宁夏产甘草不同组织部位成分差异比较
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摘要
目的:比较甘草木质部、韧皮部及木栓层指标成分含量差异,并研究其相关关系。方法:采用HPLC及紫外分光光度法测定宁夏产甘草及其木栓层、韧皮部、木质部中甘草苷、甘草酸和总黄酮含量,利用R3.2.3软件进行方差分析及相关性分析。结果:甘草组织构成以木质部和韧皮部为主,有少量木栓层存在;甘草中总黄酮主要分布在木栓层;甘草苷主要分布在木质部和韧皮部;甘草酸在木质部、韧皮部和木栓层均匀分布;木栓层总黄酮与甘草中总黄酮含量呈极显著正相关;木质部、韧皮部、木栓层,中甘草苷含量与甘草中甘草苷含量呈显著相关(P<0.01,P<0.05),木质部和韧皮部中甘草酸含量与甘草中甘草酸含量显著相关(P<0.01,P<0.05);木质部和韧皮部甘草酸含量与其质量百分比呈显著负相关(P<0.01,P<0.05)。结论:本研究结果为研究甘草中甘草酸、甘草苷的生物合成及总黄酮构成做了铺垫,为甘草研究及利用提供了新的思路。
Objective: To compare the components differences among xylem, phloem, and phellem layer of Radix Glycyrrhizae, and study the correlation between them. Methods: HPLC and ultraviolet spectrophotometry were performed to determine the liquiritin, glycyrrhizic acid and total flavonoids content of xylem, phloem and phellem layers of Radix Glycyrrhizae,respectively. R 3.2.3 software was used in variance analysis and correlation analysis. Results: Radix Glycyrrhizae was mainly composed of xylem and phloem, with a few phellem layer. Total flavonoids were mainly distributed in the phellem layer. Liquiritin were mainly distributed in the xylem and phloem. Glycyrrhizic acid was equidistributed in the xylem, phloem and phellem layer.There was a significant positive correlation between total flavonoids in cork layer and total flavonoids in licorice. The content of glycyrrhizin in xylem and phloem was significantly correlated with the content of glycyrrhizin in licorice, while that in cork layer was significantly correlated(P<0.01, P<0.05). The content of glycyrrhizin in xylem and phloem was significantly and significantly correlated with the content of glycyrrhizin licorice, respectively(P<0.01, P<0.05). The content of glycyrrhizin xylem and phloem was significantly negatively correlated with the content of glycyrrhizin licorice, and positively correlated with the content of glycyrrhizin phloem(P<0.01, P<0.05). Conclusion: The results of this study pave the way for the study of the biosynthesis of glycyrrhizic acid and glycyrrhizin and the composition of total flavonoids in Radix Glycyrrhizae, and provide a new thought for the research and utilization of Radix Glycyrrhizae.
Objective: To compare the components differences among xylem, phloem, and phellem layer of Radix Glycyrrhizae, and study the correlation between them. Methods: HPLC and ultraviolet spectrophotometry were performed to determine the liquiritin, glycyrrhizic acid and total flavonoids content of xylem, phloem and phellem layers of Radix Glycyrrhizae,respectively. R 3.2.3 software was used in variance analysis and correlation analysis. Results: Radix Glycyrrhizae was mainly composed of xylem and phloem, with a few phellem layer. Total flavonoids were mainly distributed in the phellem layer. Liquiritin were mainly distributed in the xylem and phloem. Glycyrrhizic acid was equidistributed in the xylem, phloem and phellem layer.There was a significant positive correlation between total flavonoids in cork layer and total flavonoids in licorice. The content of glycyrrhizin in xylem and phloem was significantly correlated with the content of glycyrrhizin in licorice, while that in cork layer was significantly correlated(P<0.01, P<0.05). The content of glycyrrhizin in xylem and phloem was significantly and significantly correlated with the content of glycyrrhizin licorice, respectively(P<0.01, P<0.05). The content of glycyrrhizin xylem and phloem was significantly negatively correlated with the content of glycyrrhizin licorice, and positively correlated with the content of glycyrrhizin phloem(P<0.01, P<0.05). Conclusion: The results of this study pave the way for the study of the biosynthesis of glycyrrhizic acid and glycyrrhizin and the composition of total flavonoids in Radix Glycyrrhizae, and provide a new thought for the research and utilization of Radix Glycyrrhizae.
