藤龙补中汤对大肠癌RKO细胞癌药靶基因表达的影响
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摘要
目的 观察藤龙补中汤对人大肠癌RKO细胞癌药靶基因表达影响。方法 藤龙补中汤作用RKO细胞,TRIzol试剂提取RNA,试剂盒纯化RNA,药靶基因PCR芯片(Cancer Drug Targets PCR Array)检测基因表达。结果 藤龙补中汤作用RKO细胞后,PLK3和TP53表达升高,ABCC1、AURKA、BCL2、BIRC5、CDK2、CDK4、FLT4、HDAC1、HIF1A、MDM4、MTOR、NFKB1和PLK1表达降低,涉及细胞增殖、细胞周期、凋亡、细胞衰老、多药耐药、转移、淋巴管生成和表观遗传修饰。结论 藤龙补中汤可调控RKO细胞多个癌药靶基因表达,与藤龙补中汤对大肠癌细胞增殖、细胞周期、凋亡、细胞衰老、转移和表观遗传作用相关;藤龙补中汤还可能对大肠癌多药耐药和淋巴管生成有抑制作用。
Objective To observe the effects of Teng-Long-Bu-Zhong-Tang(TLBZT) on expression of cancer drug target genes in human colorectal carcinoma RKO cells. Methods RKO cells were treated with TLBZT. RNA was extracted by TRIzol regent and purified with commercial kit. Genes expression were detected by Cancer Drug Targets PCR array. Results After TLBZT treatment, PLK3 and TP53 were up-regulated,ABCC1, AURKA, BCL2, BIRC5, CDK2, CDK4, FLT4, HDAC1, HIF1 A, MDM4, MTOR, NFKB1 and PLK1 were down-regulated in RKO cells. These genes involve cell proliferation, cell cycle, apoptosis, cell senescence, multidrug resistance, metastasis, lymphoangiogenesis and epigenetic modification. Conclusion TLBZT regulates expression of multiple genes of cancer drug targets and are related to the effects of TLBZT on cell proliferation, cell cycle, apoptosis, cell senescence, metastasis and epigenetics in colorectal cancer. TLBZT may also inhibit lymphoangiogenesis and multidrug resistance in colorectal cancer.
Objective To observe the effects of Teng-Long-Bu-Zhong-Tang(TLBZT) on expression of cancer drug target genes in human colorectal carcinoma RKO cells. Methods RKO cells were treated with TLBZT. RNA was extracted by TRIzol regent and purified with commercial kit. Genes expression were detected by Cancer Drug Targets PCR array. Results After TLBZT treatment, PLK3 and TP53 were up-regulated,ABCC1, AURKA, BCL2, BIRC5, CDK2, CDK4, FLT4, HDAC1, HIF1 A, MDM4, MTOR, NFKB1 and PLK1 were down-regulated in RKO cells. These genes involve cell proliferation, cell cycle, apoptosis, cell senescence, multidrug resistance, metastasis, lymphoangiogenesis and epigenetic modification. Conclusion TLBZT regulates expression of multiple genes of cancer drug targets and are related to the effects of TLBZT on cell proliferation, cell cycle, apoptosis, cell senescence, metastasis and epigenetics in colorectal cancer. TLBZT may also inhibit lymphoangiogenesis and multidrug resistance in colorectal cancer.
引文
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[2] 胡兵,安红梅,沈克平,等.藤龙补中汤促结肠癌LS-174T细胞衰老及其机制[J].中西医结合学报,2010,8(11):1048.
[3] 胡兵,安红梅,郑佳露,等.藤龙补中汤促人结肠癌RKO细胞衰老及其可能的作用机制[J].肿瘤,2016,36(12):1307.
[4] Hu B,An HM,Wang SS,et al.Teng-Long-Bu-Zhong-Tang induces p21-dependent cell senescence in colorectal carcinoma LS174T cells via histone acetylation [J].J Exp Pharmacol,2017,9:67.
[5] Deng S,Hu B,An HM,et al.Teng-Long-Bu-Zhong-Tang,a Chinese herbal formula,enhances anticancer effects of 5-Fluorouracil in CT26 colon carcinoma [J].BMC Complement Altern Med,2013,13(1):128.
[6] 邓珊,安红梅,胡兵.藤龙补中汤对大肠癌肺转移及肿瘤相关巨噬细胞作用[J].中国中西医结合消化杂志,2016,24(7):515.
[7] Wei MM,Wang SS,Zheng JL,et al.Herbal compound Teng-Long-Bu-Zhong-Tang inhibits metastasis of Human RKO colon carcinoma [J].Oncol Lett,2017,14(6):7767.
[8] 胡兵,安红梅,郑佳露,等.藤龙补中汤对大肠癌RKO细胞转移相关基因表达影响[J].中国中西医结合消化杂志,2018,26(5):416.
[9] Francipane MG,Lagasse E.mTOR pathway in colorectal cancer:an update [J].Oncotarget,2014,5(1):49.
[10] Han DP,Zhu QL,Cui JT,et al.Polo-like kinase 1 is overexpressed in colorectal cancer and participates in the migration and invasion of colorectal cancer cells [J].Med Sci Monit,2012,18(6):BR237.
[11] Klauck PJ,Bagby SM,Capasso A,et al.Antitumor activity of the polo-like kinase inhibitor,TAK-960,against preclinical models of colorectal cancer [J].BMC Cancer,2018,18(1):136.
[12] Takai N,Hamanaka R,Yoshimatsu J,et al.Polo-like kinases(Plks) and cancer[J].Oncogene,2005,24(2):287.
[13] Lv H,Gao G,Zhang L,Sun Y.Polo-like kinase 3 inhibits osteosarcoma cell proliferation and tumorigenesis via cooperative interaction with p21 [J].Mol Med Rep,2015,12(5):6789.
[14] Cao D,Qin S,Mu Y,Zhong M.The role of MRP1 in the multidrug resistance of colorectal cancer [J].Oncol Lett,2017,13(4):2471.