T淋巴细胞亚群比值对HIV感染患者病情的判断价值分析
|
摘要
目的:探讨T淋巴细胞亚群比值对HIV感染患者病情的预测价值。方法:选择HIV感染无症状患者23例、艾滋病期患者82例为研究对象,15例体检健康患者为对照组,采取流式细胞术检测T淋巴细胞亚群,实时PCR法检测HIVRNA,对比分析CD4~+/CD3~+T、CD4~+/CD8~+T、CD8~+/CD3~+T。结果:CD4~+/CD3~+T、CD4~+/CD8~+T、CD8~+/CD3~+T预测艾滋病期的受试工作者特征曲线下面积为0.800、0.795、0.727(P<0.05);仅CD4~+/CD3~+T淋巴细胞≤0.14预测HIV感染患者进入艾滋病期比值比为19.677(P=0.011)。结论:CD4~+/CD3~+T可作为判断HIV感染患者是否进入艾滋病期的因素之一。
Objective:To explore the predictive value of the ratio of T lymphocyte subsets in patients with HIV infection. Methods:23 asymptomatic patents with HIV infection and 82 AIDS patients were enrolled in the studying,15 healthy persons were selected as control group. T lymphocyte subsets were detected by flow cytometry, HIV RNA was detected by real-time polymerase chain reaction,analysis CD_4~+/CD_3~+T、CD_4~+/CD_8~+T、CD_8~+/CD_3~+T. Results:The area under of the ROC curve of CD_4~+/CD_3~+T、CD_4~+/CD_8~+T、CD_8~+/CD_3~+T predicting AIDS was 0.800、0.795、0.72(7 P<0.05)respectively. But only the odds ratio was 19.677 predicting AIDS when the ratio of CD_4~+/CD_3~+T was below 0.14(P=0.011). Conclusion:The ratio of CD_4~+/CD_3~+T could be used to determine whether HIV patients entered AIDS period.
Objective:To explore the predictive value of the ratio of T lymphocyte subsets in patients with HIV infection. Methods:23 asymptomatic patents with HIV infection and 82 AIDS patients were enrolled in the studying,15 healthy persons were selected as control group. T lymphocyte subsets were detected by flow cytometry, HIV RNA was detected by real-time polymerase chain reaction,analysis CD_4~+/CD_3~+T、CD_4~+/CD_8~+T、CD_8~+/CD_3~+T. Results:The area under of the ROC curve of CD_4~+/CD_3~+T、CD_4~+/CD_8~+T、CD_8~+/CD_3~+T predicting AIDS was 0.800、0.795、0.72(7 P<0.05)respectively. But only the odds ratio was 19.677 predicting AIDS when the ratio of CD_4~+/CD_3~+T was below 0.14(P=0.011). Conclusion:The ratio of CD_4~+/CD_3~+T could be used to determine whether HIV patients entered AIDS period.
引文
[1]中华医学会感染病学分会艾滋病学组.艾滋病诊疗指南第三版(2015版)[J].中华临床感染病杂志,20158(5):85~401.
[2]Santagostino,A.G.Garbaccio,A.Pistorio,et al An Italian national multicenter study for the definition of reference ranges for normal values of periphera blood lymphocyte subsets in healthy adults[J]Haematologica,1999,84(6):499~504.
[3]Attili,V.S,S.Sundar,V.P.Singh,et al.Validity of existing CD4+classification in north Indians,in predicting immune status[J].J Infect,2005,51(1):41~46.
[4]Ghate,M V,S.M.Mehendale,B.A.Mahajan,e al.Relationship between clinical conditions and CD4counts in HIV-infected persons in Pune,Maharashtra India[J].Natl Med J India,2000,13(4):183~187.
[5]Jiao,Y,W.Hua,T.Zhang,et al.Characteristics of CD8+T cell subsets in Chinese patients with chronic HIV infection during initial ART[J].AIDSRes Ther,2011(8):15.
[6]Singh,H.R,N.G.Singh,T.B.Singh.Estimation of CD4+and CD8+T-lymphocytes in human immunodeficiency virus infection and acquired immunodeficiency syndrome patients in Manipur[J]Indian J Med Microbiol,2007,25(2):126~132.
[7]Hussain,T,K.K.Kulshreshtha,V.S.Yadav,et al CD4+,CD8+,CD3+cell counts and CD4+/CD8+ratio among patients with mycobacterial diseases(leprosy tuberculosis),HIV infections,and normal healthy adults:a comparative analysis of studies in differen regions of India[J].J Immunoassay Immunochem2015,36(4):420~443.
[2]Santagostino,A.G.Garbaccio,A.Pistorio,et al An Italian national multicenter study for the definition of reference ranges for normal values of periphera blood lymphocyte subsets in healthy adults[J]Haematologica,1999,84(6):499~504.
[3]Attili,V.S,S.Sundar,V.P.Singh,et al.Validity of existing CD4+classification in north Indians,in predicting immune status[J].J Infect,2005,51(1):41~46.
[4]Ghate,M V,S.M.Mehendale,B.A.Mahajan,e al.Relationship between clinical conditions and CD4counts in HIV-infected persons in Pune,Maharashtra India[J].Natl Med J India,2000,13(4):183~187.
[5]Jiao,Y,W.Hua,T.Zhang,et al.Characteristics of CD8+T cell subsets in Chinese patients with chronic HIV infection during initial ART[J].AIDSRes Ther,2011(8):15.
[6]Singh,H.R,N.G.Singh,T.B.Singh.Estimation of CD4+and CD8+T-lymphocytes in human immunodeficiency virus infection and acquired immunodeficiency syndrome patients in Manipur[J]Indian J Med Microbiol,2007,25(2):126~132.
[7]Hussain,T,K.K.Kulshreshtha,V.S.Yadav,et al CD4+,CD8+,CD3+cell counts and CD4+/CD8+ratio among patients with mycobacterial diseases(leprosy tuberculosis),HIV infections,and normal healthy adults:a comparative analysis of studies in differen regions of India[J].J Immunoassay Immunochem2015,36(4):420~443.