引文
[1]国家药典委员会.中华人民共和国药典一部.北京:中国医药科技出版社,2015:86-87
[2]杨路路,陈建军,杨天顺,等.我国西北地区药用植物甘草(Glycyrrhizauralensis)野生资源的地理分布与调查.中国野生植物资源,2013,32(5):27-31
[3]黄明进,王文全,魏胜利.我国甘草药用植物资源调查及质量评价研究.中国中药杂志,2010,35(8):947-952
[4]李学斌,陈林,李国旗,等.中国甘草资源的生态分布及其繁殖技术研究.生态环境学报,2013,22(4):718-722
[5]杨晓露,刘朵,卞卡,等.甘草总黄酮及其成分体外抗炎活性及机制研究.中国中药杂志,2013,38(1):99-104
[6]郑君,林晓春,陈育尧,等.甘草总黄酮抑制慢性萎缩性胃炎大鼠胃黏膜腺体萎缩及机制研究.中国药理学通报,2014,30(1):113-117
[7]林晓春,陈育尧,白殊同,等.甘草总黄酮对慢性浅表性胃炎大鼠胃粘膜损伤的保护作用.南方医科大学学报,2013,33(2):299-304
[8]陈云华,黄明进,王文全,等.不同来源甘草药材成分含量及其保肝降酶作用比较.中国实验方剂学杂志,2013,19(18):113-116
[9]韩亚男,张学静,侯俊玲,等.野生和栽培甘草不同器官中总黄酮和总多糖的动态积累规律研究.北京中医药大学学报,2014,7(5):344-348
[10]胡正海.药用植物的结构、发育及其主要药用成分积累的关系.中草药,2009,40(增刊):14-17
[11]马玲,安瑜,王坤,等.甘草中总黄酮含量测定的方法学研究.宁夏医学杂志,2010,32(5):428-429
[12]廖云海,陆嘉惠,李娜,等.光果甘草营养器官结构及其总黄酮的组织化学定位和含量研究.西北植物学报,2010,30(12):2406-2411
[2]杨路路,陈建军,杨天顺,等.我国西北地区药用植物甘草(Glycyrrhizauralensis)野生资源的地理分布与调查.中国野生植物资源,2013,32(5):27-31
[3]黄明进,王文全,魏胜利.我国甘草药用植物资源调查及质量评价研究.中国中药杂志,2010,35(8):947-952
[4]李学斌,陈林,李国旗,等.中国甘草资源的生态分布及其繁殖技术研究.生态环境学报,2013,22(4):718-722
[5]杨晓露,刘朵,卞卡,等.甘草总黄酮及其成分体外抗炎活性及机制研究.中国中药杂志,2013,38(1):99-104
[6]郑君,林晓春,陈育尧,等.甘草总黄酮抑制慢性萎缩性胃炎大鼠胃黏膜腺体萎缩及机制研究.中国药理学通报,2014,30(1):113-117
[7]林晓春,陈育尧,白殊同,等.甘草总黄酮对慢性浅表性胃炎大鼠胃粘膜损伤的保护作用.南方医科大学学报,2013,33(2):299-304
[8]陈云华,黄明进,王文全,等.不同来源甘草药材成分含量及其保肝降酶作用比较.中国实验方剂学杂志,2013,19(18):113-116
[9]韩亚男,张学静,侯俊玲,等.野生和栽培甘草不同器官中总黄酮和总多糖的动态积累规律研究.北京中医药大学学报,2014,7(5):344-348
[10]胡正海.药用植物的结构、发育及其主要药用成分积累的关系.中草药,2009,40(增刊):14-17
[11]马玲,安瑜,王坤,等.甘草中总黄酮含量测定的方法学研究.宁夏医学杂志,2010,32(5):428-429
[12]廖云海,陆嘉惠,李娜,等.光果甘草营养器官结构及其总黄酮的组织化学定位和含量研究.西北植物学报,2010,30(12):2406-2411