Am J Clin Patho,l 2005,123(2):244-249.
[17]Roubalova K,Strunecky O,Zufanova S,et al.Genotyping of viral glycoprotein B(g B)in hematopoietic stem cell transplant recipients with active cytomegalovirus infection:analysis of the impact of g B genotypes on the patients’outcome[J].Epidemiol Mikrobiol Imunol,2010,59(2):92-99.
[18]Michaelis M,Doerr HW,Cinatl J.The story of human cytomegalovirus and cancer:increasing evidence and open questions[J].Neoplasia,2009,11(1):1-9.
[19]Cinatl J Jr,Vogel JU,Kotchetkov R,et al.Oncomodulatory signals by regulatory proteins encoded by human cytomegalovirus:a novel role for viral infection in tumor progression[J].FEMS Microbiol Rev,2004,28(1):59-77.